Patients diagnosed with preterm premature rupture of membranes (PPROM) are managed with a seven day course of antibiotics. Maternal serum interleukin (IL)-6 levels are reported to identify women with PPROM likely to develop funisitis. The purpose of this study is to determine if maternal serum IL-6 levels remain predictive of funisitis after completion of antibiotic administration in PPROM subjects. This is a secondary analysis of a prospective cohort study. After IRB approval, daily blood samples were obtained from PPROM subjects and analyzed for IL-6 by ELISA. Serum samples collected while subjects were on antibiotics were excluded. Subjects (N=44) were divided into those with and without funisitis. Data were analyzed using Mann Whitney U test. There was no difference in gestational age at admission, race, marital status, insurance, delivery route, or vaginal bleeding in subjects with and without funisitis. Subjects with funisitis had a significantly shorter latency (12.8 vs. 26.1 days, P< 0.03), earlier gestational age at delivery (29.4 vs. 32.1 weeks, P<0.02), and smaller birth weight (1270 vs. 1765 grams, P<0.03). Maternal serum IL-6 levels obtained at 24 to 48 hours and 48 to 72 hours before delivery are elevated in women with PPROM with funisitis compared with those without funisitis (6.3 vs. 2.7 pg/mL, P<0.03 and 6.1 vs. 1.7 pg/mL, P<0.02). Using values obtained 24-72 hours prior to delivery, a receiver operator characteristic curve analysis was performed. Optimizing for sensitivity, a maternal serum IL-6 level of 1.98 pg/mL had a sensitivity of 86.7%, specificity 46.7%, positive predictive value 61.9%, and negative predictive value 77.8%. This data suggest that maternal serum IL-6 levels continue to be predictive of PPROM subjects destined to develop funisitis after the completion of antibiotic administration. IL-6 levels may be useful in guiding clinical management such as resumption of antibiotics which may prolong latency periods and decrease the incidence of funisitis, resulting in improvement of neonatal outcomes.