Data Processing Statistics Research Articles

Formation of future specialists in the specialty of human health and physical recreation of the ability to use the indicator of a person's blood group

One of the urgent issues of training specialists in the specialty "Human health and physical recreation" is the formation of skills to carry out valeodiagnosis and valeocorrection according to the blood group indicator. The author's rehabilitation program requires individualization using personality parameters. One of the parameters of individualization is the indicator of a person's blood group. The purpose of the presented research was to create a methodical set of laboratory works for the disciplines "Sports biology with the basics of sports genetics", "Diagnostics and health monitoring", "Fundamentals of sports pharmacology and phytology". A chemical model of blood group determination and hemagglutination reaction has been created, which allows you to visualize the mechanism of blood group determination using monoclonal antibodies without the use of human blood. The presented model also demonstrates multiple allelism, the law of purity of gametes, the process of inheritance of blood groups. The paper also presents the results of an experiment on determining blood groups using plant lectins and an algorithm for determining blood groups using lectin-containing extracts of the four-winged licorice, mouse pea, Japanese sophora, and meadowsweet. Laboratory works "Blood groups and personal portrait of a person" and "Individualization of lifestyle according to the map of blood groups" were developed based on literary data. The work also presents the results of a pedagogical experiment. Data processing statistics show that the laboratory method increases the level of students' knowledge by 24.7%.

Investigation of the milling characteristics of different focused-ion-beam sources assessed by three-dimensional electron diffraction from crystal lamellae.

Three-dimensional electron diffraction (3DED) from nanocrystals of biological macromolecules requires the use of very small crystals. These are typically less than 300 nm-thick in the direction of the electron beam due to the strong interaction between electrons and matter. In recent years, focused-ion-beam (FIB) milling has been used in the preparation of thin samples for 3DED. These instruments typically use a gallium liquid metal ion source. Inductively coupled plasma (ICP) sources in principle offer faster milling rates. Little work has been done to quantify the damage these sources cause to delicate biological samples at cryogenic temperatures. Here, an analysis of the effect that milling with plasma FIB (pFIB) instrumentation has on lysozyme crystals is presented. This work evaluates both argon and xenon plasmas and compares them with crystals milled with a gallium source. A milling protocol was employed that utilizes an overtilt to produce wedge-shaped lamellae with a shallow thickness gradient which yielded very thin crystalline samples. 3DED data were then acquired and standard data-processing statistics were employed to assess the quality of the diffraction data. An upper bound to the depth of the pFIB-milling damage layer of between 42.5 and 50 nm is reported, corresponding to half the thickness of the thinnest lamellae that resulted in usable diffraction data. A lower bound of between 32.5 and 40 nm is also reported, based on a literature survey of the minimum amount of diffracting material required for 3DED.

IMPLEMENTASI PERANGKAT PREDICTIVE DIALING SYSTEM DAMPAKNYA TERHADAP KINERJA TIM FRONTEND PADA UNIT COLLECTION, BNI CARD CENTER

This research is to know how much impact the implementation of the PDS device on team Frontend performance, Collection Unit, BNI Card Center, Card Business Center Bandung. On the other hand, a credit card as a form of unsecured loans bad credit has the potential to give birth. BNI Bank as one of the credit card issuer bank has a Collection unit on the Card Business division of one of its tasks to restore the company's assets that minimize the number of Net-Performing Loans (NPL). To support these tasks, the BNI Card Center management implements Predictive Dialing System (PDS) device.
 In conducting this research, the author uses descriptive research methods Quantitative Analysis. To measure the quality factor of Predictive Dialing System was used Functionality, Usability, Reliability, Performance, and Supportability factor. And to measure Frontend team performance used Kept Account and Kept Payment factor. The Data processing statistics used SPSS 15 for Windows. 
 The Implementation of PDS devices has given a providing impact or effect of 62.1 percent on Frontend team performance, Collection unit, BNI Card Center, Card Business Center Bandung. Based on test results above proved that the implementation of the PDS has a significant impact in increasing the Frontend team performance. The better implementation of the PDS is expected to improve Frontend team performance on Collection unit, BNI Card Center, Card Business Center Bandung, nor vice versa.

Kurumsal Hafıza ve Örgütsel Öğrenmenin Kurum Kültürü Aracılığıyla Firma Performansı Üzerindeki Etkisi

The main purpose of the study is to determine to what extent and in what direction corporate memory and organizational learning affect firm performance through corporate culture. Within the framework of the research model, the relationships between the sub-dimensions of corporate memory and organizational learning, which are the independent variables, and the sub-dimensions of firm performance, which is the dependent variable, are examined. Within the scope of the study, the mediating role of corporate culture in relations between variables is also questioned. The study is carried out with comprehensive research on Information and Technology companies operating in Istanbul. The survey method is chosen as the data collection method and 7-point Likert-type expressions are used in the scales. 635 questionnaires are collected from Information and Technology companies in the Marmara Region and the hypotheses in the research model are analyzed using the SPSS data processing statistics program. As a result of the analysis, it is determined that corporate culture, organizational learning and corporate memory have ssignificant effects on firm performance. However, it is determined that the mediation role of corporate culture has a partial effect on the effect of corporate memory and organizational learning on firm performance.

Sosialisasi Media Pembelajaran dan Program Statistika di SD Negeri Setu 01 Cipayung

The teaching and learning process is essentially a communication process, which is the process of delivering messages from the message source through certain channels / media to the recipient of the message. In teaching and learning activities, the media is one of the factors that determine the success of the learning process. Teaching media such as snakes and ladders, domino cards and monopoly games can enhance the learning process of students in teaching, which in turn is expected to enhance the learning outcomes achieved. In PTK (Classroom Action Research) conducted by teachers, teachers can develop in terms of the use and use of learning media to improve the quality of learning in the classroom. Training in the use of instructional media and training in research data processing statistics programs to develop the quality of CAR carried out by teachers in schools is useful for improving the skills of elementary school teachers in using software SPSS in processing Classroom Action Research (PTK) data.

The predictive power of data-processing statistics.

This study describes a method to estimate the likelihood of success in determining a macromolecular structure by X-ray crystallography and experimental single-wavelength anomalous dispersion (SAD) or multiple-wavelength anomalous dispersion (MAD) phasing based on initial data-processing statistics and sample crystal properties. Such a predictive tool can rapidly assess the usefulness of data and guide the collection of an optimal data set. The increase in data rates from modern macromolecular crystallography beamlines, together with a demand from users for real-time feedback, has led to pressure on computational resources and a need for smarter data handling. Statistical and machine-learning methods have been applied to construct a classifier that displays 95% accuracy for training and testing data sets compiled from 440 solved structures. Applying this classifier to new data achieved 79% accuracy. These scores already provide clear guidance as to the effective use of computing resources and offer a starting point for a personalized data-collection assistant.

How good are my data and what is the resolution?

Following integration of the observed diffraction spots, the process of `data reduction' initially aims to determine the point-group symmetry of the data and the likely space group. This can be performed with the program POINTLESS. The scaling program then puts all the measurements on a common scale, averages measurements of symmetry-related reflections (using the symmetry determined previously) and produces many statistics that provide the first important measures of data quality. A new scaling program, AIMLESS, implements scaling models similar to those in SCALA but adds some additional analyses. From the analyses, a number of decisions can be made about the quality of the data and whether some measurements should be discarded. The effective `resolution' of a data set is a difficult and possibly contentious question (particularly with referees of papers) and this is discussed in the light of tests comparing the data-processing statistics with trials of refinement against observed and simulated data, and automated model-building and comparison of maps calculated with different resolution limits. These trials show that adding weak high-resolution data beyond the commonly used limits may make some improvement and does no harm.

Crystal Structure of Plant Ferritin Reveals a Novel Metal Binding Site That Functions as a Transit Site for Metal Transfer in Ferritin

Ferritins are important iron storage and detoxification proteins that are widely distributed in living kingdoms. Because plant ferritin possesses both a ferroxidase site and a ferrihydrite nucleation site, it is a suitable model for studying the mechanism of iron storage in ferritin. This article presents for the first time the crystal structure of a plant ferritin from soybean at 1.8-A resolution. The soybean ferritin 4 (SFER4) had a high structural similarity to vertebrate ferritin, except for the N-terminal extension region, the C-terminal short helix E, and the end of the BC-loop. Similar to the crystal structures of other ferritins, metal binding sites were observed in the iron entry channel, ferroxidase center, and nucleation site of SFER4. In addition to these conventional sites, a novel metal binding site was discovered intermediate between the iron entry channel and the ferroxidase site. This site was coordinated by the acidic side chain of Glu(173) and carbonyl oxygen of Thr(168), which correspond, respectively, to Glu(140) and Thr(135) of human H chain ferritin according to their sequences. A comparison of the ferroxidase activities of the native and the E173A mutant of SFER4 clearly showed a delay in the iron oxidation rate of the mutant. This indicated that the glutamate residue functions as a transit site of iron from the 3-fold entry channel to the ferroxidase site, which may be universal among ferritins.

Crystal Structure of the LG1-3 Region of the Laminin α2 Chain

Laminins are large heterotrimeric glycoproteins with many essential functions in basement membrane assembly and function. Cell adhesion to laminins is mediated by a tandem of five laminin G-like (LG) domains at the C terminus of the α chain. Integrin binding requires an intact LG1-3 region, as well as contributions from the coiled coil formed by the α, β, and γ chains. We have determined the crystal structure at 2.8-Å resolution of the LG1-3 region of the laminin α2 chain (α2LG1-3). The three LG domains adopt typical β-sandwich folds, with canonical calcium binding sites in LG1 and LG2. LG2 and LG3 interact through a substantial interface, but LG1 is completely dissociated from the LG2-3 pair. We suggest that the missing γ chain tail may be required to stabilize the interaction between LG1 and LG2-3 in the biologically active conformation. A global analysis of N-linked glycosylation sites shows that the β-sandwich faces of LG1 are free of carbohydrate modifications in all five laminin α chains, suggesting that these surfaces may harbor the integrin binding site. The α2LG1-3 structure provides the first atomic view of the integrin binding region of laminins.

High Affinity Interaction between a Bivalve C-type Lectin and a Biantennary Complex-type N-Glycan Revealed by Crystallography and Microcalorimetry

Codakine is an abundant 14-kDa mannose-binding C-type lectin isolated from the gills of the sea bivalve Codakia orbicularis. Binding studies using inhibition of hemagglutination indicated specificity for mannose and fucose monosaccharides. Further experiments using a glycan array demonstrated, however, a very fine specificity for N-linked biantennary complex-type glycans. An unusually high affinity was measured by titration microcalorimetry performed with a biantennary Asn-linked nonasaccharide. The crystal structure of the native lectin at 1.3A resolution revealed a new type of disulfide-bridged homodimer. Each monomer displays three intramolecular disulfide bridges and contains only one calcium ion located in the canonical binding site that is occupied by a glycerol molecule. The structure of the complex between Asn-linked nonasaccharide and codakine has been solved at 1.7A resolution. All residues could be located in the electron density map, except for the capping beta1-4-linked galactosides. The alpha1-6-linked mannose binds to calcium by coordinating the O3 and O4 hydroxyl groups. The GlcNAc moiety of the alpha1,6 arm engages in several hydrogen bonds with the protein, whereas the GlcNAc on the other antenna is stacked against Trp(108), forming an extended binding site. This is the first structural report for a bivalve lectin.

Crystal Structure of Mouse Carnitine Octanoyltransferase and Molecular Determinants of Substrate Selectivity*[boxs

Carnitine acyltransferases have crucial functions in fatty acid metabolism. Members of this enzyme family show distinctive substrate preferences for short-, medium- or long-chain fatty acids. The molecular mechanism for this substrate selectivity is not clear as so far only the structure of carnitine acetyltransferase has been determined. To further our understanding of these important enzymes, we report here the crystal structures at up to 2.0-A resolution of mouse carnitine octanoyltransferase alone and in complex with the substrate octanoylcarnitine. The structures reveal significant differences in the acyl group binding pocket between carnitine octanoyltransferase and carnitine acetyltransferase. Amino acid substitutions and structural changes produce a larger hydrophobic pocket that binds the octanoyl group in an extended conformation. Mutation of a single residue (Gly-553) in this pocket can change the substrate preference between short- and medium-chain acyl groups. The side chains of Cys-323 and Met-335 at the bottom of this pocket assume dual conformations in the substrate complex, and mutagenesis studies suggest that the Met-335 residue is important for catalysis.

Structure of HI1333 (YhbY), a putative RNA-binding protein from Haemophilus influenzae.

Structure of HI1333 (YhbY), a putative RNA-binding protein from Haemophilus influenzae.

Functional relevance of the disulfide-linked complex of the N-terminal PDZ domain of InaD with NorpA.

In Drosophila, phototransduction is mediated by G(q)-activation of phospholipase C and is a well studied model system for understanding the kinetics of signal initiation, propagation and termination controlled by G proteins. The proper intracellular targeting and spatial arrangement of most proteins involved in fly phototransduction require the multi-domain scaffolding protein InaD, composed almost entirely of five PDZ domains, which independently bind various proteins including NorpA, the relevant phospho lipase C-beta isozyme. We have determined the crystal structure of the N-terminal PDZ domain of InaD bound to a peptide corresponding to the C-terminus of NorpA to 1.8 A resolution. The structure highlights an intermolecular disulfide bond necessary for high affinity interaction as determined by both in vitro and in vivo studies. Since other proteins also possess similar, cysteine-containing consensus sequences for binding PDZ domains, this disulfide-mediated 'dock-and-lock' interaction of PDZ domains with their ligands may be a relatively ubiquitous mode of coordinating signaling pathways.

Macromolecular crystallography with synchrotron radiation. II. Results

Crystallographic data for three different protein crystals (glycogen phosphorylase b to 2 Å resolution, β-lactamase I to 2.5 Å resolution and troponin C to 6 Å resolution) have been recorded using the intense synchrotron radiation beam emitted by the DCI storage ring at LURE and the DORIS storage ring at DESY/EMBL. Reduction in exposure times of approximately 50-fold and an increase in crystal lifetime of at least fivefold are observed when data recorded at LURE are compared with those recorded with a conventional rotating-anode source. These factors have made possible data collection which otherwise would have been impossible. For large crystals of phosphorylase b a greater reduction in exposure time ( × 125) is made possible by the focusing geometry of the synchrotron-monochromator system which allowed irradiation of a larger volume of the crystal (collimator size increased from 0.3 to 1.0 mm) without significant increase in spot overlap on the film. The data processing statistics for phosphorylase b and β-lactamase compare favourably with those from data recorded on a conventional source (improvements in merging R values of between 1 and 4%). For phosphorylase b, but not for β-lactamase or troponin C, significant thermal diffuse scatter is observed on photographs recorded with synchrotron radiation. The possible origin of this phenomenon and its effect on data processing are discussed.