Decreased N2O Emissions Research Articles

Freshwater Salinization Mitigated N2O Emissions in Submerged Plant-Covered Systems: Insights from Attached Biofilms.

Submerged plants (SMPs) play a critical role in improving water quality and reducing N2O greenhouse gas emissions. However, freshwater salinization represents a major environmental challenge in aquatic systems. To investigate the impact of salinization on N2O emissions, this study conducted indoor mesocosm experiments simulating SMP and nonsubmerged plant (Non_SMP) areas in freshwater lakes. The objective was to explore the effects and microbial mechanisms of the attached biofilm on N2O emission in freshwater salinization. Salinization systems (700-1500 μS cm-1) reduced N2O flux by 37.0 and 40.5% compared to freshwater systems (<700 μS cm-1) of SMPs and Non_SMPs, respectively. Kinetic experiments showed that the reduction in N2O emissions was mainly attributed to the attached biofilm rather than the sediment or water. The N2O net emission rates of the attached biofilm decreased by 47.1 and 71.8% in salinization systems of SMPs and Non_SMPs, respectively, compared with freshwater systems. Additionally, biofilms in salinization systems exhibited lower denitrification rates. Furthermore, salinization reduced the N2O production potential ((nirS + nirK)/(nosZI + nosZII)), thereby further decreasing N2O emissions. This study provides valuable insights into the role and mechanisms of biofilms in mitigating N2O emissions in salinized freshwater lakes.

Assessing biochar's impact on greenhouse gas emissions, microbial biomass, and enzyme activities in agricultural soils through meta-analysis and machine learning.

The role of biochar in reducing greenhouse gas (GHG) emissions and improving soil health is a topic of extensive research, yet its effects remain debated. Conflicting evidence exists regarding biochar's impact on soil microbial-mediated emissions with respect to different GHGs. This study systematically examines these divergent perspectives, aiming to investigate biochar's influence on GHG emissions and soil health in agricultural soils. The meta-analysis includes 2594 paired observations from 157 studies conducted between 2000 and 2024. It was found that biochar increased the presence of amoA and nosZ genes by 39.4% and 41.7%, respectively, while reducing the abundance of the nirS gene by 17.8%. This led to a 13.1% decrease in N2O emissions. Nitrous emissions were positively associated with mean annual temperature and biochar's pyrolysis temperature and dosage while inversely related to soil pH, nitrogen fertilisation rate, and biochar pH and carbon content. Biochar also regulated enzyme activity related to the nutrient cycle and increased microbial biomass carbon, nitrogen, and phosphorus by 16.6%, 23.9%, and 50.2%, respectively, leading to changes in microbial community diversity. These changes contributed to a reduction in CO2 and CH4 emissions, particularly when biochar and nitrogen fertiliser were applied at doses below 21.4tha-1 and 242.5kgha-1, as predicted by machine learning models. This study offers an overview of the positive impact of biochar amendments on soil GHG emissions mitigation. The key predictive factors identified could help optimise biochar production and targeted amendments, potentially improving soil health and achieving carbon neutrality in agroecosystems.

Effect of microplastics on soil greenhouse gas emissions: A global meta-analysis study.

Microplastics (MPs) emerged as a critical global pollutant, yet their effects on soil greenhouse gas (GHG) emissions remain uncertain. This meta-analysis evaluates the effects of MPs exposure on GHG emissions and identifies key influencing factors. Regardless of any influencing factors, MPs exposure decreased N2O emissions by 28.5%, while increased CO2 and CH4 emissions by 0.07% and 28.6%, respectively. However, these changes were statistically insignificant. The factors such as MPs concentration, shape, and type, initial soil type and pH, and the presence of additional additive were identified to significantly influence N2O emission response. The most substantial increase in N2O emissions occurred when MPs exposed to silt soils (+55.8%), whereas the greatest inhibition was observed in soils with fertilizer addition (-48.8%). CO2 emission response was significantly influenced by MPs size and shape, initial soil pH and type, experimental duration, and co-additives, with the MPs exposure in sand soils exhibiting the highest increase (+20.7%) and the exposure of fiber MPs causing the largest reduction (-40.4%). Additionally, MPs shape and initial soil pH and type were found to significantly affect CH4 emission response. The model selection analysis revealed that the response ratio [ln (RR)] of nirS gene to MPs exposure and MPs concentration were the most critical factors influencing N2O emissions, whereas the size, concentration, and type of MPs, ln (RR) of Chitinase, initial soil pH, additional additive, and experimental duration were the most important influencing factors for CO2 emissions. Overall, this study highlights the high uncertainty associated with the response of GHG emission to MPs exposure due to the complex interplay of abiotic and biotic processes mediated by MPs under varying conditions. In the future, extensive studies across diverse conditions of MPs (e.g., type, shape, and concentration) and soil (e.g., texture and pH) are still urgently needed.

Nutrient management offsets the effect of deoxygenation and warming on nitrous oxide emissions in a large US estuary.

Many estuaries experience eutrophication, deoxygenation and warming, with potential impacts on greenhouse gas emissions. However, the response of N2O production to these changes is poorly constrained. Here we applied nitrogen isotope tracer incubations to measure N2O production under experimentally manipulated changes in oxygen and temperature in the Chesapeake Bay-the largest estuary in the United States. N2O production more than doubled from nitrification and increased exponentially from denitrification when O2 was decreased from >20 to <5 micromolar. Raising temperature from 15° to 35°C increased N2O production 2- to 10-fold. Developing a biogeochemical model by incorporating these responses, N2O emissions from the Chesapeake Bay were estimated to decrease from 157 to 140 Mg N year-1 from 1986 to 2016 and further to 124 Mg N year-1 in 2050. Although deoxygenation and warming stimulate N2O production, the modeled decrease in N2O emissions, attributed to decreased nutrient inputs, indicates the importance of nutrient management in curbing greenhouse gas emissions, potentially mitigating climate change.

Co-application of glucose and phosphorus with recalcitrant high-carbon soil amendments improves N retention in a reclaimed soil: a long-term incubation study

ABSTRACT Incorporation of soil amendments with high organic carbon content (HCA) can reduce losses of mineral nitrogen (N) from agricultural soils. The magnitude of N immobilization and remobilization is strongly controlled by the availability of carbon (C) and phosphorus (P). However, the exact mechanisms and interactions between C, N, and P availability are poorly understood. An eight-month incubation experiment was conducted on recultivated mine soil with low organic C, mineral N and P background concentrations to investigate the effects of HCA in combination with 13C-labelled glucose and mineral P fertilization on greenhouse gas emissions, soil nutrient status (dissolved organic C (DOC), nitrate (NO3 –), extractable P), and microbial biomass growth. The experiment had a factorial design of one N level × two P levels × six C treatments (control, wheat straw, poplar sawdust, glucose, and combinations of wheat straw or sawdust with glucose). The HCA increased the cumulative CO2 and CH4 emissions but decreased N2O emission, except for wheat straw. Addition of 13C-labelled glucose decreased the cumulative CH4 emission by 59 and 85 % in the sawdust and sawdust + P treatment, respectively. Glucose application reduced the NO3 – content in the HCA-amended soil by 26–64 %, while P fertilizer further decreased the NO3 – content in the wheat straw and sawdust treatments by 20 and 24 %, respectively. Both HCA and glucose treatments promoted microbial biomass growth and reduced the soil mineral N content. The δ13C of microbial biomass (δ13CMB) showed an increasing trend during the whole experiment, although 13C-labelled glucose was added only once at the beginning of the experiment. Addition of HCA decreased δ13CMB, while P addition had the opposite effect. In conclusion, adding a readily available C source to HCA may increase the efficacy of retaining N in post-harvest soils, particularly of more recalcitrant types of HCA like sawdust.

Responses of N2O, CO2, and NH3 Emissions to Biochar and Nitrification Inhibitors Under a Delayed Nitrogen Application Regime

Greenhouse gas and NH3 emissions are exacerbated by the inappropriate timing and excessive application of nitrogen (N) fertilizers in wheat cultivation in China. In this study, the impacts on N2O, CO2, and NH3 emissions of a delayed and reduced N application regime on the Huang-Huai-Hai Plain were investigated. The treatments comprised the control (N0), conventional N at 270 kg N ha−1 (N270) and optimized N application of 180 kg N ha−1 (N180), N180 + biochar at 7.5 t ha−1 (N180B7.5), N180 + biochar at 15 t ha−1 (N180B15), N180 + DMPP (a nitrification inhibitor; N180D), N180D + biochar at 7.5 t ha−1 (N180DB7.5), and N180D + biochar at 15 t ha−1 (N180DB15). Reduced N application (N180) lowered N2O and NH3 emissions. Biochar application resulted in a 4–25% and 12–16% increase in N2O and NH3 emissions, respectively. Application of DMPP significantly decreased N2O emissions by 32% while concurrently inducing a 9% increase in NH3 emissions. Co-application of DMPP and biochar significantly reduced the activity of nitrification enzymes (HAD, NOO), resulting in a reduction of 37–38% in N2O emissions and 13–14% in NH3 emissions. No significant differences in CO2 emissions were observed among the various N treatments except the N0 treatment. Application of DMPP alone did not significantly affect grain yield. However, biochar, in combination with DMPP, effectively increases grain yield. The findings suggest that the N180DB15 treatment has the potential to reduce emissions of N2O and NH3 while concurrently enhancing soil fertility (pH, SOC) and wheat yield.

Modifying functional groups of straw-based hydrogel provide soil N2O mitigation potential by complete denitrification

Farmed soil is considered to contribute more than 60% anthropogenic N2O emission which plays critical role in global warming potential. Straw-based hydrogels with abundant functional groups are commonly used in environmental and agronomic applications primarily as adsorbents. Those functional groups could mediate electron transfer in the process of adsorbates and might also regulate N2O emission. However, it was still unclear whether and how these functional groups affect the process. To address this knowledge gap, we employed spectral analysis and a robotized incubation system to determine mechanisms of different functional groups in influencing soil N2O. The results indicated that the straw-based hydrogel was capable of significantly reducing the emission of N2O in denitrification, and the electrochemical properties of the hydrogel had promoting effect of the microbial genetic potential for N2O reduction. Specifically, carboxymethyl (R-COO) and primary amine groups (R-NH2) promoted complete denitrification through electron supply. Furthermore, R-NH2 had a significant negative correlation with N2O emissions. To further verify the role of specific structures and functional groups in denitrification, we conducted an experiment using pure lignin as a template. This experiment resulted in an increase in R-NH2 content to 13.2% and a decrease in N2O emissions by 49.1% compared with straw hydrogel. These results prove the feasibility of straw-based hydrogel as a redox system to accelerate complete denitrification, and the electron-donating functional groups were significantly related to the reduction rate of N2O. Finally, based on these mechanisms, functional group targeted grafting technology was proposed to improve its ability to mitigate N2O emission reduction.

Key biochar properties linked to denitrification products in a calcareous soil

Meta-analyses show an overall decrease in soil N2O emissions after biochar (BC) amendment. Nonetheless, N2O mitigation with BC cannot be extrapolated to every BC-soil combination, inasmuch as an increase in soil N2O release has been occasionally reported. We hypothesized that BC characteristics are key, and performed two microcosm experiments to advance in the understanding of the properties associated. We first investigated how 22 well-characterized BCs affect N2O emissions in a calcareous soil under denitrification conditions. Whereas most BCs decreased N2O emissions, some substantially increased N2O emissions. In a second experiment, we selected and further characterized eight of the 22 previous BCs. We applied the 15N-gas-flux method to study how these BCs affect denitrification products (N2O and N2) in the same soil. Results indicate that the interaction between BC and the denitrification process depends on the temperature of pyrolysis. Whereas BCs produced at 400 °C tended to increase total denitrification (N2O+N2) by an average of 28%, BCs produced at 600 °C significantly reduced total denitrification by 53%. Nevertheless, this decline in overall denitrification did not result in a decrease of N2O emissions, as there was a strong shift in the N2O/(N2+N2O) ratio favoring N2O. A redundancy analysis revealed a direct correlation between carboxylic groups on BCs surface and N2O emissions. This research enhances our understanding of the interaction of BC with denitrification, particularly concerning the relevance of the temperature of pyrolysis, and opens up new paths for investigation, crucial for optimizing the application of BCs in different soil environments.Graphical

Rapid Responses of Greenhouse Gas Emissions and Microbial Communities to Carbon and Nitrogen Addition in Sediments.

Massive labile carbon and nitrogen inputs into lakes change greenhouse gas emissions. However, the rapid driving mechanism from eutrophic and swampy lakes is not fully understood and is usually contradictory. Thus, we launched a short-term and anaerobic incubation experiment to explore the response of greenhouse gas emissions and microbial communities to glucose and nitrate nitrogen (NO3--N) inputs. Glucose addition significantly increased CH4 and CO2 emissions and decreased N2O emissions, but there were no significant differences. NO3--N addition significantly promoted N2O emissions but reduced CH4 accumulative amounts, similar to the results of the Tax4Fun prediction. Bacterial relative abundance changed after glucose addition and coupled with the abundance of denitrification genes (nirS and nirK) decreased while maintaining a negative impact on N2O emissions, considerably increasing methanogenic bacteria (mcrA1) while maintaining a positive impact on CH4 emissions. Structural equation modeling showed that glucose and NO3--N addition directly affected MBC content and greenhouse gas emissions. Further, MBC content was significantly negative with nirS and nirK, and positive with mcrA1. These results significantly deepen the current understanding of the relationships between labial carbon, nitrogen, and greenhouse emissions, further highlighting that labile carbon input is the primary factor driving greenhouse gas emissions from eutrophic shallow lakes.

Long‐term tillage and cover cropping differentially influenced soil nitrous oxide emissions from cotton cropping system

AbstractClimate‐smart agricultural practices, such as no‐tillage (NT) and cover cropping, have been widely adopted and are anticipated to yield multiple benefits, including soil carbon sequestration, enhancing soil health, and crop yield stability. However, their influence on nitrous oxide (N2O) emissions varies, with the potential of both increasing and decreasing N2O emissions. Increasing N2O emissions under these practices may potentially offset the climate mitigation benefits from increased soil carbon sequestration. We investigated N2O emissions in response to 42 years of long‐term adoption of NT and legume cover crop, under nitrogen (N) rates of 0 and 67 kg N ha−1, in a continuous cotton system in the Southeastern United States. Intensive manual chamber‐based measurements were conducted over two growing seasons (2021–2022 and 2022–2023). Long‐term NT did not significantly affect cumulative N2O emissions during the study period (p &gt; 0.05). Hairy vetch cover crop‐grown plots emitted two to three times more N2O than those without cover crops in 2021–2022, with no significant effect observed in 2022–2023. Cumulative emissions in cover crop plots were greater compared to those in no cover crop plots when fertilized with 67 kg N ha−1 in 2021–2022; however, this trend did not persist in 2022–2023. While interannual variability exists, our results generally suggest that long‐term NT may not increase N2O emissions, hence its adoption could enhance its broader soil health and climate benefits via soil carbon sequestration, whereas managing legume cover crop residues in N‐fertilized systems is critical to mitigate N2O emissions.

Numerical investigation and optimization of the ammonia/diesel dual fuel engine combustion under high ammonia substitution ratio

This study investigated the effects of initial temperature, equivalence ratio, and diesel injection timing on engine combustion and emission characteristics at high ammonia substitution ratios. Increased compression temperature and pressure significantly reduce ignition delay, enhance combustion speed and efficiency, and decrease N2O and unburned NH3 emissions. A strong correlation exists between the amount of N2O produced and the mass of unburned NH3 when ammonia combustion efficiency is high. The N2O distribution is concentrated near the cylinder walls and the piston top surface, in areas with high concentrations of unburned NH3. As the equivalence ratio increases from 0.6 to 0.75, flame propagation speed and indicated thermal efficiency (ITE) increase, while NOx, N2O, and unburned NH3 emissions decrease. The combustion performance and emissions were optimized by advancing the diesel injection timing and increasing the equivalence ratio to accelerate the combustion speed. This adjustment increases ITE to 47.6 % at an 80 % ammonia energy ratio. Post-optimization results show a reduction in unburned NH3 emissions from 51.7 g/kW·h to 5.9 g/kW·h and a decrease in N2O emissions from 0.930 g/kW·h to 0.370 g/kW·h, culminating in a 60.4 % reduction in greenhouse gas (GHG) emissions.

Converse Responses of Biochar Application on N2O Emissions in Soils at Different pH Values in a Subtropical Citrus Orchard

The aim of this study was to explore the effect of biochar on N2O emissions in soils with different pH levels. Soils with five pH levels (4.0, 5.1, 5.8, 6.6, and 7.2) were incubated in two conditions, with 0% biochar (CK) and 1% biochar (BC), for 23 days. N2O emissions were measured at nine time points, and soil chemical properties, AOA-amoA, AOB-amoA, nirK, nirS, and nosZ, were analyzed. Partial least squares path modelling (PLS-PM) was used to assess the effect of nitrification and denitrification pathways on potential N2O emissions. The results showed that biochar reduced N2O emissions in highly acidic soil (pH 4.0) but increased emissions in soils with pH values ranging from 5.1 to 7.2. In highly acidic soils, decreased N2O emission was associated with increased soil pH (p &lt; 0.05) and decreased dissolved organic carbon content (p &lt; 0.05), leading to higher nosZ gene abundance (p &lt; 0.05). Meanwhile, in acidic to neutral soils, biochar application increased soil pH (6.6–11.7%), dissolved organic nitrogen (5.9–29.5%), dissolved organic carbon (8.6–41.0%), stimulated AOB-amoA, nirK, nirS gene abundance (p &lt; 0.05), and thus increased N2O emissions. The results verified the influence of nitrification and denitrification genes on N2O production in soils with different pH values. In conclusion, biochar had different effects on N2O emissions based on soil pH, highlighting the need to consider pH when using biochar to mitigate N2O emissions in subtropical citrus orchards.

Low nitrous oxide fluxes from mineral affected peatland soils in Iceland

Nitrous oxide (N2O) is a potent greenhouse gas, and soil is one of its most substantial sources. Emissions are influenced by both biotic and abiotic processes, as well as the physiochemical properties of the medium. Organic soils fully drained in boreal climates are a potentially significant source of nitrous oxide. Lower, drainage levels and nutrient availability have been shown to reduce N2O emissions. Predicting N2O emissions from agriculturally managed peatlands is challenging. In situ measurements are crucial for addressing these difficulties. This study presents three years of in situ field measurements of nitrous oxide fluxes and soil hydrological status in peatland soils under different agricultural management regimes: cultivated drained (CD), uncultivated drained (UCD), and wet (W) in Western Iceland. Fluxes were quantified by analysing a time-concentration series obtained from permanent chambers and subsequently evaluated using gas chromatography in the laboratory. The minimum potential N2O consumption rate (mean ± 95 % Cl) was estimated as 0.76 ± 0.38, 0.56 ± 0.18 and 0.56 ± 0.14 mg N2O-N m⁻² day⁻¹ for CD, UCD, and W sites, respectively. Yearly accumulated emissions were estimated as 2.24 ± 1.47, 1.26 ± 0.64 and 0.26 ± 0.44 kg N2O-N ha−1 yr−1 for CD, UCD, and W sites, respectively. The relatively low emission rates observed at drained sites can be explained by the stable water content, and by limited availability of soil phosphorus, which hampers N2O production and potentially leads to more active N2O consumption. Both the stable water content and low phosphorus availability is explained by mineral inputs to the peatlands, acting through decreased soil unsaturated hydraulic conductivity and increased phosphorus retention. The effects of mineral content offer potential mitigation option to decrease N2O emissions. This study highlights the complexity of N2O emissions from peatland soils and emphasizes the significance of site-specific factors in managing greenhouse gas emissions. Further studies investigating the underlying processes behind N2O fluxes in the peatland and andic soils are recommended.

The influence of exhaust gas recirculation on combustion and emission characteristics of ammonia-diesel dual-fuel engines: Heat capacity, dilution and chemical effects

As the greenhouse effect intensifies, ammonia is garnering increasing attention as a carbon-free fuel. In the transport sector, ammonia-diesel dual-fuel (ADDF) engines are regarded as an effective means of reducing carbon emissions. The objective of this study is to investigate the combustion and emission optimization of an ADDF engine under high load conditions. To this end, an experimental optimization study of different start of diesel injection timing (SODI) and exhaust gas recirculation (EGR) rates was conducted at a load of 18 bar and an ammonia energy ratio of 80 %. The mechanism of heat capacity, dilution, and chemical effects of EGR was also revealed by numerical simulation based on the separated variables method. It was demonstrated that advancing SODI is effective in enhancing combustion efficiency. However, this approach is limited by the upper limit of in-cylinder pressure and results in higher nitrogen oxides (NOx) emissions, which can be mitigated by the EGR. The heat capacity effect of EGR increases the specific heat capacity and decreases the average temperature. The suppression of the combustion process leads to a reduction in thermal and fuel NOx, but an increase in nitrous oxide (N2O) emissions. The dilution effect of EGR results in insufficient oxygen, which decreases the heat release rate and combustion efficiency. Additionally, the NOx and N2O are significantly reduced. The chemical effect of EGR affects reactive groups and unburned components that accelerate heat release rate and increase accumulated heat release, resulting in significantly higher NOx. The comprehensive effect of EGR results in a decrease in N2O emissions and a significant reduction in thermal and fuel NOx. The EGR and further optimization of SODI enabled the ADDF engine to achieve a gross indicated thermal efficiency of 48.5 % with a load of 18 bar and an ammonia energy ratio of 80 %. In addition, NO emissions were reduced by 32.8 percent and greenhouse gas emissions by 63.3 percent.

Rice-crayfish integrated system enhances global warming potential via increasing methane emission mainly driven by continuous deep flooding

The conversion from rice monoculture (RM) to rice-crayfish integrated system (RCIS) could change soil physicochemical properties and microorganisms related to greenhouse gas (GHG) emissions. Nevertheless, it is still unclear the responses of GHG emissions and global warming potential (GWP) from paddy fields to RCIS. Here, we conducted a field experiment to investigate the changes in the emissions of methane (CH4) and nitrous oxide (N2O), GWP, soil physicochemical properties and the associated microbial abundances (methanogen, methanotrophs, denitrifier and nitrifier) under RCIS in Gaoyou City, Jiangsu Province, China from 2022 to 2023. There were three treatments, including RM, rice monoculture with continuous deep flooding (RMF) and RCIS. Compared with RM, RCIS and RMF all significantly increased CH4 emission and decreased N2O emission. But the effectiveness of increasing CH4 emission and decreasing N2O emission was larger under RCIS than RMF. The increased CH4 emission was due to the significantly higher mcrA gene abundance and ratio of mcrA/pmoA under RCIS. While the significantly higher nosZ gene abundance and ratio of nosZ/(amoA+nirK+nirS) under RCIS were responsible for its reduced N2O emission. Furthermore, the increases in gene abundances of mcrA and nosZ under RCIS were closely correlated with the significantly lower redox potential and pH as well as the significantly higher contents of dissolved organic carbon, ammonium and nitrate in soil. Averaged across two years, GWP under RCIS and RMF were 4.6-fold and 3.4-fold that under RM, respectively. This revealed that continuous deep flooding had a greater effectiveness in increasing GWP than crayfish culture under RCIS. Notably, the contribution rates of CH4 emission to the total GWP decreased in the order of RCIS (98.6%) > RMF (97.5%) > RM (86.2%). Besides, greenhouse gas intensity under RCIS was 5.1-fold that of RM due to its enhanced GWP and reduced rice grain yield. In summary, RCIS could enhance GWP from paddy fields through increasing CH4 emission mainly caused by the continuous deep flooding.

Effects of biodegradable microplastics and straw addition on soil greenhouse gas emissions

Large pieces of plastic are transformed into microplastic particles through weathering, abrasion, and ultraviolet radiation, significantly impacting the soil ecosystem. However, studies on biodegradable microplastics replacing traditional microplastics as agricultural mulching films to drive the biogeochemical processes influenced by GHG are still in their initial stages, with limited relevant reports available. This study sought to investigate the effects of microplastic and straw addition on CO2 and N2O emissions in different soils. Herein, yellow-brown soil (S1) and fluvo-aquic soil (S2) were utilized, each treated with three different concentrations of PLA (polylactic acid) microplastics (0.25%, 2%, and 7% w/w) at 25 °C for 35 days, with and without straw addition. The results showed that straw (1% w/w) significantly increased soil CO2 by 4.1-fold and 3.2-fold, respectively, and N2O by 1.8-fold and 1.8-fold, respectively, in cumulative emissions in S1 and S2 compared with the control. PLA microplastics significantly increased CO2 emissions by 71.5% and 99.0% and decreased N2O emissions by 30.1% and 24.7% at a high concentration (7% w/w, PLA3) in S1 and S2 compared with the control, respectively. The same trend was observed with the addition of straw and microplastics together. Structural equation modeling and redundancy analysis confirmed that soil physiochemical parameters, enzyme and microbial activities are key factors regulating CO2 and N2O emissions. The addition of microplastics is equivalent to the addition of carbon sources, which can significantly affect DOC, MBC, SOC and the abundance of carbon-associated bacteria (CbbL), thereby increasing soil CO2 emissions. The addition of microplastics alone inhibited the activity of nitrogen cycling enzymes (urease activity), increasing the abundance of denitrifying microbes. However, adding a high amount of microplastics and straw together released plastic additives, inhibiting microbial abundance and reducing the nitrogen cycle. These effects decreased NH4+-N and increased NO3−-N, resulting in decreased N2O emissions. This study indicates that biodegradable microplastics could reduce soil plastic residue pollution through degradation. However, their use could also increase CO2 emissions and decrease N2O emissions. Consequently, this research lays the groundwork for further investigation into the implications of utilizing biodegradable microplastics as agricultural mulch, particularly concerning soil geochemistry and GHG emissions.

Nanoparticles of Zinc Oxides Mitigated N2O Emissions in Tea Plantation Soil

The excessive application of nitrogen in tea plantations leads to severe soil acidification and N2O emission boosting. To promote sustainable agriculture, nanoparticles (NPs) have emerged as alternative fertilizers, but their effects on soil nitrification and greenhouse gas emissions in tea plantations remain unclear. In this study, the effects of NP type (ZnO-NPs and Fe2O3-NPs) and dose (0, 1, 10, and 100 mg·kg−1) on soil N2O emissions were investigated via a lab incubation trial. Soil pH, ammonium, and nitrate changes were also monitored during the incubation period. The abundance of functional genes related to nitrification and denitrification processes was analyzed as well. The results showed that ZnO-NPs led to a decrease in N2O emissions. The reduction effect was stronger with increasing dose and resulted in a 33% reduction at an addition rate of 100 mg·kg−1. The cumulative N2O emissions had significantly positive correlations with NH4+-N and NO3−-N. ZnO-NP addition showed a significantly negative effect on Ammonia-Oxidizing Archaea (AOA) but a positive effect on Ammonia-Oxidizing Bacteria (AOB) gene abundance. In contrast, Fe2O3-NPs showed an insignificant impact on N2O emissions and soil N content, as well as nitrification–denitrification gene abundance, regardless of different doses. These results imply that the application of ZnO-NPs may inhibit nitrification through the retarding of AOA activity. This study provided us with a potential practice to reduce N2O emissions in tea plantations by applying ZnO-NPs, but the efficiency of this reduction needs further examination under ambient conditions before field application.

NosZ I carrying microorganisms determine N2O emissions from the subtropical paddy field under elevated CO2 and strongly CO2-responsive cultivar

Elevated CO2 (eCO2) decreases N2O emissions from subtropical paddy fields, but the underlying mechanisms remain to be investigated. Herein, the response of key microbial nitrogen cycling genes to eCO2 (ambient air +200 μmol CO2 mol−1) in four rice cultivars, including two weakly CO2-responsive (W27, H5) and two strongly CO2-responsive cultivars (Y1540, L1988), was investigated. Except for nosZ I, eCO2 did not significantly alter the abundance of the other genes. NosZ I was a crucial factor governing N2O emissions, especially under eCO2 and a strongly responsive cultivar. eCO2 affected the nosZ I gene abundance (p < 0.05), for instance, the nosZ I gene abundance of cultivar W27 increased from 1.53 × 107 to 2.86 × 107 copies g−1 dw soil (p < 0.05). In the nosZ I microbial community, the known taxa were mainly Pseudomonadota (phylum) (19.74–31.72 %) and Alphaproteobacteria (class) (0.56–13.12 %). In the nosZ I community assembly process, eCO2 enhanced the role of stochasticity, increasing from 35 % to 85 % (p < 0.05), thereby inducing diffusion limitations of weakly responsive cultivars to dominate (67 %). Taken together, the increase in nosZ I gene abundance is a potential reason for the alleviation of N2O emissions from subtropical paddy fields under eCO2.

Wheat straw and microbial inoculants have an additive effect on N2O emissions by changing microbial functional groups

AbstractStraw‐decomposing microbial inoculants (MIs) have been increasingly applied to straw‐amended soils. However, the interactive effects and underlying microbial mechanisms of straw and decomposing MIs on nitrous oxide (N2O) emissions remain unclear. Here, a pot experiment with an Aquic Inceptisol was conducted to determine soil N2O emissions and the abundance and composition of microbial functional genes under six amendments: wheat straw (W), Bacillus subtilis MI (B), Streptomyces rochei MI (S), a combination of straw and B. subtilis MI (WB), a combination of straw and S. rochei MI (WS), and a control without wheat straw or MI (C). Compared with the control, the amendments of straw, decomposing MIs, and their combinations decreased soil N2O emissions by 43%, 22–30%, and 46–60%, respectively. Mechanistically, the positive relationship between ammonia‐oxidizing bacteria (AOB) and soil potential nitrification rate (PNR), along with decreased AOB abundance (−36%) following straw and decomposing MI amendments, further suggested that AOB predominated soil nitrification and was responsible for the suppressed nitrification. In addition, straw amendment increased nirK gene abundance (by 48%) and potential denitrification rate (by 11%), while the increase in nosZI gene abundance (+25%) involved in N2O consumption contributed to the decrease in N2O emissions. Overall, the additive effect of straw and decomposing MIs on soil N2O emissions was associated with two amendment‐induced changes in the abundance and composition of AOB and straw‐stimulated the abundance of the nosZI gene. Our results revealed the potential for mitigating N2O emissions following the amendments of straw and MI by influencing soil N2O‐related microbial groups.

Partial substitution of manure increases N2O emissions in the alkaline soil but not acidic soils

The fertilization regimes of combining manure with synthetic fertilizer are benefits for crop yields and soil fertility in cropping systems as compared to sole synthetic fertilization, but the responses of nitrous oxide (N2O) emissions to these practices are inconsistent in the literatures. We hypothesized that it is caused by different proportions of nitrogen (N) applied as manure and various soil properties. Here, we conducted a microcosm experiment, and measured the N2O emissions from control (no N) and five manure substitution treatments (supplied 100 mg N kg−1 using the combination of urea with manure) with a range of proportions of N applied as manure (0, 25%, 50%, 75%, and 100%) in three different soil types (fluvo-aquic soil, black soil, and latosol) under aerobic condition. The stimulated effect on N2O emissions was more pronounced after manure application in an alkaline soil with high nitrification rate, due to relatively rapid soil DOC depletion and N mineralization of manure. N2O emissions from partial substitution of urea with manure were significantly higher than manure-only addition under high soil pH due to abundant labile C from manure. However, there was no difference between manure substitution treatments under acid soils. Nitrification inhibitor substantially decreased N2O emissions with increasing soil pH, but it was less effective in mitigating N2O emissions with larger proportion of manure. This is likely due to the slow nitrification under low soil pH, and denitrification derived N2O increased with increasing manure application rate. Collectively, our study shows that the application of manure substitution to alkaline soils requires careful consideration, which might have rapid nitrification potential and hence trigger significant N2O emissions. The knowledge gained in this work will help the decision-makers in optimizing a sound N fertilization regime interacted with soil properties for sustainable crop production and N2O mitigation.