Abstract Objectives The objective was to explore the influence of health behaviors on health-related quality of life (HRQOL) among college undergraduate students. Methods A convenience sample of college students completed an online survey consisting of the Center for Disease Control's HRQOL modules, perceived stress scale, sleep questionnaire, and the National Cancer Institute Fruit and Vegetable (FV) Screener. Body mass index (BMI) was calculated using self-reported height and weight. Independent t-test were used to compare HRQOL between students who consumed above/below average fruit and vegetable intake. Two multiple regression analyses were used to determine health factors that predicted better HRQOL. Results Participants (N = 655) were 19.8 ± 1.5 years old, female (63%), and white (84%). The average FV intake was 2.2 servings per day. Students who consumed more than the average intake of FV, reported more days/month (d/m) feeling healthy and full of energy (11.9 ± 8.6 vs. 8.9 ± 7.9, P < 0.001), and reported less d/m of poor mental health (8.5 ± 8.1 vs. 11.1 ± 9.8, P < 0.001) compared to those who consumed less than the average. Two predictors explained 38.5% of variance in d/m when mental health was not good (r2 = 0.38, F (2,639) = 200.11, P < 0.001): perceived stress (β = 0.61, P < 0.001) and FV intake (β = −0.08, P < 0.05). Four predictors explained 30.0% of the variance in d/m feeling happy and full of energy (r2 = 0.29, F (4,549) = 58.6, P < 0.001): perceived stress (β = −0.46, P < 0.001), BMI (β = −0.11, P < 0.01), FV intake (β = 0.16, P < 0.001), and hours of sleep (β = 0.08, P < 0.05). Conclusions This data shows that students who consumed above average FV intake reported more days of feeling happy and full of energy and less days of poor mental health, and that modifiable behaviors (FV intake, stress, sleep) impact HRQOL of young adults. This research provides justification for college aimed wellness interventions to include both nutrition education and mental health resources to improve overall HRQOL. Funding Sources This project was supported by the USDA National Institute of Food and Agriculture, Hatch project number #ME0022104 through the Maine Agricultural & Forest Experiment Station. NJ Agriculture Experiment Station.