Datura Plants Research Articles

Phytochemical Profiling and Nutraceutical Benefits of Methanol Leaf Extracts from Datura alba ness

Introduction: Species of the Datura plant have demonstrated significant pharmacological benefits in combating various diseases over the past decade. However, little information is available on Datura alba ness (DAN), and no studies have yet reported its proximate composition or characterized its phytochemical components. This study aims to investigate the nutritional, phytochemical and bioactive properties of Datura alba ness. Materials and Methods: Fresh leaves of Datura alba ness were collected from Abua Odua local government area of Rivers State, Nigeria and properly identified and authenticated. The fresh leaves were subjected to proximate analysis using standard protocols while the dried leaves were extracted using methanol by the method of cold maceration to obtain methanolic extract of Datura alba ness (MEDAN). The MEDAN was further subjected to phytochemical screening to obtain the various phytochemicals and Gas chromatography and mass spectrometry (GC-MS) analysis for detailed identification and characterization of the various bioactive compounds. Results: Proximate analysis of fresh leaves of DAN showed the presence of moisture (60.4%), crude protein (21.9%), carbohydrate (7.6%), crude fibre (4.77%), ash (4.17%) and fat (1.19%) while the phytochemical screening revealed the presence of flavonoids (49.14%), alkaloids (19.16%), tannins 1(12.19%), saponins (11.72%), phenols (5.33%), cardiac glycosides (1.91%), oxalates (0.43%) and phytates (0.43%). Also, the GC-MS analysis of MEDAN identified twenty-nine (29) active compounds with four (4) observed as the most abundant (with area concentration >10%). They include Bis (2-ethylhexyl) phthalate (15.97%), 13-hexyloxacyclotridec-10-en-2-one (14.26%), n-hexadecanoic acid (12.87%) and Gamma.-Sitosterol (10.77). Conclusion: The identification of various nutrients, compounds, and bioactive substances through proximate, phytochemical, and GC-MS analysis of fresh leaves and methanol extract of Datura alba ness highlights its potential as a supplement and therapeutic agent. These findings suggest that Datura alba ness contains numerous bioactive compounds that may offer effective treatments for conditions related to oxidative stress, nervous system disorders, tissue damage, inflammation, and bacterial infections.

Healing and Hallucinations: A Comprehensive Look at Datura

The Solanaceae plant family is a large clade that includes Datura, a genus of plants with many species recognized for their severe pharmacological effects via tropane alkaloids like atropine, hyoscyamine and scopolamine. These alkaloids act as antagonists of muscarinic acetylcholine receptors with leading to a truly diverse pharmacological action, ranging from anticholinergic effects all the way through hallucinogenic properties. Datura plants have been used historically in traditional medicine and rituals worldwide but are infamous for poisoning due to the risk of severe anticholinergic toxicity, including death. Nevertheless, their pharmacology has become of interest for research due to potential therapeutic applications: specifically in controlling nauseousness and perhaps neurological disorders. In this review, we provide a summary of the botanical description and cultivation methods in addition to those on traditional use practices concerning phytochemistry, pharmacology and toxicology as compared with evidence-based information together emphasizing cautions against availability without stringent regulatory consideration for its modern medical uses or ritualistic purposes.

Dying of thirst: Osmoregulation by a hawkmoth pollinator in response to variability in ambient humidity and nectar availability

Climate-induced shifts in flowering phenology can disrupt pollinator-floral resource synchrony, especially in desert ecosystems where rainfall dictates both. However, baseline metrics to gauge pollinator health in the wild amidst rapid climate change are lacking. Our laboratory-based study establishes a baseline for pollinator physiological state by exploring how osmotic conditions influence survivorship in a desert hawkmoth pollinator, Manduca sexta. We sampled hemolymph osmolality from over 1000 lab-grown moths at 20 %, 50 %, and 80 % ambient humidity levels. Starved moths maintained healthy osmolality of 350–400 mmol/kg for 1–3 days after eclosion regardless of ambient humidity, but it sharply rose to 550 mmol/kg after 4–5 days in low and moderate humidity, and after 5 days in high humidity. Starved moths in low humidity conditions perished within 5 days, while those in high humidity survived twice as long. Moths fed synthetic Datura wrightii nectar, synthetic Agave palmeri nectar, or water, maintained osmolality within a healthy range of 350–400mmol/kg. The same was true for moths fed authentic floral nectars from Datura and Agave plants, although moths consumed more synthetic than authentic nectars, possibly due to non-sugar constituents. Simulating a 4-day mismatch between pollinator emergence and nectar availability, a single nectar meal osmotically rescued moths under dry ambient conditions. Our findings highlight hemolymph osmolality as a rapid and accurate biomarker distinguishing dehydrated from hydrated states in insect pollinators.

Cytotoxic and Genotoxic Potentials of Datura innoxia Leaves Aqueous Extract on Allium cepa Meristemic Cells

Medicinal plants are still vitally important in the preparation of pharmaceutical products. Plants have been used both for their prophylactic effects and for the treatment of illnesses and diseases. According to the World Health Organization (WHO) about 80% of developing countries use traditional folk medicines, 85% of which are plant extracts. Datura plant was used medicinally over the world in historical times for treatment of colds, nervous conditions, and as ointment for cuts or wounds and to relieve pain during the setting of bones. The present study aimed to investigate the mutagenic potential of Datura innoxia water extract using Allium cepa root tip cells as biological systems they were used to find out the effect of Datura extract on chromosomal structure and behavior during cell division. Therefore, the mitotic index and chromosome aberration in treated cells were recorded. The root meristem cells were treated with different concentrations of D. innoxia at different times, cytological analysis revealed significant depression in cell division, this was seen noticeably in all used concentrations especially at high ones. The interaction of D. innoxia extract with the genetic material led to DNA damage and decreased cell number depending concentration and time.

Passionfruit severe leaf distortion virus: Expanded host range, response ofPassifloraspp. plants to infection and transmission byBemisia tabaciMEAM1

AbstractPassionfruit severe leaf distortion virus (PSLDV) is a begomovirus transmitted byBemisia tabaciMiddle East‐Asia Minor 1 (MEAM1), associated with severe disease in passion flowers. This study aimed to evaluate the reaction of plants of different species ofPassiflorato infection with PSLDV, expand the known host range of the virus and determine the minimum virus acquisition and inoculation access periods and the time of virus retention byB. tabaciMEAM1. Plants ofPassiflora cincinnata,P. mucronataandP. malacophyllawere not infected with PSLDV through transmission withB. tabaciMEAM1. When healthy plants of these species were fork grafted onto PSLDV‐infected plants ofP. edulis, only plants ofP. malacophyllaremained uninfected and the virus was not detected by PCR, suggesting immunity to this begomovirus. Plants ofDatura stramonium,Nicotiana benthamiana,N. clevelandii,N. tabacum‘Xanthi’,Solanum lycopersicum(tomato cv. Compack) and several varieties ofManihot esculenta(cassava cvs IAC 118‐96, IAC 6‐01, Paranavaí, IAC 576, IAC 90 and IAC 14) were susceptible to infection with PSLDV through transmission withB. tabaciMEAM1. Adults ofB.tabaciMEAM1 acquired PSLDV after 1 h of feeding on PSLDV‐infected plants ofD.stramonium.They transmitted the virus to healthy plants after feeding on these plants for 1 h, although with low transmission rates. The retention time of PSLDV in the vector was 14 days.

A de novo long-read genome assembly of the sacred datura plant (Datura wrightii) reveals a role of tandem gene duplications in the evolution of herbivore-defense response

The sacred datura plant (Solanales: Solanaceae: Datura wrightii) has been used to study plant–herbivore interactions for decades. The wealth of information that has resulted leads it to have potential as a model system for studying the ecological and evolutionary genomics of these interactions. We present a de novo Datura wrightii genome assembled using PacBio HiFi long-reads. Our assembly is highly complete and contiguous (N50 = 179Mb, BUSCO Complete = 97.6%). We successfully detected a previously documented ancient whole genome duplication using our assembly and have classified the gene duplication history that generated its coding sequence content. We use it as the basis for a genome-guided differential expression analysis to identify the induced responses of this plant to one of its specialized herbivores (Coleoptera: Chrysomelidae: Lema daturaphila). We find over 3000 differentially expressed genes associated with herbivory and that elevated expression levels of over 200 genes last for several days. We also combined our analyses to determine the role that different gene duplication categories have played in the evolution of Datura-herbivore interactions. We find that tandem duplications have expanded multiple functional groups of herbivore responsive genes with defensive functions, including UGT-glycosyltranserases, oxidoreductase enzymes, and peptidase inhibitors. Overall, our results expand our knowledge of herbivore-induced plant transcriptional responses and the evolutionary history of the underlying herbivore-response genes.

Effects of phosphorus fertilizer and nano-magnesium spray on the vegetative and reproductive of Datura stramonium L.

In 2021, a field experiment was conducted at the College of Agriculture, University of Kerbala, Kerbala, Iraq, to study the effect of phosphate fertilization1 and nano-magnesium spraying on Datura plants' vegetative and reproductive traits. The experiment was conducted with three replications according to randomized complete block design (RCBD) with two factors. The first factor included four levels of Phosphorus fertilizer: 0, 25, 50, and 75 kg P h-1. The second factor included spraying of nano-magnesium in four concentrations: 0 (distilled water only) and 60, 120, and 180 mg Mg L-1. The results showed that phosphorous caused a significant increase in the studied traits, as the treatment 50 kg P ha-1 gave the highest mean plant height, number of branches, number of leaves, leaf area, leaf yield, seed yield, alkaloids percentage in leaves and seeds, number of fruits and number of seeds, which amounted to 4953 cm and 27.62 plant-1, 124.49 leaves Plant-1, 1989.12 cm2, 501.36 kg ha-1, 501.49 kg ha-1, 13.16%, 13.18%, 26.02 fruit plant-1 and 228.14 seed plant-1, respectively. The treatment 180 kg Mg h-1 gave the highest average plant height, number of branches, number of leaves, leaf area, yield of leaves, yield of seeds, percentage of alkaloids in leaves and seeds, number of fruits and number of seeds reaching 58.27 cm And 21.90 branches Plant-1, 122.94 leaves Plant-1, 1996.62 cm2, 886.59 kg h-1, 486.17 kg h-1, 14.86%, 15.10%, 26.56 fruits plant-1, 24.63 seeds plant-1. Adding phosphorous and spraying nano-magnesium improves the vegetative and reproductive traits of the Datura plant. Keywords. Fertilization; Nano–Nutrients; Medicinal Plant; Yield; alkaloids.

Fe3 O4 and bimetal-organic framework Zn/Mg composite peroxide-like catalyze luminol chemiluminescence for specific measurement of atropine in Datura plant.

Atropine (AT) is an anticholinergic drug. AT is abundantly in Datura plant seeds. Fe3 O4 @Zn/Mg MOF (Fe3 O4 @MOF) composite was synthesized. The compound had a high peroxidase-like activity in a chemiluminescence (CL) reaction. Addition of AT quenched CL. The linear range and limit of detection were 5-600 μg L-1 and 2 × 10-2 μg L-1 . This method is fast, reversible, and selective, without biodegradability effects, high accuracy, and precision for measuring AT in the Datura plant.

Efficacy of Azadirachta indica and Datura stramonium Extract with Ethanol against Different Life Stages of Tribolium castinium

Tribolium castinium is a devastating stored grain pests of many cereals. Both the larval and adult stages of insect are considered as voracious feeder of cereals. The adult of T. castinium secrete certain type of excretion that give foul smell from grains and make it unfit for consumption. The recent study was conducted in Department of Entomology College of Agriculture Bahauddin Zakariya University, Bahadur sub campus Layyah. Neem (Azadirachta indica)L. and Datura (Datura stramonium)L. ethanol extracts were tested for their repellant and toxicological effects against the larval and adult stages of T. castinium at varying concentrations of 10, 15, and 30ml. The results showed that D. stramonium plant extract recorded highest repellency (86.66%) and the lowest repellency (4.50%) against T. castinium. Minimum mortality was reported at 65% in plant extract of A. indica after an interval of 24 hours at 10ml concentration, and maximum mortality was recorded (90.69%) in plant extract of D. stramonium at 30ml concentration after an interval of 72 hours. The results showed that neem and Datura plant extracts were more effective at repelling and toxicologically acting against T. castinium. The larval stage of T. castinium was also particularly vulnerable to D. stramonium and A. indica ethanol extract.

Effect of Plant Defenses and Plant Nutrients on the Performance of Specialist and Generalist Herbivores of Datura: A Macroevolutionary Study.

Macroevolutionary patterns in the association between plant species and their herbivores result from ecological divergence promoted by, among other factors, plants' defenses and nutritional quality, and herbivore adaptations. Here, we assessed the performance of the herbivores Lema trilineata daturaphila, a trophic specialist on Datura, and Spodoptera frugiperda, a polyphagous pest herbivore, when fed with species of Datura. We used comparative phylogenetics and multivariate methods to examine the effects of Datura species' tropane alkaloids, leaf trichomes, and plant macronutrients on the two herbivores´ performances (amount of food consumed, number of damaged leaves, larval biomass increment, and larval growth efficiency). The results indicate that species of Datura do vary in their general suitability as food host for the two herbivores. Overall, the specialist performs better than the generalist herbivore across Datura species, and performance of both herbivores is associated with suites of plant defenses and nutrient characteristics. Leaf trichomes and major alkaloids of the Datura species are strongly related to herbivores' food consumption and biomass increase. Although hyoscyamine better predicts the key components of the performance of the specialist herbivore, scopolamine better predicts the performance of the generalist; however, only leaf trichomes are implicated in most performance components of the two herbivores. Nutrient quality more widely predicts the performance of the generalist herbivore. The contrasting effects of plant traits and the performances of herbivores could be related to adaptive differences to cope with plant toxins and achieve nutrient balance and evolutionary trade-offs and synergisms between plant traits to deal with a diverse community of herbivores.

Determination of Atropine by HPLC in Plant of Datura by Liquid-Liquid Extraction and Magnet Solid-Phase Extraction.

Atropine is a tropane alkaloid found in abundance in Datura plant. We used two liquid-liquid extraction methods and magnet solid-phase extraction to compare the amount of atropine in these two types of plants (Datura innoxia and Datura stramonium). The surface magnetic nanoparticle Fe3O4 correction with an amine and dextrin, and finally, magnetic solid-phase extraction Fe3O4@SiO2-NH2-dextrin (MNPs-dextrin), was prepared. We determined the effect of significant parameters in the removal step and optimization of atropine measurements with half-fractional factorial design (25-1) and response surface methodology via central composite design. The optimum conditions are for desorption solvent = 0.5mL methanol and desorption time of 5min. We obtained an extraction recovery of 87.63% with a relative standard deviation of 4.73% via six frequented measurements on a 1μgL-1 atropine standard solution based on the optimum condition. Preconcentration factors for MNPs are 81, limit of detection = 0.76μgL-1 and limit of quantitation = 2.5μgL-1.

Impact of sewage water irrigation on Datura innoxia grown in sandy loam soil

BackgroundA potential solution for recycling and reusing the massively produced sewage water (SW) is to irrigate certain plants instead of highly cost recycling treatment. Although the extensive and irrational application of SW may cause environmental pollution thus, continual monitoring of the redox status of the receiver plant and the feedback on its growth under application becomes an emergent instance. The impact of SW, along with well water (WW) irrigation of medicinal plant, Datura innoxia, was monitored by some physio-biochemical indices.ResultsThe SW application amplified the growth, yield, minerals uptake, and quality of D. innoxia plants compared to the WW irrigated plants. The total chlorophyll, carotenoid, non-enzymatic antioxidants, viz. anthocyanin, flavonoids, phenolic compounds, and total alkaloids increased by 85, 38, 81, 50, 19, and 37%, respectively, above WW irrigated plants. The experiment terminated in enhanced leaf content of N, P, and K by 43, 118, and 48%, respectively. Moreover, stimulation of carbon and nitrogen metabolites in terms of proteins, soluble sugars, nitrate reductase (NR) activity, and nitric oxide (NO) content showed significant earliness in flowering time. The SW application improved not only Datura plants’ quality but also soil quality. After four weeks of irrigation, the WW irrigated plants encountered nutrient deficiency-induced stress evidenced by the high level of proline, H2O2, and MDA as well as high enzyme capabilities. Application of SW for irrigation of D. innoxia plant showed the improvement of secondary metabolites regulating enzyme phenylalanine ammonia-lyase (PAL), restored proline content, and cell redox status reflecting high optimal condition for efficient cellular metabolism and performance along the experiment duration.ConclusionsThese evidences approved the benefits of practicing SW to improve the yield and quality of D. innoxia and the feasibility of generalization on multipurpose plants grown in poor soil.

Tropane alkaloids (hyoscyamine, scopolamine and atropine) from genus Datura: extractions, contents, syntheses and effects

Belonging to family Solanaceae , genus Datura is widely distributed all over the world and famous for its narcosis and acesodyne in ancient China. And Datura is also annoying due to the poisoning and hallucinogenic effects. Besides the medicine, it plays a magical role in seed germination and plant growth, and has good effects against plant diseases, insectsand weeding. Its main function components are tropane alkaloids (TAs), including hyoscyamine , scopolamine and atropine (HSA). They share a common tropane fragment (N-methyl-8-azabicyclo[3.2.1] octane). L -hyoscyamine and scopolamine are natural active ingredients in Datura , while atropine is an equal mixture of L -hyoscyamine and D -hyoscyamine, produced by the racemization of L -hyoscyamine during the extraction. As competitive antagonists of acetylcholine muscarinic (M-) choline receptors, HSA potently regulates the central nervous system (CNS). In addition, scopolamine shows effectiveness in the treatment of the respiratory and digestive system diseases, such as acute respiratory distress syndrome (ARDS) and gastrointestinal motility disorders. Hyoscyamine has remarkable effects on treating Parkinson's disease, bradyarrhythmias and genitourinary symptoms. Atropine is widely used in the treatment of myopia, amblyopia and organophosphorus poisoning. Recently, scopolamine-induced disease models play an important role in the exploration of the effects of drugs in amnesia, cognitive impairment and neuronal damage. In this review, we aim to summarize the contents of HSA in different Datura species and their influencing factors, performe an overview of the biosynthesis and chemical synthesis pathways and analyze the key synthetase in the NCBI database, highlight the effect of Datura extract and HSA on resisting plant insects and diseases, controlling weeds and treating different kinds of diseases in human. • The efficient extraction methods of Datura extract are sorted and compared. • The contents of HSA in different Datura species and plant organs vary greatly. • The key HAS synthase genes identified in Solanaceae are less studied in Datura . • HSA play important role in medicine, plant disease control and insect resistance. • HSA have potential in biological control and medicinal industrialized production.

Antiviral Agents and Biological Preparations for Agriculture Based on Artificial Glycan-Glycolipid Complexes

On the way of development of antiviral agents for agriculture, artificial glycan-glycolipid complexes (GGC) were created from glucan, which was the component of mycelium of basidiomicotafungi Ganoderma adspersum (Schulzer) Donk. Other constituents are extracellular glucouronoxylomannan of basidiomicota fungi Tre-mella mesenteric Ritz. Fr., mannan from Candida maltosa cells. Ramnolipid of Pseudomonas sp. PS-17 is used as a compound agent, аnd GGC fractions (liposomes and supernatant) have an inhibit in gactivity against virus of tabacum mosaic (VТM) of datura (Datura stramonium (L.) and tabacum (Nicotiana tabacum L.) plants up-sensitive to this virus. Under by the treatment of soybean (Glycine max (L.) Merr.) Seeds bio formulations, the plant resistance to mosaic virus infections (diseases) and the reflection of leaf light spectra, which characterized of chlorophill sunder field conditions, are increased. Using the electron microscope method of investigation, the structures of microcenoses as well as liposoms were found out in the near-root plant zone, which indicates on the lack of impact to the processes of plant-rhyzospheric microorganism’s interactions. The investigation has shown that pre-sowing bacterization by Brady rhizobium japonicum UCM B-6018 in combination with GGC-3 preparation promotes the crop increase in field experiments.

Assessment of fungitoxic effects of botanicals on the management of growth in Colletotrichum lindemuthianum, the incitant of cowpea anthracnose

Antifungal activities of thirty six botanicals were assessed with an objective to find out the potential of botanicals against the mycelia growth of Colletotrichum lindemuthianum in vitro. Plant specimens were collected locally based on its medicinal, antimicrobial properties and extracted using aqueous means. The botanicals were tried in six different concentrations. Strychnos, Rauwolfia, Pongamia, Azadirachta, Datura, Zingiber, Allium cepa, Allium sativum, Eucalyptus, Aegel and Momordica plant extracts at 25% and 30% concentration arrested the growth of the mycelia to the tune of 100% which can be recommended for the use in field.

Pharmacognostic and Phytochemical Screening of Datura stramonium by TLC and GC-MS: A Forensic Approach

Forensic Botany helps in linking the location, type of crime, revealing modus Operandi, culprit as well as victim by various circumstantial facts. In the study, Datura stramonium was considered as major plant of Bundelkhand region encountered in poisoning cases in criminal Investigations. Datura plant was identified and its fresh leaves were washed to remove dirt, stored at 40C and were subjected to microscopic examination for botanical features, preliminary screening for alkaloids identification and chromatographic techniques such as TLC and GCMS for qualitative analysis of toxins present in the plant. The result showed that all techniques applied are suitable to identify the plant through botanical derived evidences such as leaf traces, resins, or plant extracts during the crime scene investigations. GCMS technique identified the main toxins and resulted that it contains high concentration of hyoscine and very fatal when administered eventually causes death.

Devil’s trumpet poisoning: A case report

Datura is a common plant in India that grows abundantly in the wild across the country. The common names of datura plant are Jimson weed, Stinkweed, Devil’s trumpet, Mad apple and Thorn apple etc. It is considered as a deliriant poison. Very few cases of intentional poisoning with Datura are reported in the literature. But accidental poisonings are though rare but not uncommon.Hereby we present a case of 37 years old male, who got poisoned by datura accidentally, following which he developed giddiness and was brought to our hospital. The diagnosis and treatment of the datura poisoning will be discussed.

Different Methods for Detection and Diagnosis of Tomato Spotted Wilt Tospovirus Infection of some Cucurbit Host Plants and its Main Vector of Thrips Species in Field and Greenhouse in Egypt

The study recorded for the first time in Egypt the presence of tomato spotted wilt virus (TSWV) on cucurbits, which was confirmed using several serological tests, like double antibody sandwich ELISA (DAS-ELISA); Dot-blot ELISA assay and Immuno capture polymerase chain reaction (IC-PCR). By using Dot blot immunoassay test reaction against TSWV antisera, the ability of thrips species Thrips tabaci (Lind), Thrips palmi (Karny), and Frankliniella occidentalis (​Pergande), on the transmission of the (TSWV) were asserted. In the greenhouse, eleven plant species were determined as the host range of virus and field samples showed the similar symptoms. The mechanical transmission of TSWV showed a positive reaction bearing symptoms on the leaves of Cucumber, zucchini, smoke, datura and tomato plants 15-20 days after inoculation with transmission rates of 46.5, 40, 46.5, 46.5 and 86.5%, respectively. DAS-ELISA using antisera of TSWV showed that the greenhouse leaves samples were infected; otherwise, an amplification of IC-PCR products of 500 bp was detected by agarose electrophoresis of total RNA isolated from infected leaves.

Nematicidal activity of the tropane alkaloids hyoscyamine and scopolamine against the root-knot nematode Meloidogyne incognita

Summary Alkaloids and alkaloid-producing plants have the potential to reduce crop damage by plant-parasitic nematodes. In a series of in vitro experiments, the nematicidal activity of the tropane alkaloids, hyoscyamine and scopolamine, and a mixture of both on the root-knot nematode, Meloidogyne incognita, was tested. Solutions of 16 mg of compound per ml of solvent were used in concentrations of 80-1280 μg ml−1 of water. Inactivity of second-stage juveniles of M. incognita increased with increasing concentration and exposure time. Lethal concentrations (LC50) after 120 min of exposure ranged from 182.4 μg ml−1 for scopolamine to 318.4 μg ml−1 for hyoscyamine and 332.8 μg ml−1 for the combination of both alkaloids. Similarly, the same concentrations of scopolamine inhibited hatching of M. incognita to a greater extent than hyoscyamine. In a glasshouse experiment, M. incognita was not able to penetrate the roots, induce galls and reproduce on Datura stramonium, D. innoxia and D. tatula. Results indicate that the tropane alkaloids hyoscyamine and scopolamine contained in Datura plants express a strong nematicidal activity against M. incognita and could possibly be used for an alternative and sustainable nematode management.

Effects of datumetine on hippocampal NMDAR activity.

The usage (abuse) of Datura metel is becoming increasingly worrisome among the Nigerian populace especially among the youth considering its side effects such as hallucination. This work was designed to identify the phytochemicals in datura plant that potentially interact with NMDAR as it affects the electrical and memory activities of the brain. Ligand-protein interaction was assessed using autodock vina to identify phytochemicals that can interact with NMDAR. Datumetine was found to have the best interaction fit with NMDAR at both allosteric and orthosteric binding sites. Furthermore, using electrophysiological, behavioural and western blotting techniques, it was observed that the administration of datumetine positively modulates the NMDAR current by prolonging burst duration and interspike interval, induces seizures in C57BL/6 mice. Acute exposure leads to memory deficit on NOR and Y-maze test while immunoblotting results showed increased expression of GluN1 and CamKIIα while pCamKIIα-T286, CREB and BDNF were downregulated. The results showed that the memory deficit seen in datura intoxication is possibly the effects of datumetine on NMDAR.