Dark-field Setup Research Articles

Dark-field setup for the measurement of light-by-light scattering with high-intensity lasers

Dark-field setup for the measurement of light-by-light scattering with high-intensity lasers

Scattering spectroscopy on single plasmonic nanoparticles using a confocal darkfield setup

We demonstrate how to measure scattering spectra of single plasmonic nanoparticles using a confocal darkfield setup. We give an overview of considerations and problems we encountered when employing a darkfield technique based on filtering illumination under low angles instead of the conventional high-angle filtering by darkfield objectives.

X-Ray Dark-field radiography: Does this carry potential for diagnosing gout in exotic pets?

The aim of this study is to evaluate whether X-ray dark-field (DF) radiography is useful for the diagnosis of gout in birds and reptiles and whether this preclinical model could be helpful to establish this non-invasive imaging method in human medicine. A total of 18 limbs originating from 11 birds (7 different species) and 7 reptiles (4 different species) with and without suspected joint gout were measured using a grating-based X-ray dark-field setup and conventional X-ray examination, respectively. Each image acquisition generated a dark-field and a conventional absorption x-ray image. The results of the individual scans were compared with the results of a pathological examination and arthrocentesis. In 5 of the birds and 4 of the reptiles examined, gout was detected by pathologic examination. In each group, uric acid crystals were found in the joints of 3 animals by means of arthrocentesis. The uric acid crystals were detectable in 2 bird and 2 reptile limbs in the dark-field image. The study demonstrated that the urate crystals evoke a clearly visible dark field signal, whereas this was not the case in the conventional radiographs. The results obtained show that uric acid crystal detection using less invasive imaging methods in an animal model with birds and reptiles may expand gout diagnostics not only in veterinary medicine but also in human medicine and possibly replace arthrocentesis if a DF signal is detectable. Preclinical scanners which use X-ray dark-field and phase-contrast radiography already exist for hands and mammography.

Plexcitonic Quantum Light Emission from Nanoparticle-on-Mirror Cavities.

We investigate the quantum-optical properties of the light emitted by a nanoparticle-on-mirror cavity filled with a single quantum emitter. Inspired by recent experiments, we model a dark-field setup and explore the photon statistics of the scattered light under grazing laser illumination. Exploiting analytical solutions to Maxwell’s equations, we quantize the nanophotonic cavity fields and describe the formation of plasmon–exciton polaritons (or plexcitons) in the system. This way, we reveal that the rich plasmonic spectrum of the nanocavity offers unexplored mechanisms for nonclassical light generation that are more efficient than the resonant interaction between the emitter natural transition and the brightest optical mode. Specifically, we find three different sample configurations in which strongly antibunched light is produced. Finally, we illustrate the power of our approach by showing that the introduction of a second emitter in the platform can enhance photon correlations further.

Divided-aperture confocal Brillouin microscopy for simultaneous high-precision topographic and mechanical mapping.

Confocal Brillouin microscopy (CBM) is a novel and powerful technique for providing non-contact and direct readout of the micro-mechanical properties of a material, and thus used in a broad range of applications, including biological tissue detection, cell imaging, and material characterization in manufacturing. However, conventional CBMs have not enabled high precision mechanical mapping owing to the limited depth of focus and are subject to system drift during long-term measurements. In this paper, a divided-aperture confocal Brillouin microscopy (DCBM) is proposed to improve the axial focusing capability, stability, and extinction ratio of CBM. We exploit high-sensitivity divided-aperture confocal technology to achieve an unprecedented 100-fold enhancement in the axial focusing sensitivity of the existing CBMs, reaching 5 nm, and to enhance system stability. In addition, the dark-field setup improves the extinction ratio by 20 dB. To the best of our knowledge, our method achieves the first in situ topographic imaging and mechanical mapping of the sample and provides a new approach for Brillouin scattering applications in material characterization.

X-ray Dark-Field Radiography: Potential for Visualization of Monosodium Urate Deposition.

The aim of this study was to evaluate the potential of x-ray dark-field radiography for the noninvasive detection of monosodium urate (MSU) crystals as a novel diagnostic tool for gout. Contrast-to-noise ratios of MSU crystals in conventional radiography and dark-field radiography have been compared in a proof of principle measurement. Monosodium urate crystals have been injected into mouse legs in an ex vivo experimental gout setup. Three radiologists independently evaluated the images for the occurrence of crystal deposits in a blinded study for attenuation images only, dark-field images only, and with both images available for a comprehensive diagnosis. All imaging experiments have been performed at an experimental x-ray dark-field setup with a 3-grating interferometer, a rotating anode tube (50 kVp), and a photon-counting detector (effective pixel size, 166 μm). X-ray dark-field radiography provided a strong signal increase for MSU crystals in a physiological buffer solution compared with conventional attenuation radiography with a contrast-to-noise ratio increase from 0.8 to 19.3. Based on conventional attenuation images only, the reader study revealed insufficient diagnostic performance (sensitivity, 11%; specificity, 92%) with poor interrater agreement (Cohen's coefficient κ = 0.031). Based on dark-field images, the sensitivity increased to 100%, specificity remained at 92%, and the interrater agreement increased to κ = 0.904. Combined diagnosis based on both image modalities maximized both sensitivity and specificity to 100% with absolute interrater agreement (κ = 1.000). X-ray dark-field radiography enables the detection of MSU crystals in a mouse-based gout model. The simultaneous avaliability of a conventional attenuation image together with the dark-field image provides excellent detection rates of gout deposits with high specificity.

About acquiring and processing light transport matrices for transparent object inspection

Abstract Transparent materials are employed for creating different kinds of products and have to meet high quality requirements. First of all, transparent materials have to be free from scattering defects, e.g., enclosed air bubbles. Visual inspection systems based on dark field setups are principally capable of imaging these kinds of defects, however, it usually requires much effort to adapt them to the test object on hand. This article shows how light transport matrices can be calculated for an optical system consisting of a programmable area light source and a telecentric camera. Two features are proposed that can be extracted out of these matrices and that allow to image scattering defects present in a transparent object without the need of adapting the system to the actual test object. The results of synthetic experiments obtained using a physically based and adequately extended rendering framework approved the proposed approach and showed that it even outperforms classical inspection systems in some situations.

Hyperspectral backscatter imaging: a label-free approach to cytogenetics.

Current techniques for chromosome analysis need to be improved for rapid, economical identification of complex chromosomal defects by sensitive and selective visualisation. In this paper, we present a straightforward method for characterising unstained human metaphase chromosomes. Backscatter imaging in a dark-field setup combined with visible and short near-infrared spectroscopy is used to monitor morphological differences in the distribution of the chromosomal fine structure in human metaphase chromosomes. The reasons for the scattering centres in the fine structure are explained. Changes in the scattering centres during preparation of the metaphases are discussed. FDTD simulations are presented to substantiate the experimental findings. We show that local scattering features consisting of underlying spectral modulations of higher frequencies associated with a high variety of densely packed chromatin can be represented by their scatter profiles even on a sub-microscopic level. The result is independent of the chromosome preparation and structure size. This analytical method constitutes a rapid, cost-effective and label-free cytogenetic technique which can be used in a standard light microscope. Graphical abstract Hyperspectral backscatter imaging for label-free characterization.

Optical Detection of Dust and Scratches on Photographic Film

Today’s information society needs efficient and economic solutions for the digital restoration of the photographic heritage. Different methods have been adopted up to now for the automatic detection of dust and scratches; each method has pros and cons, and a limited field of effectiveness. The use of infrared radiation and the spatiotemporal image analysis are among the most effective methods, although they have their limits. The infrared radiation only works for dye-based material, while the spatiotemporal image analysis is not applicable for still images and is limited due to motion in the scene. The present work defines in detail a set of methods for optical dust and scratches detection applicable on any type of transparent photographic material (silver-based as well as dye-based material, still images as well as moving images). The term “optical” refers to the fact that the considered methods seek physical evidence of the presence of foreign bodies or irregularities on the film; this allows avoiding the typical digital artifacts produced by “nonoptical” methods, for which certain elements of the scenes are erroneously obliterated because they resemble dust grains or scratches. “PDD” (Polarized Dark-field Detection) detects the flaws using an image acquired in a polarized dark-field setup; “DCD” (Dual Collimation Detection) takes advantage of the Callier effect to locate the flaws; “ n -MDD” (Multiple Direction Detection) entails the acquisition of n images in dark-field setups with different directions of illumination, and the extraction of the differences between the images through multivariate analysis. A numerical evaluation of the performances of the MDD method with an eightfold acquisition (8-MDD) is carried out by comparing its flaw detection with the flaw detection provided by commercial software based on spatiotemporal image analysis.

Dark-Field Illumination on Zero-Mode Waveguide/Microfluidic Hybrid Chip Reveals T4 Replisomal Protein Interactions

The ability of zero-mode waveguides (ZMWs) to guide light energy into subwavelength-diameter cylindrical nanoapertures has been exploited for single-molecule fluorescence studies of biomolecules at micromolar concentrations, the typical dissociation constants for biomolecular interactions. Although epi-fluorescence microscopy is now adopted for ZMW-based imaging as an alternative to the commercialized ZMW imaging platform, its suitability and performance awaits rigorous examination. Here, we present conical lens-based dark-field fluorescence microscopy in combination with a ZMW/microfluidic chip for single-molecule fluorescence imaging. We demonstrate that compared to epi-illumination, the dark-field configuration displayed diminished background and noise and enhanced signal-to-noise ratios. This signal-to-noise ratio for imaging using the dark-field setup remains essentially unperturbed by the presence of background fluorescent molecules at micromolar concentration. Our design allowed single-molecule FRET studies that revealed weak DNA–protein and protein–protein interactions found with T4 replisomal proteins.

Sensitive high-resolution white-light Schlieren technique with a large dynamic range for the investigation of ablation dynamics

We developed a modified Hoffman contrast technique with a 12 ns pulsed incoherent extended white-light source that enables an easily interpretable visualization of ablation plumes with high resolution, a large dynamic range, and color information. By comparison, a conventional dark-field setup with a slitlike laser light source provides large sensitivity but a small dynamic range, and it is difficult to interpret the filtered images.