Saanen is a specialized dairy goat breed originating from Switzerland, imported and encouraged to be raised in Vietnam. This article presents the results of a survey of estradiol and progesterone levels during pregnancy in Saanen goats at the Binh Duong Large Livestock Research and Development Center. After monitoring mating, goats were sampled for blood every 7 days until birth, at 6-8 am. Hormone levels were determined by electrochemiluminescence assay, with the Cobas E601 immunoassay system. The results showed that after 14 days of conception, progesterone levels gradually increased (3.21±0.36 ng/ml) and shifted to higher levels, peaking on day 70 of pregnancy (12.28±0.52 ng/ml) (in the 1-2 years old group); corresponding values were 3.18±0.75-11.88±0.92 ng/ml (in the 3-4 years old group). The high value range was maintained throughout pregnancy and decreased rapidly in the 14-21 days before birth in both age groups. Estradiol, maintained at low concentrations from 0.73±0.35 to 2.65±1.25 pg/ml (in the 1-2 years old group); from 0.98±0.48 to 3.27±1.95 pg/ml (in the 3-4 years old group) in the first 21 days of pregnancy and then gradually increased to above 10 pg/ml (in both age groups) on day 28. Estradiol continued to increase and reached a maximum level (205.48±32.81 pg/ml) in group 1 on day 126 and had an average value below 10 pg/ml within 1-2 days after giving birth. The results of this study help diagnose pregnancy, predict gestational age for effective reproductive management, and serve as a reliable reference when using assisted reproductive tools and strategies to improve reproductive performance in goats.