Days Of Confinement Research Articles

Beef quality of Nellore steers fed dried or rehydrated and ensiled corn or sorghum grains

Background: Rehydration of grains, such as corn and sorghum, is used to increase nutrient absorption. However, the effect of this practice on meat quality is poorly understood. Objective: To evaluate the effects of type of grain and processing on the meat quality of Nellore steers in a feedlot. Methods: Twenty-four non-castrated Nellore steers (270 ± 53 kg initial body weight) were distributed in a completely randomized 2×2 factorial design, with six replicates. The first factor was cereal type (corn or sorghum), and the second was the grain processing (dry or rehydrated and ensiled). The diets were composed of 28.44% corn silage and 71.56% concentrate. Sixty days before the beginning of the experiment, corn and sorghum grains were rehydrated and ensiled. The animals were slaughtered after 140 days of confinement. Meat quality analyses were determined in samples of fresh and aged meat (7 days) from the Longissimus lumborum muscle. Results: no difference between treatments was observed for carcass pH and L* (lightness), a* (redness), and b* (yellowness) values, shear force, thawing and cooking losses, and chemical composition of meat (p>0.05). The b* (yellowness) value of subcutaneous fat was higher in steers fed corn, regardless of grain processing (p=0.03). Sarcomere length was higher in aged meat of steers fed sorghum, regardless of processing method (p=0.01). Conclusions: the grain processing method does not affect beef quality; however, grain type can affect subcutaneous fat color and sarcomere length of aged beef.

47 Interaction of dietary protein and endophyte- infected Tall Fescue seed in stocker steers on finishing phase performance and carcass traits

Abstract Fescue toxicosis is a multifaceted disease that negatively impacts cattle. It was hypothesized that exposure to endophyte-infected fescue during the stocker phase affects finishing phase growth and carcass characteristics. Eight-month-old steers were sorted by weight and randomly assigned to receive one of four treatments for 56 days in confinement: endophyte-free seed (0 ug/kg ergovaline) with either 18% (EF-18; n = 9) or 14% dietary protein (EF-14; n = 9) and endophyte-infected seed (500 ug/kg ergovaline) with 18% (EI-18; n = 9) or 14% dietary protein (EI-14; n = 9). Following fescue exposure calves were backgrounded on novel endophyte fescue pastures for 8 mo. At 18 mo, all steers were vaccinated, implanted, and reentered the feedlot for a 112 d growing phase and 52 d finishing phase. Body weight was recorded every 28 d and carcass data was collected at slaughter. Data were analyzed using the MIXED procedure of SAS for effects of BW, ADG, dressing percentage (DP), HCW, backfat, ribeye area (REA), KPH, USDA Yield grade (YG), and USDA Quality grade (QG). Statistical significance was determined at P > 0.05) HCW, BW, backfat, REA, DP, or QG. TheEI-14 had a greater ADG (1.69 kg/d) during the feedlot growing phase compared to the other treatment groups (1.53, 1.47, 1.55 for EI-18, EF-14, EF-18, respectively; P > 0.05). Steers stockered on 18% dietary protein had greater BW compared to steers previously fed at 14% (501.3±3.8 vs. 489.7±3.8; P = 0.0339). In addition, 18% protein in stocker diet resulted in greater KPH (2.3±0.2 vs. 1.78±0.2; P = 0.0129) and YG (3.4±0.1 vs. 3.0±0.1; P = 0.0168) compared with steers fed 14% dietary protein. Finishing growth and carcass characteristics were not affected by exposure to ergot alkaloids at weaning as much as dietary protein when cattle had a background period prior to feedlot entry

149 Interaction of dietary protein and endophyte- infected Tall Fescue seed in stocker steers on finishing phase performance and carcass traits

Abstract Fescue toxicosis is a multifaceted disease that negatively impacts cattle. It was hypothesized that exposure to endophyte-infected fescue during the stocker phase affects finishing phase growth and carcass characteristics. Eight-month-old steers were sorted by weight and randomly assigned to receive one of four treatments for 56 days in confinement: endophyte-free seed (0 ug/ kg ergovaline) with either 18% (EF-18; n = 9) or 14% dietary protein (EF-14; n = 9) and endophyte-infected seed (500 ug/kg ergovaline) with 18% (EI-18; n = 9) or 14% dietary protein (EI-14; n = 9). Following fescue exposure calves were backgrounded on novel endophyte fescue pastures for 8 mo. At 18 mo, all steers were vaccinated, implanted, and reentered the feedlot for a 112 d growing phase and 52 d finishing phase. Body weight was recorded every 28 d and carcass data was collected at slaughter. Data were analyzed using the MIXED procedure of SAS for effects of BW, ADG, dressing percentage (DP), HCW, backfat, ribeye area (REA), KPH, USDA Yield grade (YG), and USDA Quality grade (QG). Statistical significance was determined at P > 0.05) HCW, BW, backfat, REA, DP, or QG. TheEI-14 had a greater ADG (1.69 kg/d) during the feedlot growing phase compared to the other treatment groups (1.53, 1.47, 1.55 for EI-18, EF-14, EF-18, respectively; P > 0.05). Steers stockered on 18% dietary protein had greater BW compared to steers previously fed at 14% (501.3±3.8 vs. 489.7±3.8; P = 0.0339). In addition, 18% protein in stocker diet resulted in greater KPH (2.3±0.2 vs. 1.78±0.2; P = 0.0129) and YG (3.4±0.1 vs. 3.0±0.1; P = 0.0168) compared with steers fed 14% dietary protein. Finishing growth and carcass characteristics were not affected by exposure to ergot alkaloids at weaning as much as dietary protein when cattle had a background period prior to feedlot entry

PSII-23 Meat quality of Santa Inês lambs slaughtered at different backfat thickness

Abstract The objective of this study was to evaluate the performance and meat characteristics of castrated and intact Santa Inês male lambs slaughtered at three different thicknesses of subcutaneous fat. Thirty-four lambs, approximately 3 months of age, 19.37 ± 3.73 kg of body weight were used. Four weeks prior to the experiment, half of the animals were castrated. Lambs were further divided into three groups, according to the criteria for slaughter: fat thicknesses of 1, 3 and 6 mm; measured with an ultrasound equipment. All animals received the same diet. Criteria of 1 and 3 mm were achieved after 39 and 84 days in feedlot. As the criterion of 6 mm was not attained up to 130 days of confinement, lambs were slaughtered at that time, with 4 mm of fat thickness. Fat thickness measured with a caliper on the carcasses were 0.87, 3.11 and 3.60 mm. There were no interactions (P > 0.05) between sexual conditions and slaughter criteria. Average daily gain was similar (P > 0.05). Among criteria (average of 0.267 ± 0.052 kg/day). However, final body weight (29.94 ± 2.03; 40.42 ± 2.67 and 53.85 ± 2.03 kg) and feed conversion (4.17 ± 0.39; 4.71 ± 0.43 and 6.01 ± 0.39) were different (P0.05) among criteria, panelists found that lambs slaughtered with 3.6 mm of fat thickness were less (P < 0.05) desirable. Average daily gain (0.283 ± 0.013 vs 0.247 ± 0.012 kg) was greater (P < 0.05) for intact than castrated lambs; however, the meat of intact lambs was less desirable. The other traits were similar for both sexual conditions. We can conclude by the performance and meat results that it is better to slaughter Santa Inês lambs with 3 mm of fat cover, and for the best acceptability of the meat the lambs should be castrated.

Living In Confinement Conditions: Physical Training Can Improve Muscular Oxygen Uptake and Heart Rate Kinetics

PURPOSE: Inside the NASA Human Exploration Research Analog (HERA) facility the effects of 45 d of confinement in combination with restrictions for sleep and a defined physical training on muscular oxygen uptake (V’O2musc) kinetics and cardiovascular regulation during exercise was investigated. METHODS: To date, fourteen healthy individuals (5 females, 9 males, 37±7 y, 23±3 kg∙m-2) were analyzed 8 d before (MD-8), during and 4 d after (MD+4) a simulated Space mission. A cycle exercise test with pseudo-random binary work rate changes (WR) of 30 W and 80 W and an incrementally increasing step protocol (25 W min-1) to assess peak oxygen uptake (V’O2peak) was applied. Heart rate (HR) and mean arterial blood pressure (MAP) were measured beat-to-beat and pulmonary oxygen uptake (V’O2pulm) breath-by-breath. V’O2musc was estimated from HR and V’O2pulm. Kinetics responses were assessed by maxima of the cross correlation function (CCFmax) between WR and the respective parameter indicate faster kinetics (Hoffmann et al., Eur J Appl Physiol 113:1745-1754, 2013). During the mission, exercise training sessions were scheduled every second day with a maximal HR restricted to below 85% of the age-related maximum. Sleep was restricted to 5 h per weekday and 8 h at the weekends. Differences in V’O2peak and kinetics from MD-8 to MD+4 were calculated and correlated with the values measured at MD-8 using the Pearson test. Level of significance was set to α = 5%. RESULTS: V'O2peak differed not significantly (P=0.221) between MD-8 (37.8±5.8 ml min-1 kg-1) and MD+4 (38.9±4.6 ml min-1 kg-1). Changes in CCFmax(HR) correlated significantly with CCFmax(HR) at MD-8 (r = -0.839, P < 0.001), changes in CCFmax(V’O2musc) correlated significantly with CCFmax(V’O2musc) at MD-8 (r = -0.641; P = 0.014) and the difference in V’O2peak correlated with V’O2peak at MD-8 (r = -0.614; P = 0.019). CONCLUSIONS: Exercise training during forty-five days of confinement in combination with sleep restrictions, may prevent from losses in cardio-muscular kinetics. Those individuals who started with slow kinetics or a low V’O2peak benefited from the exercise training during the mission. The volume and/or intensity of the exercise training intervention might have been higher during the HERA C4 missions compared to most of the crew members’ everyday life activities.

Favorable Changes in Biomarkers of Potential Harm to Reduce the Adverse Health Effects of Smoking in Smokers Switching to the Menthol Tobacco Heating System 2.2 for 3 Months (Part 2).

IntroductionTobacco Heating System (THS) 2.2, a candidate modified-risk tobacco product, aims at offering an alternative to cigarettes for smokers while substantially reducing the exposure to harmful and potentially harmful constituents found in cigarette smoke.MethodsOne hundred and sixty healthy adult US smokers participated in this randomized, three-arm parallel group, controlled clinical study. Subjects were randomized in a 2:1:1 ratio to menthol Tobacco Heating System 2.2 (mTHS), menthol cigarette, or smoking abstinence for 5 days in confinement and 86 subsequent ambulatory days. Endpoints included biomarkers of exposure to harmful and potentially harmful constituents (reported in our co-publication, Part 1) and biomarkers of potential harm (BOPH).ResultsCompliance (protocol and allocated product exposure) was 51% and 18% in the mTHS and smoking abstinence arms, respectively, on day 90. Nonetheless, favorable changes in BOPHs of lipid metabolism (total cholesterol and high- and low-density cholesterol), endothelial dysfunction (soluble intercellular adhesion molecule-1), oxidative stress (8-epi-prostaglandin F2α), and cardiovascular risk factors (eg, high-sensitivity C-reactive protein) were observed in the mTHS group. Favorable effects in other BOPHs, including ones related to platelet activation (11-dehydrothromboxane B2) and metabolic syndrome (glucose), were more pronounced in normal weight subjects.ConclusionsThe results suggest that the reduced exposure demonstrated when switching to mTHS is associated with overall improvements in BOPHs, which are indicative of pathomechanistic pathways underlying the development of smoking-related diseases, with some stronger effects in normal weight subjects.ImplicationsSwitching to mTHS was associated with favorable changes for some BOPHs indicative of biological pathway alterations (eg, oxidative stress and endothelial dysfunction). The results suggest that switching to mTHS has the potential to reduce the adverse health effects of smoking and ultimately the risk of smoking-related diseases. Switching to mTHS for 90 days led to reductions in a number of biomarkers of exposure in smokers, relative to those who continued smoking cigarettes, which were close to those observed when stopping smoking (reported in our co-publication, Part 1). Initial findings suggest reduced levels of 8-epi-prostaglandin F2α and intercellular adhesion molecule 1, when switching to mTHS for 90 days. These changes are comparable to what is observed upon smoking cessation. In normal weight subjects, additional favorable changes were seen in 11-dehydrothromboxane B2, fibrinogen, homocysteine, hs-CRP, percentage of predicted forced expiratory volume in 1 second, systolic blood pressure, diastolic blood pressure, glucose, high-density lipoprotein, apolipoprotein A1, and triglycerides.Trial registrationNCT01989156.

A Meta-Analysis of the Performance of a Blood-Based Exposure Response Gene Signature Across Clinical Studies on the Tobacco Heating System 2.2 (THS 2.2).

As part of emerging tobacco harm reduction strategies, modified risk tobacco products (MRTP) are being developed to offer alternatives that have the potential to reduce the individual risk and population harm compared with smoking cigarettes for adult smokers who want to continue using tobacco and nicotine products. MRTPs are defined as any tobacco products that are distributed for use to reduce harm or the risk of tobacco-related disease associated with commercially marketed tobacco products. One such candidate MRTP is the Tobacco Heating System (THS) 2.2, which does not burn tobacco but instead heats it, thus producing significantly reduced levels of harmful and potentially harmful constituents compared with cigarettes. The clinical assessment of candidate MRTPs requires the development of exposure-response markers to distinguish current smokers from either nonsmokers or former smokers with high specificity and sensitivity. Toward this end, a whole blood-derived gene signature was previously developed and reported. Four randomized, controlled, open-label, three-arm parallel group reduced exposure clinical studies have been conducted with subjects randomized to three arms: switching from cigarettes to THS 2.2, continuous use of cigarettes, or smoking abstinence. These clinical studies had an investigational period of 5 days in confinement, which was followed by an 85-day ambulatory period in two studies. Here we tested the previously developed blood-derived signature on the samples derived from those clinical studies. We showed that in all four studies, the signature scores were reduced consistently in subjects who either stopped smoking or switched to THS 2.2 compared with subjects who continued smoking cigarettes.

Carcass characteristics of lambs fed diets with increasing levels of crude glycerin.

ObjectiveAn experiment was conducted to evaluate the effects of increasing levels of crude glycerin (0%, 6%, 12%, and 18%) used as a substitute for corn in lamb feed on the quantitative characteristics of the carcass.MethodsA total of 40 crossbred Santa Inês lambs that were four months old with a mean initial weight of 21.0±0.8 kg were randomly distributed in four treatments with ten replicates. The animals were slaughtered after 66 days of confinement. The effects of crude glycerin as a replacement for corn in the diet of the lambs on the carcass characteristics, commercial cut weight and yield and carcass measurements were studied.ResultsThere was an increasing linear effect for body weight at slaughter with the replacement of corn by crude glycerin. The dry matter and metabolizable energy intakes, weight of the empty body, hot carcass weight and cold carcass weight showed a quadratic effect, with maximum crude glycerin levels estimated at 10.9%, 9.8%, 10.83%, 11.78%, and 11.35%, respectively. The initial pH was not influenced by the replacement of corn for crude glycerin, while the final pH presented a quadratic effect. The other parameters of the carcass and the weights and yields of commercial cuts were not influenced. There was also no effect of the diets on carcass morphometric measurements, except for the thoracic perimeter and the carcass compactness index, which presented quadratic and linear effects, respectively.ConclusionCrude glycerin can replace up to 18% of corn because it favours muscle tissue deposition without promoting changes in the main carcass characteristics of lambs.

Morphometric measurements of sheep fed with increasing levels of sunflower meal

The objective of this work was to evaluate the performance, body morphometric measurements and testicular development in lambs fed with different rates of sunflower meal inclusion. The animals (24) were randomly distributed in treatments with 0, 10, 20 and 30% of sunflower meal in the dry matter of the diet, in six replicates, during 56 days of confinement. The addition of the coproduct at increasing dietary rates increased linearly the dry matter intake in the percentage of live weight and neutral detergent fiber. Despite this, there was a linear reduction in ether extract intake and chest width. On the other hand, there was no difference in daily, total and final weight gains. The body condition score; height of withers, croup and thorax; length of body and croup; width of fore croup, hind croup and chest; thoracic and neck diameters also did not change with the addition of the coproduct. The testicular measures 26.76; 6.11; 6.08; 5.22; 5.21; 4.80, and 4.81cm varied quadratically with the inclusion. Therefore, the inclusion of 30% of the coproduct changes nutrient intake and testicular biometry, but does not interfere in the body weight and development, being an economical alternative.

Physicochemical composition and fatty acids profile of the meat of lambs fed with cassava wastewater

The effects of replacing corn with cassava wastewater at 0; 25; 50; 75 and 100% in the sheep diet over chemical composition, fatty acid profile and physical-chemical parameters of meat were studied. Forty Santa Inês breed sheep were slaughtered after 70 days of confinement. The moisture content of meat increased, while the crude protein and ether extract decreased linearly with the replacement. There was a quadratic effect for shear force with minimum force estimated at 2.78 kg / cm ² with 54.25% replacement. There was a quadratic effect for C15:0 and C:17, (0.37 and 1.30 when the replacement was 46.25% and 68.50%, respectively), increasing linearly for the C18:0 and decreasing for the C22:0. For monounsaturated fatty acids there was a decreasing linear effect for C14:1 and C20:1 n9, and increasing to C18:1 n11. For C15:1 a quadratic effect was observed (1.48 when 26.75% of corn was replaced). As to the polyunsaturated fatty acids, there was a quadratic effect for C18:2 9c 11t (0.60 when replacement was 36.66%). There was a linear decrease for relations polyunsaturated: saturated and ω6: ω3, without effect on other relations. Cassava wastewater alters the shear force and chemical and lipid composition of meat.

Blood parameter of Nellore steers submitted to different levels of feed intake

Blood parameters of 18 Nellore steers submitted to high concentrate diet in two sequential feeding regimes (Phase 1: restriction and Phase 2: ad libitum feed) were evaluated. In the first phase, steers were fed during 56 days in three intake levels of dry matter (ad libitum, 75 g dry matter (DM)/kg metabolic body weight (BW0.75) or 60 g DM/kg BW0.75. In the second phase, from the 57th day of confinement, animals fed 60 g DM/kg BW0.75 and 75 g DM/kg BW0.75 received feed ad libitum until the 136th day. In the 8th, 27th, 55th (first phase), 71st, 84th, 112th and 132nd (second phase day of experimental period, blood samples were collected and insulin-like growth factor I (IGF-I), triiodothyronine (T3), thyroxine (T4), glucose, plasma ureic nitrogen (PUN) and total protein (TP) were determined. In the first phase IGF-I, T4, glucose and total protein concentration was not different among treatments (P>0.05) and there was no interaction between treatment and time (P>0.05). There was an interaction among treatments and time on T3 and plasma ureic nitrogen (P 0.05). For plasma ureic nitrogen concentration there was an interaction among treatments and time (P=0.02). Lower levels of diet intake can be used to improve animal performance in feedlot.

Performance and carcass characteristics of feedlot-finished Zebu cattle in different feeding systems

The performance and characteristics of 18 carcasses of feedlot-finished Zebu cattle were evaluated over 93 days in confinement or semi-confinement systems. In the semi-confinement system, two strategies of pasture deferral were adopted, 40 days (40DD) and 80 days (80DD). The feed of animals in confinement was composed of 24% of grass silage Urochloa spp. and 76% concentrate, on dry matter, freely supplied twice daily. Grazing animals received 4 kg of animal concentrate per day. The final fasting weight of the animals in confinement (534.92 kg) was higher than that of animals semi-confined in 40DD (475.33 kg) and 80DD (460.67 kg) pastures, because of the higher daily weight gain (1.37 vs. 0.82 and 0.64 kg, respectively). Feedlot-finished animals had a greater carcass gain (0.91 kg animal-1 day-1), resulting in a higher carcass weight (296.47 kg), higher thickness of external fat (5.42 mm), weight and percentage of side cut (17.88 kg and 12.20%), and weight and percentage of fat in the carcass (74.50 kg and 25.22%). However, no difference was observed for the metric and developmental characteristics of the carcass, which allowed good quality carcasses to be obtained, independent of the strategies evaluated. Feedlot-finished Zebu cattle provide heavier and bulky carcasses, leading to higher revenue per marketed carcass.

Quality of meat from sheep fed diets containing spineless cactus (Nopalea cochenillifera Salm Dyck)

It was aimed to evaluate the effects of replacement of wheat bran in lambs' diets with spineless cactus (0, 33, 66, and 100%) in a lamb's diet on the physicochemical parameters, fatty acids and sensory analyasis of meat. The evaluation utilized 36 loins and legs from F1 ½ Dorper × ½ Santa Inês lambs, with an average initial body weight of 19.5 ± 1.6 kg, were slaughtered after 70 days of confinement. Were found average values of 5.70, 11.27 N, 55.44 mg/100 g, of meat pH, shear force, and cholesterol, respectively. However, stearic acid, linoleic, linolenic, eicosatrienoic and eicosapentaenoic which linearly decreased. The color, aroma, texture, flavor, juiciness and global acceptance of meat were acceptable by the evaluators, additionally, they said they would buy meat coming of animals fed with 100% replacement. It is recommended that wheat bran is replaced by spineless cactus in the diet of lambs when this shows to be an ingredient with better cost-benefit ratio compared to wheat bran.

Body composition and metabolic changes during a 520-day mission simulation to Mars

The "Mars-500 project" allowed to evaluate the changes in psychological/physiological adaptation over a prolonged confinement, in order to gather information for future missions. Here, we evaluated the impact of confinement and isolation on body composition, glucose metabolism/insulin resistance and adipokine levels. The "Mars-500 project" consisted of 520 consecutive days of confinement from June 3, 2010 to Nov 4, 2011. The crew was composed of six male subjects (three Russians, two Europeans, and one Chinese) with a median age of 31 years (range 27-38 years). During the 520-day confinement, total body mass and BMI progressively decreased, reaching a significant difference at the end (417days) of the observation period (- 9.2 and - 5.5%, respectively). Fat mass remained unchanged. A progressive and significant increase of fasting plasma glucose was observed between 249 and 417 days (+ 10/+ 17% vs baseline), with a further increase at the end of confinement (up to + 30%). Median plasma insulin showed a non-significant early increment (60 days; + 86%). Total adiponectin halved (- 47%) 60 days after hatch closure, remaining at this nadir (- 51%) level for a further 60 days. High molecular weight adiponectin remained significantly lower from 60 to 168 days. Based on these data, countermeasures may be envisioned to balance the potentially harmful effects of prolonged confinement, including a better exercise program, with accurate monitoring of (1) the individual activity and (2) the relationship between body composition and metabolic derangement.

Leg tissue composition and physico-chemical parameters of sheep meat fed annatto coproduct

Our objective was to evaluate leg tissue composition and physico-chemical quality parameters of sheep meat fed with increasing levels of annatto coproduct. 32 male uncastrated animals without a defined breed were randomized in four treatments (0, 100, 200 and 300 g kg-1 of annatto coproduct in the DM diet). After 78 days of confinement, the animals were slaughtered and body components were recorded. Reconstituted leg weight, total muscle weight, biceps weight and semitendinosus weight showed a negative linear behavior (P &lt; 0.05) with the inclusion of the annatto coproduct in the diet. No effects of the inclusion of annatto coproduct (P &gt; 0.05) were found for leg tissue composition (%), muscle:bone ratio, relative fat or leg muscle. Meat physico-chemical parameters (color, shear force, water retention capacity and cooking losses) were not affected by the inclusion of the annatto coproduct in the diet. The annatto coproduct can be included in up to 300 g kg-1 of dietary dry matter without negative effects to the leg tissue composition (%) and physical parameters of confined sheep meat.

Carcass characteristics of lambs fed spineless cactus as a replacement for sugarcane.

ObjectiveFresh sugarcane has been a new roughage source for ruminant’s in semiarid regions, a function of the decline of sugar and alcohol industry in recent years. However, there is little data published regarding lambs fed sugarcane associated with spineless cactus. This study evaluated the effect of sugarcane replacement with spineless cactus (0%, 33%, 66%, and 100%) in the diet of Santa Inês lambs on carcass characteristics.MethodsThirty-six non-castrated Santa Ines lambs at four months of age and an initial body weight of 22±2.3 kg were assigned in a randomized block design and slaughtered after 70 days of confinement. The effects of spineless cactus as a replacement for sugarcane in the diet of the lambs on the carcass characteristics, commercial cut weight and yield, leg tissue composition, and carcass measurements were studied.ResultsThe study revealed quadratic behavior in slaughter body weight, and hot and cold carcass weight, with maximum values of 38.60, 18.60, and 18.11 kg and replacement levels of 40.18%, 44.42%, and 43.14%, respectively. The cold carcass yield presented an increasing linear behavior. The compactness index of carcass and leg presented a quadratic effect, with estimated maximal values of 0.28 and 0.57 kg/cm and replacement levels of 43.37% and 45.5%, respectively. The weights of commercial cuts of leg, loin, shoulder, and breast showed quadratic behavior, with maximum values of 2.79, 0.852, 1.46, and 1.30 kg and replacement levels of 49.5, 45.32, 39.0, and 40.7, respectively. For tissue composition, quadratic behavior was verified for leg weight, subcutaneous fat, and total fat.ConclusionThe replacement of sugarcane by spineless cactus at level 44% is recommended for finishing lambs considering that this level improved most of the carcass characteristics, weights, and yields of commercial cuts and leg tissue composition.

Effects of Switching to the Tobacco Heating System 2.2 Menthol, Smoking Abstinence, or Continued Cigarette Smoking on Biomarkers of Exposure: A Randomized, Controlled, Open-Label, Multicenter Study in Sequential Confinement and Ambulatory Settings (Part 1).

IntroductionThe menthol Tobacco Heating System 2.2 (mTHS) is a newly developed candidate modified-risk tobacco product intended to reduce exposure to the harmful and potentially harmful constituents (HPHCs) of conventional cigarette (CC) smoke. This study examined the impact of switching to mTHS on biomarkers of exposure to HPHCs relative to menthol CCs (mCCs) and smoking abstinence (SA).MethodsIn this three-arm, parallel-group study, 160 Japanese adult smokers (23–65 years; smoking ≥10 mCCs per day) were randomized to mTHS (n = 78), mCC (n = 42), or SA (n = 40) for 5 days in confinement and 85 days in ambulatory settings. Endpoints included biomarkers of exposure to HPHCs, human puffing topography, safety, and subjective effects of smoking measures.ResultsAfter 5 days of product use, the concentrations of carboxyhemoglobin, 3-hydroxypropylmercapturic acid, monohydroxybutenyl mercapturic acid, and S-phenylmercapturic acid were 55%, 49%, 87%, and 89% lower (p < .001), respectively, in the mTHS group than in the mCC group. Other biomarkers of exposure (measured as secondary endpoints) were 50%–94% lower in the mTHS group than in the mCC group on day 5. These reductions in the mTHS group were maintained at day 90, similar to the SA group. Switching to mTHS was associated with changes in human puffing topography (shorter puff intervals and more frequent puffs). The urge-to-smoke and smoking satisfaction levels on day 90 were similar in the mTHS and the mCC groups.ConclusionSwitching from mCCs to mTHS significantly reduced exposure to HPHCs relative to continuing smoking mCCs with concentrations similar to those observed following SA in Japanese adult smokers.ImplicationsThis randomized study compared the impact of switching to a modified-risk tobacco product candidate mTHS on biomarkers of exposure to HPHCs of cigarette smoke relative to continuing smoking cigarettes or abstaining from smoking in sequential confinement and ambulatory settings. The study showed that switching to mTHS was associated with significant biomarker reductions within 5 days in confinement, these reductions being maintained throughout the ambulatory setting up to day 90. The results provide evidence that switching to mTHS reduces real-life exposure to HPHCs in adult smokers.

Effects of Switching to the Menthol Tobacco Heating System 2.2, Smoking Abstinence, or Continued Cigarette Smoking on Clinically Relevant Risk Markers: A Randomized, Controlled, Open-Label, Multicenter Study in Sequential Confinement and Ambulatory Settings (Part 2).

IntroductionModified-risk tobacco products are expected to reduce exposure to harmful and potentially harmful constituents of cigarette smoke, and ultimately reduce the health burden of smoking-related diseases. Clinically relevant risk markers of smoking-related diseases inform about the risk profile of new tobacco products in the absence of in-market epidemiological data. The menthol Tobacco Heating System 2.2 (mTHS) is a modified-risk tobacco product in development as an alternative to cigarettes (conventional cigarettes [CCs]).MethodsIn this parallel-group study, Japanese adult smokers (23–65 years; ≥10 mCCs/day) were randomized to mTHS, menthol CCs (mCC), or smoking abstinence (SA) for 5 days in confinement and 85 days in ambulatory settings. Endpoints included biomarkers of exposure to harmful and potentially harmful constituents and clinically relevant risk markers of smoking-related diseases.ResultsOne-hundred and sixty participants were randomized to the mTHS (n = 78), mCC (n = 42), and SA (n = 40) groups. Switching to the mTHS was associated with reductions in biomarkers of exposure compared with continuing mCCs. Reductions in 8-epi-prostaglandin F2α (biomarker of oxidative stress), 11-dehydro-thromboxane B2 (biomarker of platelet activation), soluble intracellular adhesion molecule-1 (biomarker of endothelial function), and an increase in high-density lipoprotein cholesterol (biomarker of lipid metabolism) and forced expiratory volume in 1 second (biomarker of lung function) occurred in the mTHS group compared with the mCC group. The changes in the mTHS group approached those in the SA group.ConclusionsSwitching from mCCs to mTHS was associated with improvements in clinically relevant risk markers linked to mechanistic pathways involved in smoking-related diseases.ImplicationsIn this three-way randomized study, switching from menthol cigarettes to mTHS for 5 days in confinement and 85 days in ambulatory settings was associated with reductions in biomarkers of exposure to cigarette smoke, and changes were observed in clinically relevant biomarkers of oxidative stress (8-epi-prostaglandin F2α), platelet activity (11-dehydro-thromboxane B2), endothelial function (soluble intracellular adhesion molecule-1), lipid metabolism (high-density lipoprotein cholesterol) and lung function (forced expiratory volume in 1 second), similar to the SA group. The results suggest that switching to the mTHS has the potential to reduce the adverse health effects of conventional cigarettes.

Silagem de bagaço de laranja na alimentação de cordeiros: consumo de nutrientes, desempenho e avaliação econômica

Avaliou-se a inclusão da silagem de bagaço de laranja pré-seco (SBL) em substituição ao milho na dieta de cordeiros no consumo de nutriente, desempenho e custo benefício das dietas. Foram utilizados 20 cordeiros com peso corporal (PC) médio inicial de 25,37 ± 1,94kg, estes foram confinados até que atingissem 35 kg, suas dietas possuíram níveis crescentes de SBL em substituição ao milho (0, 33, 66 e 100%). A ingestão de fibra em detergente ácido não foi influenciada (p&gt;0,05) pela inclusão da SBL, já a ingestão de matéria seca (MS), matéria orgânica, proteína bruta, fibra em detergente neutro e energia metabolizável (Mcal/kg MS) reduziram linearmente (p0,05) em função dos níveis de substituição do milho pela SBL. Já o ganho de peso diário reduziu com a inclusão da SBL, efeito contrário foi observado para os dias de confinamento. O custo total foi maior para o tratamento com 100% de SBL. A margem líquida deste foi a única negativa. Contrariamente o tratamento com 100% de farelo de milho exibiu a maior margem líquida, seguido do nível de 66% de substituição. O custo com a mão de obra foi responsável por mais de 60% do custo total, chegando a 71,16% no tratamento com 100% de SBL. O tratamento sem SBL mostrou-se o mais rentável entre os níveis de substituição pelo subproduto.

Cattle productive performance evaluation confined submitted immunocastration

In order to evaluate the performance and carcass characteristics of cattle cross breeds ½ Aberdeen Angus x ½Nelore and Nelore confined submitted to immunocastration 218 male animals were used, feedlot, averaging 342 kg, divided into three experimental groups, T1: 117 steers ½ Angus x ½ Nelore no castrated (ANC), T2: 51 Nelore steers uncastrated (NNC) and T3: 50 Nellore steers immunocastrated (NIC). The experiment lasted 144 days of confinement. The selection of animals for group formation was according to the individual weight, breed, sex condition and age. For immunocastration it wasused Bopriva® vaccine. The rating was finished according to the parameter used by the meatpacking industry ranging from 1 to 5. The experimental design was completely randomized in three groups. For the analyzes the variables studied statistics were submitted to analysis of variance (ANOVA) and Tukey test both at the 5% level of significance. The results showed differences (p <0.01) at various features of productive performance and carcass between treatments. For slaughter weight, the ANC animals were higher (with 582.1 kg) to Nelore, regardless of sexual condition, and the NNC were in turn heavier than the NIC, 527.4 and 503.7 respectively. Finally, it observed that the use of immunocastration in Nellore animals provided a decrease in productive performance of confined animals, but provided better finish carcass similar to crossbred (ANC).