We evaluated the toxicity and sublethal effects of essential oil (Mentha pulegium L.) and its nanoformulation against greenhouse whitefly, Trialeurodes vaporariorum, which is one of the most destructive pests of a wide range of crops. The essential oil was extracted from the plant by steam distillation using a Clevenger apparatus, and 14 chemical components of M. pulegium were identified using gas chromatography-mass spectrometry. The results illustrated that monoterpenoids were main characterized components including pulegone (%66), menthofren (%10.54), 1, 8 Cineole (%8.36), betapenin (%3.49) and limonene (%2.01). The nanoformulation was characterized using dynamic light scattering (DLS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM), revealing that the particles were spherical in shape with an average size of 156.40 nm. The leaf dipping was used for the bioassays. The obtained LC50 and LC25 values of treatments indicated that the nanoformulation of essential oil (LC50: 2418.96 and LC25: 1724. 25 ppm) was more toxic than the pure of M. pulegium oil (LC50: 3223.083 and LC25: 779.439 ppm ppm) against greenhouse whitefly adults after 24 h. The life table data were analyzed based on the age-stage, two-sex life table theory using computer program of TWOSEX–MSChart. Also, the sublethal concentration (LC25) of its nanoformulation led to delaying in preadult stage and decreased the adult longevity, and fecundity compared to treatments. Moreover, the sublethal concentration of either M. pulegium oil or its nanoformulation affected the population growth parameters of T.vaporariorum compared to the control. However, the net reproductive rate (R0), intrinsic rate of increase (r), finite rate of increase (λ), of adults who exposed to the nanoformulation was lower than the pure form of M. pulegium. The overall results demonstrated that the nanoformulation of M. pulegium has the most lethal and sublethal effects on greenhouse whitefly compared with the pure form of essential oil which can be consider in integrated pest management program (IPM) of this pest.