Jiangmen Underground Neutrino Observatory (JUNO) is constructed to probe multiple physics goals, including determining the neutrino mass ordering (MO), measuring solar neutrinos, detecting supernova neutrinos, etc. A large Liquid Scintillator (LS) detector with a high energy resolution is being built to determine the neutrino MO within 6-year of data taking. To accomplish this goal, a calibration complex has been designed. Concerning the energy leakage near the LS boundary, a Guide Tube Calibration System (GTCS) will be deployed on the outer surface of the JUNO central detector to help better understand the boundary effect. A fitting algorithm has been presented during the design of GTCS considering the overlap of full-energy peak and Compton tail. In this paper, we verify the fitting algorithm experimentally, using a radioactive source in the Daya Bay experiment. The maximum bias of full-energy peak for the experiment at Daya Bay is less than 1.0% even if a change of ±20.0% in acrylic density was assumed. Besides, the tuned acrylic density is applied in JUNO GTCS simulation. The maximum uncertainty estimation of GTCS energy response in total is 0.52%, satisfying the JUNO GTCS requirement which is 1.0%.
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