Days Of Ice Storage Research Articles

Lipids from cephalothorax and hepatopancreas of Pacific white shrimp (Litopenaeus vannamei): Compositions and deterioration as affected by iced storage

Lipids from cephalothorax and hepatopancreas of Pacific white shrimp (Litopenaeus vannamei) stored in ice for up to 6 days were extracted and characterised. The extraction yields of lipids from hepatopancreas (10.65-12.64%) were higher than those from cephalothorax (2.59-2.88%). However, no changes in the extraction yield were observed during the storage (p>0.05). The carotenoid contents of lipids from cephalothorax and hepatopancreas slightly increased within the first 2 and 4 days of iced storage (p<0.05), respectively, but decreased thereafter (p<0.05). With increasing storage time, a progressive formation of hydroperoxide was found as evidenced by the increase in the absorbance band at 3600-3200 cm(-1) in Fourier transform infrared (FTIR) spectra, and increased peroxide values (PVs) (p<0.05). The increases in thiobarbituric acid reactive substances (TBARS), p-anisidine value (AnV) and free fatty acid (FFA) content of lipids were noticeable when iced storage time increased (p<0.05). Those changes indicated that lipid oxidation and hydrolysis occurred in both samples. Phospholipids (PL) were the major components in lipids from cephalothorax (82.51% of total lipids). Nevertheless, lipids from hepatopancreas contained triglyceride (TG) and PL as the dominant components (45.35% and 38.03% of total lipids, respectively). A decrease in the TG content with a concomitant increase in free fatty acid was observed at the end of storage (day 6) (p<0.05). Decreases in unsaturated fatty acids, especially eicosapentaenoic acid (EPA; C20:5(n-3)) and docosahexaenoic acid (DHA; C22:6(n-3)) were noticeable at day 6 of storage (p<0.05). Thus, the extended storage time resulted in the enhanced deterioration of extracted lipids.

Effects of pre-slaughter stress on proteolytic enzyme activities and muscle quality of farmed Atlantic cod (Gadus morhua).

Farmed Atlantic cod were subjected to a combination of stressors in a holding tank before being killed, pre rigor filleted and stored in ice. At slaughter, a higher level of stress was confirmed by blood physiology analyses. This was further associated with significantly reduced muscle pH and somewhat elevated muscle collagenase-like activity in the stressed fish, whereas no differences in cathepsin-like activities were found. After 5 days of iced storage, the stressed fish had significantly lower water holding capacity, reduced hardness and yellowish colour compared to the control group, and no differences in the other parameters investigated. Independent of pre-slaughter stress, the activities of cathepsin B- and B/L-like enzymes increased and activities of cathepsin D/E- and collagenase-like enzymes decreased with storage.

Retardation of myoglobin and haemoglobin-mediated lipid oxidation in washed bighead carp by phenolic compounds

Antioxidative activities of phenolic compounds (caffeic acid, gallic acid and tannic acid; 200 ppm) in washed mince (pH 6), with added myoglobin (Mb) and haemoglobin (Hb), from bighead carp (Hypophthalmichthys nobilis), during 9 days of iced storage, were studied. Tannic acid exhibited the preventive effect on discolouration of washed mince containing Mb or Hb during storage (P<0.05). High peroxide value (PV) was found and large amount of, thiobarbituric acid-reactive substances (TBARS) and hexanal were formed in washed mince containing haem proteins, especially Hb. As determined by apo Streptococcal haem-associated protein, Hb had the lower haem affinity than Mb. Phenolic compounds, especially caffeic acid and gallic acid, could lower lipid oxidation induced by Mb or Hb throughout storage (P<0.05). Prevention of haem release, as well as inhibition of lipid oxidation induced by haem proteins with selected phenolic compounds, should be an alternative means in lowering discolouration and lipid oxidation in fish muscle.

Biogenic amines, amino acids and microflora changes in Indian mackerel (Rastrellinger kanagurta) stored at ambient (25-29°C) and ice temperature (0°C).

Biogenic amines formation in Indian mackerel of tropical region was investigated during storage at ambient (25-29°C) and ice temperature (0°C) in relation with changes of amino acids content and amines forming bacteria. All amines increased significantly during storage at two temperatures except for spermidine and spermine. Histamine concentration of 363.5ppm was detected after 16h stored at ambient temperature. Aerobic plate count of fish stored at ambient temperature reached 6.98 log CFUg(-1) after 16h, close to the upper limit (7 log CFUg(-1)) suggested by International Commission on the Microbiological Specifications for Foods (ICMSF). However, proper icing procedure retarded the formation of histamine effectively, resulting only 8.31ppm after 16days of ice storage. Aerobic plate count of 5.99 and 7.72 log CFUg(-1) were recorded for fish stored in ice after 16days and ambient temperature after 20h, respectively. Histamine exhibited high correlation with histidine (r(2) = -0.963, P < 0.01) as well as cadaverine with lysine (r(2) = -0.750, P < 0.05). However, tyramine-tyrosine demonstrated a weaker relationship (r(2) = -0.138, P > 0.05). As storage time progressed, the amines forming bacteria grew significantly except for that stored in ice.

Effect of ice storage on the functional properties of proteins from a few species of fresh water fish (Indian major carps) with special emphasis on gel forming ability.

In the present study the effect of ice storage on physico-chemical and functional properties of proteins from Indian major carps with special emphasis on gel forming ability have been assessed for a period of 22days. The solubility profile of proteins in high ionic strength buffer and calcium adenosine triphosphatase (ATPase) enzyme activity reduced significantly (p < 0.05), while that of total volatile base nitrogen (TVB-N) increased significantly (p < 0.05) at the end of 22days of ice storage. The major protein fraction showed association-dissociation-denaturation phenomenon during ice storage as revealed by gel filtration profile and viscosity measurements. The gel forming ability of three fish species both in fresh and during different periods of ice storage was assessed by measuring the gel strength of heat induced gel. Among the three species the gel strength of the gel obtained from Catla catla and Cirrhinus mrigala was higher (586 and 561g.cm) than the gel obtained from Labeo rohita (395g.cm) in fresh condition. The gel forming ability of three species was significantly affected (p < 0.05) during ice storage. The TVB-N values of fish meat as a function of ice storage was within the prescribed limit up to 17days of the ice storage.

Biochemical, physical and sensory quality of ice-stored Atlantic cod (Gadus morhua) as affected by pre-slaughter stress, percussion stunning and AQUI-S™ anaesthesia

Biochemical, physical and sensory quality of farmed Atlantic cod subjected to percussion stunning (control), anaesthesia (AQUI-S™) and excessive exercise (30 min chasing before slaughter, ‘stressed’) were analysed after 7 days of ice storage. The white muscle energy status (initial pH, muscle twitches and high-energy phosphates) revealed that the fish were truly representatives of rested (percussion stunned and anaesthetised) and stressed cod. Sensory evaluation showed that the fillets of cod exposed to percussion stunning and AQUI-S™ anaesthesia prior slaughter had slightly higher whiteness scores, and that the fillets of AQUI-S™ anaesthetised cod had slightly shinier surfaces than the fillets of cod exposed to pre-slaughter stress. Furthermore, fillets of anaesthetised (AQUI-S™) cod had significantly higher inosine monophosphate (IMP) contents and lower K-values than fillets of cod exposed to pre-slaughter stress, after 7 days of ice storage. Pre-slaughter stress did to some extent affect fillet colour immediately after killing and after ice storage. Otherwise, no significant effects of stress were observed with regard to biochemical, physical or sensory quality (ultimate pH, water content, drip loss, water holding capacity, texture and gaping) of farmed Atlantic cod.

Quality Indices of Squid (Photololigo duvaucelii) and Cuttlefish (Sepia aculeata) Stored in Ice

The objective quality indices of squid (Photololigo duvaucelii) and cuttlefish (Sepia aculeata) stored in ice were compared with the subjective counterparts. Sensory (overall quality rating, quality index method [QIM], and multisample difference test), microbiological (total viable count [TVC], psychrophilic count), chemical (trichloroacetic acid-soluble peptide [TCA-soluble peptide], trimethylamine nitrogen [TMA-N], total volatile bases nitrogen [TVB-N], ammonia content, and protein pattern), and physical analyses (expressible drip, color, and texture) were determined in both species during 16 days of iced storage. As storage time increased, TCA-soluble peptide, TVB, ammonia content, and expressible drip were increased (p < 0.05). TMA content was markedly increased after 10 and 8 days of storage in squid and cuttlefish, respectively. Both TVC and psychrophilic count increased as the storage time increased (p < 0.01). Myosin heavy chain was degraded with coincidental decrease in shear force and sensory texture during storage (p < 0.05). According to the overall rating score, shelf life of both species in ice was estimated to be 6 days. The increases in ammonia content and expressible drip were highly correlated with the decrease in overall quality rating and increase in quality index score of squid and cuttlefish (p < 0.01).

The Effects of Essential Oils Addition on the Quality of Wild and Farmed Sea Bream (Sparus aurata) Stored in Ice

The preservative effect of laurel (Laurus nobilis) and cumin (Cuminum cyminum) essential oils (EOs) on fresh vacuum-packed (VP) wild and farmed sea bream (Sparus aurata) fillets was evaluated during ice storage by microbiological, physicochemical and electrophoretic analyses. In the present study, wild (W) and farmed (F) fillet treatment included the following lots: control vacuum-packaged samples (WV and FV), VP with added EOs (0.5% v/w) of cumin (WVC and FVC), and of laurel (WVL and FVL). Wild and cultured fish were found to differ significantly in their muscle proximate compositions irrespective of fillet treatments with particularly higher fat and carbohydrate contents in farmed sea bream (4.82 and 0.32 g/100 g, respectively, vs. 1.53 and 0.22 g/100 g in wild fish). The treatment of wild and farmed sea bream fillets with laurel or with cumin EOs induced a decrease in bacterial growth by ca. 0.5 to 1 log cfu/g and in lipid oxidation by ca. 40% of TBA value, extending the shelf life of fish fillets by approximately 5 days of ice storage. However, the addition of EOs to VP fillets resulted in a reduced liquid holding capacity (LHC) throughout ice storage suggesting an early proteolysis initiation confirmed by the myofibrillar and sarcoplasmic electrophoretic profiles. Laurel and cumin EOs as natural and efficient antibacterial and antioxidant compounds can be used in conjunction with VP to enhance ice-stored sea bream quality.

Quality Assessment of Tiger Tooth Croaker (Otolithes ruber) During Ice Storage

Quality and shelf life of tiger tooth croaker (Otolithes ruber) during 19 days of iced storage were investigated in terms of sensory, chemical (total volatile basic nitrogen (TVB-N), thiobarbituric values (TBA), peroxide value (PV), free fatty acid (FFA), and pH) and microbiological (total viable count, TVC and total psychrotrophic count. TPC) aspects. Acceptability quality of Tiger tooth croaker determined by panelists was 15–17 days. Limiting factors for acceptability were eye, gills, peritoneum, smell of gills and abdominal cavity, internal organs and flesh. Bacterial loads in samples were higher than limiting level (3 × 106 CFU g−1) between 15–17 days of ice storage. Of the chemical indicators of spoilage, the initial value of pH was 6.71 and increased to 7.35 at the end of storage. TVB-N content was 15.31mg N/100g on day 0 and reached to 36.52 on day 19. The acceptability of tiger tooth croaker decreased as FFA, PV and TBA values increased (P < 0.05). The release of FFA increased from an initial value of 4.15 (expressed as % of oleic acid) to a final value of 12.75 during the storage period. The initial PV value was 11.69 meq/kg and it increased to 50.75 meq/kg on day 12 and then started to decrease to 35.82 meq/kg at the end of storage period. The initial and final values of TBA were 0.83 and 3.75, respectively.

Effect of Kiam (Cotylelobium lanceolatum Craib) Wood Extract on the Haemoglobin-Mediated Lipid Oxidation of Washed Asian Sea Bass Mince

Effect of ethanolic kiam wood extract (EKWE; 12 0.05% and 0.1%, w/w) on the retardation of haemoglobin-mediated lipid oxidation of washed Asian sea bass mince added without and with menhaden oil stored in ice up to 10 days was investigated. Samples containing haemoglobin had the highest peroxide value (PV) within the first 8 days and possessed the greater amount of thiobarbituric acid-reactive substances (TBARS), compared to those added with no haemoglobin (P 0.05). With addition of EKWE, lipid oxidation in washed mince added with haemoglobin and menhaden oil was retarded, especially when the higher level (0.1%; w/w) was used, as evidenced by lowered PV and TBARS. Formation of volatile lipid oxidation compounds was retarded in the sample containing oil and haemoglobin and treated with 0.1% EKWE, compared with that without the addition of EKWE after 10 days of storage in ice (P < 0.05). Sensory analysis revealed that samples containing haemoglobin without and with oil added had the highest intensity of fishy odour, compared to those treated with EKWE (0.05% and 0.1%) and the control sample (washed mince; P < 0.05). Thus, EKWE, especially at a level of 0.1%, could serve as a potential natural antioxidant in prevention of lipid oxidation and retardation of development of fishy odour and volatile lipid oxidation compounds in washed mince during iced storage.

Linseed oil inclusion in sea bream diets: Effect on fatty acid composition during ice storage

AbstractImpact on proximate and fatty acids patterns of partial and complete dietary replacement of fish oil (FO) by linseed oil (LO) within on‐growing period was evaluated on marketable‐size gilthead sea bream (Sparus aurata) during 17 days of ice storage. The practical diets were factorially designed as follows: 100% FO (FO), 70% LO + 30% LO (70LO) and 100% LO (100LO). On day 0 of ice storage, there were no significant differences in proximate composition among different treatments. Fish fed FO diet showed high saturated, monounsatured and highly unsaturated fatty acids proportions, whereas fillets from fish fed LO diets increased in 18:3n‐3 content and consequently n‐3 polyunsaturated content. Furthermore, fillet from fish fed FO diets had the highest n‐3/n‐6 ratio. Throughout ice storage no significant fatty acid losses were found, with fish fed LO diets maintaining the highest 18:3n‐3 levels.

Freshness assessment of ray fish stored in ice by biochemical, chemical and physical methods

Ray fish were caught, filleted, and stored in ice. Fillets were analysed for 18 days to determine the chemical, biochemical and physical changes and their relation to the muscle eating quality. Trimethylamine (TMA-N), total volatile bases (TVB-N), ATP content and breakdown products, K value, pH, texture, water-holding capacity (WHC) and colour changes were monitored. At the beginning of the study, the ray fish muscle showed a low concentration of ATP and a high value of inosine 5′-monophosphate (IMP). Regarding to the signs of freshness and deterioration, K value presented an exponential increase ( r2 = 0.95) with an initial value of 4.7% and a final value of 47.5%. Furthermore, the TBV-N and TMA-N significantly increased ( P < 0.05) during the storage in ice. As for the physical analysis whereas the texture changed ( P < 0.05); pH and the WHC were not affected ( P < 0.05). The overall results of this study indicated that the edible quality of ray fish muscle was maintained during at least 15 days of ice storage.

Retardation of haemoglobin-mediated lipid oxidation of Asian sea bass muscle by tannic acid during iced storage

Lipid oxidation mediated by haemoglobin from tilapia was monitored in washed Asian sea bass mince with and without added tannic acid (200 and 400 ppm) during 10 days of iced storage. Control samples (without tannic acid) had the highest peroxide value ( PV) within the first 2 days and possessed the greater amount of thiobarbituric acid-reactive substances (TBARS) throughout the storage of 10 days ( p < 0.05). With addition of tannic acid, the lipid oxidation of washed Asian sea bass mince was retarded, especially when the higher level (400 ppm) was used, as evidenced by lowered PV and TBARS. The retarded formation of volatile lipid oxidation products in the samples with added 400 ppm tannic acid was found. Sensory analysis revealed that samples with added 400 ppm tannic acid possessed lower fishy odour score, compared with the control sample and that with added 200 ppm tannic acid ( p < 0.05).

COST‐EFFECTIVE AND NONDESTRUCTIVE TEXTURAL ASSESSMENT OF FISH FRESHNESS VIA SYSTEM IDENTIFICATION PRINCIPLES

ABSTRACT This paper introduces a cost‐effective, rapid and nondestructive methodology exploiting system identification principles for monitoring the textural changes of fish during ice storage. Gilthead sea bream stored in ice for 0 (freshly killed) and 6 days are placed on a spring‐based weight scale and suffer a single, light and reproducible shock by a mallet. The reaction force, measured by the scale and recorded (throughout time) as an electrical signal, is analyzed via system identification principles for estimating the fish stiffness and damping ability in each freshness stage. Results highlight significant and highly repeatable reductions of the fish damping in 6 days of ice storage, due to textural changes undermining its ability of absorbing the shock‐induced energy. The methodology correlates well with organoleptic findings, describing significant elasticity loss in the 6th day ice‐stored fish. Thus, the proposed nondestructive methodology shows potential for cost‐effectively estimating textural changes in fish and for correlating them with freshness. PRACTICAL APPLICATIONSThe present methodology showed that a cost‐effective experimental setup used along with simple but carefully executed tests and basic principles of system identification may reliably measure physical changes in fish, even in high‐quality shelf‐life stages. Thus, it could serve as an improvement upon methods indicating degree of spoilage. Its potential practical applications include fisheries and the aquaculture industry, as well as fish freshness evaluation in lab tests.

Comparative studies on molecular changes and pro-oxidative activity of haemoglobin from different fish species as influenced by pH

Oxygenation, autoxidation as well as pro-oxidative activity of haemoglobins from tropical fish (Asian seabass, tilapia and grouper) as influenced by different pH (6, 6.5 and 7) were comparatively studied. Relative oxygenation of all haemoglobins decreased in the acidic conditions. Haemoglobin from Asian seabass was more oxygenated and stable against autoxidation at both pH 6 and 7, compared to those from tilapia and grouper. Haemoglobin from tilapia and grouper was fully oxidised at pH 6 after 120 h. Lipid oxidation of washed Asian seabass mince added with haemoglobin from various fish at different pH (6, 6.5 and 7) was monitored during 10 days of iced storage. Haemoglobins accelerated lipid oxidation more effectively at pH 6, compared with pH 6.5 and 7 as indicated by the higher peroxide value (PV) and thiobarbituric acid-reactive substances (TBARS). At the same pH values, haemoglobins from tilapia and grouper were more pro-oxidative than that from Asian seabass as evidenced by the higher PV and TBARS ( P < 0.05). Volatile lipid oxidation compounds detected by gas chromatography–mass spectrometry (GC–MS) were also formed at higher rate in the washed mince added with haemoglobin from tilapia or grouper with coincidental stronger fishy odour development, compared to the control and that added with haemoglobin from Asian seabass. Thus, lipid oxidation in fish muscle was more likely governed by haemoglobin, whose pro-oxidative activity varied depending upon the pH as well as molecular properties of haemoglobin.

Effect of bleeding on lipid oxidation and quality changes of Asian seabass ( Lates calcarifer) muscle during iced storage

Lipid oxidation, microbial load and fishy odour development in the slices of bled and un-bled Asian seabass during 15 days of iced storage were comparatively investigated. Bled samples showed the lower peroxide value (PV) and thiobarbituric acid-reactive substances (TBARS) throughout the storage period ( P < 0.05). Bleeding effectively lowered the total haem and non-haem iron contents in Asian seabass slices. The release of non-haem iron was pronounced in the un-bled samples during the storage. Solid phase micro-extraction coupled with gas chromatography and mass spectrometric (SPME-GCMS) analysis revealed that the bled samples stored in ice for 15 days contained the lower amount of volatile compounds. Heptanal, the major volatile compound detected in the un-bled samples, was four-fold higher than that of bled counterparts. The contents of aldehydic compounds, including hexanal, octanal, nonanal and nonenal were also higher in the former. Bled samples had the lower fishy odour, compared with the un-bled counterparts during storage ( P < 0.05). The lower total viable counts (TVC) and psychrophilic bacterial counts (PBC) were observed in the bled samples, in comparison with the un-bled ones ( P < 0.05). Thus, bleeding was a potential means in retarding lipid oxidation, fishy odour development, and microbial growth of Asian seabass slices during storage in ice.

Post-mortem Changes in Farmed Spotted Babylon Snail (Babylonia areolata) During Iced Storage

Post-mortem changes in farmed spotted Babylon snail stored in ice for 7 days were evaluated using nucleotide degradation products, K-value, total volatile base nitrogen (TVB-N), trimethylamine nitrogen (TMA-N), organic acid, free amino acids and biogenic amines. During a 7-day ice storage, K-value, TVB-N, TMA-N and organic acids contents increased with increasing storage time (p < 0.05). Changes in free amino acid, such as aspartic acid, serine, glutamic acid, glycine, arginine and proline were observed throughout the ice storage (p < 0.05), while total free amino acids were found to decrease significantly (p < 0.05). Biogenic amines found in snail muscle during ice storage were tyramine, putrescine, cadaverine, agmatine and histamine. Bacteria counts of snail muscle exceeded 7 log CFU/g, which was considered as the limit for acceptability after 7 days of iced storage. This result initiates the use of ice storage as a preliminary treatment for snails transported from farms.

Preslaughter stress and subsequent effect on flesh quality in farmed cod

The aim of this study was to investigate the effect of prehandling stress on the flesh quality of Atlantic cod (Gadus morhua). In order to stress the animal, water was reduced in the tank before a total of 30 fish were caught five at a time using a dipnet and held for 3 min, causing stress by hypoxia. This fish was compared with a control group (n=30) of fish exposed to anaesthetics directly in the tank. All fish were killed by a percussive blow to the head and exsanguinated and stored on ice before flesh quality was measured. Immediately after death and after ice storage for 3 and 8 days, 10 fish per group were measured for muscle pH, texture, fillet gaping, colour, drip loss and cathepsin D. Handling stress resulted in an initial increased fillet lightness, drip loss and decreased fillet shear force, although these differences did level off during ice storage. Stress caused by handling resulted in earlier onset of rigour mortis as compared with the control group, which reached maximum rigour tensions within 26 and 36 h postmortem respectively. We conclude that handling before harvest results in reduced time before entering rigour. After 8 days of ice storage, no effect of handling stress was seen on the muscle pH, flesh colour, fillet shear force, gaping score, drip loss or cathepsin D activity.

Changes in heme proteins and lipids associated with off-odour of seabass ( Lates calcarifer) and red tilapia ( Oreochromis mossambicus × O. niloticus) during iced storage

Changes in heme proteins and lipids associated with off-odour development in seabass ( Lates calcarifer) and red tilapia ( Oreochromis mossambicus × O. niloticus) muscles during 15 days of iced storage were studied. Fresh seabass contained the higher contents of myoglobin and heme iron, compared with red tilapia ( P < 0.05). An increase in metmyoglobin proportion was observed during storage. After 3 days of storage, a decreased heme iron content and a concomitant increase in non-heme iron content were noticeable in both fish ( p < 0.05). Oxidation of myoglobin and released non-heme iron were associated with lipid oxidation. The increases in oxidation products and free fatty acids were observed as the storage time progressed. Fishy and rancid odours were detected at day 6 of storage for both fish and a higher intensity was found in seabass muscle. Thus, the off-odour in fish muscle was mostly governed by lipid oxidation and species specific.

Post-mortem changes of muscle from fresh water prawn ( Macrobrachium rosenbergii) as influenced by spawning stages

Post-mortem changes of the muscle from pre- and post-spawned fresh water prawn ( Macrobrachium rosenbergii) were comparatively monitored during 7 days of iced storage. During the storage, the muscle of pre-spawned prawn had a greater value of trichloroacetic acid (TCA) soluble peptide, heat soluble collagen and pepsin-soluble collagen (PSC) contents than did post-spawned counterpart. Those components in the muscle of both prawns increased markedly after 3 days of storage ( p < 0.05). Conversely, insoluble collagen (ISC) content, shear force value and texture liking of both prawns decreased ( p < 0.05), indicating the softening of muscle. No changes in protein patterns were observed, except the decreased band intensity of 66 kDa protein in water soluble fraction of both prawns was found after 3 days of storage. T max and enthalpy of PSC from both prawns decreased during the first 4 days of storage ( p < 0.05), suggesting the degradation or denaturation of collagen in the muscle. Light microscopic study showed the lowering of intercellular connection of raw meat and higher gaping in cooked meat when the samples were stored for a longer time. Therefore, post-mortem characteristics of prawn muscle was affected by storage time and spawning stages.