The western honey bee, Apis mellifera L. is an essential pollinator for high yield in agriculture and provides products of economic values. Recently, the sudden decline of the bee population by numerous causes has occurred, which is called colony collapse disorder (CCD). One of the reasons in CCD, the limited nutrition in colonies declines brood rearing and shortens the lifespan of adult workers. Beekeepers need to provide artificial bee feed regularly to maintain health colony and continuity of bee-related products on apiculture. This study focuses on development of artificial bee feed, through investigation of different contents in nutritional components on five samples, namely canola pollen, mixed pollen, bee bread, MegaBee, and Test A. Among them, Test A was developed as artificial bee feed and was compared with other samples. The five samples were analyzed on free sugar, organic acid and amino acid. Sucrose content showed the largest amounts with 46.15% and 59.60% on Megabee and Test A, respectively. The Test A showed the largest account for citric acid with 92.33%. A total of 21 amino acids were detected on five samples. Among the detected 21 amino acids, proline was accounted the highest content in canola pollen, mixed pollen and bee bread with 1159.21 mg/L, 998.90 mg/L and 783.73 mg/L, respectively. Among the five samples, Test A showed the highest amount of lysine with 471.30 mg/L. Nutritional content, balance and efficiency needs to be considered for the development of artificial bee feed. This study will contribute to provide future directions on development of artificial bee feed.