Days Of Habituation Research Articles

A Conspecific Attenuates Prolactin Responses to Open-Field Exposure in Rats

Acute exposure to a novel environment, such as an open field, generally results in a prolactin surge, while several days of exposure to the open field is often characterized by a decline in prolactin. As exposure to the open field is a psychological stressor, altering the animal's interpretation of the event should alter prolactin levels. In the present study, juvenile male and female rats were habituated to the open field for 1 or 5 days prior to testing in the chamber alone or with a same-sex conspecific. Levels of prolactin were measured across all rats, and play (pins) was recorded for animals tested with a conspecific. Five days of habituation to the chamber resulted in lower levels of prolactin and more play than 1 day of habituation. Across both conditions of habituation, testing with a conspecific caused lower levels of prolactin than testing alone. In addition, play and prolactin were negatively correlated. The presence of a conspecific in a stressful situation may have reduced stress by altering the animal's negative interpretation of the open field. Further, as the intensity of the social interaction increased (more play), prolactin levels decreased.

Habituation of activity in an open field: A survey of inbred strains and F1 hybrids.

To determine if there is genetic variability in habituation of activity in an open field, we examined a number of inbred strains and F1 hybrids. Using 5-min exposures to a dark open field, we measured changes in exploratory behavior over 3 consecutive days in 129S3/SvImJ, A/J, BALB/ cByJ, C3H/HeJ, C57BL/6J, CBA/J, DBA/2J, FVB/NJ, (B6 x 129)F1/J, and (B6 x C3H) F1/J male and female mice. Strain differences in open-field activity and in habituation were evident. Some of the strain differences were further modified by sex. The strains and F1's could be separated into groups that increased, decreased, or did not modify their activities across testing sessions. In a second study, the effects of altering the floor surface on habituation were examined in male 129S3/SvImJ, C57BL/6J, DBA/2J, and (B6 x 129)F1/J mice. When the floor was altered after 3 consecutive days of habituation, increased activity levels were evident. There were strain differences in the responsiveness to the changes in the floor. These results confirm a genetic role in intersession habituation to an open field.

Plastic Color Bands Have No Detectable Short-Term Effects on White-Breasted Nuthatch Behavior

We examined whether plastic leg bands had short-term effects on foraging behavior in White-breasted Nuthatches (Sitta carolinensis), a species which, because of their very short legs and unusual habit of hanging upside-down on bark, may be particularly susceptible to deleterious effects of plastic bands. Ten nuthatches were outfitted with varying numbers of colored plastic bands and observed foraging in an aviary after two days of habituation. Wearing up to five plastic bands caused no detectable change in any of the variables measured: number of flights, body position, choice of foraging substrate, seed caching, seed hammering, and seed retrieval.

Effect of Egg Covering and Habitat on Nest Destruction by House Wrens

Birds nesting near House Wrens (Troglodytes aedon) risk having their eggs, nestlings, and nests destroyed. Damage by wrens may be reduced in Black-capped Chickadees (Parus atricapillus), Tufted Titmice (P. bicolor), and other parids by concealing eggs under nest material during the laying period, and in sympatric cavity-nesting species by nesting in different habitats from wrens. To test if eggs were protected by covering, prelaying wrens were challenged for 1 day with a set of two boxes placed 1 m from their nest, one with two artificial eggs (miniature marshmallows) lightly covered under fur, the other with two artificial eggs in an open cup. Results varied with stage of nest-building; in 41 trials where both exposed eggs were removed, covered eggs remained in only 4 of 15 (27%) trials near early nests containing few sticks, but in 17 of 26 (65%) trials near more advanced nests. To assess effects of nest site, a box with a cup nest was placed in each of three habitats 10 or 20 m from 29 wren nests. After 1 day of habituation, two artificial eggs were placed in each nest and left exposed for 1 day. Boxes in woodland interiors were less likely than boxes in fields and along edges to be visited by wrens at least once over 2 days (66 vs. 97% visited) and were less likely to have eggs removed (10 vs. 83% removed). Competitors for nesting cavities also may escape attacks by wrens through differences in breeding period, active defense of territories or nests, or renesting.

Role of drug-administration cues in the associative control of morphine tolerance in the rat.

This research investigated the role of injection procedures as a potential confound in the study of associative and nonassociative morphine tolerance development. Rats administered a series of morphine injections paired with a distinctive context environment can develop tolerance controlled associatively by the context. However, rats given morphine unpaired with the context may also develop some degree of tolerance. This study examined whether this tolerance represents an associative effect with animals using the injection ritual as a cue predictive of morphine delivery. Following 14 days of habituation to handling and injection stimuli, rats were given eight morphine injections (20 mg/kg, IP) explicitly paired or unpaired with a distinctive context. Animals were then tested for morphine analgesia in the context after either a 30-day rest condition or a 30-day period of daily saline injections. Analgesia was assessed by the tail-flick method, and tolerance was defined as the shift to the right of the dose-response curve of morphine-experienced relative to saline control animals. Paired animals across both retention conditions displayed tolerance, whereas tolerance retention in unpaired animals was observed only in those animals not given saline injections over the 30-day interval. Results support an associative interpretation of tolerance observed in unpaired conditions and suggest that the injection ritual may provide highly salient cues for the support of associative tolerance effects.

Alterations in the immunoreactivity for muscarinic acetylcholine receptors and colocalized PKC gamma in mouse hippocampus induced by spatial discrimination learning.

This study describes changes in the immunoreactivity for muscarinic acetylcholine receptors (mAChRs) in the hippocampus of mice in relation to spatial discrimination behavior, employing the monoclonal antibody M35 raised against purified bovine mAChR protein. Performance in a hole board in which the animals learned the pattern of 4 baited holes out of 16 holes served as the measure of spatial discrimination learning and memory. Twenty-six adult male house mice were used, divided into four groups. Three groups served as various controls: group N (naive; blank controls); group H (habituated; animals were introduced to the hole board with all holes baited for 5 consecutive days), and group P (pseudo-trained; the animals were admitted to the hole board for 13 consecutive days with all holes baited). The T group (trained) was subjected to the hole board for 5 consecutive habituation days with all holes baited (similar to the H and P groups), followed by 8 successive training days with only four holes baited in a fixed pattern. During the 8 training days, the T group gradually acquired a pattern to visit the baited holes, whereas the P group continued visiting holes in a random fashion. The mice were killed 24 h after the last behavioral session. All principal cells in teh cornu ammonis (CA) and dentate gyrus (DG) of the habituated animals revealed increased levels of mAChR immunoreactivity (mAChR-ir) over the naive mice. A minor increase in mAChR-ir was found in the apical dendrites of the CA1 pyramidal cells. Pseudotraining resulted in a CA1-CA2 region with a low level of mAChR-ir, resembling naive animals, whereas the trained mice showed a further increase in mAChR-ir in the CA1-CA2 pyramidal cell bodies and apical dendrites. Optical density measures of the mAChR-ir in the CA1 region revealed a significant (P < 0.05) increase in the pyramidal cell bodies of the H and T group over the N and P group, and a significant (P < 0.05) increase in the apical dendrites of the T group over all other groups. In contrast to the CA1-CA2 region, both pseudotrained and trained mice revealed high mAChR staining in the CA3-CA4 region and the DG. These results indicate that prolonged exposure to the hole board is sufficient for an enhanced mAChR-ir in the CA3-CA4 and DG, whereas the increase in CA1-CA2 pyramidal cells is a training-specific feature related to spatial orientation. Nonpyramidal neurons within the CA1-CA2 region with enhanced mAChR-ir in the pyramidal cells, however, revealed a decreased level of mAChR-ir. The opposing effect of pyramidal and nonpyramidal cells suggests a shift in the excitability of the hippocampal microcircuitry. Previously we demonstrated an increase and redistribution of hippocampal protein kinase C gamma-immunoreactivity (PKC gamma-ir) induced by hole board learning in mice (Van der Zee et al., 1992, J Neurosci 12:4808-4815). Immunofluorescence double-labeling experiments conducted in the present study in naive and trained animals revealed that the principal cells and DG interneurons co-express mAChRs and PKC gamma, and that the immunoreactivity for both markers increased in relation to spatial orientation within these neurons. The mAChR-positive nonpyramidal cells of the CA1-CA2 region were devoid of PKC gamma and revealed an opposite training-induced effect. These results suggest that the postsynaptic changes in mAChR- and PKC gamma-ir reflect functional alterations of the hippocampal formation induced by spatial learning.

Sex differences in fear reactions in sheep

In order to assess potential sex differences in fear reactions, 40 rams and 40 ewes were studied using a battery of tests previously designed and validated. Animals were individually confronted with situations classically reported to induce fear in sheep: i.e. isolation from conspecifics, surprise effect and presence of a human. Eight groups were studied, each comprising ten animals of the same sex, breed (Romanov or Ile de-France) and rearing condition (artificially or dam-reared). Males and females of the same breed and rearing condition were tested alternately during the same week (4 days of habituation plus 3 days of tests) in order to prevent fear reactions from being biased by differences in the environment (such as noises, ambient light, etc.) which could influence fear reactions. Twenty-six behavioural items related to fear were recorded. Furthermore, an overall score, based upon those items and reflecting the fear level of each subject in each of the test situations, was calculated. All the significant differences between sexes ( P < 0.05) consistently indicate that rams are less fearful than ewes. Their overall scores were respectively, 37.0 ± 10.5 vs. 44.0 ± 11.9 ( P < 0.01) in the isolation test, 36.1 ± 11.0 vs. 44.9 ± 8.4 ( P < 0.001) in the surprise test, 38.5 ± 12.5 vs. 42.5 ± 13.6 ( P = 0.06) in the human test (higher scores reflect heighthened fearfulness). When subjected to non-social fear-eliciting situations, rams were seen to be less fearful than ewes. This conclusion could serve as an impetus for more general behavioural studies of factors influencing fearfulness and thus adaptability of farm animals to intensive housing conditions.

Circadian and Sleep-Related Endocrine Rhythms in Schizophrenia

Plasma levels of prolactin, growth hormone, corticotropin, and cortisol were measured at 15-minute intervals for 24 hours in nine unmedicated male schizophrenic patients and in nine age-matched normal male subjects. Each study was preceded by 3 days of habituation to the laboratory environment. Sleep was polygraphically recorded. The circadian and pulsatile variations present in each hormonal profile were quantitatively characterized with the use of computer algorithms specifically designed for analyses of hormonal fluctuations. The major abnormality of neuroendocrine release that was observed in the schizophrenic patients was an almost threefold enhancement of the sleep-related increase in the prolactin level, associated with an intensified frequency of nocturnal prolactin pulses. This increased stimulatory effect of sleep on prolactin secretion was evident immediately after sleep onset. The normal inhibition of cortisol secretion during early sleep was absent in schizophrenic patients. The major sleep abnormalities were a prolonged sleep latency and a reduction in total rapid eye movement stage sleep. During wakefulness, prolactin and cortisol levels were normal. The 24-hour profile of growth hormone was unaltered in schizophrenic patients, and a sleep-onset growth hormone pulse was observed in all patients. No abnormalities were noted in the levels or temporal organization of corticotropin secretion. Both the amplitude and the timing of the cortisol rhythm were normal. We conclude that, in schizophrenic men, pituitary-adrenal function and circadian time-keeping are normal but prolactin secretion is hyperresponsive to the physiologic stimulus of sleep onset. Schizophrenia thus appears to be characterized by a subset of neuroendocrine disturbances distinct from that observed in major endogenous depression.

Effect of forebrain dopamine depletion on novelty-induced place preference behavior in rats

Novelty-induced place preference behavior of rats was studied in two experiments. In the first experiment, separate groups of animals were habituated to a distinct environment 30 min daily for either zero, one, two, four or eight days. On the day following the last habituation day, animals were allowed 15 min free access to both the habituated (familiar) and a distinct novel environment. The results revealed a significant novelty preference in the two-, four- and eight-day habituation groups. In these same animals, the rate of horizontal and vertical activity was lower in the novel environment relative to the familiar environment. The influence of forebrain dopamine (DA) projections on novelty preference behavior was studied in the second experiment. Animals were given an injection of 6-hydroxydopamine (6-OHDA) into the nucleus accumbens or were given sham surgery, and then they were given four habituation days to one environment. Novelty-induced place preference was blocked in the lesioned animals, as the amount of time spent in the novel and familiar environments was not significantly different. Lesioned animals also failed to show a difference in locomotor activity between the novel and familiar environments. Subsequent assay data revealed that the 6-OHDA lesion reduced DA levels in the nucleus accumbens, anterior striatum and olfactory tubercles by over 65% as compared to sham surgery. These results suggest that novelty preference behavior may be mediated by a central DA pathway similar to that involved in other types of reinforcing stimuli, such as food, water and drugs of abuse.

Effect of habituation to subanesthetic N2 or N2O levels on pressure and anesthesia tolerance.

Exposure of CD-1 mice to subanesthetic partial pressures of N2O (0.5 atm) or N2 (10-20 atm) for periods up to 14 days results in up to 40% decreases in the mean threshold pressure eliciting type I high-pressure neurological syndrome (HPNS) seizures, and in increases up to 38% in the N2 partial pressure producing anesthesia. For all combinations of preexposure time, N2 partial pressure, as well as identity of the conditioning gas the relations between the convulsion threshold pressure (Pc) and the anesthesia N2 pressure (Pa) appear to be uniquely correlated by the equation Pa = 54.5 - 0.2(Pc - 60)1.2. The potency of N2O with respect to these habituation phenomena is between 28 and 33 times higher than that of N2, depending on the aspects compared. Evidence is presented indicating that after 14 days of habituation the animals have attained between 75 and 85% compensation for the anesthetic as well as the anticonvulsant effects of the conditioning gas. The bearing of the results on the problem of the nature of the antagonism between inert gas narcotic agents and high pressure and on the hypothesis that habituation tends toward restoration of isofluidity (or some analogous normalization process) are discussed.

Effects of chronic stress on plasma corticosterone, ACTH and prolactin

Rats were placed in a stressful environment for 24 hr per day and levels of plasma hormones were measured after varying numbers of days in the environment. Rats were habituated to operant chambers placed in sound-attenuated enclosures. Food pellets were available by lever press on a FR1 schedule. After 3 days of habituation, rats in the “stressed” group were trained to pull a ceiling chain to avoid or escape shock. Following training, stress trials, consisting of a consecutive sequence of 5 sec each of a warning light, warning tone and 0.16, 0.32, 0.65, 1.3 and 2.6 mA of footshock, occurred approximately once per 5 min around-the-clock. For the first day, the sequence was terminated when the ceiling chain was pulled. On subsequent days, 90% of all shock presentations could be avoided or escaped by chain pull; the remaining 10% of trials were inescapable and the entire sequence was presented. Control rats lived in identical chambers without presentation of shock. Rats were sacrificed after 1, 2, 3, 4, 7 or 14 days in this environment and levels of plasma corticosterone, ACTH and prolactin were determined. Levels of plasma corticosterone were elevated during the first 7 days in the stressful environment, but returned to control values by day 14. Levels of plasma ACTH and prolactin were similar in stressed and control rats at all time points measured. These data suggest that stress-induced changes in glucocorticoids but not in ACTH or prolactin might mediate some of the physiological changes that occur as the result of chronic stress.

Long term reduction in eight arm maze performance after early exposure to phenobarbital

Performance in the hippocampal eight arm maze was studied in mice after early exposure to phenobarbital (PhB). since previous studies suggested that these animals suffered neural deficits in the hippocampus. For prenatal exposure pregnant mothers were fed 3 g PhB/kg milled food on gestation days 9–18. Neonates were injected daily with 50 mg PhB/kg. on postnatal days 2–21. After a week of water deprivation, the animals were tested at age 50 days for 5 days preceded by 1 day of habituation. Deficits in eight arm maze performance were demonstrated in early treated mice on every testing day. For example, on day 5 of testing the number of correct entries during the first eight attempts in the prenatally treated group were 12% below control level (P<0.01), the respective reduction in the neonatal group was 10% (P< 0.001). The number of trials needed to enter all arms on day 5 was 27% above control level among prenatally treated mice (P< 0.001), and 13% in neonatally treated mice (P< 0.05). It took prenatal PhB animals twice the time to reach criterion than their controls (P< 0.001) and four times as long for neonatally treated mice (P< 0.001).

Effects of confinement stress on episodic secretion of LH in ovariectomized sheep.

Episodic secretion of luteinizing hormone (LH) in ovariectomized ewes was evaluated before and during acute confinement and during several days of habituation to the same confinement. Plasma concentrations of LH were quantified in samples collected at 5-min intervals via indwelling jugular vein cannulae. Peaks in each time series of plasma LH were identified by a computer program (Pulsar) developed by Merriam and Wachter. In the first experiment, 6 ewes which had been familiarized with the sampling environment and procedures were abruptly moved into a confinement chamber midway through a 4- to 5-hour sampling. Compared with preconfinement sampling, average plasma LH concentrations as well as the incidence (average frequency) and amplitude of identified peaks of LH decreased. In the second experiment, 10 ewes which had not previously been sampled were transferred from their home pens directly into the confinement chamber for a 3- to 4-hour period of blood sampling. This procedure was repeated with the same 10 ewes on the following 2 days to assess the effect of habituation. The average frequency and amplitude of identified peaks of LH increased (p less than 0.05) in habituated ewes, but average plasma LH concentrations did not change. In summary, episodic secretion of LH was inhibited by the stress of initial confinement, but several days of habituation to the same daily periods of confinement minimized this inhibition and restored the episodic discharges of LH.

Cross-modality contrast: Exteroceptive context habituation enhances taste neophobia and conditioned taste aversions

The relationship between absolute and relative stimulus novelty was examined within the context of the conditioned taste aversion paradigm in which the relative novelty of the conditioned interoceptive stimulus was manipulated by differential exteroceptive context habituation. Rats received similar isolation histories but either 5 or 30 days of habituation to the test environment prior to treatment. One group was administered lithium chloride following saccharin consumption, a second group was administered isotonic saline following saccharin consumption, and a third group was administered saline after water consumption. The animals habituated for 30 days exhibited greater conditioned avoidance and greater neophobic avoidance of saccharin than did animals habituated for only 5 days. The results are interpreted in terms of a cross-modality stimulus contrast effect which implicates context habituation as an important parameter of both taste neophobia and taste aversion learning.

Electrophysiological correlates of habituation and conditioning.

1. During habituation of the animal to the experimental situation there is a gradual decrease in the mean coefficient of correlation between the EEG of different cortical points, accompanied by a decrease in the degree of representation of frequencies of the theta-band in spectral and coherent functions. This process is observed during the first four days of habituation, after which the electrographic manifestations show no regular changes. 2. For the first few days of conditioning the mean coefficient of correlation between the EEG of different cortical points rises gradually; this is usually followed by a temporary phase of a decrease in the coefficient, accompanied by a simultaneous decrease in the number of correct conditioned responses, after which the level of spatial synchronization of cortical potentials rises again. The increase in the mean coefficient of correlation is usually accompanied by an increase in the degree of representation of theta-frequencies in the spectral and coherent functions and, conversely, during the phase of weakening of conditioned-reflex activity the degree of representation of the theta-frequencies falls.

Effects of neophobia and habituation on the poison-induced avoidance of exteroceptive stimuli in the rat.

Two experiments on the role of neophobia in poison-induced aversions to exteroceptive stimuli are reported. In Experiment 1, rats were given either 10 or 25 days of habituation to the test situation prior to conditioning. Those animals with the longer habituation period avoided a complex of novel exteroceptive stimuli while those with the shorter habituation period did not. In Experiment 2 rats initially avoided the more novel of two containers, but gradually came to eat equal amounts from both. A single pairing of toxicosis with consumption from either the novel or the familiar container reinstated the avoidance of the novel container in both cases. The results were discussed in terms of an interaction between habituation and conditioning procedures. It was suggested that previously reported differences between interoceptive and exteroceptive conditioning effects may have been influenced by the differential novelty of the two classes of stimuli in the test situation. It was further suggested that non-contingently poisoned control groups should routinely be included in poison avoidance conditioning studies.

Deprivation-Feeding Cycle and Locomotor Activity of the Albino Rat in a Complex Maze

Locomotor activity of three male albino rats was measured electromechanieally in a complex maze to investigate the influence of the recording device upon a specific activity of the rats under cyclic food deprivation. After 14 days of habituation to the apparatus and 7 days of baseline level of locomotor activity, Ss were maintained on a 23-hour food deprivation cycle for 7 days. Under deprivation, Ss were fed during one hour at the beginning of the light period in order to discriminate both effects of the onset of darkness and daily feeding. Activity increased markedly the hour preceding daily feeding. But activity over a 24-hour period did not increase, in contrast with other studies on cyclic food deprivation. This discrepancy was attributed to the use, in the present study, of an environment which is conducive to a species-determined behavior.

Periodicity of food intake in the rat as measured by an operant response

Seven rats were employed to study the periodicity of food intake as measured by a simple bar press response. After 14 days of habituation to a CRF schedule in a 12-hr dark, 12-hr light environment, animals' responses were recorded during four subsequent days. Mean number of meals, bar presses/meal, and total number of bar presses were greater during the dark period, whereas intervals between successive meals were significantly shorter than those during comparable light periods. No differences were found between the ratio of meal length to bar presses or the rate of responding per meal during the dark and the light periods. Results were compared with recent reports of feeding patterns in rats allowed free access to food.