De Regt Research Articles

L. Koehler and W. Baumgartner, The Hebrew and Aramaic Lexicon of the Old Testament. 3rd edn, revised by W. Baumgartner and J.J. Stamm with assistance from B. Hartmann, Z. Ben-ḥayyim, B. Hartmann, E.Y. Kutscher and P. Reymond, translated and edited under the supervision of M.E.J. Richardson in collaboration with G.J. Jongeling-Vos and L.J. de Regt. Vol. I: ʾ-ḥ, pp. cvi

L. Koehler and W. Baumgartner, The Hebrew and Aramaic Lexicon of the Old Testament. 3rd edn, revised by W. Baumgartner and J.J. Stamm with assistance from B. Hartmann, Z. Ben-ḥayyim, B. Hartmann, E.Y. Kutscher and P. Reymond, translated and edited under the supervision of M.E.J. Richardson in collaboration with G.J. Jongeling-Vos and L.J. de Regt. Vol. I: ʾ-ḥ, pp. cvi

Herman C.D.G. De Regt, Representing the World by Scientific Theories: The Case for Scientific Realism. Tilburg, Tilburg University Press, 1994, fl 165.00

Previous articleNext article No AccessReviewsHerman C.D.G. De Regt, Representing the World by Scientific Theories: The Case for Scientific Realism. Tilburg, Tilburg University Press, 1994, fl 165.00James LadymanJames LadymanDepartment of Philosophy King's College, Division of History and Philosophy of Science and Department of Philosophy, University of Leeds London Search for more articles by this author PDFPDF PLUS Add to favoritesDownload CitationTrack CitationsPermissionsReprints Share onFacebookTwitterLinkedInRedditEmail SectionsMoreDetailsFiguresReferencesCited by The British Journal for the Philosophy of Science Volume 47, Number 3September 1996 Society: The British Society for the Philosophy of Science Views: 3Total views on this site Views: 3Total views on this site Article DOIhttps://doi.org/10.1093/bjps/47.3.487 Views: 3Total views on this site © 1996 by The Author. All rights reserved.PDF download Crossref reports no articles citing this article.

The rate-limiting step in yeast PGK1 mRNA degradation is an endonucleolytic cleavage in the 3'-terminal part of the coding region.

Insertion of an 18-nucleotide-long poly(G) tract into the 3'-terminal untranslated region of yeast phosphoglycerate kinase (PGK1) mRNA increases its chemical half-life by about a factor of 2 (P. Vreken, R. Van der Veen, V. C. H. F. de Regt, A. L. de Maat, R. J. Planta, and H. A. Raué, Biochimie 73:729-737, 1991). In this report, we show that this insertion also causes the accumulation of a degradation intermediate extending from the poly(G) sequence down to the transcription termination site. Reverse transcription and S1 nuclease mapping experiments demonstrated that this intermediate is the product of shorter-lived primary fragments resulting from endonucleolytic cleavage immediately downstream from the U residue of either of two 5'-GGUG-3' sequences present between positions 1100 and 1200 close to the 3' terminus (position 1251) of the coding sequence. Similar endonucleolytic cleavages appear to initiate degradation of wild-type PGK1 mRNA. Insertion of a poly(G) tract just upstream from the AUG start codon resulted in the accumulation of a 5'-terminal degradation intermediate extending from the insertion to the 1100-1200 region. RNase H degradation in the presence of oligo(dT) demonstrated that the wild-type and mutant PGK1 mRNAs are deadenylated prior to endonucleolytic cleavage and that the half-life of the poly(A) tail is three- to sixfold lower than that of the remainder of the mRNA. Thus, the endonucleolytic cleavage constitutes the rate-limiting step in degradation of both wild-type and mutant PGK1 transcripts, and the resulting fragments are degraded by a 5'----3' exonuclease, which appears to be severely retarded by a poly(G) sequence.

The rate-limiting step in yeast PGK1 mRNA degradation is an endonucleolytic cleavage in the 3'-terminal part of the coding region.

Insertion of an 18-nucleotide-long poly(G) tract into the 3'-terminal untranslated region of yeast phosphoglycerate kinase (PGK1) mRNA increases its chemical half-life by about a factor of 2 (P. Vreken, R. Van der Veen, V. C. H. F. de Regt, A. L. de Maat, R. J. Planta, and H. A. Raué, Biochimie 73:729-737, 1991). In this report, we show that this insertion also causes the accumulation of a degradation intermediate extending from the poly(G) sequence down to the transcription termination site. Reverse transcription and S1 nuclease mapping experiments demonstrated that this intermediate is the product of shorter-lived primary fragments resulting from endonucleolytic cleavage immediately downstream from the U residue of either of two 5'-GGUG-3' sequences present between positions 1100 and 1200 close to the 3' terminus (position 1251) of the coding sequence. Similar endonucleolytic cleavages appear to initiate degradation of wild-type PGK1 mRNA. Insertion of a poly(G) tract just upstream from the AUG start codon resulted in the accumulation of a 5'-terminal degradation intermediate extending from the insertion to the 1100-1200 region. RNase H degradation in the presence of oligo(dT) demonstrated that the wild-type and mutant PGK1 mRNAs are deadenylated prior to endonucleolytic cleavage and that the half-life of the poly(A) tail is three- to sixfold lower than that of the remainder of the mRNA. Thus, the endonucleolytic cleavage constitutes the rate-limiting step in degradation of both wild-type and mutant PGK1 transcripts, and the resulting fragments are degraded by a 5'----3' exonuclease, which appears to be severely retarded by a poly(G) sequence.

Progressive neurological deficit in children with spina bifida aperta: J.H. Begeer, M.J. Meihuizen de Regt, I. HogenEsch, et al. Z Kinderchir 41:13–15, (December), 1986 (suppl)

Progressive neurological deficit in children with spina bifida aperta: J.H. Begeer, M.J. Meihuizen de Regt, I. HogenEsch, et al. Z Kinderchir 41:13–15, (December), 1986 (suppl)

Power Changes and Self-Respect: A Comparison of Two Cases of Established-Outsider Relations

This article is based on two studies of outsiders in an emancipation process. 1) The first one deals with homosexuals (van Stolk and de Regt, 1979), and the second with women (van Stolk and Wouters, 1983); both studies were carried out in a social service organization. Most of the homosexuals sought counselling because of difficulties in accepting themselves; they suffered from a lack of self-respect. The women, predominantly working-class, had been taken into a crisis centre after having run away from their partners. In doing so most of them had given way to pressure due to an increase in self-respect; in effect they could no longer tolerate the way they had been treated. The problems of both the homosexuals and the runaway women are examined in the context of a decreasing inequality of power in the relations between homosexuals and heterosexuals, and between men and women respectively. With the help of Norbert Elias’s established-outsider theory, the relation between women and men is compared to that between homosexuals and heterosexuals. The main focus is on the connection between changes in their mutual balance of power, in the respect shown them socially and in their self-respect. The comparison is preceded by a short survey of the main conclusions of the two empirical studies.

Private Schools in France: Evolution of a System

Previous articleNext article No AccessPrivate Schools in France: Evolution of a SystemRichard TeeseRichard Teese Search for more articles by this author PDFPDF PLUS Add to favoritesDownload CitationTrack CitationsPermissionsReprints Share onFacebookTwitterLinkedInRedditEmail SectionsMoreDetailsFiguresReferencesCited by Comparative Education Review Volume 30, Number 2May, 1986 Sponsored by the Comparative and International Education Society Article DOIhttps://doi.org/10.1086/446591 Views: 8Total views on this site Citations: 4Citations are reported from Crossref Copyright 1986 The Comparative and International Education SocietyPDF download Crossref reports the following articles citing this article:Gabriel Heller-Sahlgren Smart but unhappy: Independent-school competition and the wellbeing-efficiency trade-off in education, Economics of Education Review 62 (Feb 2018): 66–81.https://doi.org/10.1016/j.econedurev.2017.10.005Tammy Harel Ben-Shahar Distributive Justice in Education and Conflicting Interests: Not (Remotely) as Bad as you Think, Journal of Philosophy of Education 49, no.44 (Feb 2015): 491–509.https://doi.org/10.1111/1467-9752.12122Ali de Regt *, Don Weenink When negotiation fails: private education as a disciplinary strategy, Journal of Education Policy 20, no.11 (Jan 2005): 59–80.https://doi.org/10.1080/0268093042000322838Richard Teese The political functions of the central administration of Catholic education in France, Journal of Education Policy 4, no.22 (Jan 1989): 103–114.https://doi.org/10.1080/0268093890040202