Intraocular lenses (IOL) should remain in the eye for life after implantation into the capsular bag during cataract surgery. The material must meet various requirements. It is crucial that the material has the best biocompatibility, and it should be flexible and soft for best possible implantation process but also sufficiently stable and stiff for good centering in the eye and posterior capsule opacification prevention. In this laboratory experiment, we used nano-indentation for the mechanical assessment of three hydrophobic acrylic (A, B, C), three hydrophilic acrylic (D, E, F), and one silicone (G) intraocular lens. We wanted to determine whether some react more sensitively to touching/handling than others. The indentation elastic modulus and the creep were obtained from the force displacement curve. For measuring penetration depth and testing of possible damage to the intraocular lenses, the samples were measured at room temperature. A 200-µm-diameter ruby spherical tipped indenter was used for all the tests. Indentations were made to three different maximum loads, namely 5mN (milli Newton), 15mN, and 30mN and repeated three times. The lowest penetration depth (12µm) was observed with IOL B. However, IOL A, D, and F showed similar low penetration depths (20, 18, and 23µm, respectively). Lenses C and E showed slightly higher penetration depths of 36 and 39µm, respectively. The silicone lens (G) showed the greatest penetration depth of 54.6µm at a maximum load of 5mN. With higher maximal loads (15 and 30mN) the penetration depth increased significantly. Lens C, however, showed the same results at both 15 and 30mN with no increase of penetration depth. This seems to fit well with the material and manufacturing process of the lens (lathe-cut). During the holding time of 30s at constant force all six acrylic lenses showed a significant increase of the creep (CIT 21-43%). Lens G showed the smallest creep with 14%. The mean indentation modulus (EIT) values ranged from 1 to 37MPa. IOL B had the largest EIT of 37MPa, which could be caused by the low water content. It was found that results correlate very well with the water content of the material in the first place. The manufacturing process (molded versus lathe-cut) seems to play another important role. Since all included acrylic lenses are very similar, it was not surprising that the measured differences are marginal. Even though hydrophobic materials with lower water content showed higher relative stiffness, penetration and defects can also occur with these. The surgeon and scrub nurse should always be aware that macroscopic changes are difficult to detect but that defects could theoretically lead to clinical effects. The principle of not touching the center of the IOL optic at any time should be taken seriously.