The effects of increasing amounts of sodium hydroxide at a range of temperatures and sodium chlorite at 70°C on the extraction and delignification of arabinoxylan from destarched wheat bran under laboratory conditions have been examined. Optimal conditions for extraction and delignification were found to be pretreatments of sodium hydroxide and sodium chlorite both at 40% (percentage expressed on destarched bran ratio) and at 40°C for extraction. These conditions were then applied on a pilot scale. The extracted arabinoxylan was purified by a microfiltration and dried by an atomization system. The yields of purified arabinoxylan obtained under laboratory and pilot conditions were similar and equal, respectively, to 16 and 13%. Also the purity (xylose and arabinose content expressed on destarched bran ratio) was similar in both procedures and equal to 75%.