Heparin is a highly sulfated polysaccharide used extensively as an anticoagulant. However, conventional heparin extraction processes remains time-consuming and inefficient. We report cationic brush magnetic adsorbents by grafting poly(3-acrylamidopropyl) trimethylammonium chloride-co-N‑isopropylacrylamide random copolymers onto the surface of Fe3O4 for ultrafast and efficient heparin extraction. The fabricated adsorbent demonstrated an excellent adsorption capacity of 169.36 mg g−1 and a record-breaking adsorption rate towards heparin due to the introduction of N‑isopropylacrylamide, providing more adsorption sites and larger transport channels for the macromolecule heparin to access active sites. In addition, the adsorption capacity and rate for crude heparin were significantly better than those of commercial resin. Thermodynamic parameters implied that the heparin adsorption was spontaneous and endothermic process. The adsorption mechanism can be described by the synergistic effect of electrostatic attraction and hydrogen bond. This study paved an innovative approach to simultaneously enhance the adsorption rate and capacity of the adsorbent for polysaccharide.