Food security has always been one of the most important goals for government at all levels in China. Although more fertilization is an effective solution, many other problems appeared with the overuse of fertilization, such as water pollution, soil compaction and so on. Thus, in order to provide suitable advice on fertilization, we used the combination of high-resolution thermal and visible images to evaluate the transpiration rate and canopy cover and identify the effects of fertilization on crop growth. Three different fertilization levels of: (A) 900 kg/ha, (B) 675 kg/ha, and (C) 450 kg/ha were designed for winter wheat. Spatial-temporal distribution of transpiration rate and canopy coverage under different growth stages were depicted based on three temperature model and canopy software. Results showed that: (1) the daily variation of canopy temperature (Tc) and transpiration rate were both unimodal curve, and both of them reached their daily peak values at 14:00. Moreover, the spatial heterogeneity of land surface temperature (LST) and transpiration rate was quite obvious and common for all growth stages, and this spatial heterogeneity presented variation from pixel to pixel. (2) Fertilization had a significant effect on the growth of winter wheat. With the higher fertilization amount, the growth of winter wheat became more vigorous, the canopy cover and transpiration rate turned higher, and the Tc was lower. For example during recovering stage, the canopy cover under treatment A、B and C were 78.7%, 52.5%, and 42.7%, respectively, as well as the peak values of transpiration rate for treatment A, B, and C were 0.45 mm/h, 0.29 mm/h, and 0.12 mm/h, respectively. The transpiration rate had a positive correlation with canopy cover, and the coefficients of determination R2 = 0.78. (3) The advancement in the crop growth led to the increase of canopy cover and transpiration rate for winter wheat under different fertilization levels. However, the difference of transpiration rate and canopy cover under different fertilization levels decreased gradually with crop growth. Our research accurately detected the effects of fertilization on crop growth based on high-resolution thermal and visible images, which will provide feasible assistance for rational fertilization and it is possible to foresee the broad application prospects in the future.