Bioclogging extensively exists in porous media, such as permeable reactive barrier, constructed wetland, reverse osmosis, and biofilter systems. Microorganisms overproduce and affect the efficiency of sewage treatment. In this paper, variations in biochemical and hydraulic parameters during the clogging process were obtained using various column experiments. The hydraulic conductivity first decreased sharply to 18.32 % of the original value at the 12th day and decreased to 2.71 % at the end of the experiment, a reduction of more than an order of magnitude. The hydrodynamic dispersion had the highest increase at 7.13 times the initial value and ultimately decreased to 29 %. The porosity decreased to 47.24 % of the initial value, and the total bacterial count in the inlet of the column increased from 3.4 × 106 to 8.8 × 108 cells/mL. Based on the biochemical and hydraulic parameter variation, the clogging process can be divided into four stages: (1) severe clogging occurs, and aerobic microorganisms reproduce rapidly in the inlet; (2) clogging exists in the entire column, and hydrodynamic dispersion increases sharply as aerobic and anaerobic microorganisms reproduce; (3) anaerobic microorganisms reproduce rapidly and produce more gas, and hydrodynamic dispersion decreases quickly; (4) aerobic and anaerobic microorganisms multiply continuously, and hydrodynamic dispersion, hydraulic conductivity, and porosity decrease steadily. Bioclogging then transforms into a steady stage.