Hesperis Matronalis Research Articles

Assessment of weed species composition of Cabbage family plants (Brassicaceae) of ruderal and segetal habitats of the Ussuriysk urban district of the Primorsky Territory

The data on the species composition of weeds from the Cabbage family (Brassicaceae) that grow stably in the territory of the Ussuriysk urban district of the Primorsky Territory and dominate in agrocenoses are given in the article. Objectives of the study are: to summarize data on the most widespread (background) plant species of natural and adventive flora of the Cabbage family of ruderal and segetal habitats of the Ussuriysk urban district of the Primorsky Territory; to note the species listed in the "Black Book of Flora of the Far East" and growing within the limits of the urban district; to cite the species composition of herbaceous plants of the Cabbage family found in the Ussuriysky Nature Reserve. Field studies were conducted using the traditional route-reconnaissance method in 2010–2023. Phenological observations, geobotanical descriptions of identified habitats, collection, identification of herbarium material and photographing of objects from growing sites were carried out. Of the 23 species of Brassicaceae cited in the paper, ten are invasive in the ecosystems of the Far Eastern Federal District: Armoracia rusticana Gaertn., Mey. et Scherb., Berteroa incana (L.) DC., Brassica juncea (L.) Czern., Bunias orientalis L., Capsella bursa-pastoris (L.) Medik., Hesperis matronalis L., Lepidium densiflorum Schrad., Thlaspi arvense L., Turritis glabra L., Velarum officinale (L.) Reichenb. Most of them, with the exception of B. juncea, C. bursa-pastoris and Th. arvense, are classified as plants that do not cause significant damage at this stage of development. Ruderal habitats are also sites of biodiversity conservation. In the V.L. Komarov Ussuriysky Nature Reserve, 16 species of Cabbage, of which Barbarea orthoceras, B. incana, and Th. arvense are segetal, were identified on the territory of the Ussuriysk Urban District.

Plant survival and growth in bioretention cells under a cold climate

Urbanization coupled with climate change results in significant stormwater management challenges. In this context, the use of green infrastructures such as bioswales and bioretention systems to intercept, slow down and filter runoff has been increasing in North America in recent years. However, little data is available on the selection of optimal plant species adapted to cold climates, their optimal positioning in the swale and intra and inter-seasonal variation in plant growth. A field experiment was conducted during three growing seasons from July 2020 to August 2022 in a proximal suburb of Québec City, Canada, to monitor the survival rate. Growth (height and plant area), and floridity (number of flower buds and flower stems) of 22 plant species in 48 cold climate bioretention cells located along streets. The study aimed to understand the influence of planting position (margin: upper position, usually dryer; slope; middle position, fluctuating water conditions; bottom: lower position, usually moister) on species development, in order to identify the species most and least adapted to the different moisture conditions. Results showed that survival was generally high (> 86%) and was not affected by position within the bioretention cells. However, for most species, plant area increased, and height decreased at the margin compared to the slope position, while floridity only increased at the margin for four species (Heliopsis helianthoides (L.) Sweet, Coreopsis verticillata L., Hemerocallis ‘Stella Supreme” L., Verbena hastata L.). Seven species (Campanula carpatica Jacq “Alba”, Hesperis matronalis L., Osmundastrum cinnamomeum (L.) C. Presl, Rudbeckia hirta L., Verbena hastata L., Cerastium tomentosum L., Athyrium filix-femina (L.) Roth ex Mertens) exhibited lower survival and growth rates over time than the others. Overall, 15 out of 22 species performed well in the bioretention cells with minimum maintenance over the course of the experiment. Results also showed that plant area at the end of the growing season was much more influenced by position in the bioretention cells than were height or floridity, while survival was not influenced by this factor. A better knowledge of plant interactions in varying spatial distribution over time can contribute to improving and potentially maximizing bioretention performance.

TWO-YEAR FLOWER CULTURES IN THE COLLECTION OF THE BOTANICAL GARDEN OF BILA TSERKVA NATIONAL AGRARIAN UNIVERSITY

The article presents the results of the analysis of the collection of two-year-old flowering ornamental plants in the Botanical garden of Bila Tserkva national agrarian university. It was established that the biennial collection includes 17 species belonging to 15 genera and 10 families. The largest number of species is represented by the family Brassicaceae Burnett. – Brassica oleracea L. var. Acephala DC., Erysimum perovskianum Fisch.hort., Hesperis matronalis L., Lunaria biennis L. The collection presents species that have not only decorative, but also medicinal, fodder, and tanning properties. Diditalis grandiflora contains poisonous glycosides. Alcea rosea has ethnographic significance and belongs to amulet plants.
 The morphometric indicators of biennials by height and their flowering periods were analyzed. It was established that 41,2% of the collection of biennials of the Botanical Garden of the Bila Tserkva NAU belongs to low-growing species, medium-sized and tall plants are represented by the same number of species, which is 29,4% each. The largest number of species in the collection is represented by the group of summer-flowering biennials, 47,1%, late-spring-flowering ones make up 29,4%, and early-flowering ones – 23,5% of species. 
 Most biennials are cultivated in open ground to decorate flower beds, only a few to create seasonal bouquets and phytocompositions. Thanks to the plasticity of biennials, which is manifested in non-standard sowing dates, relative frost resistance and early spring and early summer flowering periods, biennials reduce the costs of growing seedlings and care for them after planting in a permanent place, provide an annual change in the design of flower beds and fill the flowerless periods of early spring and early summer. Biennials are suitable for creating borders, flower beds, rabatoks, lawns, carpets, ribbons, for decorating flowerbed groups and balconies, container culture. Tall species can be used as a background for the entire composition. Varietal diversity of Bellis perennis L., Brassica oleracea L. var. Acephala DC, Dianthus caryophyllus L. var. Grenadine hort, Dianthus barbatus L., Viola wittrockiana Gams. allows you to create monoculture flower beds with a wide color palette.

Contrasting responses to climate variability generate seasonal priority effects between native and invasive forest herbs

Abstract Invasive plants are often characterized by rapid germination and precocious phenology. Theory suggests that early germination may provide invaders with a competitive advantage over slower germinating natives, but the relative contribution of rapid germination versus other intrinsic competitive traits (e.g. rapid growth, high fecundity, broad environmental tolerance) to the success of invaders is poorly understood. Depending on the relationship between germination and competition, shifts in germination phenology due to climate change may increase the dominance of invaders or buffer communities against their impacts. We investigated the link between temperature variation, germination phenology and competitive interactions with a sequence of controlled environment experiments. First, we evaluated the relationships between temperature variation and germination phenology for two herbaceous species found in North American woodlands, the non‐native Hesperis matronalis and native Cryptotaenia canadensis. We then leveraged temperature‐response differences to manipulate the relative germination phenology of these taxa and quantified the effects of their phenological differences on competition. Seeds of the invasive H. matronalis germinated rapidly, reaching 50% germination in under 10 days in all treatment combinations. C. canadensis did not reach 50% germination with less than 7 weeks of cold stratification. However, with more than 10 weeks of cold stratification and low (20/10°C) incubation temperatures, the germination phenology of C. canadensis was well‐matched to that of H. matronalis. When grown together, we found that precocious germination phenology doubled the competitive impact of H. matronalis relative to its other intrinsic competitive traits. Phenological advantage of just 2–3 days relative to C. canadensis was enough to secure competitive dominance at the seedling stage. Synthesis. This study revealed that the mechanistic link between the germination phenology and competitive success of an invasive plant can be strongly mediated by climate sensitivity differences between introduced and native species. Climate change will likely exacerbate these differences, especially in regions where warming reduces cold stratification. Our findings suggest that phenological diversity in native plant communities is an important property of invasion resistance. The relationship between environmental variation, germination dynamics and competition provides a path forward for forecasting climate change impacts on seasonal community assembly and highlights the need to incorporate phenological diversity in restorations.

Citizen science and land use data provide insight into the invasive riparian plant composition of the Hudson River Valley watershed

AbstractInvasive plants in the riparian zone can negatively affect the characteristics and quality of a watershed. To support the development of a watershed management plan and foster public appreciation of the value of the riparian zone, Mohonk Preserve established a volunteer monitoring program surveying sites for invasive species. Between 2017 and 2019, citizen scientists repeatedly surveyed 20 sites in the Hudson River Valley in New York for 10 invasive plant species: purple loosestrife (Lythrum salicaria L.), common reed [Phragmites australis (Cav.) Trin. ex Steud], multiflora rose (Rosa multiflora Thunb.), garlic mustard [Alliaria petiolata (M. Bieb.) Cavara & Grande], dame’s rocket (Hesperis matronalis L.), Japanese knotweed (Polygonum cuspidatum Siebold & Zucc.), wineberry (Rubus phoenicolasius Maxim.), barberry (Berberis spp.), Japanese stiltgrass [Microstegium vimineum (Trin.) A. Camus], and Asiatic bittersweet (oriental bittersweet, Celastrus orbiculatus Thunb.). We found that the number of target species detected was higher on sites closer to paved roads and with increasing drainage area size, while lower with higher percentages of forested land in the basin. Our analysis results highlight variation in the presence of target invasive species across the Hudson River Valley region, highlighting sites and areas to monitor for future introductions and take action to prevent species’ invasions. Our results highlight differences in the most relevant abiotic factors for hydrophytes and non-hydrophyte species, underscoring the importance of considering species’ life-history traits before the development of management plans for invasive plant species in the riparian zone. Our case study of community-collected data in the Hudson River Valley region using a relatively simple monitoring protocol can provide a road map for other regions fostering volunteer engagement with invasive plants.

Wild and weedy Hesperis matronalis hosts turnip mosaic virus across heterogeneous landscapes in upstate New York

Turnip mosaic virus (TuMV) is a widespread and economically important pathogen in agricultural crops and has the widest known host range in the virus family Potyviridae. While management of the virus and its aphid vectors in agricultural fields decreases virus incidence, many alternative wild hosts for TuMV may serve as source populations for crop infection through spillover. Over thirty years ago, research demonstrated that the introduced brassica, Dame's Rocket (Hesperis matronalis) hosts several viruses, including TuMV. Here, we use both enzyme-linked immunosorbent assays (ELISA) and next generation sequencing to document the frequent infection by TuMV of Dame's Rocket, which is common and widespread in disturbed areas around crop fields in upstate New York. Deep sequencing of multiple tissue types of symptomatic hosts indicate that the infection is systemic and causes diagnostic, visible symptoms. In a common garden experiment using host populations from across upstate New York, we found evidence for genetic tolerance to TuMV infection in H. matronalis. Field surveys show that TuMV prevalence varies across populations, but is generally higher in agricultural areas. Examining disease dynamics in this and other common alternative hosts will enhance our understanding of TuMV epidemiology and, more broadly, virus distribution in wild plants.

Thirty-two new and noteworthy floristic records from north-eastern Greece.

BackgroundThe vascular flora of Greece comprises no less than 6,705 vascular plant taxa (species and subspecies, including established aliens) in an area of 131,957 km2. The phytogeographic region of north-east Greece (NE), an area characterised by physiographic diversity, is the richest floristic region in Greece, yet it remains less-explored compared to other regions.New informationWe present an annotated inventory of 32 vascular plant taxa collected predominantly from the East Macedonia and Thrace Regions (Makedonia and Thraki, NE Greece), following fieldwork that took place between 2013 and 2021. In our results, we identify seven new additions to the Greek flora (Aethionemasaxatilesubsp.rhodopaeum, Anthericumramosum, Asteramellus, Euphorbiacarniolica, Hesperismatronalissubsp.matronalis, Saponariastranjensis and Silenefetlerii) and 25 new records to the regional Greek flora consisting of mostly rare Balkan endemics of a restricted distribution and presumable conservation priorities. Taxonomic comments are added, where necessary. IUCN threat categories are assigned to certain taxa. Our investigation highlights the importance of the Balkan border areas as a source of new floristic elements for single countries and points to the importance of range-restricted taxa, in addition to single-country endemics, to address plants with a narrow distribution pattern.

Competitive Interactions between Two Non-Native Species (Alliaria petiolata [M. Bieb.] Cavara & Grande and Hesperis matronalis L.) and a Native Species (Ageratina altissima [L.] R.M. King & H. Rob.).

Alliaria petiolata and Hesperis matronalis are wide-ranging non-native species in North America. Ageratina altissima is native to North America but has become a concern as an invasive species in Asia. A replacement series experiment was established to quantify the competitive interactions between these three species and to rank their relative competitiveness with each other. We assessed leaf count, chlorophyll content, and aboveground biomass with comparisons between replacement series mixtures and competition species. Overall leaf count and aboveground biomass were greatest in A. altissima and chlorophyll content was lowest in A. petiolata. Chlorophyll content and aboveground biomass were lower for A. altissima in competition with A. petiolata compared to H. matronalis. Leaf count for A. petiolata was lower in competition with A. altissima compared to H. matronalis. Aboveground biomass for H. matronalis was lower in competition regardless of the species compared to monoculture. There were also negative trends in biomass for A. petiolata in competition with increasing neighbors. However, for A. altissima, the negative trend in biomass was with A. petiolata, H. matronalis did not negatively affect A. altissima biomass. Our rank order of competitiveness was A. altissima > A. petiolata >> H. matronalis.

Wild and Weedy Hesperis Matronalis Hosts Turnip Mosaic Virus Across Heterogeneous Landscapes in Upstate New York

Wild and Weedy Hesperis Matronalis Hosts Turnip Mosaic Virus Across Heterogeneous Landscapes in Upstate New York

Investigation of the glucosinolates in Hesperis matronalis L. and Hesperis laciniata All.: Unveiling 4′-O-β-d-apiofuranosylglucomatronalin

The glucosinolate (GSL) profiles of wild-growing plants from the genus Hesperis, i.e. Hesperis laciniata All. (leaf, stem, flower, and root) from Croatia and Hesperis matronalis L. (leaf, stem, flower, seed, and root) from Canada, were established by LC-MS. During this investigation, 5-(methylsulfanyl)pentyl- (3), 6-(methylsulfanyl)hexyl- (4), 6-(methylsulfinyl)hexyl- (6), and 4′-α-l-rhamnopyranosyloxybenzyl- (17) GSLs were identified. In addition, the presence of 7-(methylsulfinyl)heptyl GSL (18), hydroxy-(α-l-rhamnopyranosyloxy)benzyl GSL, and of one d-apiosylated analogue of 17 were suggested. Moreover, one new GSL, 4′-O-β-d-apiofuranosylglucomatronalin (19) was isolated from H. laciniata (flower, steam and leaf) and characterized by spectroscopic data interpretation. Finally, we report the presence of 3, 4, 6, 19, glucosinalbin (12), and 4-hydroxyglucobrassicin (20) in H. matronalis and hypothesize the presence of glucomatronalin (13) and 3-hydroxy-6-(methylsulfanyl)hexyl GSL (21).

Sozological peculiarities of the flora of the Desna Plateau (Ukraine)

According to the goals and objectives of the Global Strategy for Plant Conservation for 2011–2020 studying and monitoring the rare component of regional floras is highly relevant. This article presents the results of a nature conservation study of the flora of the Desna Plateau (Кrolevets’ and Hlukhiv geobotanic region), situated in the northeast part of Ukraine at the border line of two geomorphological regions (the Central Russian Upland and the Polesia-Dnipro lowland) and of two physical-geographical zones (Novhorod-Sivers`ke Polesia and the Sums`ky forest-steppe. In the orographic aspect, the territory is made up of the western spurs of the Central Russian Upland with the dissectioned forms of the relief. The studied area consists of about 4000 km2. Field route studies aimed at compiling floral lists, geobotanical description and herbarizing were conducted in the period 2002–2016. Separate sites of nature conservation interest were re-examined in different seasonal periods. The basis of the annotated summary of the rare species of vascular plant flora is made up by the materials of original research, as well as herbarium (KW) and literary reviews. The complex floristic analysis of the rare component of the regional flora was carried out in cameral conditions, which made it possible to identify the systematic, geographical, ecological, phytocenological and nature conservation structures of the rare species of vascular plant flora in the investigated area. According to the data received, the total number of species of vascular plants endangered by anthropogenic impact is composed of 85 species belonging to 32 families, 54 genera. Among the families, the following predominate: Orchidaceae (14 genera), Asteraceae, Ranunculaceae (8 genera in each), Iridaceae (5), Aspidiaceae, Cyperaceae (4 genera in each). Among the genera, the following predominate – Carex (4 species); Dryopteris, Orchis, Iris, Jurinea – 3 species in each. It was concluded that among the rare species there are plant species of different physical and geographical zones and this corresponds to the ecotone location of the region. Among species at the edge of their range, 14 are on the extreme northern border of their typical range (Linum flavum L., Carex rhizina Blytt ex Lindb., Centaurea ruthenica Lam., Trinia multicaulis Schishk, Aster amellus L., Galatella linosyris (L.) Rchb. f., Stipa tirsa Steven), 7 species are found on the southern border of their range (Cypripedium calceolus L., Pedicularis sceptrum-carolinum L., Parnassia palustris L., Lycopodium annotinum L., 4 species (Anemone nemorosa L., Salix myrsinifolia Salisb., Galanthus nivalis L., Gladiolus imbricatus L. are found on the eastern border of their distribution. The nature conservation structure includes 4 species from the Bern Convention on the Conservation of European Wildlife and Natural Habitats (Dracocephalum ruyschiana L., Pulsatilla patens (L.) Mill s.l., Salvinia natans L., Ostericum palustre (Bess) Bess); 3 species from the supplement to the CITES Convention (Adonis vernalis L., Cypripedium calceolus L., Orchis militaris L.), 27 species from the Red Book of Ukraine (Lilium martagon L., Allium ursinum L., Neottia nidus-avis (L.) Rich, Stipa pennata L.), 49 species from Sumy region rare species list (Clematis recta L., Pulsatilla patens (L.) Mill., Eremogone saxatilis (L.) Ikonn., Drosera rotundifolia L., Pedicularis kaufmannii Pinzg., Carex limosa L., C. umbrosa Host.). In addition, we suggest including into this list Sanquisorba officinalis L., Briza media L., Beckmania eruciformis (L.) Host., Melica nutans L., Coronilla varia L., Salvia nutans L., Scilla siberica Haw., Corydalis solida (L.) Clairv., Valeriana officinalis L., Vincetoxicum hirundinaria Medic., Menyanthes trifoliata L., Hesperis matronalis L., Naumburgia thyrsiflora (L.) Rchb., Thalictrum aquilegifolium L., T. minus L., Verbascum nigrum L., Polygonatum multiflorum (L.) All. It was found that among the rare species of the region mesophytes predominate and they are confined to meadow and forest habitats. To a lesser extent, xeromesophytes and xerophytes of meadow-steppe, as well as hygrophytes and hydrophytes of shoreline and water habitats are represented. The nature reserve fund of the region was analyzed according to the methodology of assessing the nature reserves by botanical value priority criteria. To optimize the region's nature reserve fund, a rationale for creating a landscape reserve of local significance “Zvenyhorods'ky” with a total area of 2,000 hectares was compiled, giving it the status of a regional biodiversity centre.

Flora of the settlement Lukh of Ivanovo Region

In the article the floristic data of Lukh, one of the ancient Ivanovo region settlements, are given. The present Lukh settlement flora consists of 402 vascular plant species from 4 classes, 76 families and 255 genera. Compositae 53 species (13,2%), Rosaceae 39 species (9,7%), Poaceae 27 species (6,9%), Fabaceae 23 species (5,7%), Cruciferae 18 species (4,2%) are leading families of the flora. The basis of biomorphological structure of the Luch flora is made up by the perennial grass plants (233 species; 57,9%), arboreous plants include 60 species (14,9%), among which 23 species are trees, 33 species are shrubs. Geographical analysis ascertained prevalence of native species. The native component of Lukh flora comprises 247 species (61,6%), the аlien component 153 species (38,4%). The adventivity index is 0,38 which is typical for the small towns and large settlements of the Upper Volga region. 2 rare species (Gerntiana pneumonanthe, Populus nigra) included in the regional red data book, some native (Thelypteris palustris, Juniperus communis, Dactulorhiza fuchii, Platanthera bifolia, Convallaria majalis, Carex pilosa, Trollius earopaeus, Daphne mezerum) and alien (Atriрlex patens, Symphytum x uplandicum, Hesperis matronalis, Veronica persica, Zizania latifolia) rare plant species are briefly characterized.

Invader disruption of belowground plant mutualisms reduces carbon acquisition and alters allocation patterns in a native forest herb.

Invasive plants impose novel selection pressures on naïve mutualistic interactions between native plants and their partners. As most plants critically rely on root fungal symbionts (RFSs) for soil resources, invaders that disrupt plant-RFS mutualisms can significantly depress native plant fitness.Here, we investigate the consequences of RFS mutualism disruption on native plant fitness in a glasshouse experiment with a forest invader that produces known anti-fungal allelochemicals. Over 5months, we regularly applied either green leaves of the allelopathic invader Alliaria petiolata, a nonsystemic fungicide to simulate A. petiolata's effects, or green leaves of nonallelopathic Hesperis matronalis (control) to pots containing the native Maianthemum racemosum and its RFSs. We repeatedly measured M. racemosum physiology and harvested plants periodically to assess carbon allocation. Alliaria petiolata and fungicide treatment effects were indistinguishable: we observed inhibition of the RFS soil hyphal network and significant reductions in M. racemosum physiology (photosynthesis, transpiration and conductance) and allocation (carbon storage, root biomass and asexual reproduction) in both treatments relative to the control. Our findings suggest a general mechanistic hypothesis for local extinction of native species in ecosystems challenged by allelopathicinvaders: RFS mutualism disruption drives carbon stress, subsequent declines in native plant vigor, and, if chronic, declines in RFS-dependent species abundance.

Mutualism-disrupting allelopathic invader drives carbon stress and vital rate decline in a forest perennial herb.

Invasive plants can negatively affect belowground processes and alter soil microbial communities. For native plants that depend on soil resources from root fungal symbionts (RFS), invasion could compromise their resource status and subsequent ability to manufacture and store carbohydrates. Herbaceous perennials that depend on RFS-derived resources dominate eastern North American forest understories. Therefore, we predict that forest invasion by Alliaria petiolata, an allelopathic species that produces chemicals that are toxic to RFS, will diminish plant carbon storage and fitness. Over a single growing season, the loss of RFS could reduce a plant's photosynthetic physiology and carbon storage. If maintained over multiple growing seasons, this could create a condition of carbon stress and declines in plant vital rates. Here we characterize the signals of carbon stress over a short timeframe and explore the long-term consequence of Alliaria invasion using Maianthemum racemosum, an RFS-dependent forest understory perennial. First, in a greenhouse experiment, we treated the soil of potted Maianthemum with fresh leaf tissue from either Alliaria or Hesperis matronalis (control) for a single growing season. Alliaria-treated plants exhibit significant overall reductions in total non-structural carbohydrates and have 17 % less storage carbohydrates relative to controls. Second, we monitored Maianthemum vital rates in paired experimental plots where we either removed emerging Alliaria seedlings each spring or left Alliaria at ambient levels for 7 years. Where Alliaria is removed, Maianthemum size and vital rates improve significantly: flowering probability increases, while the probability of plants regressing to non-flowering stages or entering prolonged dormancy are reduced. Together, our results are consistent with the hypothesis that disruption of a ubiquitous mutualism following species invasion creates symptoms of carbon stress for species dependent on RFS. Disruption of plant-fungal mutualisms may generally contribute to the common, large-scale declines in forest biodiversity observed in the wake of allelopathic invaders.

Flavonol glycosides in the purple flowers of Hesperis matronalis L. (Brassicaceae)

• Two flavonol glycosides were isolated from the purple flowers of Hesperis matronalis .

Diadromus pulchellus in North America: field release against leek moth and new characters to distinguish it from Diadromus subtilicornis, a native diamondback moth parasitoid

We report successful overwintering of Diadromus pulchellus in North America (Ontario) following introduction of this species from Europe to control the leek moth, Acrolepiopsis assectella, a recently established alien species. Field rearing revealed that the native Diadromus subtilicornis emerged only from diamondback moth, Plutella xylostella, whereas D. pulchellus was reared almost exclusively from leek moth. The single D. pulchellus reared from diamondback moth was anticipated because host range studies found this species could develop on both leek moth and diamondback moth in the laboratory, although, it had not been previously reported from diamondback moth in the field in Europe. DNA barcoding of specimens of both Diadromus spp. confirmed their species status and novel morphological characters are presented to distinguish D. pulchellus from D. subtilicornis. In addition, DNA from specimens of D. subtilicornis from Europe clustered with DNA from specimens across Canada, confirming that it is a single Holarctic species. Finally, a new host association for D. subtilicornis is recorded from the dame's rocket moth: Pseudoplutella porrectella.

Acylated cyanidin 3-sambubioside-5-glucosides in the purple flowers of Hesperis matronalis L. (Brassicaceae)

► Two new anthocyanins were isolated from the purple flowers of Hesperis matronalis L. ► Acylated anthocyanins were based on 3-sambubioside-5-glucosides of cyanidin. ► This is the fifth genus including cyanidin 3- p -coumaroylsambubioside-5-malonylglucoside in the Brassicaceae.

Bunias orientalis L. as a natural overwintering host OF Turnip mosaic virus

A virus was isolated, using mechanical inoculation, from hill mustard (<i>Bunias orientalis</i> L.) plants exhibiting yellow mottling and blistering on leaves, which were frequently accompanied by asymmetric leaf narrowing. It systemically infected certain plants from the family Brassicaceae (<i>Brassica rapa</i>, <i>Bunias orientalis</i>, <i>Hesperis matronalis</i>, <i>Sinapis alba</i>) as well as <i>Cleome spinosa</i> and <i>Nicotiana clevelandii</i>, and locally <i>Atriplex hortensis</i>, <i>Chenopodium quinoa</i>, <i>Ch. amaranticolor</i>, <i>N. tabacum</i>. In the sap, it maintained infectivity for 3-4 days and lost it after heating for 10 min. at a temperature of 55 - 60<sup>o</sup>C or when diluted with water at 10<sup>-3</sup>. Virus particles were thread- like with a length of 675 - 710 nm. Based on an analysis of biological properties of the pathogen, serological response, particle morphology and data from field observations, it was identified as an isolate of <i>Turnip mosaic virus</i> (TuMV), and hill mustard was recognised as a natural overwintering host for this pathogen.

Tallgrass prairie restoration in the midwestern and eastern United States: a hands-on guide

Tallgrass prairie restoration in the midwestern and eastern United States: a hands-on guide

Antibacterial activity of traditional medicinal plants used by Haudenosaunee peoples of New York State

BackgroundThe evolution and spread of antibiotic resistance, as well as the evolution of new strains of disease causing agents, is of great concern to the global health community. Our ability to effectively treat disease is dependent on the development of new pharmaceuticals, and one potential source of novel drugs is traditional medicine. This study explores the antibacterial properties of plants used in Haudenosaunee traditional medicine. We tested the hypothesis that extracts from Haudenosaunee medicinal plants used to treat symptoms often caused by bacterial infection would show antibacterial properties in laboratory assays, and that these extracts would be more effective against moderately virulent bacteria than less virulent bacteria.MethodsAfter identification and harvesting, a total of 57 different aqueous extractions were made from 15 plant species. Nine plant species were used in Haudenosaunee medicines and six plant species, of which three are native to the region and three are introduced, were not used in traditional medicine. Antibacterial activity against mostly avirulent (Escherichia coli, Streptococcus lactis) and moderately virulent (Salmonella typhimurium, Staphylococcus aureus) microbes was inferred through replicate disc diffusion assays; and observed and statistically predicted MIC values were determined through replicate serial dilution assays.ResultsAlthough there was not complete concordance between the traditional use of Haudenosaunee medicinal plants and antibacterial activity, our data support the hypothesis that the selection and use of these plants to treat disease was not random. In particular, four plant species exhibited antimicrobial properties as expected (Achillea millefolium, Ipomoea pandurata, Hieracium pilosella, and Solidago canadensis), with particularly strong effectiveness against S. typhimurium. In addition, extractions from two of the introduced species (Hesperis matronalis and Rosa multiflora) were effective against this pathogen.ConclusionsOur data suggest that further screening of plants used in traditional Haudenosaunee medicine is warranted, and we put forward several species for further investigation of activity against S. typhimurium (A. millefolium, H. matronalis, I. pandurata, H. pilosella, R. multiflora, S. canadensis).