Deciduous Research Articles

Improving airborne laser scanning-based species-specific forest volume estimation using sentinel-2 time series

ABSTRACT Species-specific timber volume estimates are required to support forest planning and conservation. We evaluated whether additional predictors from a Sentinel-2 time series can improve airborne laser scanning (ALS)-based estimation of species-specific timber volumes. Furthermore, we determined the satellite image dates that provided the greatest improvement in accuracy. The Sentinel-2 time series was constructed to cover 1 March–30 November time-period, with a focus on late spring and early summer. The estimation was done using the k Most Similar Neighbor method and predictors extracted from the ALS data and Sentinel-2 images. Our best model included both ALS and Sentinel-2 time-series predictors, and the relative root mean square error (RMSE) values for pine, spruce and deciduous timber volumes were 40.8%, 57.0% and 51.3%, respectively (mean 49.7%). All deciduous trees were treated as one species. When bands from an individual image were used instead of the time series, the best result was obtained with an image from September where the respective relative RMSE values were 42.2% (deciduous), 58.4% (pine) and 60.6% (spruce) with a mean value of 53.7%. A fusion of a Sentinel-2 time-series and ALS data can improve species-specific estimation results compared to the use of individual Sentinel-2 images or ALS only.

Local environment perceived in daily life and urban green and blue space visits: Uncovering key factors for different age groups to access ecosystem services

Inequality in access to green and blue spaces is a major issue in the field of environmental justice. Various factors significantly influence visitations to these spaces, including residential, commuting, and shopping areas. However, studies have mainly focused on residential environments. Analyzing the coupling of environmental and socioeconomic factors, including age, income, and childhood experience, and examining the direct and indirect effects of these factors for different age groups are needed to better understand the detailed context of inequalities in access to ecosystem services. We conducted a questionnaire survey and analysis to address these needs in an urban setting. The results demonstrate that several land use categories (i.e., agricultural lands in residential areas, grasslands near shopping areas, and deciduous forests near commuting destinations) positively correlated with visitation frequency, suggesting the lack thereof may be the cause of access inequalities. In particular, the perimeter per unit area of some land cover types, which shows the complexity of the form of each land cover category, was found to be a significant factor. We identified inequality in nature experience to be the key factor correlated with inequality in nature visits among the young age group. For the middle-aged and old age groups, nature-relatedness was positively correlated with visitation frequency. Proper design of the local environment, such as through land use planning, can be an effective measure for all age groups. For instance, long-bordered green areas in the Monsoon Asian region are an attractive traditional landscape, with high accessibility and a comfortable thermal environment. The key factors for each age group should be considered in spatial design and inclusive information-sharing to reduce inequality in access to ecosystem services.

Regional Variability of Chestnut (Castanea sativa) Tolerance Toward Blight Disease

Since its introduction into Europe in the first half of the 20th century, Cryphonectria parasitica has been gradually spreading across the natural range of the sweet chestnut (Castanea sativa Mill.), infecting the trees and causing lethal bark cankers. Serendipitously, a hyperparasitic Cryphonectria hypovirus 1 (CHV1), which attenuates C. parasitica virulence in combination with more tolerant European chestnut species, was able to ward off the worst effect of the disease. In North America, unfortunately, the native Castanea dentata is now functionally extinct since it occurs only as root sprouts in eastern deciduous forests where it was once dominant. In our work, we investigated changes in C. parasitica populations over time and the regional variability in chestnut populations’ tolerance toward the blight disease. While vegetative compatibility (vc) type diversity and prevalence of hypovirulence remained similar as in previous studies, in the Buje population, unlike in previous studies, we were unable to find any hypovirulent fungal strains. The most common vegetative compatibility types (vc types) were EU-1, EU-2 and EU-12. However, several rare EU-types were found, including one previously unreported: EU-46. By inoculating several C. parasitica strains on tree stems from several chestnut populations, we observed that the induced lesion size was affected by the type of inoculum (CHV1-free or CHV1-infected), genotype-related individual chestnut stem and chestnut stem population of origin-related variability. The largest lesions were induced by CHV1-free fungal isolate DOB-G: 20.13 cm2 (95% C.I. 18.10–22.15) and the smallest by CHV1-infected L14/EP713: 2.49 cm2 (95% C.I. 1.59–3.39). Surprisingly, the size of the lesions induced by other CHV1-infected strains fell somewhere in between these extremes. The size of induced lesions was dependent on the population of origin as well and ranged from 11.60 cm2 (95% C.I. 9.87–13.33) for stems from the Moslavačka gora population to 17.75 cm2 (95% C.I. 15.63–19.87) for stems from Ozalj.

A GIS-Based Framework to Analyze the Behavior of Urban Greenery During Heatwaves Using Satellite Data

This work proposes a new unsupervised method to evaluate the behavior of urban green areas in the presence of heatwave scenarios by analyzing three indices extracted from satellite data: the Normalized Difference Vegetation Index (NDVI), the Normalized Difference Moisture Index (NDMI), and Land Surface Temperature (LST). The aim of this research is to analyze the behavior of urban vegetation types during heatwaves through the analysis of these three indices. To evaluate how these indices characterize urban green areas during heatwaves, an unsupervised classification method of the three indices is proposed that uses the Elbow method to determine the optimal number of classes and the Jenks classification algorithm. Each class is assigned a Gaussian fuzzy set and the green urban areas are classified using zonal statistics operators. The membership degree of the corresponding fuzzy set is calculated to assess the reliability of the classification. Finally, for each type of greenery, the frequencies of types of green areas belonging to NDVI, NDMI, and LST classes are analyzed to evaluate their behavior during heatwaves. The framework was tested in an urban area consisting of the city of Naples (Italy). The results show that some types of greenery, such as deciduous forests and olive groves, are more efficient, in terms of health status and cooling effect, than other types of urban green areas during heatwaves; they are classified with NDVI and NDMI values of mainly High and Medium High, and maximum LST values of Medium Low. Conversely, uncultivated areas show critical behaviors during heatwaves; they are classified with maximum NDVI and NDMI values of Medium Low and maximum LST values of Medium High. The research results represent a support to urban planners and local municipalities in designing effective strategies and nature-based solutions to deal with heat waves in urban settlements.

Preliminary Studies on Seed Dormancy of Premna latifolia Roxb.

Background: Premna latifolia Roxb. (Lamiaceae) is a deciduous tree distributed in Africa to China, throughout Indochina and Malaysia, to Australia and the Pacific. The various parts of the plant (leaves, stem bark and root) is reported to have numerous medicinal properties. The lack of understanding and consideration of dormancy and germination traits of a species could result in unsuccessful restoration programme. The aim of the present study was to investigate the nature of seed dormancy of P. latifolia that have low germination in natural conditions. Materials and Methods: To determine the type of seed dormancy, water imbibition and germination test were performed. Seeds were treated by mechanical scarification, Gibberellic acid (GA) and combined treatment of scarification and GA before allowing them to imbibe in moist paper. Results: All the treated (mechanically scarified, GA treated, mechanically scarified + GA treated) and untreated (control) seeds imbibed water for 4 days, however the maximum imbibition occurred in scarified seed + GA3 500ppm for 24 h (40.3%) followed by mechanically scarified seeds (38.7%). Higher germination was observed in scarified seeds + GA3 500ppm for 24 h (44%) followed by GA3 500ppm for 24 h (29.3%). Whereas, lowest germination was observed in untreated seeds (6.6%). Conclusion: Seeds possess non -deep physiological dormancy which requires scarification and treatment of GA to enhance germination.

Limited impact of soil inocula from arbuscular and ectomycorrhizal-dominated sites on root morphology and growth of four tree seedling species from a temperate deciduous forest

Limited impact of soil inocula from arbuscular and ectomycorrhizal-dominated sites on root morphology and growth of four tree seedling species from a temperate deciduous forest

Landscape-level analysis of disturbance regimes in Chinnar Wildlife Sanctuary, a protected area of Western Ghats, India

ABSTRACT The degradation, fragmentation, and depletion of forests have occurred all over the world, simultaneously, with the exponential growth of the human population. The present study assesses the forest disturbance regime of Chinnar Wildlife Sanctuary (CWS), an ecologically significant biodiversity hotspot of Western Ghats (WG) through satellite image-based forest and land cover mapping and landscape metric analysis. The on-screen analysis based on field knowledge, facilitated, mapping of seven types of vegetation cover. The majority of forest area was covered by dry deciduous forest (39.2 km2 or 43.15%), whereas the minimum area was covered by disturbed forest (2.05 km2 or 2.26%). The disturbance index (DI) was calculated by linearly combining the land use land cover (LULC), road and settlement closeness, fire risk, porosity, patchiness interspersion, normalized difference vegetation index (NDVI), and fragmentation through a probabilistic weight-based linear additive model. The values of the DI (120–310) reclassified into four classes viz. low, moderate, high, and very high. The results revealed that 74.62 km2 (82.52%) of the forest area has low to moderate disturbance, while the rest of the forest area that is 15.8 km2 (17.48%) has high to very high disturbance. These analyses are based on remote sensing and GIS crucial for forest conservation and management strategies formulation.

Characterization of cocoa (Theobroma cacao L.) wood branches as a potential resource for paper production

For sustainable use of lignocellulosic resources, pruned tree branches of cocoa, one of the major evergreen tropical tree crops with significant economic importance worldwide, were investigated as a potential source of kraft pulp. This study determined the chemical composition, fiber dimensions, kraft pulp, and paper properties of the cocoa tree (Theobroma cacao L.) branches compared to the deciduous trees traditionally used in the paper industry. A handsheet of cocoa pulp showed promising results with narrow fiber length distribution, high paper density, and high mechanical strength. The yield of kraft pulp made from cocoa branches wood was lower. The tensile and burst indices of cocoa pulp handsheet were 2 and 2.5 times higher than that of hardwood traditionally used in the paper industry. These results suggest a potential use of cocoa (Theobroma cacao L.) tree branch wood for pulp production using the kraft process.

Thinning Impacts on Carbon and Water Budgets in a Temperate Deciduous Forest Ecosystem

Among various forest management activities, thinning is a prevalent treatment that affects tree growth and living biomass. Increased moisture and light availability may also enhance the mineralization of litter and dead wood organic matter, impacting soil carbon stocks. Thinning may also affect the services forests provide, including water production and nutrient cycling. The impacts of thinning on water yield and carbon stocks have been well documented around the globe while targeting mainly one of these ecosystem services. Our experimental paired catchment study covers both and puts forward long term results. We assessed the carbon stock changes caused by two slight thinning treatments together with the impacts on water yield in experimental paired catchments of 71.9 (W–I) and 77.5 hectares (W–IV) in Istanbul, Türkiye. The null hypothesis was that the slight thinnings did not affect the water yield and carbon stocks significantly. On the carbon stock part, we calibrated and parametrized the CBM–CF3 model with field measurements to simulate changes in carbon stocks of mixed deciduous forest stands. The intensities of the treatments (thinnings) were 11% and 18% of the basal area, performed in 1986 and 2011, respectively. We found that, while C stocks decreased by around 30 tons per hectare during the 1986–2020 period, the water yield was enhanced by approximately 25 mm/yr in the treatment catchment compared to the control watershed during the four-year post-treatment period. This amount of streamflow increase was around 10 percent of the average water yield of the catchments. It was concluded that there was a detectable increase in water yield during the following four years of the slight thinning treatments, while the reduction in abovegound carbon stocks continued for more than three decades.

Multi-Omics Analysis Reveals the Mechanism by Which RpACBP3 Overexpression Contributes to the Response of Robinia pseudoacacia to Pb Stress

Acyl-CoA-binding protein (ACBP) genes have been implicated in lead enrichment and translocation in plants; however, the mechanisms by which these genes contribute to the response to heavy metal stress in various taxa have not been determined. In this study, the molecular mechanisms underlying the response of Robinia pseudoacacia, an economically important deciduous tree, to Pb stress were examined using transcriptomic and metabolomic analyses. RpACBP3 overexpression increased Pb enrichment, translocation, and tolerance. After Pb stress for 3 days, 1125 differentially expressed genes (DEGs) and 485 differentially accumulated metabolites (DAMs) were identified between wild-type and RpACBP3-overexpressing R. pseudoacacia strains; after Pb stress for 45 days, 1746 DEGs and 341 DAMs were identified. Joint omics analyses showed that the DEGs and DAMs were co-enriched in α-linoleic acid metabolism and flavonoid biosynthesis pathways. In particular, DEGs and DAMs involved in α-linoleic acid metabolism and flavonoid biosynthesis were up- and down-regulated, respectively. Moreover, RpACBP3 overexpression enhanced the ability to scavenge reactive oxygen species and repair cell membranes under stress by regulating LOX gene expression and increasing the phosphatidylcholine content, thereby improving the tolerance to Pb stress. These findings lay a theoretical foundation for the future application of RpACBP3 genes in plant germplasm resource creation and phytoremediation of Pb contaminated soil in which R. pseudoacacia grow.

Leaf nutrient basis for the differentiation of photosynthetic traits between subtropical evergreen and deciduous trees.

Compared to evergreens, deciduous tree species usually have higher photosynthetic efficiency to complete vegetative and reproductive growth in a shorter growing season. However, the nutrient basis for the differentiation of photosynthesis functional traits between evergreen and deciduous tree species has not yet been clarified. Thirty evergreen and twenty deciduous angiosperm tree species from a subtropical common garden were compared in terms of photosynthetic traits and leaf nutrients. Generally, their differences in area-based photosynthetic capacity were uncorrelated with area-based leaf nutrient content but were caused by the fraction of nitrogen allocated to photosynthetic components. By comparison, the differences in mass-based photosynthetic capacity were more correlated with leaf nitrogen content than leaf phosphorus and potassium content. Convergence in phosphorus and potassium constraints to photosynthesis occurred in deciduous tree species but not in evergreen tree species. Furthermore, leaf C/N ratio played a more significant role than leaf mass per area in determining the differentiation of photosynthetic traits between evergreen and deciduous groups. Our findings provide insight into the nutrient basis for photosynthetic carbon gain and functional strategies across trees species.

Exploring Cheura [Diploknema Butyracea (Roxb.) H. J. Lam]: Unveiling its Ethnomedicinal Heritage, Phytochemical Composition, Pharmacological Properties, and Innovative Applications.

Diploknema butyracea (Roxb) H.J Lam, also referred as "Kalpavriksha", is commonly known as Gophal, Cheura, or Indian butter tree. It is a deciduous tree with straight trunks of 15-20 m in height and white-yellow-coloured fragile flowers with fragrance, found at altitudes of 300-1500 m in the sub-Himalayan region of India, China, Nepal, and Bhutan. Diploknema have 11 taxa and 8 species, out of which 3 species are found in Uttarakhand hills, Sikkim, Darjeeling, Arunachal Pradesh, and Assam. The tree holds significant economic importance, serving various purposes within ethnic communities. Its high lipid content makes it valuable for food, medicine, construction, and the production of various value-added products. The ethno-pharmacological applications encompass treating rheumatism, burns, asthma, and various skin conditions. The plant's different components-bark, leaves, flowers, seeds, and fruits-contain a diverse array of phytoconstituents, including alkaloids, tannins, flavonoids, steroids, terpenoids, and palmitic acid, along with essential nutrients like sodium, calcium, potassium, iron, magnesium, zinc, and various sugars which shows diverse pharmacological and therapeutic activities. Beyond traditional uses, Diploknema is important for diverse industrial application in pharmaceuticals, confectionery, nutraceuticals, and cosmetics. Present paper is an attempt to understand comprehensive details on different aspects of this plant to explore new avenues for various value-added products.

The Impact of Different Types of Trees on Annual Thermal Comfort in Hot Summer and Cold Winter Areas

Trees positively improve the annual thermal comfort of the built environment in tropical areas, where climate change is slight throughout the year. However, for areas with high changes in climate all year, the current studies have only explored the summer cooling performance of trees without the impact of different types of trees on annual thermal comfort, especially in cold seasons. Therefore, to quantify the impacts and scientifically guide the optimization of green space layout in hot summer and cold winter areas, this study selected Changsha City as the study area and analyzed how the annual thermal comfort is affected by evergreen trees and deciduous trees, which are two common types of trees in hot summer and cold winter areas. The analytical results indicated that the difference in the effect of deciduous and evergreen trees on outdoor thermal comfort was insignificant in summer, where the difference in the monthly mean PET for the three summer months was slight, being 0.28 °C, 0.14 °C, and 0.29 °C, respectively. However, evergreen trees greatly exacerbated winter cold compared to deciduous trees, with a monthly mean PET decrease by nearly 1.0 °C and an hourly PET reduced by up to 3.57 °C. The difference is mainly attributed to the absorption and reflection of solar radiation by the tree canopy, as well as the cooling and humidifying effect of the tree leaf. In hot summer and cold winter areas, outdoor thermal comfort is still in the “comfortable” and “slightly warm” acceptable stage despite the warming effect of deciduous trees in the spring and autumn seasons. Planting evergreen trees is an inevitable thermal mitigation choice for tropical areas. However, for the areas with high annual climate change, such as hot summer and cold winter areas in China, a change in empirical tree planting patterns and selecting deciduous trees where appropriate will improve year-round outdoor thermal comfort.

Vertical profile measurements for ammonia in a Japanese deciduous forest using denuder sampling technique: ammonia emissions near the forest floor

Ammonia (NH3) has received considerable attention as a major reduced nitrogen. However, accurate estimates of the deposition amount are difficult due to its complex behavior characterized by bidirectional exchange between the atmosphere and the surface. We observed the vertical profile of NH3 concentration in a deciduous forest in Japan for 1 year to further advance the studies on NH3 bidirectional exchange in Asia, especially focusing on the process near the forest floor. The observation period lasted from September 29, 2020, to September 28, 2021, including leafy and leafless periods. Using the denuder sampling technique, we measured NH3 concentration in the forest at three heights (above the forest canopy, 30 m, and near the forest floor, 2 m and 0.2 m). NH3 concentrations tended to be highest at the top of the canopy (30 m). Focusing on the concentration near the forest floor, the concentrations at 0.2 m were frequently higher than those at 2 m regardless of the leafy and leafless period, thus suggesting NH3 emissions from the forest floor. NH3 concentration near the forest floor showed strong positive correlations with air temperature during the leafy period. The NH3 emissions from the forest floor during the leafy period were possibly due to the decomposition of leaf litter with increased air temperature. The decrease in leaf area index might induced the increase in NH3 concentration and emission. NH3 emission during the leafless period was also possibly dependent on the state of the deposition surface, apart from air temperature, relative humidity, and leaf area index.Graphical

Maastrichtian palaeoenvironments and palaeoclimate reconstruction in southern South America (Patagonia, Argentina) based on fossil fungi and algae using open data resources

ABSTRACT The use of non-pollen palynomorphs (NPP), particularly fossil fungi and algae, as palaeobiological proxies for Late Cretaceous palaeoenvironmental and palaeoclimatic reconstructions of warm-to-hot greenhouse conditions, can enhance our understanding of climate change impacts on modern Patagonian environments. This study aimed to reconstruct the Maastrichtian palaeoenvironment and palaeoclimate in the Cañadón Asfalto Basin (CAB, Chubut Province) by testing these NPPs as proxies using the Nearest Living Relative method (NLR). Moreover, using modern ecological requirements from open-source databases, such as GBIF and processing it with an open-source, cross-platform tool like QGIS, linked with Köppen-Geiger shapefiles, provided evidence of climate-driven palaeo-distribution patterns of fungal and algal diversity at CAB. Applying modern ecological requirements and biogeographic distribution data, we reconstructed the palaeoclimate as temperate with evenly distributed precipitation and warm summers, corresponding to the Cfb climate zone in Köppen-Geiger classifications. Additionally, our methodology produced reliable results regarding Cenozoic floras’ physiognomies based on fossil fungi, revealing a transition from sparsely wooded areas with palms and prairies to complex forest ecosystems with palms, deciduous trees, and shrubland. Furthermore, testing Cretaceous algae with the NLR method, for the first time, provided comprehensive insights into past water body characteristics, including trophic state and water quality.

Unravelling 6000 years of interplay among environmental changes, anthropogenic activities, and Vesuvius eruptions in the upper Sarno Plain (Campania, Italy)

Abstract Pollen analysis was carried out on the infilling succession of the Fossa San Vito sinkhole, at the NE foothills of the Sarno Plain (Italy). Four 14C dates and six tephra layers constrain the pollen sequence between ca. 6000 and 500 cal yr BP. A forested environment, with a few signs of human activities, characterizes the pre-protohistoric period (ca. 6000–2750 yr BP). Stability of the arboreal pollen grains to non-arboreal pollen grains (AP/NAP) curve is due to climate-related opposite oscillations of deciduous and evergreen forest. In this period, the pyroclastic products from Neapolitan volcanoes that reached the upper Sarno Plain seem to have affected neither vegetation nor human activities. In the archaic and classic periods (ca. 2750–1500 yr BP), intensive deforestation and increase in anthropogenic indicators indicate the occurrence of grazing and crop activities managed by the main urban centers located in the plain: Pompeii, Stabiae, and Nuceria. After the Pompeii eruption in CE 79, a rapid re-afforestation and decline in all anthropogenic indicators testify to the temporary abandonment of the area, linked to the disastrous demise of the main economic centers. The upper plain was repopulated and exploited in the Late Ancient and Middle Ages (ca. 1700–500 yr BP), as indicated by the increase in all crop and grazing indicators.

Mycolichenological portrait of alder forest: alpha diversity revealed in 1ha plot in the Samursky National Park (Republic of Dagestan, Russia)

Aim. Xylobiont fungi and lichens determine the sustainability of forest ecosystems, but their species richness and taxonomic diversity depend on the forest type and the degree of its disturbance. Alder forests, characterised by a specific set of ecological conditions, represent poorly studied habitats in terms of myco‐ and lichenobiota. The aim of this work was to determine the species composition of aphyllophoroid fungi and epiphytic lichens on a 1 ha sample plot in a lowland floodplain forest dominated by Alnus glutinosa in the Delta Samura area of the Samursky National Park.The authors collected basidiomata of lignicolous fungi and lichen specimens on a sample plot of 1 ha during field studies. The surveyed forest area is represented by a community dominated by Alnus glutinosa and lianas. The material was identified using light microscopy techniques and a standard set of chemical reactions.Thirty‐three species of aphyllophoroid fungi (Basidiomycota) and 53 species of lichens and allied fungi (Ascomycota) were identified. Among them, 16 species (48 %) of aphyllophoroid fungi were recorded for the first time for the Samursky National Park, including six species revealed for the first time for the Republic of Dagestan, of which three species (Donkia pulcherrima, Phanerochaete cumulodentata and Sertulicium granuliferum) were new to the Northern Caucasus. Lichen species Arthothelium ruanum, Bacidia arceutina and Graphis pulverulenta are listed for the first time for the Republic of Dagestan. At the same time, 21 species of lichenized fungi were recorded for the first time on Alnus glutinosa within the region.The taxonomical structure of aphyllophoroid fungi revealed reflects the spring period of basidiomata‐based field study. The predominance of corticioid fungi and revealing hydnoid species among morphological groups may be indicative of the generally wetter habitats of alder forests in comparison to other forest types distributed in the studied area. Most of species were recorded on dead wood of Alnus glutinosa as the main forest‐forming tree. At the same time, each of other substrates (Carpinus betulus and Corylus avellana) turned out to be the habitat of species new to the region. The group of fungal species developing on large‐scale substrate units, represented by fallen trunks, was the most prevalent and is to be considered as more vulnarable in a case of anthropogenical habitat disturbance. The majority of epiphytic lichens discovered on alder are not specific to this woody substrate, except for single findings. These species belong to epiphytes inhabiting the Samur forest, the core of the lichenobiota of which is composed of thermophilous lichens of lowland deciduous forests. In our opinion, the relict character of the forest, which is an isolated and rather small forest massif with relatively homogeneous climatic conditions, causes a high similarity between the species composition of lichens revealed in the surveyed sample plot with Alnus glutinosa and previously studied plots dominated by Carpinus betulus, Populus alba, and Quercus robur.

Assessment of Fuel Wood Consumption and Its Effect on Forest Depletion in Dry Deciduous Forest of Haliyal Taluk, Uttar Kannada District, India

Forest plays a significant role in extending wood and non-wood resources around the world, Forests provide ecosystem services, such as timber, food, fuel, fodder, non-wood products and shelter, which are essential for human well-being. However the increasing demand and pressure on forest resources majorly like fuel wood lead to degradation of forests. Hence it is important to assess the fuel wood consumption pattern and its impact on the forest for sustainable management of resources. In the present study, Haliyal taluk was selected, the area which is under rapid urbanization, surrounded by dry deciduous forest and the people living in that area depend on forest for fuel wood consumption. The study examined fuel wood collection among different farmer categories: large, medium, small, and landless. Results from the semi structured questioner survey revealed that medium farmers collected the most fuel wood. The total fuel wood consumption in Haliyal taluk was 5232.2 tonnes, with an average household consumption of 2.60 quintals of fuel wood per year. Major preferred tree species for fuel wood were Xylia xylocarpa, Terminalia tomentosa and Lagerstroemia lanceolata, which are valued for their high energy content, favourable burning characteristics and availability. For estimation of forest degradation Randomized Block Design was used and the study classified the forest vegetation into four crown density classes: Very Dense Forest (T1), Moderately Dense Forest (T2), Open Forest (T3), and Scrub (T4). Data was collected from 20 plots (20 x 20 m each) across these classes to measure forest depletion. The results showed that statistically significant differences in degradation levels, with the highest depletion (31.56 m³/ha) in very dense forests followed by moderate depletion (6.75 m³/ha) in moderately dense forests and the lowest (2.4 m³/ha) in scrub forests. This pattern reflects greater anthropogenic pressures and higher fuel wood availability in more dense forest types.

First Report of Alternaria alternata Causing Leaf Spot of Magnolia delavayi in China

Magnolia delavayi, known as Delavay's magnolia or Chinese evergreen magnolia, is an enchanting deciduous tree native to southwestern China (Xu et al. 2020). In March 2024, leaf spots were observed on M. delavayi plants at Qujing Normal University (25.527°N, 103.744°E), Qujing, Yunnan, China. About 80% of the trees (n=92) on campus were infected, with infected leaves accounting for about 10 to 30% of all leaves. The leaf spots exhibited a round or oval shape, with a yellowish-brown center and a brown outer ring. As the spots expanded, the leaves gradually dried up and fell off. Five symptomatic leaves were collected from the middle sections of five diseased plants. Tissue samples (5 × 5 mm) were excised from the lesion margins and subjected to surface sterilization in 75% ethanol for 30 seconds and 1% sodium hypochlorite for 60 seconds. They were then rinsed three times with sterile water and dried on sterilized paper. The samples were plated on potato dextrose agar (PDA) and incubated at 28 °C for 3 days. Developing colonies were retransferred to new PDA and purified by the hyphal tip technique (Senanayake et al. 2020). Two representative isolates (SYL1 and SYL2) were selected and confirmed to be the same species based on morphological characteristics and molecular identification. On PDA, the fungal colonies initially appeared grayish-white and turned dark gray over time with cotton-like aerial hyphae on the surface. The conidia are light brown, pear-shaped or rod-shaped, with 1 to 4 transverse septa and 0 to 1 oblique septa, and measured 5.4 to 12.7 × 10.4 to 25.7 μm (n = 50). Molecular identification was performed by partial sequencing of the internal transcribed spacer (ITS: ITS4/ITS5) region, glyceraldehyde 3-phosphate dehydrogenase (GAPDH:gpd1/gpd2), translation elongation factor 1-alpha (TEF1:EF1/EF2), and Alternaria major allergen (Alt a 1:Alt-for/Alt-rev) (Woudenberg et al. 2015). The resulting sequences of ITS (PP951882, PP951883), GAPDH (PP968942, PP968943), Alt a 1(PP968940, PP968941) and TEF1 (PP968938, PP968939) were deposited in GenBank. BLAST analyses revealed that the sequences from these two isolates showed 100% identity with those from Alternaria alternata strains for each gene. Additionally, a multigene phylogenetic analysis revealed a distinct group containing SYL1, SYL2 and other A. alternata strains with a posterior probability of 100%. The morphological and phylogenetic evidence collectively suggested that the two isolates belonged to A. alternata. Strain SYL1 was used for the pathogenicity test on three 5-year-old M. delavayi plants on campus. Three healthy leaves of each plant were punctured with a sterile needle and sprayed with 20 μl of spore suspension (1 × 106 conidia/ml). Similarly, another nine wounded leaves were sprayed with 20 μl of sterile water. All inoculated leaves were enclosed in plastic bags for 48 hours. Disease symptoms were observed on leaves inoculated with spore suspension after 7 days, while control plants remained healthy. This experiment was repeated three times with same results. To fulfill Koch’s postulates, A. alternata was reisolated from the inoculated leaves and identified based on morphological characteristics and ITS sequencing. To our knowledge, this is the first report of A. alternata causing leaf spot on M. delavayi in China. This research expands the known host range of this pathogen and provides relevant information to the design of disease management strategies.

Diversity and composition of Coreidae (Hemiptera: Heteroptera) in a seasonal forest of central Mexico

ABSTRACT The diversity of the Coreidae family in the tropical deciduous forest (TDF) of Coaxitlán, Tlaquiltenango, Morelos, Mexico, is presented. From the collection carried out during an annual cycle, 34 species, 18 genera, 8 tribes, and 2 subfamilies were recorded. The sample coverage was 99% and according to the Chao 1 richness estimator, 90% of the species in the study area were represented. The greatest richness, abundance, and diversity occur during the months of greatest rainfall. The faunal similarities between months show groupings in species composition corresponding to the TDF’s seasonality. The community structure reflects a high diversity, integrated by a high proportion (>60%) of species with a low abundance. The data show that the diversity of Coreidae in the TDF is high, but the environmental heterogeneity of this type of vegetation suggests that the species composition could still be different in other sites, which shows the need to continue documenting it.