BackgroundProgressive tendon loading programs for patellar tendinopathy typically include single-leg squats with heavy weights either on level ground or on a decline board. Changes in patellar tendon force due to variations of the heavy load single-leg squat have not yet been objectively quantified. The objective of this study was to investigate the influence of the mass of an external weight and the use of a decline board on the peak patellar tendon force during a heavy load single-leg squat. MethodsTwelve healthy participants performed single-leg back squats on a decline board and level ground at 70%, 80% and 90% of their one repetition maximum. Three-dimensional kinematics and ground reaction forces were measured and the peak patellar tendon force was calculated using musculoskeletal modelling. A two-way repeated measures ANOVA determined the main effects for the mass of the external weights and the use of a decline board as well as their interaction effect. FindingsPeak patellar tendon forces were significantly higher on the decline board compared to level ground (p < 0.05). Neither on the decline board, nor on level ground did the peak patellar tendon force increase significantly when increasing the external weights (p > 0.05). InterpretationProgression in peak patellar tendon forces during a heavy load single-leg squat can only be obtained with a decline board. Increasing the mass of the external weight from 70% to 90% of the one repetition maximum does not result in a progressively higher peak patellar tendon force.