Dairy Operations Research Articles

Association of dystocia and metritis with daily activity patterns of Holstein dairy cows

Calving related losses due to dystocia and metritis affect the bottom line of dairy operations, and their underlying causes should be investigated to develop preventive procedures with consideration given to animal welfare and farm profitability. Monitoring cow activity patterns around the clock may allow dairy producers and their veterinarians to improve the identification of cows at risk of devleoping dystocia or metritis. The objectives of this study were to assess the effect of dystocia on cow activity behavior 4 days before calving and to assess the effect of metritis on cow activity behavior 3 days before and after diagnosis.

Impact of stockmanship training on dairy farms

Our objectives were to study the stockmanship (low stress cattle handling) knowledge, skills, and behaviours of workers on larger dairy operations and to evaluate the impact on cattle flow and on cattle handling skills.

Effect of milk cessation method at dry-off on behavioral activity of dairy cows

Drying cows off at the end of lactation is a routine management practice in dairy operations. Most dairies in the United States and many other countries dry cows off abruptly (e.g., stop milking cows on a set day), which has been shown to affect cow comfort. Gradually reducing milk production is another approach to dry cows off, routinely used in some countries and herds. The objective of the study was to evaluate the effects of abrupt and gradual milk cessation and milk yield at the time on cow activity after dry-off. Daily lying time, number of lying bouts per day, average lying bout length, and steps taken per day by abruptly and gradually dried-off cows were monitored by data loggers for 2 wk before and after the final milking at the end of lactation. Gradual cows were milked once daily for the last week of lactation, and abrupt cows were milked as usual (3×/d) until the end of lactation. Gradual cessation of milking significantly reduced milk yield by the day of dry-off. After dry-off, gradual cows tended to have longer lying bouts than abrupt cows, but no other differences in cow activity between the 2 treatments were observed. Regardless of the dry-off method, the average length of a lying bout decreased by 4 min and total daily lying time decreased by 19 min after dry-off for each 5-kg increase in milk yield before dry-off. Lying behavior of primiparous cows was more affected by the level of milk yield at dry-off than that of older cows. A reduction in lying times with increasing milk yield may indicate discomfort due to the accumulating milk in the udder. Using a method that lowers milk production before dry-off and managing primiparous and multiparous cows separately around dry-off are beneficial for cow comfort after dry-off.

Cross-sectional study to identify staphylococcal species isolated from teat and inguinal skin of different-aged dairy heifers

The purpose of this study was to describe the prevalence and distribution of staphylococcal species on the teat and inguinal skin of dairy heifers across the various stages of the heifer life cycle. The cross-sectional study included 106 Holstein heifers with an age range of 0 d to 27 mo that were selected from 11 different groups, based on housing type and age, on a single dairy operation. A composite swabbing sample including all 4 teats and a second composite sample including both inguinal regions of each heifer were collected using gas-sterilized electrostatic dusters (Swiffers; Procter and Gamble, Cincinnati, OH). Swabbing samples were mixed with 10 mL of sterile saline, agitated, and cultured on mannitol salt agar plates. At 24 h, plates were read and up to 10 staphylococcal colonies were saved for further analysis. Staphylococcal isolates were speciated using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry or PCR amplification and partial sequencing of rpoB or tuf. The prevalence of staphylococci was compared between the inguinal and teat regions using the chi-squared or Fisher's exact test, as applicable. Logistic regression models were used to investigate the relationship between a heifer's age (treated as a quantitative continuous variable) and the probability of isolating a given staphylococcal species from a given body site (inguinal region or teats). Overall, the most common species identified were Staphylococcus haemolyticus followed by Staphylococcus chromogenes, Staphylococcus xylosus, Staphylococcus devriesei, and Staphylococcus sciuri. Staphylococcus aureus was more prevalent on the teat than in the inguinal region, whereas Staphylococcus arlettae was more prevalent in the inguinal region than on the teat. All other staphylococcal species were as likely to be found on the teat skin as the inguinal region skin. Isolation from the inguinal and teat skin was associated with age for Staphylococcus agnetis, S. chromogenes, S. devriesei, Staphylococcus equorum, S. haemolyticus, Staphylococcus lentus, S. sciuri, Staphylococcus vitulinus, and S. xylosus. The probability of finding S. chromogenes and S. agnetis on the teat and inguinal region increased with age, whereas the probability of S. devriesei and S. haemolyticus decreased with age. This study provides further insight into the ecology of staphylococcal species involved in heifer mastitis.

Assessing climate vulnerabilities and adaptive strategies for resilient beef and dairy operations in the tropics

Cattle ranchers and dairy farmers operating throughout many tropical regions are experiencing major challenges associated with climate change such as higher incidence of heat stress and drought. These effects can result in reduced productivity of rangeland, shortage of nutritional feed, increased heat stress on animals, and high energy costs for cooling. High temperatures and resultant heat stress reduce animal productivity and increase the proliferation and survival of parasites and disease pathogens. Warming reduces the ability of dairy cattle to produce milk and gain weight and can also lower conception rates. This paper reviews research from the Caribbean on heat tolerant traits in bovine and presents evidence that introducing a “slick hair” gene into Holstein cows by crossbreeding with Senepols may increase thermo-tolerance and productivity. As in other parts of the tropics, principal cattle breeds in Puerto Rico and the US Virgin Islands have been largely introduced from temperate regions. Research indicates these animals may be poorly adapted to rising temperatures, leaving them increasingly vulnerable to chronic heat stress and reduced productivity. Adaptive practices have been developed in breeding and pasture management programs including selection for more heat-resistant genotypes, silvopasturing and crop diversification in forage production, and optimizing facilities and practices to reduce heat stress. Given the nature of climate vulnerability, an integrated approach to adaptation will likely have the greatest success in reducing future risk for producers.

Evaluation of the Decontamination Power of Aqueous and Gaseous Ozone on Various Contaminated Surfaces with Cattle Manure containing Salmonella

Despite using ozone (O3) as an attractive alternative disinfectant for more than a century, little is known about the killing capacity of O3 against Salmonella contaminated different surfaces in dairy operations. Accordingly, our objective was to characterize the killing capacity of aqueous and gaseous O3 at different operational conditions on Salmonella Typhimurium and Salmonella Choleraesuis (aSTC) contaminated plastic, metal, nylon, rubber, and wood surfaces. In a crossover design, 14 strips of each material were randomly assigned between 3 groups, treatment (n=6), positive-control (n=6), and negative-control (n=2). The strips were loaded with aSTC (107-108) and exposed to aqueous O3 of 2, 4, and 9 ppm for 4 minutes, and gaseous O3 of 1 and 9 ppm for 30, 60, and 120 minutes. Plastic and metal surfaces were most effectively decontaminated, an aqueous O3 of 4 ppm reduced aSTC by 6.7 and 5.2- log10, respectively, and 9 ppm resulted in no detectable aSTC. On nylon and rubber, aqueous O3 of 9 ppm reduced aSTC population to a safe level (5.8 and 5.1-log10). On wood, both aqueous and gaseous O3 at up to 9 ppm were unable to reduce aSTC to undetectable limit. Interestingly, aSTC load and sequential washing showed same impacts on the reduction rate of aSTC on complex surfaces. Our findings strongly indicate that aqueous O3 of 9 ppm for 4 minutes exposure is an effective means to clear smooth surfaces of a high Salmonella load. However, sequential washing or decrease the bacterial load is needed to effectively decontaminate complex surfaces.

Prediction of Human Responses to Dairy Odor Using an Electronic Nose and Neural Networks

Abstract. Odor emitted from dairy operations may cause negative reactions by farm neighbors. Identification and evaluation of such malodors is vital for better understanding of human response and methods for mitigating effects of odors. The human nose is a valuable tool for odor assessment, but using human panels can be costly and time-consuming, and human evaluation of odor is subjective. Sensing devices, such as an electronic nose, have been widely used to measure volatile emissions from different materials. The challenge, though, is connecting human assessment of odors with the quantitative measurements from instruments. In this work, a prediction system was designed and developed to use instruments to predict human assessment of odors from common dairy operations. The model targets are the human responses to odor samples evaluated using a general pleasantness scale ranging from -11 (extremely unpleasant) to +11 (extremely pleasant). The model inputs were the electronic nose measurements. Three different neural networks, a Levenberg-Marquardt back-propagation neural network (LMBNN), a scaled conjugate gradient back-propagation neural network (CGBNN), and a resilient back-propagation neural network (RPBNN), were applied to connect these two sources of information (human assessments and instrument measurements). The results showed that the LMBNN model can predict human assessments with accuracy as high as 78% within a 10% range and as high as 63% within a 5% range of the targets in independent validation. In addition, the LMBNN model performed with the best stability in both training and independent validation. Keywords: Animal production, Hedonic tone, Olfactometric models.

The effects of periparturient events, mastitis, lameness and ketosis on reproductive performance of Holstein cows in a hot environment

The aim of this study was to determine the association among periparturient events such as mastitis, lameness (infectious and non-infectious) and ketosis and the reproductive performance of high producing Holstein cows in a hot environment. The health status and reproductive data variables were recorded from 6,566 completed lactations in a large dairy operation. A logistic regression model with the stepwise procedure was used, considering the occurrence of the health problems during the pre-breeding period as fixed effect risk factors and reproductive variables as dependent variables. Pregnancy rate of cows contracting clinical mastitis (CM) around the period of first inseminations was five percentage points lower than cows without this disease. Cows with CM shortly before or after the first service had twice the risk of requiring more than three services per pregnancy than cows with healthy udders. Cows experiencing abortion, laminitis or metritis were two times more likely to require >3 services per pregnancy than cows not experiencing these reproductive disorders. Cows experiencing lameness, mastitis and metritis presented a significantly longer interval between calving and pregnancy compared to healthy cows. Ketosis and lameness increased the odds of a cow of being inseminated for the first time after 70 days in milk. In conclusion, these results suggest that abortion, ketosis, CM, retained placenta (RP), metritis, and lameness are associated with decreased reproductive performance in high producing Holstein cows subjected to thermal stress.

Prevalence of Salmonella enterica, Listeria monocytogenes, and pathogenic Escherichia coli in bulk tank milk and milk filters from US dairy operations in the National Animal Health Monitoring System Dairy 2014 study

The dairy farm environment is a well-documented reservoir for zoonotic pathogens such as Salmonella enterica, Shiga-toxigenic Escherichia coli, and Listeria monocytogenes, and humans may be exposed to these pathogens via consumption of unpasteurized milk and dairy products. As part of the National Animal Health Monitoring System Dairy 2014 study, bulk tank milk (BTM, n = 234) and milk filters (n = 254) were collected from a total of 234 dairy operations in 17 major dairy states and analyzed for the presence of these pathogens. The invA gene was detected in samples from 18.5% of operations and Salmonella enterica was isolated from 18.0% of operations. Salmonella Dublin was detected in 0.7% of operations. Sixteen Salmonella serotypes were isolated, and the most common serotypes were Cerro, Montevideo, and Newport. Representative Salmonella isolates (n = 137) were tested against a panel of 14 antimicrobials. Most (85%) were pansusceptible; the remaining were resistant to 1 to 9 antimicrobials, and within the resistant strains the most common profile was resistance to ampicillin/clavulanic acid, ampicillin, cefoxitin, ceftiofur, ceftriaxone, chloramphenicol, streptomycin, sulfisoxazole, and tetracycline. Listeria spp. were isolated from 19.9% of operations, and L. monocytogenes was isolated from 3.0% of operations. Serogroups 1/2a and 1/2b were the most common, followed by 4b and 4a. One or more E. coli virulence genes were detected in the BTM from 30.5% of operations and in the filters from 75.3% of operations. A combination of stx2, eaeA, and γ-tir genes was detected in the BTM from 0.5% of operations and in the filters from 6.6% of operations. The results of this study indicate an appreciable prevalence of bacterial pathogens in BTM and filters, including serovars known to infect humans.

Water Use and Conservation on a Free-Stall Dairy Farm

Livestock watering can represent as much as 20% of total agricultural water use in areas with intensive dairy farming. Due to an increased emphasis on water conservation for the agricultural sector, it is important to understand the current patterns of on-farm water use. This study utilized in situ water meters to measure the year-round on-farm pumped water (i.e., blue water) on a ~419 lactating cow confined dairy operation in Eastern Ontario, Canada. The average total water use for the farm was 90,253 ± 15,203 L day−1 and 33,032 m3 annually. Water use was divided into nutritional water (68%), parlour cleaning and operation (14%), milk pre-cooling (15%), barn cleaning, misters and other uses (3%). There was a positive correlation between total monthly water consumption (i.e., nutritional water) and average monthly temperature for lactating cows, heifers, and calves (R2 = 0.69, 0.84, and 0.85, respectively). The blue water footprint scaled by milk production was 6.19 L kg−1 milk or 6.41 L kg−1 fat-and-protein corrected milk (FPCM) including contributions from all animal groups and 5.34 L kg−1 milk (5.54 L kg−1 FPCM) when excluding the water consumption of non-lactating animals. By applying theoretical water conservation scenarios we show that a combination of strategies (air temperature reduction, complete recycling of milk-cooling water, and modified cow preparation protocol) could achieve a savings of 6229 m3 annually, a ~19% reduction in the total annual water use.

A large outbreak of bovine botulism possibly linked to a massive contamination of grass silage by type D/CClostridium botulinumspores on a farm with dairy and poultry operations

Type D bovine botulism outbreaks associated with poultry litter are increasingly reported in European countries, but the circumstances of exposure to Clostridium botulinum toxins remain unclear. In spring 2015, a large type D/C bovine botulism outbreak affected a farm with dairy and poultry operations. Epidemiological and laboratory investigations strongly suggest that the outbreak was caused by feeding cattle with insufficiently acidified grass silage that was contaminated by type D/C C. botulinum spores. The source of the spores remains unclear, but could have been a stack of poultry litter stored in the grass silage pasture before harvesting. The presence of putrefied poultry carcasses mixed in with the litter is relatively unlikely considering the careful daily removal of poultry carcasses. These findings reinforce the importance of proper ensiling of feed materials and highlight the need for safe disposal of poultry litter, even in the case of good management of poultry deadstock, in order to prevent bovine botulism.

Risk assessment modelling of fecal shedding caused by extended-spectrum cephalosporin-resistant Escherichia coli transmitted through waste milk fed to dairy pre-weaned calves

Waste milk feeding is a common practice in dairy operations. Regardless of the benefits of this practice to the dairy farmers, concerns from the potential dissemination of antimicrobial-resistant bacteria through the gut and subsequent shedding by calves into the environment are increasing. In this study, we employed Monte Carlo simulation to assess the risk of shedding extended-spectrum cephalosporin-resistant Escherichia coli (ESC-R E. coli) caused by waste milk feeding in pre-weaned calves using an exponential dose-response model fit to data for E. coli O157:H7 in cattle. Data from pertinent studies were included in our model to predict the risk of shedding. The median (5th and 95th percentiles) for the daily risk of shedding ESC-R E. coli by calves fed only contaminated waste milk was predicted to be 2.9 × 10-3 (2.1 × 10-3, 3.7 × 10-3), representing a median daily risk of 29 out of 10,000 calves shedding ESC-R E. coli due to exclusive feeding of waste milk containing ESC-R E. coli. This median value was reduced by 94% when accounting for the proportion of waste milk that does not contain ESC-R E. coli. The overall risk of shedding ESC-R E. coli through the pre-weaning period for farms that feed waste milk to calves was 5.7 × 10-3 (2.4 × 10-3, 1.1 × 10-2), representing 57 out of 10,000 calves. When accounting for the proportion of farms that do not feed waste milk, the pre-weaning period risk was reduced by 23%. By varying the prevalence of ESC-R E. coli in waste milk using values of 3, 1.5, and 1%, the daily risk of shedding decreased by factors of 50, 65, and 82%, respectively, which supports the reduction of contamination or discontinuation of feeding waste milk containing ESC-R E. coli as major mitigation measures to reduce the risk of shedding caused by ingestion of resistant bacteria. It is anticipated that the effects of antimicrobial residues in waste milk, which was not considered herein due to lack of data, would further increase risks. Although waste milk feeding to calves may be economically beneficial to the dairy farmers, there exists the risk of dissemination of ESC-resistant bacteria into the environment.

Impact of age on antibiotic distribution and efficacy in calves

Calfhood diseases have a major economic impact on beef and dairy operations, due to the costs associated with mortality, treatment, and the long-term effects on growth and performance. With very limited approved drugs for use in "pre-ruminant” calves and heifers, questions remain about the effectiveness of these drugs in young animals. It is important to prevent unnecessary use of antibiotics in calves to limit the development of resistant bacteria and residues, as well as take into account the developmental changes that occur in neonatal calves that would affect the distribution, therefore efficacy of selected drug therapy. The purpose of this article will give an overview of the effects of age on absorption, metabolism, distribution, and elimination of commonly administered drugs and its impact on efficacy in different ages of calves.

Salmonella Heidelberg

Salmonella is endemic in modern dairy operations. Colonization of newborn calves typically occurs at birth although in-utero transmission has been reported. Most of the time when newborn calves are colonized with Salmonella spp., there are no issues with calf health with the caveat that the calves receive an adequate quantity of good quality colostrum within 2 hours of birth, they receive at least 1.5 gallons of milk or its equivalent in milk replacer each day, there is adequate space and heat or cold stress is minimized. However, not all Salmonella behave the same way in newborn calves. This report describes a very dangerous, multi-drug resistant (MDR) strain of Salmonella Heidelberg that can cause high mortality (> 25%) in well managed dairy operations. In addition, this same strain of Salmonella has a high potential to cause illness and hospitalization of calf care workers as well.

Life cycle assessment of a detailed dairy processing model and recommendations for the allocation to single products

PURPOSE: This study analyses the environmental impacts referring to dairy products and to the operation of a dairy. The study aims to better understand different process stages in a dairy operation. This analysis can be used to improve the flows of energy, water, and materials in the dairy operation. The results are also used to suggest an improved allocation model for assigning the impacts of operation to single dairy products. METHODS: The analysis is based on a detailed, product-specific model calculation for the use of energy, water, and chemicals for more than 40 subprocesses of a dairy operation. This model has been used to elaborate the life cycle inventory for a detailed life cycle assessment study. The environmental impacts are analyzed from cradle to gate including and excluding the raw milk input. The environmental impacts are assessed with the midpoint indicators suggested by the International Reference Life Cycle Data System. Finally, results of this study are compared with an allocation model recommended for life cycle assessment (LCA) studies on milk products. RESULTS AND DISCUSSION: The analysis of the model dairy shows that raw milk production has the main impact in all categories. Consumer packaging has the second biggest impact in many categories. The detailed dairy processing model allows the assignment of inputs and outputs for each subprocess to single dairy products and thus avoids allocation largely. The analysis of inputs to different dairy products per kilogram shows that ultra-high-temperature (UHT)-processed milk uses more chemicals for cleaning compared to the other products. Cream uses more electricity and heat compared to UHT milk and to yogurt. CONCLUSIONS: A detailed discussion shows the overlaps and differences found for the allocation of inputs to the milk processing to final dairy products. Allocation models for different types of inputs are partly confirmed by the detailed theoretical model used for this LCA. The allocation of chemicals, steam, and electricity to single products can be improved based on the detailed dairy model developed in this study.

Dairy calf management—A comparison of practices and producer attitudes among conventional and organic herds

Dairy calves are at high risk for morbidity and mortality early in life. Understanding producer attitudes is important for implementation of best management practices to improve calf health. The objectives of this study were to evaluate usage frequency and producer attitudes on key calf management practices between conventional and organic dairy operations. A cross-sectional survey was mailed to conventional and organic dairy producers in Ohio and Michigan that included questions on cow-calf separation, colostrum management, and vaccination use. The overall survey response rate was 49% (727/1,488); 449 and 172 conventional and organic producer respondents, respectively, were included in the final analysis. Binary, cumulative, and multinomial logistic regression models were used to test differences within and between herd types for management practices and producer attitudes. The majority of conventional (64%, 279/439) producers reported separating the calf from the dam 30 min to 6 h after birth. More organic (34%, 56/166) than conventional (18%, 80/439) producers reported separation 6 to 12 h after birth, and organic producers were more likely to agree time before separation is beneficial. Few conventional (10%, 44/448) and organic (3%, 5/171) producers reported measuring colostrum quality. Most conventional producers (68%, 304/448) hand-fed the first feeding of colostrum, whereas the majority of organic producers (38%, 69/171) allowed calves to nurse colostrum. Last, 44% (188/430) of conventional producers reported vaccinating their calves for respiratory disease, compared with 14% (22/162) of organic producers; organic producers were more likely to perceive vaccines as ineffective and harmful to calf health. Thus, the usage frequency and perceived risks and benefits of calf management practices vary considerably between conventional and organic dairy producers. These findings provide helpful information to understand decision making at the herd level regarding key calf management and health practices, regardless of production systems.

The effect of tail paint formulation and heifer behavior on estrus detection

Although heifers can have better conception rates than cows, they are still subject to poor estrus detection and economic losses from reduced reproductive efficiency. Tail paint has been successful in identifying estrus, but behaviors such a licking or rubbing have been believed to remove the paint and lead to false-positives. To investigate tail paint utilization and potential relationships among behaviors, eighteen Holstein heifers were randomly assigned to one of three treatments: a control tail chalk (CON), tail chalk with proprietary ingredient (CHALK+); and a spray formulation (SPRAY). Experimental design was a replicated 3×3 Latin square. Visual observations were performed in 30min segments every 2h from 6AM to 6PM. Ovaries were examined via ultrasound imaging on d 0, 7, and 9 of each period. The presence of follicles or a corpus luteum (CL) was recorded with their respective sizes. Heifers receiving SPRAY had a lower number of licks received per day and less tail paint removed regardless of day or follicle size when compared with CON or CHALK+. Rump lick received, chin rest received, anogenital sniff received, mount received, and both initiated and received behaviors for attempt to mount occurred more in heifers with large follicles regardless of day. Producers looking for heifers to breed should focus on those receiving rump lick, chin resting, anogenital sniff, mount, and attempt to mount. The use and combination of these estrus detection tools can improve reproductive efficiency in dairy operations.

Dairy Productivity and Technical Change: An Analysis of Confinement and Management Intensive Grazing Dairies in Maryland for 1995–2009

U.S. dairy production has been consolidating into large-scale confinement operations. Large numbers of small- to medium-scale dairies have disappeared in the last two decades, and many more are disappearing. This article analyzes small- to medium-scale dairy operations in Maryland during 1995–2009 for changes in technology and efficiency through a novel two-stage DEA approach to examine productivity changes. Conventional confinement dairy operations and management-intensive grazing dairies are analyzed separately. The results show that both dairy systems have become more productive on the technological frontiers, yet the rate of technical change for graziers was less than half the rate for confinement.

Changes in animal performance and profitability of Holstein dairy operations after introduction of crossbreeding with Montbéliarde, Normande, and Scandinavian Red

An individual-based mechanistic, stochastic, and dynamic simulation model was developed to assess economic effects resulting from changes in performance for milk yield and solid contents, reproduction, health, and replacement, induced by the introduction of crossbreeding in Holstein dairy operations. Three crossbreeding schemes, Holstein × Montbéliarde, Holstein × Montbéliarde × Normande, and Holstein × Montbéliarde × Scandinavian Red, were implemented in Holstein dairy operations and compared with Holstein pure breeding. Sires were selected based on their estimated breeding value for milk. Two initial operations were simulated according to the prevalence (average or high) of reproductive and health disorders in the lactating herd. Evolution of operations was simulated during 15 yr under 2 alternative managerial goals (constant number of cows or constant volume of milk sold). After 15 yr, breed percentages reached equilibrium for the 2-breed but not for the 3-breed schemes. After 5 yr of simulation, all 3 crossbreeding schemes reduced average milk yield per cow-year compared with the pure Holstein scheme. Changes in other animal performance (milk solid contents, reproduction, udder health, and longevity) were always in favor of crossbreeding schemes. Under an objective of constant number of cows, margin over variable costs in average discounted value over the 15 yr of simulation was slightly increased by crossbreeding schemes, with an average prevalence of disorders up to €32/cow-year. In operations with a high prevalence of disorders, crossbreeding schemes increased the margin over variable costs up to €91/cow-year. Under an objective of constant volume of milk sold, crossbreeding schemes improved margin over variable costs up to €10/1,000L (corresponding to around €96/cow-year) for average prevalence of disorders, and up to €13/1,000L (corresponding to around €117/cow-year) for high prevalence of disorders. Under an objective of constant number of cows, an unfavorable pricing context (milk price vs. concentrates price) increased slightly crossbreeding positive effects on margin over variable costs. Under an objective of constant volume of milk, only very limited changes in differences of margins were found between the breeding schemes. Our results, obtained conditionally to the parameterization values used here, suggest that dairy crossbreeding should be considered as a relevant option for Holstein dairy operations with a production level until 9,000 kg/cow-year in France, and possibly in other countries.

266 Prevalence and risk factors for antimicrobial resistance on U.S. dairy operations

Antimicrobial resistance has become a major public health concern, and animal agriculture is often implicated as a source of resistant bacteria. The primary objective of this study was to determine prevalence of antimicrobial resistance in Salmonella and E. coli from healthy animals on dairy farms in Pennsylvania. Confidence intervals (CI) of farm prevalence were calculated for resistance, and the comparisons between different animal groups were adjusted for within-farm clustering. The secondary objective was to identify potential risk factors associated with antimicrobial resistance. In particular, we investigated the correlations between previous antimicrobial use and current resistance in E. coli using multiple logistic regression models. A total of 444 composite manure samples were collected from 80 herds in Pennsylvania. Four age and production groups were included: pre-weaned calves, post-weaned calves, dry cows, and lactating cows. E. coli (n = 2370) and Salmonella (n = 1095), when isolated from the manure composite samples (n = 444), were screened for resistance on antimicrobial supplemented agar. Isolates of interest were further assayed against 14 antimicrobials using a broth microdilution method. Salmonella was isolated from at least one composite manure sample from 51 (67%) farms, with 99% being pan-susceptible, and was detected more frequently in lactating cows and dry cows than pre- and post-weaned calves. The most prevalent Salmonella serogroups were K, C1, and C2. Among the E. coli isolates, resistance was more frequently detected in pre-weaned and post-weaned calves than in dry and lactating cows for all 14 antimicrobials tested (P < 0.05). Multi-drug resistance (resistant to ≥3 antimicrobial classes) in E. coli was detected in 66 farms (83%) and was detected more often in pre-weaned calves (P < 0.05). The blaCTX-M and blaCMY genes were found in cephalosporin-resistant E. coli from 5% and 35% of the farms surveyed, respectively. A number of risk factors, including antimicrobial use, were found to be associated with an increased likelihood of resistance in E. coli, and these associations were not limited to the same antimicrobial classes. The results of this study showed dairy farms, especially pre-weaned calves and their environment, are significant reservoirs for antimicrobial resistance, and antimicrobial use may aggravate the resistance problem.