Decrease In Respiratory Sinus Arrhythmia Research Articles

Physiological regulation in infants of women with a mood disorder: examining associations with maternal symptoms and stress

The offspring of mothers with mood disorders may evidence increased behavioral problems as early as preschool; however, no study to date has examined psychophysiological characteristics during infancy, particularly among offspring of mothers diagnosed with bipolar disorder. Elucidating psychobiological mechanisms of risk early in development is critical to inform prevention and early intervention efforts. This study compared physiological and behavioral responsivity in 6-month-old infants (N = 329) of mothers with lifetime histories of bipolar disorder (BD, n = 44), major depressive disorder (MDD, n = 244), or no history of Axis I disorders (CTL, n = 41). Infant respiratory sinus arrhythmia (RSA) was measured in a laboratory stressor paradigm. Measures of infant affect and behavior during mother-infant interaction, current maternal depressive symptoms, and exposure to stressful life events were examined with respect to diagnostic group and RSA. Groups did not differ in baseline RSA or infant affect measures. However, during the stressor task, infants of mothers with BD evidenced increases in RSA, while infants of MDD and CTL mothers evidenced decreases in RSA. Though levels of postnatal stress and current levels of maternal depressive symptoms differed among groups, neither of these factors predicted infant psychophysiological responses. At 6 months of age, infants of mothers with BD show differences in psychophysiological regulation. These differences cannot be accounted for by perinatal outcome, current maternal depressive symptoms, or exposure to stressful life events, and thus may reflect endophenotypic markers of psychopathological risk.

School-Aged Children's Depressive Rumination is Associated with Their Reactivity to Sadness but not Fear

It is well documented that a ruminative response style is associated with greater risk for depression in children and adults. Less is known about the association between rumination and stress reactivity, particularly among children. Similarly, the extent to which depressive rumination is associated with general reactivity to negative emotion, or more specifically to sadness, has not received sufficient attention. The current study examines the association between depressive rumination and stress reactivity in response to a mild laboratory stressor in school-aged children. A diverse sample of 94 children between the ages of 8 and 12 participated in this 2-session study in which they reported on their tendency to engage in depressive rumination. Children's cardiovascular reactivity (operationalized as respiratory sinus arrhythmia) was assessed while they completed a task in which they read vignettes depicting children experiencing sadness and fear. Children also reported on their emotional reaction to the sad and fear vignettes, and we assessed the length of time it took them to respond to these questions (reaction time). Rumination was associated with greater decreases in respiratory sinus arrhythmia and greater increases in self-reported negative emotion in response to the sad but not the fear vignettes, suggesting that children higher in depressive rumination experienced more subjective arousal and showed evidence of greater regulatory effort when contemplating sadness. Rumination was associated with slower reaction time to both types of vignettes in one condition of the paradigm only. Results are discussed in terms of their implications for rumination and stress reactivity.

Cardiac Vagal Tone and Quality of Parenting Show Concurrent and Time-Ordered Associations That Diverge in Abusive, Neglectful, and Non-Maltreating Mothers.

Concurrent and lagged maternal respiratory sinus arrhythmia (RSA) was monitored in the context of parenting. One hundred and forty-one preschooler-mother dyads-involved with child welfare as documented perpetrators of child abuse or neglect, or non-maltreating (non-CM)-were observed completing a resting baseline and joint challenge task. Parenting behaviors were coded using SASB (Benjamin, 1996) and maternal RSA was simultaneously monitored, longitudinally-nested within-person (WP), and subjected to MLM. Abusive and neglectful mothers displayed less positive parenting and more strict/hostile control, relative to non-CM mothers. Non-CM mothers displayed greater WP heterogeneity in variance over time in their RSA scores, and greater consistency over time in their parenting behaviors, relative to abusive or neglectful mothers. CM group also moderated concurrent and lagged WP associations in RSA and positive parenting. When abusive mothers displayed lower RSA in a given epoch, relative to their task average, they showed concurrent increases in positive parenting, and higher subsequent levels of hostile control in the following epoch, suggesting that it is physiologically taxing for abusive mothers to parent in positive ways. In contrast, lagged effects for non-CM mothers were observed in which RSA decreases led to subsequent WP increases in positive parenting and decreases in control. Reversed models were significant only for neglectful mothers: Increases in positive parenting led to subsequent increases in RSA levels, and increases in strict, hostile control led to subsequent RSA decreases. These results provide new evidence that concurrent and time-ordered coupling in maternal physiology and behavior during parenting vary in theoretically meaningful ways across CM and non-CM mothers. Implications for intervention and study limitations are discussed.

The effects of cerebral white matter changes on cardiovascular responses to cognitive and physical activity in a stroke population.

Autonomic nervous system (ANS) control may be disrupted by cerebrovascular disease. We investigated the relationship between alterations in white matter integrity and regulation of the ANS in 23 participants who sustained a stroke within 5 years. These participants underwent diffusion tensor imaging, and fractional anisotropy values were calculated (DTI-FA) for each hemisphere and lobe. Cognitive and physical exertion tasks were performed while recording an electrocardiogram. Respiratory sinus arrhythmia (RSA) decreased more during a verbal fluency task with lower left hemisphere DTI-FA. Further, the physical stressor yielded decreases in RSA with lower frontal DTI-FA and higher temporal lobe DTI-FA, p < .05 (perhaps a release effect on the central autonomic network). Decrements in ANS regulation may have functional consequences that alter behavior, as well as potentially increasing the risk for further vascular disease.

Heart rate variability moderates the association between attachment avoidance and self-concept reorganization following marital separation

Despite substantial evidence indicating that relationships shape people's self-concept, relatively little is known about how people reorganize their sense of self when relationships end and whether this varies as a function of people's beliefs about relationships. In this report, we examine the prospective association between self-report adult attachment style and self-concept recovery among 89 adults following a recent marital separation. People high in attachment avoidance are characterized by the tendency to deactivate (i.e., suppress) painful attachment-related thoughts and feelings, and, following Fagundes, Diamond, and Allen (2012), we hypothesized that highly avoidant people would show better or worse self-concept outcomes depending on their ability to successfully regulate their emotional experience during a divorce-related mental recall task. We operationalized self-regulation using respiratory sinus arrhythmia (RSA) and found that highly avoidant people who showed RSA increases across our divorce-related mental activation task (DMAT) evidenced improvements in their self-concept over three months. In contrast, highly avoidant adults who showed RSA decreases during the DMAT showed no improvement (or a worsening) in their self-concept disruptions over the subsequent three months. These results suggest that RSA, an index of heart rate variability, may provide a window into self-regulation that has the potential to shed new light on why some people cope well or poorly following the loss of a relationship. Discussion centers on the potential mechanisms of action that explain why some people are able to successfully deactivate attachment-related thoughts and feelings whereas other people are not.

Love alters autonomic reactivity to emotions.

Periods of bond formation are accompanied by physiological and emotional changes, yet, little is known about the effects of falling in love on the individual's physiological response to emotions. We examined autonomic reactivity to the presentation of negative and positive films in 112 young adults, including 57 singles and 55 new lovers who began a romantic relationship 2.5 months prior to the experiment Autonomic reactivity was measured by Respiratory Sinus Arrhythmia (RSA) to two baseline emotionally neutral films, two negative films, and two positive films. Results demonstrated that RSA in singles decreased during the presentation of negative emotions, indicating physiological stress response. However, no such decrease was found among new lovers, pointing to more optimal vagal regulation during the period of falling in love. Autonomic reactivity, indexed by RSA decrease from the positive to the negative films, was greater among singles as compared to lovers, suggesting that love buffers against autonomic stress and facilitates emotion regulation. Findings suggest that vagal regulation may be one mechanism through which love and attachment reduce stress and promote well-being and health.

Incoherent oscillations of respiratory sinus arrhythmia during acute mental stress in humans

Respiratory sinus arrhythmia (RSA) has been widely used as a measure of the cardiac vagal control in response to stress. However, RSA seems not to be a generalized indicator because of its dependency on respiratory parameter and individual variations of RSA amplitude (A(RSA)). We hypothesized that phase-lag variations between RSA and respiration may serve as a normalized index of the degree of mental stress. Twenty healthy volunteers performed mental arithmetic task (ART) after 5 min of resting control followed by 5 min of recovery. Breathing pattern, beat-to-beat R-R intervals, and blood pressure (BP) were determined using inductance plethysmography, electrocardiography, and a Finapres device, respectively. The analytic signals of breathing and RSA were obtained by Hilbert transform and the degree of phase synchronization (λ) was quantified. With the use of spectral analysis, heart rate variability (HRV) was estimated for the low-frequency (LF) and high-frequency (HF) bands. A steady-state 3-min resting period (REST), the first 3 min (ART1), and the last 3 min (ART2) of the ART period (ranged from 6- to 19 min) and the last 3 min of the recovery period (RCV) were analyzed separately. Heart rate, systolic BP, and breathing frequency (f(R)) increased and λ, A(RSA), and HF power decreased from REST to ART (P < 0.01). The λ was correlated with normalized A(RSA) and the HF power. The decrease in λ could not be explained solely by the increase in f(R). We conclude that mental stress exerts an influence on RSA oscillations, inducing incoherent phase lag with respect to breathing, in addition to a decrease in RSA.

Autonomic regulation in fragile X syndrome

Autonomic reactivity was studied in individuals with fragile X syndrome (FXS), a genetic disorder partially characterized by abnormal social behavior. Relative to age-matched controls, the FXS group had faster baseline heart rate and lower amplitude respiratory sinus arrhythmia (RSA). In contrast to the typically developing controls, there was a decrease in RSA with age within the FXS group. Moreover, within the FXS group heart rate did not slow with age. The FXS group also responded with an atypical increase in RSA to the social challenge, while the control group reduced RSA. In a subset of the FXS group, the autonomic profile did not change following 2 months and 1 year of lithium treatment. The observed indices of atypical autonomic regulation, consistent with the Polyvagal Theory, may contribute to the deficits in social behavior and social communication observed in FXS.

Association between Cold Face Test‐induced vagal inhibition and cortisol response to acute stress

Low vagal function is related to several disorders. One possible underlying mechanism linking the vagus nerve and disorders is the HPA axis. Thirty-three healthy male subjects participated in a stress task, while heart rate (HR), respiratory sinus arrhythmia (RSA), salivary cortisol, and mood were assessed. Vagal function was determined using baseline, stress-induced inhibition, and Cold Face Test (CFT)-induced stimulation. The stress task induced a significant increase in cortisol and HR, a decrease in RSA, and a worsening of mood. A linear regression model with the time from CFT onset until maximum bradycardia as the independent variable explained 17.9% of the total variance in cortisol in response to the stressor (mood: 36.5%). The results indicate that a faster CFT response is associated with reduced cortisol increase and enhanced mood after acute stress. Our data support an inverse relationship between vagal function and the HPA axis.

Longitudinal Evidence for Unfavorable Effects of Antidepressants on Heart Rate Variability

It was previously shown that antidepressants are associated with diminished vagal control over the heart. Longitudinal studies are needed to test the causality of this association further. Longitudinal data were obtained in the Netherlands Study of Depression and Anxiety. At baseline and at 2-year follow-up, heart rate and cardiac vagal control as indexed by respiratory sinus arrhythmia were measured in 2114 subjects (mean age = 42.0 years; 66.2% female), who either used antidepressants at one or two time points (n = 603) or did not use antidepressants at any time point (n = 1511). Linear mixed-model analyses were conducted to compare changes in respiratory sinus arrhythmia and heart rate over time across antidepressant-naive subjects, subjects who started using an antidepressant during follow-up, subjects who stopped using an antidepressant, and persistent antidepressant users. Analyses were adjusted for demographics, health, and lifestyle factors. Compared with continuous nonusers, subjects who started the use of a tricyclic antidepressant or a serotonergic and noradrenergic antidepressant showed a significantly greater increase in heart rate and a decrease of respiratory sinus arrhythmia at 2 years. Subjects who started the use of selective serotonin reuptake inhibitors also showed a decrease in respiratory sinus arrhythmia, but their heart rate did not increase. Discontinuing antidepressants systematically caused opposite effects; levels returned in the direction of those observed among nonusers. These 2-year longitudinal results indicate that all antidepressants cause a decrease in cardiac vagal control. After discontinuing antidepressants, autonomic function recovers, suggesting that the unfavorable effects are (partly) reversible.

Decreased respiratory sinus arrhythmia in individuals with deceptive intent

In detecting deception, the Cognitive Load hypothesis states that lying requires more cognitive resources compared to truth telling. Further, increases in cognitive load are predicted to decrease respiratory sinus arrhythmia (RSA). We evaluated the impact of cognitive tasks and the intent to deceive on RSA in 40 male, native Arabic-speaking participants quasi-randomized into truthful (n=14) or deceptive (n=26) groups. Participants donned an ambulatory physiologic recording device and completed cognitive testing after receiving translated instructions about their role in an impending mock crime. The results show that a decrease in RSA recorded during the cognitive testing was greater in individuals who were about to commit a deceptive act.

Does respiratory sinus arrhythmia serve a buffering role for diastolic pressure fluctuations?

Though many consider the magnitude of respiratory sinus arrhythmia as an index of cardiac vagal control, its physiological origins remain unclear. One influential model postulates that the systolic pressure rise within a given beat stimulates the baroreflex arc to adjust the following heart period such that diastolic pressure is "stabilized" and hence displays lesser fluctuation. Accordingly, the magnitude of diastolic pressure fluctuations with respiration should change reciprocally after augmentation or inhibition of respiratory sinus arrhythmia. To test this, we augmented and subsequently inhibited respiratory sinus arrhythmia with vagotonic and vagolytic atropine administration in 19 healthy young volunteers to assess the relation between respiratory R-R interval and diastolic pressure fluctuations. Respiratory diastolic pressure fluctuations showed parallel rather than inverse changes in relation to those in respiratory sinus arrhythmia: they increased with augmented respiratory sinus arrhythmia (138 and 190% of baseline in the frequency and time domains, both P < 0.05) and tended to decrease with inhibited respiratory sinus arrhythmia (82 and 93% of baseline in frequency and time domains, P = 0.20 and P = 0.07). Furthermore, >60% of the change in diastolic pressure fluctuations was explained by the change in respiratory sinus arrhythmia (R(2) = 0.62; P < 0.001), that is, an approximately 50-ms increase or decrease in respiratory sinus arrhythmia resulted in a parallel approximately 1-mmHg change in diastolic pressure fluctuations. Thus, in young healthy individuals during supine rest, respiratory fluctuations in R-R interval do not buffer against diastolic pressure fluctuations but actually cause diastolic pressure fluctuations. Therefore, our data provide little evidence for a predominant role of a baroreflex feedback mechanism underlying respiratory sinus arrhythmia during supine rest.

Respiratory sinus arrhythmia reactivity predicts emotion regulation and depressive symptoms in at-risk and control children

We examined respiratory sinus arrhythmia (RSA), emotion regulation (ER), and prospective depressive symptoms in children at risk for depression and controls. Of the 65 children (35 boys; 5–13 years) in the sample, 39 had a parent with childhood-onset mood disorder and 26 had a parent with no history of major psychiatric disorder. RSA during pre- and post-film baselines and RSA reactivity to sad film clip were measured. Later, children's ER responses (focusing on sad/distressing affect) were assessed using a parent-reported questionnaire, and depressive symptoms were measured via clinical ratings. Results indicated that, compared to the initial baseline, a greater decrease in RSA (i.e., more vagal withdrawal) in response to the sad film clip predicted more adaptive ER responses and lower levels of clinician-rated depressive symptoms. However, tests for ER as a mediator of the association between RSA reactivity and depressive symptoms were precluded because maladaptive, but not adaptive, ER was associated with depressive symptoms. Overall, results suggest that cardiac vagal withdrawal (a greater decrease in RSA) in response to an emotional stimulus reflects more adaptive parasympathetic activity, which could facilitate children's ability to effectively manage their sadness and distress and predict lower risk of depressive symptoms over time.

Mother–Infant Vagal Regulation in the Face‐To‐Face Still‐Face Paradigm Is Moderated by Maternal Sensitivity

Parents' physiological regulation may support infants' regulation. Mothers (N= 152) and 6-month-old male and female infants were observed in normal and disrupted social interaction. Affect was coded at 1-s intervals and vagal tone measured as respiratory sinus arrhythmia (RSA). Maternal sensitivity was assessed in free play. Mothers and infants showed opposite patterns of RSA change. During disrupted interaction, mothers' RSA increased and infants' decreased, suggesting self-regulation of distress. During reunion, although the typical pattern was for infants to return to baseline levels, infants of sensitive mothers and sensitive mothers both showed a significant decrease in RSA from baseline. Mothers' and infants' physiological responses may be a function of mutual responsiveness.

Predicting cardiac vagal regulation in early childhood from maternal-child relationship quality during toddlerhood.

The aim of this study was to examine the influence of maternal-child relationship quality during toddlerhood on early childhood physiological regulation. A community sample of 447 children (215 males) was recruited at age 2 for participation in the study using the Child Behavior Checklist [Achenbach [1992] Manual for the child behavior checklist/2-3 & 1992 profile. Burlington, VT: University of VT Department of Psychiatry]. Mothers and children were observed across several interactions in the laboratory at age 2 and mothers completed the Parenting Stress Index [Abidin [1995] Manual for the parenting stress index. Odessa, FL: Psychological Assessment Resources]. Relationship quality was assessed using laboratory measures of hostility, positive guidance, and stress related to the quality of the relationship as reported by mothers. Cardiac vagal regulation at age 2 was assessed across six challenge tasks, three in which the child and mother worked together and three in which the child worked independently, and was indexed by the magnitude of vagal withdrawal (decrease in respiratory sinus arrhythmia, RSA) to challenge. Results indicated that children displayed greater cardiac vagal regulation and heart rate acceleration during collaborative tasks versus independent tasks. In addition, maternal-child relationship quality predicted the degree of vagal regulation in children at age 5, even after controlling for early and concurrent level of behavior problems as well as 2-year cardiac vagal regulation. Children with poorer quality relationships displayed significantly poorer vagal regulation and lower heart rate acceleration (p < .01). These findings are discussed in terms of the implications of environmental factors for the acquisition of fundamental self-regulatory skills.

Differential endophenotypic markers of narcissistic and antisocial personality features: A psychophysiological investigation

This study investigated the differential psychophysiological correlates of narcissistic and antisocial personality features in a college student sample. Skin conductance (SC), respiratory sinus arrhythmia (RSA), and pre-ejection period (PEP) were monitored while participants watched a countdown to an aversive noise blast and viewed emotionally valenced slides. Results indicated that narcissistic personality features were unrelated to SC reactivity during the countdown, whereas antisocial personality features were negatively related to SC reactivity. Narcissistic personality features were also related to RSA decreases and PEP shortening while viewing happy slides, whereas antisocial personality features were not. Taken together, these findings suggest differential endophenotypic markers of narcissistic and antisocial personality features despite their clinical similarities.

Does motor activity during psychophysiological paradigms confound the quantification and interpretation of heart rate and heart rate variability measures in young children?

Respiratory sinus arrhythmia (RSA), heart period, and motor activity were monitored in preschoolers during a variety of tasks varying in required movement. The data analyses indicate: (1) that when activity increases during tasks, there are synchronous decreases in heart period and RSA; (2) that correlations between changes in RSA and heart period are related to activity only during exercise when there is a major demand for increased metabolic resources; and (3) that the covariation among the variables within each condition is low except during exercise. These findings suggest that the slight increases in motor activity (i.e., hand movements) often required in attention demanding psychophysiological protocols are not related to RSA and heart period responses. However, when tasks necessitate large increases in motor activity (e.g., exercise), the decreases in heart period and RSA are related to the change in motor activity.

Infant Resilience to the Stress of the Still‐Face

Respiratory sinus arrhythmia (RSA) is related to infant emotion regulation and resilience. However, few studies have examined RSA of infants and mothers during a stressful experience. Even fewer studies have measured infant and mother skin conductance (SC), which in part reflects anxiety. This pilot study examined RSA, heart rate (HR), and SC patterns of 12 five-month-old infants and their mothers during normal interaction and a stressful perturbation of the interaction in which the mother does not respond to her infant-the Face-to-Face Still-Face (FFSF) paradigm. Dyads were grouped into four categories by two conditions: whether the infant protested to the Still-Face episode (SF) and whether they "recovered" from the SF by reducing protest when the mother resumed interaction in the Reunion (RE). Infants who recovered from the SF had the largest increase in RSA from SF to RE. Mothers of infants who recovered from the SF showed a decrease in RSA during the RE, suggesting mobilization of infant soothing behaviors. Mothers of infants who did not recover from the SF showed physiologic markers of anxiety in the form of continued increases in RSA and high levels of SC. Furthermore, these mothers behaved in a manner that was not responsive to their infant's disengagement cues. These pilot results demonstrate the feasibility of measuring infant SC, a measure long disregarded in infant research. The findings suggest that maternal psychophysiology may be related to infant resilience and suggest a bidirectional effect of maternal and infant reactivity.

Fentanyl induced decrease in respiratory sinus arrhythmia may be reversed by activation of 5‐HT4a receptors

Opiate exposure induces a centrally mediated respiratory inhibition, bradycardia, and a reduction in respiratory sinus arrhythmia (RSA). Previous work has shown that RSA is primarily mediated by an increase in both inhibitory GABAergic and glycinergic activity to cardiac vagal neurons (CVNs) during inspiration, and the mu-opioid receptor agonist, fentanyl inhibits both inspiratory neurons and GABAergic neurotransmission to CVNs. 5-HT4a receptor agonists have been shown to reverse fentanyl-induced respiratory depression. This study examines whether the 5-HT4a agonist, BIMU-8, reverses the fentanyl induced decrease in inspiratory-evoked GABA neurotransmission to CVNs. CVNs were identified by the presence of a retrograde fluorescent tracer and inspiratory evoked GABAergic synaptic currents were examined using whole-cell patch-clamp techniques in an in-vitro preparation that spontaneously generates rhythmic respiratory activity. Inspiratory activity was recorded by monitoring the motor neuron population activity from hypoglossal nerve roolets. BIMU-8 was administrated at a dose of 10microM alone or 10 minutes after fentanyl application. Application of BIMU-8 alone increased inspiratory burst frequency by 50%. Co-application of BIMU-8 with fentanyl prevented the opioid induced decrease in inspiratory-evoked GABAergic neurotransmission to cardiac parasympathetic neurons. This work demonstrates 5-HT4a specific receptor agonists overcome mu-opioid receptor induced respiration and RSA depression and may provide a potential therapeutic target for opiate treatment. Supported by NIH grants HL59895 and 72006 to DM.

Respiratory Sinus Arrhythmia During Stress Predicts Resting Respiratory Sinus Arrhythmia 3 Years Later in a Pediatric Sample.

The author examined whether respiratory sinus arrhythmia (RSA) responses to stress predicted resting RSA approximately 3 years later in children and adolescents. A total of 149 children and adolescents (49% girls and women, 44% African Americans) participated in 2 laboratory protocols approximately 3 years apart. RSA reactivity during tasks was consistent within participants across tasks during each session. Resting RSA at Visit 1 explained 17% of the variance in resting RSA at Visit 2 when body mass index, duration between visits, race, gender, and age were controlled for. Visit 1 RSA reactivity explained an additional 5% of the variance in resting RSA at Visit 2. The positive relationship between Visit 1 reactivity and Visit 2 resting levels suggests that larger decreases in RSA during stress predicted lower resting RSA. Conversely, increases in RSA during stress were associated with higher resting RSA an average of 3 years later.