De Ouro Research Articles

Use of pseudomonas fluorecens isolated from the Serra de Ouro Branco State Park/Minas Gerais - Brazil in the biodegradation of residual automotive lubricating oils

About 2% of the oil consumed worldwide is related to the production of automotive and industrial lubricating oils. The pollution derived from these oils in aquatic and terrestrial environments is responsible for several ecological and social problems due to their toxic, mutagenic, and carcinogenic properties. Thus, studies related to bioremediation are of importance, and the use of biological treatments, such as biodegradation, is a viable and effective alternative for the treatment of these compounds. The present study aimed to evaluate the biodegradation performance of residual automotive lubricating oils using the lipases Pseudomonas fluorecens obtained in bioprospecting carried out in the Serra do Ouro Branco State Park, Minas Gerais. Through the colorimetric method using the redox indicator 2.6-dichlorophenol-indophenol (DCPIP), the biodegradability of residual oils was monitored. The results obtained demonstrated the potential of the selected bacteria, since they degraded approximately 61.74 to 83.8 % of the waste studied.

From laboratories to Museum: the trajectory of the mineralogy collection of the Escola de Minas de Ouro Preto

The paper presented is the result of the author's doctoral research, which included an excerpt on the formation of the mineralogy collection of the former Escola de Minas de Ouro Preto, currently the Escola de Minas da Universidade Federal de Ouro Preto. This analysis aims to map the trajectory of the collection from its entry to the museumization of the samples. As a methodological path, the exploratory research collected information in online repositories and in the archives of the Escola de Minas da UFOP and the Museu de Ciência e Técnica da Escola de Minas da UFOP. This study sought to answer how the set of minerals present in the Mineralogy Office contributed to the training of mining engineers in the 19th and early 20th centuries. The results show a movement of collections from other national and foreign institutions and their didactic use even after their museumization in 1930 1. Introdução .

From laboratories to Museum: the trajectory of the mineralogy collection of the Escola de Minas de Ouro Preto

The paper presented is the result of the author's doctoral research, which included an excerpt on the formation of the mineralogy collection of the former Escola de Minas de Ouro Preto, currently the Escola de Minas da Universidade Federal de Ouro Preto. This analysis aims to map the trajectory of the collection from its entry to the museumization of the samples. As a methodological path, the exploratory research collected information in online repositories and in the archives of the Escola de Minas da UFOP and the Museu de Ciência e Técnica da Escola de Minas da UFOP. This study sought to answer how the set of minerals present in the Mineralogy Office contributed to the training of mining engineers in the 19th and early 20th centuries. The results show a movement of collections from other national and foreign institutions and their didactic use even after their museumization in 1930.

Metabolic profile and body condition score of Crioulo horses finalists in the Freio de Ouro competition

The aim of this study was to assess the body condition score (BCS) and metabolic profile of finalists in the Freio de Ouro competition, stratified by sex. This study examined 84 Crioulo horses, comprising 43 non-pregnant mares and 41 stallions. Morphometric measurements, including BCS, cresty neck score (CNS), neck and heart girth, and assessments of fat depth at the neck and at the tail base, were conducted. Additional derived measurements such as the percentage of body fat (%BF), body mass index (BMI), fat mass (FM), fat-free mass (FFM), heart girth:height ratio (HG:HW), and neck circumference:height ratio (NC:HW) were calculated. A subgroup of 53 animals was selected for the evaluation of metabolic parameters, including triglycerides, cholesterol, low-density lipoprotein (LDL), high-density lipoprotein (HDL), lactate dehydrogenase (LDH), creatine kinase (CK), and adiponectin.In terms of BCS, 21% of participants were classified as overweight, with 49% having CNS≥3. Females exhibited greater fat deposition at the tail base and neck, along with higher %BF, FM, and HG:HW ratio, and lower NC:HWcompared to males. Among metabolic variables, females showed higher concentrations of LDH, LDL, CK, and lower concentrations of adiponectin than males. No differences were observed in morphometric measurements between participants that qualified for the final day of the competition and those which were disqualified. The study revealed that despite the rigorous physical demands and preparation required for the Freio de Ouro competition, a percentage of participants were overweight, displaying increased regional fat deposition in the neck. Although no metabolic changes related to increased adiposity were identified, the findings indicate a predisposition to the development of metabolic alterations. Additionally, females exhibited a greater accumulation of fat than males.

Cardiovascular training adaptations in Criollo breed horses: biochemical markers and morphofunctional parameters

Abstract The aim of the present study was to investigate the cardiovascular morphofunctional and biochemical training adaptations of Criollo breed horses. Thirty-one animals were evaluated, arranged in two groups – untrained horses, used as a control group (n = 17); trained horses, in training for the Freio de Ouro competition (n = 14). The means and standard deviation of echocardiographic, electrocardiographic and biochemical variables were measured and, subsequently, compared between groups of trained and untrained animals using Student’s t test, considering . Trained horses had lower biochemical levels of CK, CKMB and cTnI I when compared to untrained animals. The echocardiographic variables interventricular septum during diastole (IVSd), left ventricular free wall during systole (LVFWs), left ventricular free wall during diastole (LVFWd), left ventricular mass (LVMass), diameter of the aorta (Ao) and diameter of the left atrium (LA) have shown higher values in trained horses compared to untrained. Duration of the T wave was the only electrocardiographic variable that was influenced by training, showing higher values for untrained animals. Therefore, there is influence of training on biochemical variables, in addition to on morphological and functional echocardiographic/electrocardiographic characters in trained Criollo breed horses, characterised by concentric cardiac hypertrophy, when compared to untrained individuals. In addition, a majority of normal sinus heart rhythm was observed in the animals evaluated.

Population and Slavery in Vila Rica de Ouro Preto (1712-1770)

The discovery of gold at the end of the 17th century and of diamonds during the 1730s in central-southern Brazil caused profound dislocation in the slave trades, turning the Minas Gerais region into an unprecedented pole of attraction for migrant and slave labor. In this work, we perform a sociodemographic analysis of the slave population in Vila Rica de Ouro Preto, the capital of the captaincy of Minas Gerais, in a period ranging from 1712 to 1770. For this purpose, and based on previous literature, we use the data series gathered from the two parishes that made up the urban geography of the region, as well as the notarial records preserved at the Arquivo Histórico do Museu da Inconfidência - Casa do Pilar. The reconstruction of families and the study of the relationship between the sociodemographic data of the slave contingent and those of the rest of the population, the processes of manumission, and the evolution of the slave import market have allowed us to prove the dependence of the slave population on slave trade.

Semente de milho crioula promove incremento nos aspectos produtivos e nutricionais da forragem hidropônica

ABSTRACT The aimed of this study to assess the productivity and nutritional characteristics of hydroponic corn forage derived from bico de ouro creole seeds and AL-Bandeirante. Two treatments (T), each representing a corn seed variety, were randomly allocated to beds filled with a wood sawdust substrate: T1 (n=5) - bico de ouro creole seed variety, and T2 (n=5) - AL-Bandeirante hybrid seed variety. On the 13th day, forage was collected to assess seedling biometry, green and dry mass production, and bromatological analyses. The data were subjected to statistical analysis, with a 5% significance level. The creole seed variety yielded higher seedling height (17.53 cm), root length (10.19 cm), stem (5.37 cm), leaf (12.17 cm), and greater production of green mass (7.22 kg/m²) and dry matter (2.05kg/m²) (P<0.05). The AL-Bandeirante corn forage had higher dry matter content (39.18%), mineral matter (2.33%), non-fiber carbohydrate (31.88%), and estimated total digestible nutrients (68.14%) (P<0.05), while the creole corn forage had higher organic matter content (99.45%), ethereal extract (19.53%), neutral detergent fiber (45.56%), and cellulose (15.46%) (P<0.05). Crude protein, acid detergent fiber, and lignin were similar between the treatments (P>0.05). Despite the AL-Bandeirante variety yielding a higher concentration of certain nutrients, its forage productivity was significantly low. Therefore, under the conditions of this study, the use of the creole seed variety is recommended.

Age, skin color, self-rated health, and depression associated with co-occurrence of obesogenic behaviors in university students: a cross-sectional study.

The university context plays an important role in the health-disease process since students are potentially vulnerable to obesogenic behaviors that can influence long-term health. To estimate the prevalence of and factors associated with the co-occurrence of obesogenic behaviors among university students. This was a cross-sectional study at a Brazilian public university. This study was conducted with all university students in the first and second semesters of 2019 at Universidade Federal de Ouro Preto, Minas Gerais, Brazil. Data were collected between April and September 2019, using a self-administered questionnaire. The outcome was the co-occurrence of obesogenic behaviors, measured as the sum of three risk behaviors: inadequate eating practices, leisure-time physical inactivity, and sedentary behavior. A Venn diagram was used to evaluate the simultaneous occurrence of risk behaviors. Pearson's chi-square test and multivariate logistic regression were used for statistical analyses. A total of 351 students participated in the study. Inadequate eating practices constituted the most prevalent isolated risk behavior (80.6%), which was also the most prevalent when combined with sedentary behavior (23.6%). University students aged 20 years or younger, with non-white skin color, poor self-rated health, and symptoms of depression had increased chances of simultaneous occurrence of obesogenic behaviors. These findings highlight the importance of developing and implementing actions to reduce combined obesogenic behaviors in the university environment. Institutions should focus on creating an environment that promotes health-protective behaviors such as physical activity and healthy eating.

The anatomical model collection at the Universidade Federal de Ouro Preto's Museum of Pharmacy

The Ouro Preto School of Pharmacy was founded in 1839 and was the first pharmacy school in Latin America independent from a medical school. At the end of the nineteenth century, it had a collection of French anatomical models made by Deyrolle, Dr. Auzoux, and Vasseur-Tramod, many produced from wax or papier-mâché. This project involved recovering, identifying, cleaning, restoring, and exhibiting seventeen models found in various facilities from Universidade Federal de Ouro Preto. The models in good condition were exhibited in the Museum of Pharmacy (where this work was carried out) as part of the teaching collection for the Ouro Preto pharmacy course.

Conformidade no manuseio do leite materno cru doado aos bancos de leite humano em relação à qualidade microbiológica

Abstract Objectives: microbiological quality of raw human milk (HM) from donors at the HMB (Human Milk Bank) of Santa Casa de Misericórdia de Ouro Preto was evaluated. Methods: the microbiological quality of milk from each of 10 donors in triplicate (30 samples) was evaluated by enumeration of Staphylococcus aureus and total aerobic mesophiles (surface plating) and total and thermotolerant coliforms (Most Probable Number - MPN). A questionnaire and a checklist were applied after the end of sample collection to verify the compliance of milk extraction and storage procedures by the donors. Results: inadequacies in the procedures for expression and storage of donated HM were observed. The presence of all microorganisms studied were verified in the milk of all donors, and in some cases exceeded the safety limits. The checklist indicated that some necessary hygienic-sanitary procedures were not carefully performed, making it important to properly guide the donors. Conclusions: this study points to an unfavorable scenario for the safety and quality of donated HM, and it is important to encourage further studies to be conducted so that the Brazilian network of HMB adopts stricter criteria for instructions on procedures and support for HM donors.

Anatomical description of the brain wax models of Museu da Pharmacia de Ouro Preto.

In 1839, the Escola de Farmácia de Ouro Preto pioneered the teaching of pharmaceutical sciences in Brazil. At the end of the 19th century, the Escola de Farmácia possessed a French collection of anatomical models, some made of wax and paper-mâché. The models were a critical part of teaching anatomy, particularly in an era of paradigm changes about how the human brain works. The present study aimed to anatomically describe the brain models through a comparative analysis with the current anatomical description. Comparative analysis of the brain models with modern anatomical descriptions. In the individual analysis of the wax models, we verified excellent anatomical accuracy of the cortical and subcortical regions. Our results identified internal structures, like the basal ganglia and white matter. Compared with modern anatomical books and websites, the wax brain models have high scientific quality. The models of the present study gave students hands-on experience of human anatomy in the 19th century. Nowadays, the models are part of the memory of Universidade Federal de Ouro Preto and Museu da Pharmacia de Ouro Preto . The collection of wax models shows the appreciation of neuroanatomy teaching at the turn of the century concomitant with advances in neurology and anatomy around the world.

From theatre to streets: the dynamics of shows in Ouro Preto, Brazil (1870-1900)

ABSTRACT Ouro Preto, a city known worldwide for its baroque architecture, had its status as the capital of the State of Minas Gerais, Brazil, threatened by republican ideals that aimed at progress and modernization, fighting colonial characteristics and values. Aiming to adjust and try to sustain its position, it implemented improvements with interventions from urban perimeter to habits. This paper examines how entertainments gained attention from local governments in the midst of modernization attempts, between 1870 and 1900. Documentary research was carried out at the Arquivo Público Municipal de Ouro Preto (Ouro Preto Municipal Public Archive), prioritizing municipal sources. Shows of varied natures, as dramatic, equestrian and bullfights, were held in different spaces, with those happening in closed places, especially in the theatre, being privileged regarding open shows, considered barbaric and uncivilized. Among the actions, there were even projects to build new theatres in the city. The shows were income providers for the Câmara Municipal (City Council), behaviour influencers, and thermometers of civility and local progress.

Photosynthetic Responses, Growth, Production, and Tolerance of Traditional Varieties of Cowpea under Salt Stress.

HighlightsSalinity tolerance among traditional varieties of cowpea is variable.High net photosynthesis is observed in cowpea-tolerant varieties under salt stress.The decrease in photosynthesis is caused by stomatal restriction and low photochemical efficiency.Cowpea varieties are similar in photochemical efficiency and photochemical quenching.The decrease in pod number and seeds per pod are the main effects of salinity on cowpea production.Cowpea is the main subsistence crop—protein source—for the Brazilian semi-arid region. The use of salt-stress-tolerant varieties can improve crop yields. We evaluated the effect of irrigation with brackish water on the growth, photosynthetic responses, production, and tolerance of fifteen traditional varieties of cowpea. The experiment was conducted in randomized blocks, in a 15 × 2 factorial scheme, composed of 15 traditional varieties of cowpea and two salinity levels of irrigation water (0.5 and 4.5 dS m−1), with five replicates. Plants were grown in pots containing 10 dm3 of soil for 80 days. The reduction in the photosynthetic rate of cowpea varieties occurs mainly due to the decrease in stomatal conductance caused by salt stress. Salt stress increased the electron transport rate and photochemical quenching of cowpea varieties, but stress-tolerant varieties increased the CO2 assimilation rate and instantaneous carboxylation efficiency. The Ceará, Costela de Vaca, Pingo de Ouro, Ovo de Peru, and Sempre Verde varieties are tolerant to salt stress. Salt stress decreases 26% of the production of tolerant varieties to salt stress and 54% of susceptible varieties. The present findings show the existence of variability for saline stress tolerance in traditional varieties of cowpea and that Ceará, Costela de Vaca, Pingo de Ouro, and Ovo de Peru varieties are more suitable for crops irrigated with saline water.

Ritmo y equilibrio aspectos básicos para el desarrollo de las habilidades motoras gruesas en niños de 5 años

This research originates as a product of the integration of three subjects Psychomotricity, Stimulation and Curricular Design, whose purpose is to improve motor skills in 5-year-old children, starting from this principle and to strengthen the paradigm on the relationship that exists between rhythm , balance and gross motor skills, a bibliographic review is made in the first instance, which subsequently leads to the development of activities to increase mature motor patterns, which strengthen the acquisition of more complex skills in relation to muscle control, body coordination , locomotion and balance using rhythm as a strategy, for this it is necessary to know the degree of body awareness, postural control, balance, conformation and location of the body that children have. From this perspective, an activity plan for gross motor development was drawn up based on what was established by the LOEI, where rhythm and balance are the channel for strengthening in this area, to meet the objective requested support to the Cordão de Ouro group, who provided the children with the required ages to carry out the investigative work. During the execution of the proposed activities, it was found that rhythm offers advantages that improve balance and at the same time favor the concept and body image, the expression of their emotions, creativity, and the development of their motor skills. In short, rhythm and balance are a regulating valve that leads to the comprehensive training of children.

PROJETO ATLETAS DE OURO®: UMA INOVAÇÃO NA DETECÇÃO DE TALENTOS ESPORTIVOS

Every young person has a potential that must be evaluated to be properly developed. The Projeto Atletas de Ouro® aims to estimate the sporting potential of schoolchildren and young athletes. The aim of this study was to present the main results obtained at the Military College of Juiz de Fora (CMJF).  Between 2015 and 2019, 2917 individual diagnoses were performed in students aged 10 to 19 years. 14% of the students had high sporting potential and were three times more likely to be medalists in competition.  One of the students became a professional soccer athlete. The GoldFit – Atletas de Ouro System proved to be valid and reliable for identifying talents in the school. The actions of orientation and talent development were positive in the school Physical Education and in the performance of the CMJF in school competitions. The goal is to expand the Projeto Atletas de Ouro® to other schools and programs as a model for identifying and developing sporting talents.

Absenteeism due to mental and behavioral disorder in employees of a federal university.

Mental and behavioral disorders (MBD) are one of the main causes of absence from work in Brazil and worldwide. To analyze the prevalence of absence from work according to the International Classification of Diseases, 10th revision, with stratification of disease defined as "Mental and Behavioral Disorders", in permanent employees of the Federal University of Ouro Preto and its relationships with sociodemographic and occupational determinants, during the period from 2011 to 2019. An epidemiological, descriptive, and analytical study, with a cross-sectional design and quantitative approach was conducted using primary and secondary data. The population consisted of federal public sector workers who were granted ML to treat their own health during a 9-year period. Analyses were performed using descriptive and bivariate statistics. The Wilcoxon (Mann-Whitney) and Poisson tests were used to assess the existence of associations between variables. 733 medical records of employees eligible according to the inclusion criteria were analyzed. There was a rising trend in ML rates over the 9-year period. Of the sample, 23.2% (n = 170) were absent from work due to mental and behavioral disorders - females accounted for 57.6% and administrative technicians in education for 62.3%. In the multivariate analysis (Poisson test), only the outcome "time of first ML due to mental and behavioral disorders" was associated with the variable "time working at the Universidade Federal de Ouro Preto". The high prevalence of mental and behavioral disorders found in this investigation is an alert to the magnitude of the problem, highlighting the urgency of implementing measures to detect psychosocial risk factors, whether associated with work or not.

Performance of cowpea weevil Callosobruchus maculatus (F.) infesting seeds of different Vigna unguiculata (L.) Walpers genotypes: The association between bruchid resistance and chitin binding proteins

Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae) is an important pest of stored cowpea seeds ( Vigna unguiculata) (L.) Walpers and the infestation of these seeds by the pest is a limitation to their production. Many studies have identified V. unguiculata genotypes that were resistant to the insect infestation. These resistant genotypes can lead to a reduction on the use of insecticides, avoiding environmental and food contamination. In this work, we evaluated the resistance or susceptibility of different V. unguiculata genotypes to infestation by C. maculatus . Our results showed that the number of eggs laid by C. maculatus varied from 6 to 13 eggs per seed among genotypes. Almost all the eggs were viable, and the larvae hatched at 5 days after oviposition (DAO). Larval survival was decreased in some genotypes, where only about 40% of the larvae survived. The mass of the larvae at 20 DAO ranged from 8.8 mg to 6.4 mg/larva. The emergence of adults was also decreased in some genotypes. The seed mass consumed by the larvae varied between 40 and 70% and affected seed germinability. Proteins similar to vicilins, glycinin, and CPRD22 were identified in V. unguiculata Pingo de Ouro 1-5-4 and MNC06-909-76 lines. Chitin-binding proteins were isolated from some genotypes and were toxic to the larvae at 1%. The docking of vicilin with chitin [(NAG) 4 ] showed negative value of affinity energy, indicating a spontaneous binding. The vicilin interacted with (NAG) 4 by hydrogen bonds, saline bridge, and hydrophobic interactions. These results suggest that part of the interference in the insect development, observed in some cowpea genotypes, may be due to the presence of chitin-binding vicilins. • Cowpea genotypes interfered with cowpea weevil survival. • The cowpea infestation by cowpea weevil affected seed germination. • Proteins such vicilins, glycinin, and CPRD22 were identified in cowpea genotypes. • Cowpea chitin-binding proteins, at 1%, were toxic to cowpea weevil larvae. • Vicilin-chitin docking indicated a spontaneous binding, with a negative ΔG value.

Polyphenols for improvement of inflammation and symptoms in rheumatic diseases: systematic review.

Rheumatic diseases (RDs) are a group of pathological conditions characterized by inflammation and functional disability. There is evidence suggesting that regular consumption of polyphenols has therapeutic effects capable of relieving RD symptoms. To synthesize data from randomized controlled trials on administration of polyphenols and their effects on RD activity. Systematic review conducted at Universidade Federal de Ouro Preto, Minas Gerais, Brazil. A systematic search was conducted in the databases PubMed (Medline), LILACS (BVS), IBECS (BVS), CUMED (BVS), BINACIS (BVS), EMBASE, Web of Science and Cochrane Library and in the grey literature. The present study followed a PRISMA-P checklist. In total, 646 articles were considered potentially eligible, of which 33 were then subjected to complete reading. Out of these, 17 randomized controlled trials articles were selected to form the final sample. Among these 17 articles, 64.71% assessed osteoarthritis (n = 11), 23.53% rheumatoid arthritis (n = 4), 5.88% rheumatoid arthritis and fibromyalgia (n = 1) and 5.88% osteoarthritis and rheumatoid (n = 1). Intake of polyphenol showed positive effects in most of the studies assessed (94.12%): it improved pain (64.70%) and inflammation (58.82%). Polyphenols are potential allies for treating RD activity. However, the range of polyphenol sources administered was a limitation of this review, as also was the lack of information about the methodological characteristics of the studies evaluated. Thus, further primary studies are needed in order to evaluate the effects of polyphenol consumption for reducing RD activity. PROSPERO - CRD42020145349.

Water-deficit tolerance of landrace and improved corn genotypes

Abstract The objective of this work was to evaluate the yield, leaf area, stomatal conductance, photosynthesis, transpiration, and critical fraction of transpirable soil water (FTSWc) of hybrid and landrace corn (Zea mays) genotypes, as affected by the water deficit characterized by the FTSW. Two experiments were carried out in the 2015/2016 and 2016/2017 crop years: one in the field and the other in a greenhouse. The following genotypes were evaluated in irrigated and nonirrigated systems: the Cinquentinha and Bico de Ouro landraces and the AS 1573PRO improved cultivar. Yield in the field was higher for 'AS 1573PRO', followed by Bico de Ouro and Cinquentinha in both evaluation years. Water deficit reduces stomatal opening, photosynthesis, and transpiration rate, resulting in the total closure of the stomata of 'AS 1573PRO' and Bico de Ouro, and in the partial closure of those of Cinquentinha. There was a reduction in the leaf area of 'AS 1573PRO', Bico de Ouro, and Cinquentinha under water deficit during the reproductive period. In the first crop year under water deficit conditions, 'AS 1573PRO' and Cinquentinha show a high tolerance to water deficit with a FTSWc of 0.71, and 'AS 1573PRO' also shows the highest tolerance in the second crop year with a FTSWc of 0.73. Moreover, Bico de Ouro is sensitive to water deficit in the two crop years.

Lipase production by microorganisms isolated from the Serra de Ouro Branco State Park.

Lipases are hydrolases used in various sectors such as the food, pharmaceutical and chemical synthesis industries. In this study, epiphytic microorganisms were isolated from the Serra of Ouro Branco State Park (Minas Gerais, Brazil) and were subsequently evaluated for their ability to produce extracellular lipases. Among the 46 isolated strains, 25 presented positive results for lipase production in the agar plate screening assay. Two of these strains that expressed the highest diffusion halos, were genetically identified as Serratia marcescens and Pseudomonas fluorescens and catalogued in the Tropical Cultures Collection from the André Tosello Foundation/Brazil as CCT 7796 and CCT 7797, respectively. The fermentation growth kinetics indicated that the maximum extracellular lipase activities were achieved between 96 and 120h of cultivation. The highest lipolytic activity for both strains was observed at an optimum temperature and pH of 37°C and 7.0, respectively. At these conditions, the lipase activity detected in the crude enzymatic extract of both strains was close to 15.0 U/mL. We consider that these species are promising lipase producers for industrial applications.