Wisconsin Dairy Farms Research Articles

Milk Income over Feed Cost Margin, Margin Protection Program, and Farm Finances for a Sample of Wisconsin Dairies in 2000–2017

AbstractThe relationship between farm‐level milk income over feed cost (IOFC) margin and national margin, profit risk, and terminal stress risk are investigated using data on Wisconsin dairy farms over the period 2000–2017. Results show that farm‐specific IOFC margins were not highly correlated with the national margin, higher farm IOFC margin increased profit level and volatility but decreased downside risk, and farm terminal stress appears unaffected by either the margin or the related payments. These findings suggest that, for sample farms, a policy that targets IOFC margin might reduce downside risk of net farm income.

One Health: Farmworker Perceptions of Antibiotic Resistance and Personal Protective Practices on Wisconsin Dairy Farms

Background: Antimicrobials are used on dairy farms for preventing disease and treating common infections such as mastitis. Objective: We aimed to understand farmworker practices that potentially contribute to transmission of antimicrobial resistance bacteria and their genes (ARG) among animals and farm workers, and to identify human behavioral interventions to reduce risk. Methods: Focus groups with farm workers were held at 8 dairy farms across Wisconsin selected to represent a range of antibiotic use in cattle. We explored the nature of potentially high-risk practices and farm-worker knowledge and experiences with antibiotic use and resistance. Farm workers were asked to describe common tasks, including hand hygiene and eating practices, and the policies guiding these practices. Focus groups were conducted in English and Spanish guided by the Systems Engineering in Patient Safety (SEIPS) framework, adapted for an agricultural context. Discussions were recorded, transcribed, and translated. A content analysis was conducted to identify themes. Dedoose version 8.0.35 software was used to organize the data. Results: In total, 10 focus groups were conducted on 8 farms. Knowledge of when to use antibiotics for human health varied; upset stomach, headache, and flu symptoms were suggested as appropriate uses. Few workers had personal experience with antibiotic resistance at home or on the farm. Some displayed knowledge of the role of antibiotic stewardship in preventing the spread of ARG (“I guess all dairy farmers have a responsibility not to overdo it”). Others associated the risk of spread with the consumption of raw milk or meat from cows receiving antibiotics. Knowledge of personal protective equipment was stronger among workers who commonly reported glove use. Some perceived glove use to be mandatory, and others chose to wear gloves in the perceived absence of written rules. Some workers reported changing gloves numerous times throughout the day, and others did so less frequently or “only when they rip.” In general, hand hygiene practices are guided by individual knowledge of established rules, beliefs about risk, and personal discretion. Conclusions: Knowledge about mechanisms of spread of ARGs varies among workers on Wisconsin dairy farms and reflects a combination of farm-level rules, experience, individual knowledge, and beliefs. Applying knowledge from the healthcare setting to reduce ARG spread into agriculture is crucial to the tenets of One Health. Programs to reduce ARG spread on dairy farms should focus on proper hand hygiene and PPE use at the level of knowledge, beliefs, and practices.Funding: Funding: was provided by the USDA-NIFA Food Safety Challenge (grant no. 2017-68003-26500).Disclosures: None

Measuring farmers' dynamic technical and udder health management inefficiencies: The case of Wisconsin dairy farms

This study measures the dynamic technical and udder health management inefficiencies of a sample of Wisconsin dairy farms. Udder health management inefficiency is defined as a farmer's failure to achieve lower levels of milk somatic cell counts compared with those of the best-practice farmers within the sample. The study proposes the treatment of somatic cell count as an undesirable output. We measured inefficiency using a dynamic directional distance function that accounts simultaneously for the expansion of desirable outputs and investments in capital assets, and contraction of undesirable output and variable inputs. In a second step, a bootstrap truncated regression was used to analyze factors that cause differences in dynamic technical and udder health management inefficiencies. Results showed that the sample farmers had considerably higher udder health management inefficiency scores than technical inefficiency scores. The results of the second-stage analysis indicated that technical inefficiency was influenced by summer precipitation and farmers' financial characteristics, and was regionally heterogeneous. Udder health management inefficiency was affected by summer temperature and nonfarm income. By ranking farms in this study in terms of technical and udder health management inefficiency, we allowed inefficient farms to compare their performance with that of their efficient peers, and thus identify targets for production and udder health management improvement efforts. Finally, although our study focused on farmers' performances with respect to udder health management, the proposed modeling framework can be applied to the management of other animal diseases and welfare conditions.

Life Cycle Assessment of Struvite Precipitation from Anaerobically Digested Dairy Manure: A Wisconsin Perspective.

Recovering valuable nutrients (e.g., P and N) from waste materials has been extensively investigated at the laboratory scale. Although it has been shown that struvite precipitation from several manure sources contributes to nutrient management practices by recovering valuable nutrients and preventing them from reaching water bodies, it has not been widely applied in commercial (i.e., farm) scales. The purpose of this study is to evaluate the potential environmental impacts of the struvite recovery process from the liquid portion of the anaerobically digested dairy cow manure generated in Wisconsin, USA, dairy farms using life cycle assessment methodology for both bench- and farm-scale scenarios. The struvite precipitation process involves the use of additional chemicals and energy; therefore, investigating upstream impacts is crucial to evaluate the environmental costs and benefits of this additional treatment process. Results indicate that up to a 78% impact decrease in eutrophication potential can be achieved when P and N are recovered in the form of struvite and are applied in lieu of conventional fertilizers, rather than using the liquid portion of the anaerobically digested dairy manure as a fertilizer. Additionally, significant differences are identified in the majority of environmental impact categories when the struvite precipitation process is modeled and evaluated in a farm-scale setting. Future work should expand to evaluate the overall environmental impacts and trade-offs of struvite recovery application, including the anaerobic digestion system itself at the farm scale. Integr Environ Assess Manag 2021;17:292-304. © 2020 SETAC.

Chronological set of E. coli O157:H7 bovine strains establishes a role for repeat sequences and mobile genetic elements in genome diversification

BackgroundEnterohemorrhagic Escherichia coli O157:H7 (EHEC) is a significant foodborne pathogen that resides asymptomatically within cattle and other ruminants. The EHEC genome harbors an extensive collection of mobile genetic elements (MGE), including multiple prophage, prophage-like elements, plasmids, and insertion sequence (IS) elements.ResultsA chronological collection of EHEC strains (FRIK804, FRIK1275, and FRIK1625) isolated from a Wisconsin dairy farm (farm X) comprised a closely related clade genetically differentiated by structural alterations to the chromosome. Comparison of the FRIK804 genome with a reference EHEC strain Sakai found a unique prophage like element (PLE, indel 1) and an inversion (1.15 Mb) situated symmetrically with respect to the terminus region. Detailed analysis determined the inversion was due to homologous recombination between repeat sequences in prophage. The three farm X strains were distinguished by the presence or absence of indel 3 (61 kbp) and indel 4 (48 kbp); FRIK804 contained both of these regions, FRIK1275 lacked indel 4, and indels 3 and 4 were both absent in FRIK1625. Indel 3 was the stx2 prophage and indel 4 involved a deletion between two adjacent prophage with shared repeat sequences. Both FRIK804 and FRIK1275 produced functional phage while FRIK1625 did not, which is consistent with indel 3. Due to their involvement in recombination events, direct and inverted repeat sequences were identified, and their locations mapped to the chromosome. FRIK804 had a greater number and overall length of repeat sequences than E. coli K12 strain MG1655. Repeat sequences were most commonly associated with MGE.ConclusionsThis research demonstrated that three EHEC strains from a Wisconsin dairy farm were closely related and distinguished by variability within prophage regions and other MGE. Chromosome alterations were associated with recombination events between repeat sequences. An inventory of direct and inverted repeat sequences found a greater abundance and total length of repeat sequences in the EHEC strains compared to E. coli strain MG1655. The locations of the repeat sequences were biased towards MGE. The findings from this study expand our understanding of the precise molecular events and elements that contributed to genetic diversification of wild-type EHEC in the bovine and farm environments.

Bovine Tuberculosis: A Re-emerging Zoonotic Infection

ABSTRACT Bovine tuberculosis is caused by Mycobacterium bovis (M. bovis), which infects both humans and cattle. In 2018, a dairy farm in Wisconsin was affected by M. bovis, including a farmworker with exposure to the affected herd. Largely eradicated by effective public health strategies in the United States, most cases are now associated with risk factors including occupational hazards, food consumption, and iatrogenic infections. M. bovis continues to cause disease worldwide affecting certain at-risk populations in the United States. Infections more often result in extrapulmonary sequelae and resistance to pyrazinamide is universal. Thus, successful treatment depends on early and correct identification of the mycobacterium species. A One Health approach to control this re-emerging disease is crucial.

Molecular epidemiology of nonsevere clinical mastitis caused by Klebsiella pneumoniae occurring in cows on 2 Wisconsin dairy farms

The objective of this study was to describe diversity of Klebsiella pneumoniae isolated from milk collected at detection of nonsevere (abnormal milk or abnormal udder) clinical mastitis (CM) and during a follow-up period. Cases were detected in cows enrolled in a randomized clinical trial (n = 168) conducted using 2 related Wisconsin dairy farms. Cases were randomly assigned to receive 2 d (n = 18) or 8 d (n = 18) of intramammary infusions with an approved product containing ceftiofur hydrochloride or assigned to a negative control group (n = 17). Milk samples were collected from affected quarters at detection and during a 28-d follow-up period. Sufficient DNA was recovered from 53 of 54 Kleb. pneumoniae cultured from quarter milk samples collected at detection of the incident case. Additional Kleb. pneumoniae were recovered from milk samples collected from the same quarters at 14, 21, and 28 d after case detection (n = 35), at detection of recurrent cases in the same quarter (n = 14), and from new cases of CM (n = 3) occurring in enrolled quarters. All Kleb. pneumoniae were used for molecular typing by pulsed-field gel electrophoresis and 90% similarity was used to define homology. Of Kleb. pneumoniae recovered from incident cases, unique strains (n = 41) were identified in milk samples collected from cows on farm A (n = 19) and farm B (n = 22), whereas 12 clonal strains were identified with 8 found only in milk collected from farm A and 4 found in milk samples collected from cows on both farms. Heterogeneous strains of Kleb. pneumoniae genotypes were isolated from incident cases of CM. However, when intramammary infection persisted or when recurrence of CM occurred, clonal strains were isolated at 14, 21, or 28 d. Similar strains of Kleb. pneumoniae genotypes caused persistent CM. In conclusion, initial cases of CM were caused by a wide genetic diversity of Kleb. pneumoniae, but when IMI persisted, the same strain often persisted within the mammary gland up to 28 d.

Improving nutritional accuracy and economics through a multiple ration-grouping strategy

The objective of this study was to develop a model application to systematize nutritional grouping (NG) management in commercial dairy farms. The model has 4 sub-sections: (1) real-time data stream integration, (2) calculation of nutritional parameters, (3) grouping algorithm, and (4) output reports. A simulation study on a commercial Wisconsin dairy farm was used to evaluate our NG model. On this dairy farm, lactating cows (n = 2,374 ± 185) are regrouped weekly in 14 pens according to their parity and lactation stage, for which 9 diets are provided. Diets are seldom reformulated and nutritional requirements are not factored to allocate cows to pens. The same 14 pens were used to simulate the implementation of NG using our model, closely following the current farm criteria but also including predicted nutritional requirements (net energy for lactation and metabolizable protein; NEL and MP) and milk yield in an attempt to generate more homogeneous groups of cows for improved diet accuracy. The goal of the simulation study was to implement a continuous weekly system for cows' pen allocation and diet formulation. The predicted MP and NEL requirements from the NG were used to formulate the diets using commercial diet formulation software and the same feed ingredients, feed prices, and other criteria as the current farm diets. Diet MP and NEL densities were adjusted to the nutritional group requirements. Results from the simulation study indicated that the NG model facilitates the implementation of an NG strategy and improves diet accuracy. The theoretical diet cost and predicted nitrogen supply with NG decreased for low-nutritional-requirement groups and increased for high-nutritional-requirement groups compared with current farm groups. The overall average N supply in diets for NG management was 15.14 g/cow per day less than the current farm grouping management. The average diet cost was $3,250/cow per year for current farm management and $3,219/cow per year for NG, which resulted in a theoretical $31/cow per year diet cost savings.

Corrigendum: Climatic effects and total factor productivity: econometric evidence for Wisconsin dairy farms

Corrigendum: Climatic effects and total factor productivity: econometric evidence for Wisconsin dairy farms

Climatic effects and total factor productivity: econometric evidence for Wisconsin dairy farms

AbstractThis study exploits temporal and cross-sectional variation in weather and long-run climate trends to investigate their effects on farm-level productivity. Using panel data for a sample of Wisconsin dairy producers, three stochastic production frontier models are estimated and a random parameters approach is chosen as the most desirable, which accounts for stochastic observed and unobserved environmental factors. The estimated coefficients are used to decompose a multiplicative total factor productivity index that accounts for different sources of productivity growth. Annual productivity growth is estimated at 2.16 per cent, driven primarily by technical progress (1.91 per cent per annum). The average per year contribution of the other productivity components is: climate adaptation efforts −0.31 per cent; scale-mix efficiency change +0.13 per cent and technical efficiency +0.05 per cent.

Genomic analysis of bovine respiratory disease and lung consolidation in preweaned Holstein calves using clinical scoring and lung ultrasound

Bovine respiratory disease (BRD) is a leading cause of morbidity and mortality in dairy calves, with detrimental long-term effects that include stunted growth, increased age at first calving, and decreased milk production in first lactation. The objectives of this study were to establish a protocol for objective and efficient assessment of BRD phenotypes in preweaned dairy calves, develop a genomic reference population with well-defined clinical and subclinical phenotypes, identify chromosomal regions associated with BRD in a genome-wide association study, estimate genetic parameters of BRD, and predict genomic breeding values of dairy calves. A total of 1,107 Holstein calves from 6 dairy farms in southern Wisconsin were examined using clinical respiratory scoring and lung ultrasound at 3 and 6 wk of age. The clinical respiratory score was based on visual appraisal of eyes, nose, ears, cough, and temperature. Lung ultrasound scores were assigned based on the amount of consolidation present. Calves were genotyped with a commercially available SNP array and after quality control and imputation to higher density, 690,291 SNP markers and 1,014 individuals remained. Single-step genome-wide association study and single-step genomic best linear unbiased prediction were applied to binary 3- and 6-wk phenotypes considered as overall respiratory healthy versus affected (RESP3, RESP6) or as presence or absence of lung consolidation (CON3, CON6). Lung ultrasound combined with a clinical scoring system allowed for efficient and objective assessment for the prevalence of BRD. Proportions of variance attributed to 1-Mb non-overlapping windows suggested genomic regions that may contain putative candidate genes, most notably regions on Bos taurus autosomes 1, 6, 7, 10, 11, 12, 15, 17, 18, 27, and 28 that explained 0.70 to 1.45% of the genetic variance. Heritability estimates were higher at 3 wk (0.214 and 0.241 for CON3 and RESP3, respectively) than 6 wk (0.084 and 0.111 for CON6 and RESP6, respectively), and mean reliabilities of genomic estimated breeding vales for calves with genotypes and phenotypes ranged from 0.12 for CON6 to 0.30 for RESP3.

Short communication: Longitudinal study of quarter-level somatic cell responses after naturally occurring, nonsevere clinical mastitis diagnosed as culture negative, or caused by Escherichia coli or Klebsiella pneumoniae, and randomly assigned to a no-treatment group or to receive intramammary ceftiofur

The objective of this study was to describe weekly quarter-level somatic cell count (QSCC) after occurrence of nonsevere clinical mastitis (CM) that was diagnosed as culture negative, or caused by Escherichia coli or Klebsiella pneumoniae. All cases occurred in cows enrolled in negatively, controlled randomized clinical trials. We hypothesized that after occurrence of CM, QSCC patterns would vary among etiologies and this effect would not be mitigated by treatment using intramammary (IMM) ceftiofur. Data from two previously published randomized clinical trials performed on 3 Wisconsin dairy farms were used. Only cases confirmed as culture negative (NG) or E. coli or Kleb. pneumoniae (GRAMNEG) were used for analysis. In NG, cows were assigned to no antimicrobial treatment (negative control, n = 44) or 5 d of once daily IMM (n = 41) infusions with an approved product containing ceftiofur hydrochloride. In GRAMNEG, cows were assigned to IMM treatment with the same ceftiofur product for 2 different durations (2 d, n = 36; or 8 d, n = 38) or no antimicrobial treatment (negative control, n = 36). For quarters enrolled in NG, no significant differences were identified for weekly QSCC between quarters in the treated or negative control groups (5.4 log10SCC for both groups). For quarters enrolled in GRAMNEG, no significant differences were identified for QSCC between quarters that received the 2-d (6.2 log10SCC) or 8-d (6.3 log10SCC) IMM treatment or were in the negative control group (6.0 log10SCC). At the pathogen level, regardless of treatment, QSCC varied among pathogens and log10SCC were 5.4 (culture negative), 5.8 (E. coli), and 6.2 (Kleb. pneumoniae). Patterns of QSCC of CM diagnosed as culture negative and E. coli were similar in magnitude and time to resolution of the inflammatory response. In conclusion, as compared to CM diagnosed as culture negative or caused by E. coli, CM caused by Kleb. pneumoniae was associated with poorer outcomes. Regardless of IMM ceftiofur treatment, the immune response of the cow resulted in rapid reduction of SCC of quarters diagnosed as culture negative and quarters with CM caused by E. coli. In contrast, the SCC remained elevated for quarters with CM caused by Kleb. pneumoniae and a greater proportion of those cases remained chronically infected.

Analysis of beneficial management practices to mitigate environmental impacts in dairy production systems around the Great Lakes

The influence of farm-specific beneficial management practices (BMPs) on a set of comprehensive environmental impacts was characterized and quantified for two representative dairy farms in the Great Lakes region (a large 1500-cow farm in New York (NY) and a smaller 150-cow farm in Wisconsin (WI)). Comparative benefits or drawbacks of the effect of the adoption of selected management scenarios to environmental impacts were estimated by coupling the output from the Integrated Farm System Model (IFSM) to provide lifecycle inventory data for SimaPro©. The small dairy farm in WI generated a consistently larger carbon footprint than the large dairy farm in NY due to greater enteric methane (CH4) emissions from different feeds fed and greater nitrous oxide (N2O) emissions from the bedded pack housing facility for young stock. The flare scenario of burning biogas produced in a covered manure storage on the small farm and a whole-farm mitigation plan of combined feed, field, and manure management in the large farm demonstrate significant potential to reduce overall carbon footprint by 20.0% and 25.8%, respectively, compared to the baselines. The assessments of selective impact categories such as fossil energy use, water use, land occupation, aquatic eutrophication, terrestrial acidification, respiratory effects, human toxicity, and ecotoxicity are discussed and highlight hot spots relevant for sustainable dairy farm management. Normalization analysis indicates that eutrophication potential is the largest relative impact profile, which suggests that efforts to mitigate eutrophication can achieve relatively greater environmental impact reduction. Although this study identifies the beneficial adaptation of sustainable dairy production practices on individual impact profiles, trade-offs between impact categories make the analysis more complex when considering the comprehensive suite of environmental impacts.

Physical Properties of Dairy Manure Pre- and Post-Anaerobic Digestion

Manure characteristics change through processing, including anaerobic digestion (AD). These changes can alter handling of manure during downstream operations. This study analyzed the density, total solids (TS) content, and volatile solids (VS) content of pre-digested and anaerobically digested dairy manure from seven dairy farms in Wisconsin. The density of pre-digested manure increased from 990 to 1065 kg m−3 as the TS level increased from 1.5% to 13.0%. Density and TS for pre-digested manure from facilities using separated solids as bedding were related with a linear model for TS ranging from 1.5% to 13.0% and with a polynomial model for TS ranging from 1.5% to 50%. The model shows that density decreases with an increasing TS content when TS is greater than 8.0%. Manure from dairy facilities that used sand bedding had a VS/TS ratio of 0.87. This ratio was higher than the ratio when manure solids were used as bedding (0.81) and when food waste was incorporated into the digester (0.77). This study also provides a simple methodology to estimate biogas yield by using the density of pre- and post-digested manure.

An income over feed cost nutritional grouping strategy

This study introduces a new nutritional grouping method, OptiGroup, which maximizes milk income over feed cost (IOFC) using a mixed-integer nonlinear programming optimization algorithm. Analyses compared the OptiGroup with the cluster method, the current state-of-the-art nutritional grouping technique. Analyses were performed using cow-level data from 7 Wisconsin dairy farms. Consistently, the OptiGroup and the cluster were constrained to group cows simultaneously into 2 (low and high nutrient requirements) and 3 (low, medium, and high nutrient requirements) same-size groups. Each diet satisfied the net energy (NEL) and crude protein (CP) requirements of approximately 83% of the cows in each group by using lead factors based on nutrient density. A control treatment (1-group scenario) was used as a baseline for comparisons. The IOFC, dietary nutrient densities (NEL and CP), and dry matter intake with both methods were computed and compared. The percentage of cows grouped differently and the percentages of primiparous cows and late-lactation (>200 d in milk) cows in each group were also analyzed. Results were as follows: (1) average extra IOFC of $8/cow per yr (2-group) and $12/cow per yr (3-group) by switching from cluster to OptiGroup method; (2) difference between dietary nutrient densities of the groups were reduced under OptiGroup method compared with cluster (i.e., NEL differences in 2 groups were 0.20 Mcal/kg for the cluster vs. 0.11 Mcal/kg for OptiGroup); (3) dry matter intake decreased with increasing group numbers within a grouping method, and decreased from cluster to OptiGroup method with constant group numbers; (4) percentage of primiparous cows was greater in the low group of cluster and in the high group of OptiGroup; and (5) proportion of late-lactation cows tended to be greater in the low group in both grouping strategies. Results indicated that the OptiGroup performed economically better than the cluster because of nutrient savings, even with high feed cost conditions. This study offers a new nutritional grouping paradigm, which could improve herd management on dairy farms. However, animal trials are needed to validate this new nutritional grouping method under farm conditions.

Impact of Dairy Manure Processing Using Polyacrylamide on Antibiotic-Resistant Bacterial Level

This study investigates levels of bacteria through population indicators as well as the levels of antibiotic-resistance bacteria in dairy manure. Although overall bacteria levels may be reduced during manure processing, it is of interest whether changes in management practices could lead to increased levels of antibiotic-resistance bacteria, which are becoming more prevalent in agricultural soils, groundwater, and surface water. Appropriate manure treatments are needed not only to reduce the potential risk of exporting antibiotic-resistant bacteria to an environment, but also reduce antibiotic-resistant bacteria exposure to animals if processed water is recycled. Results from this research revealed manure separation under relatively low speed centrifuge with 100 ppm polyacrylamide (PAM) emulsion addition reduced bacteria indicators population such as total coliforms and Escherichia coli (E. coli) significantly in the liquid stream compared to no PAM added. However, the percentages of antibiotic-resistant isolates in liquid stream after centrifuge with PAM were higher compared to raw manure and no PAM added. Antibiotic resistance (cephalosporin, florfenicol, penicillin, or tetracycline) was observed or 65.38% of bacterial isolates in manure from a large dairy farm in Wisconsin and 39.29% of isolates demonstrated multidrug resistance. The results from this study strongly suggest that appropriate manure treatment is essential in order to help minimize the abundance of antibiotic resistance in our water environment.

Life-cycle Analysis of Advanced Manure Management Systems for a Wisconsin Confined Animal Feeding Operation (CAFO)

Abstract. Manure management is becoming an increasing burden for large dairy farms in Wisconsin and across the United States due to land application limitations for nutrients and hauling costs. One solution is to install advanced manure management systems, which remove large portions of water from the manure and treat it to a level sufficient for surface water discharge, livestock consumption, or irrigation. These systems can be divided into a pretreatment stage, which consists of solids removal, and a treatment stage, which generally consists of membranes. Not only does this approach reduce the quantity of material that is land applied, it can separate the nutrients into individual manure streams that can be more easily managed. However, there is still limited understanding regarding the costs and operation and maintenance requirements of these systems. To this end, a life-cycle analysis was completed for five commercially available advanced manure management systems based on how they would treat manure from a 3,500-cow dairy farm in Wisconsin, which was concerned only with the drawbacks of hauling and not application limitations. The evaluation included both an economic and non-economic analysis. The economic analysis consisted of a 20-year total present worth life-cycle cost analysis. The non-economic analysis consisted of a comparison of a set of non-economic factors that matched the management goals and strategies identified by the participating farm. Overall, all five systems evaluated addressed nutrient limitations and hauling costs. No treatment system had a payback less than 20 years at a hauling cost of $0.0040 L-1 ($0.015 gal-1). However, two systems had a payback period less than 20 years for a hauling cost of $0.0053 L-1 ($0.02 gal-1). Furthermore, if capital costs were ignored, the cost of recovered water was comparable to hauling costs for four of the alternatives. Still, there was high uncertainty in the estimated costs due to the low number of current installations of these systems on CAFOs. There was also a wide range of responses to the non-economic evaluation metrics, especially regarding performance, nutrient management techniques, chemical usage, and estimated regular operation and maintenance, which was again, likely due to the limited number of farm installations. Future work will verify actual costs and performance of these systems once implemented. Keywords: Advanced Manure Management, CAFOs, Dairy, Life-cycle analysis.

An overview and impact assessment of OSHA large dairy local emphasis programs in New York and Wisconsin.

Farming has been exempted from most labor regulations and shielded from regulatory scrutiny by the Occupational Safety and Health Administration (OSHA). Yet, agriculture and dairy in particular, has relatively high injury and fatality rates. A recent shift in OSHA's approach to agricultural worker safety and health includes two dairy-focused Local Emphasis Programs (LEPs), one launched in Wisconsin in 2011 and the other in New York in 2014. We examine data from LEP-related, OSHA consultations and inspections as well as non-governmental audit programs, and review farmer perceptions about the LEP. Inspections conducted by OSHA and private consultation programs highlight the presence and variety of hazards on dairy farms in Wisconsin and New York. The LEPs helped raise dairy producers' awareness of inherent hazards and methods to correct them. Farmers cited the LEP as beneficial, identifying it as a catalyst to reduce hazards on their farms.

Repro Money: An Extension Program to Improve Dairy Farm Reproductive Performance

A farmer-directed, team-based Extension program (Repro Money) was developed and executed by the University of Wisconsin–Madison Department of Dairy Science in collaboration with University of Wisconsin–Extension. The goal of the Repro Money program was to help Wisconsin dairy farmers increase reproductive performance and profitability through identification of areas for improvement and implementation of action plans. For the 40 Wisconsin dairy farms that completed the Repro Money program, mean 21-day pregnancy rate increased by 2 percentage points, which was estimated to result in an economic net gain of $31 per cow per year. Extension professionals can apply similar team-based programs to tackle multifaceted, interrelated problems that may be only partially addressed by other, more traditional programming.

Management, operational, animal health, and economic characteristics of large dairy herds in 4 states in the Upper Midwest of the United States

Recent trends in dairy farm structure in the United States have included a decreasing number of farms, although farm size has increased, especially the share of milk production from very large herds (>2,500 cows). The objectives of this observational study were to describe common management practices; to characterize labor and operational structure; to measure some aspects of animal health, including lameness, hock lesions, mortality, and mastitis incidence; and to summarize cost of production on farms with more than 2,500 cows in 4 states in the Upper Midwest of the United States. The study included 15 dairy farms in Minnesota, Wisconsin, Iowa, and South Dakota. Farms were visited twice, once each year, and on-farm herd records were collected for those 2 yr. On-farm herd records were used to investigate mortality, culling, pregnancy rate, and clinical mastitis incidence. At least 1 high-producing pen of mature cows and 1 pen of fresh cows were scored for locomotion. Likewise, at least 1 pen of high-producing mature cows was scored for cleanliness and hock lesions. Median herd size was 3,975 cows (range = 2,606-13,266). Milk sold per employee was 1,120,745 kg and the number of cows per employee was 105. Eighty percent of the farms had Holstein cows, 13% had Jersey, and 7% had Jersey-Holstein crosses. All farms used artificial insemination as the sole form of breeding and 100% of the farms used hormonal synchronization or timed artificial insemination programs in their reproductive protocols; 21-d pregnancy rate was 21.7%. Median lameness prevalence was 18.3% and median severe lameness prevalence was 5.1%. Median hock lesion prevalence was 17.4% and median severe hock lesion prevalence was 1.9%; mortality rate was 7.4%. Clinical mastitis incidence was 62.5 cases per 100 cow-years. Feed costs accounted for approximately 53% of the total cost of producing milk, followed by labor at 11%, interest and depreciation expenses at 10%, and replacement costs at 9.5%. Herds in the top 50th percentile for profitability had a net income of $2.40 per hundredweight of milk sold compared with $0.95 per hundredweight for herds in the bottom 50th percentile. Although results of this study were helpful in understanding how large dairy systems operate in the Upper Midwest, more research is yet needed with a larger number of farms and wider variety of management practices to identify factors within these large farms that promote optimal animal health and profitability.