Background: India is a developing country with a rapid transformation in lifestyle including inappropriate diet and inadequate physical activity. More the physical activity greater will be the muscle strength. Handgrip strength is an indicator of muscle strength. Aims and Objectives: This study was conducted to compare handgrip strength among sedentary and active workers and to determine the relationship between body mass index (BMI) and handgrip strength. Materials and Methods: This cross-sectional study was conducted in Dakshina Kannada, Karnataka, India. 50 sedentary subjects and 50 constructive site active laborers were selected as study groups by convenience sampling. Anthropometric parameters such as height and weight, were recorded. Handgrip strength was recorded using a standard adjustable handgrip dynamometer. Handgrip strength was compared between the groups using independent t-test and Pearsons correlation was carried out to find relationship between anthropometric variables and handgrip strength. Results: We noted extremely statistically significant stronger handgrip strength in both the right and left hand of the active group compared to that of the sedentary group (P < 0.0001). There was no significant relationship between BMI and handgrip strength. There was a positive correlation with weight and handgrip strength of active group. Conclusion: The physically active group had higher handgrip strength compared to sedentary group. The sedentary group had significantly higher weight when compared to that of active group which provides evidence that low physical activity is related to weight gain and its complications. The above result emphasizes the need to indulge in regular physical activities.