Daily Milk Intake Research Articles

Tailored recommendations for infant milk formula intake results in more accurate feeding.

Based on theoretical calculations and actual formula intake data, we conclude that larger infants require a significantly higher daily formula milk intake than smaller infants, and we postulate that infants could benefit from more tailored formula milk intake recommendations. • Adequate energy intake during the infancy period is crucial to support optimal growth and organ development, with the potential for long-lasting health effects. • Current available guidelines on the daily formula milk requirements of infants are based on the needs of infants with their growth pattern following the 50th percentile of the weight-for-age growth curve. • Based on using both theoretical calculations and actual formula intake data, larger infants require a significantly higher daily formula milk intake than smaller infants. • Exclusive formula-fed infants could benefit from more tailored formula milk intake recommendations, in early infancy.

74 Feeding style and its influence on growth, circulating insulin, and spatial preference of young pigs

Abstract In this study, we investigated the influence of feeding style on growth performance, circulating insulin concentrations, and spatial preference within the home-cages (HC) of young pigs. Pigs (n = 43 boars) were weaned on postnatal day (PND) 2, allotted to treatment based on litter of origin and body weight (BW), and reared artificially over a 15-d period. Pigs were provided a nutritionally adequate milk replacer 20 h per day using one of two feeding styles: 1) ad libitum (i.e., continual access to milk replacer), and 2) prescribed [330 mL/kg of BW from PND 3-7 (wk 1), and 357.5 mL/kg of BW from PND 8-18 (wk 2)]. In addition to water in the reconstituted milk replacer, free choice access to fresh water was provided throughout the study. Milk intake (i.e., disappearance) and BW were recorded daily, with pigs undergoing continuous top-down video surveillance throughout the study. On PND 17, blood was collected from a subset of pigs (n = 24) for quantification of insulin concentrations. Video analysis of pigs (n = 43) was performed to establish time spent in each HC quadrant. All data were analyzed via a 1-way ANOVA using the MIXED procedure of SAS. During wk 1, ad libitum pigs exhibited increased (P < 0.05) average daily BW gain (152 g/d) and average daily milk intake (728 g/d) compared with the prescribed treatment (127 g/d and 570 g/d, respectively). This same effect continued in wk 2 for average daily BW gain and average daily milk intake, with ad libitum-fed pigs consuming more (P < 0.05) milk replacer (256 g/d and 1,356 g/d, respectively) compared with prescribed-fed pigs (192 g/d and 974 g/d, respectively). Over the entire 15-d feeding study, average daily BW gain (234 g/d), average daily feed intake (1,140 g/d) and milk intake-to-BW ratio (4.60) were greater (P < 0.05) in the ad libitum treatment compared with prescribed feeding (180 g/d, 830 g/d, and 3.81 respectively). Insulin concentrations were greater (P < 0.05) in the ad libitum treatment (348 µIU/mL) 30 min after feed provision compared with the prescribed treatment (190 µIU/mL). Video analysis of spatial preference within HC suggested pigs in the prescribed treatment spent more (P < 0.05) time in quadrant 4 during wk 1 (5.43% of time recorded) compared with the ad libitum treatment (3.82% of time recorded), likely due to quadrant 4 housing the feed bowl. However, all other time points had no effect on spatial preference. Overall, the type of feeding style altered growth performance and insulin concentrations within young pigs and may impact the spatial preference of pigs within their home-cage environment.

Evaluating the dietary quality of 454 rural elderly aged 65 years and above in Luzhou City in 2023

To evaluate the dietary quality of the rural elderly aged 65 years and above. In February-March 2023, a convenience sampling method was adopted to select 454 rural elderly aged 65 years and above in a township of Luzhou City. The dietary survey was conducted using a semi-quantitative food frequency questionnaire(FFQ-25), and the questionnaire information was collected by face-to-face interviews. Dietary quality was evaluated using the Dietary Balance Index-16(DBI-16) score. The proportion of older people in the region with moderate and high dietary imbalances was 79.7%. Inadequate and excessive dietary intake coexisted. The average daily intake of cereals and potatoes and livestock and meat foods were 356.7 g and 76.2 g, exceeding the recommended intake. The average daily intake of fruit, milk and fish and shrimp intake was 22.8 g, 36 g and 3.7 g, respectively, which was only 10% of the recommended amount, and the intake was seriously insufficient. In addition, the degree of food diversity is relatively low, with most of the average daily intake of food types ranging from five to eight, and only 4.6% of the elderly having more than eight. A total of seven dietary patterns were found among the rural elderly in the region, including a certain degree of under-consumption pattern, a severe under-consumption pattern, a certain degree of over-consumption pattern, and a pattern of both under-consumption and over-consumption. That was dominated by the pattern of severe underconsumption and the pattern of some degree of underconsumption and higher degree of overconsumption, which accounted for 72.3% of the total. The rural elderly aged 65 years and above in Luzhou City have a serious dietary imbalance, with a high proportion of insufficient intake of vegetables, fruits and milk, as well as aquatic products and eggs; and excessive intake of livestock, poultry, meat and cereals and potatoes.

Influence of Maternal Dietary Pattern on the Pro-Oxidant and Antioxidant Status of Mother's Own Milk.

Human milk is essential for a child's best development. However, what a mother eats while breastfeeding can directly influence the composition of mother's milk. This study aimed to assess the antioxidant-oxidant profile of human milk and establish a connection between this profile and the dietary habits of the mothers. A cross-sectional study was conducted at the Hospital Infantil e Maternidade Alzir Bernardino Alves (HIMABA), located in the municipality of Vila Velha-ES, Brazil. The sample included 98 participants. All volunteers completed a structured interview and a Food Frequency Questionnaire. Approximately 5-10 ml of colostrum, transitional milk, and mature milk were manually collected. The antioxidant activity of human milk was assessed using the colorimetric method for free radical scavenging with 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid. Oxidative stress was determined by measuring lipid peroxidation through malondialdehyde concentration, evaluating advanced oxidation protein products, and assessing total protein content using the Bradford method. The antioxidant profile of colostrum was higher than that observed in later phases of milk, whereas pro-oxidants increased in later phases. Maternal dietary patterns influenced the pro-oxidant status of human milk. Participants with a higher daily intake of milk, dairy products, vegetable oils, olive oils, and legumes exhibited lower levels of lipid peroxidation in colostrum, transition milk, and mature milk, respectively. Our study highlights the vital role of a balanced maternal diet in shaping the pro-oxidant status of human milk, with implications for infant health.

The effect of in-hospital breast milk intake on the gut microbiota of preterm infants

The aim of this study was to explore the effect of in-hospital breast milk intake on the development of early gut microbiota in preterm infants in two dimensions: longitudinal over time and cross-sectional between groups. Researchers collected preterm infants' general data baseline characteristics, recorded their daily breast milk intake, probiotics, and antibiotics use, and collected their stool specimens at 1st week, 2ndweek, 3rd week and 4th week after birth. The researchers analyzed the effect of breast milk on gut microbiota of preterm infants by bioinformatics methods of intra-group longitudinal variation of gut microbiota structure and diversity in preterm infants and cross-sectional differences between >70% in-hospital breast milk intake (BM) group and ≤70% (PF) group. A total of 60 preterm infants were included in this study, and a total of 213 stool specimens were retained. BM had statistically different Shannon and Simpson indices between the first and fourth week after admission (P<0.05), both of them showed a lower diversity in the later week than in the previous week. The Shannon index and Simpson index of BM from week 3 onwards were statistically different from PF (P<0.05), and the Shannon index and Simpson index of BM were lower than those of PF. Significantly statistical differences (P<0.05) were found in the beta diversity of gut microbiota in preterm infants as time progressed, and both showed a lower beta diversity in the later week than in the preceding week. The dominant taxa of PF in the first postnatal week were Bifidobacterium animalis, etc., the dominant taxa of BM in the third postnatal week were Clostridium_sensu_stricto _1, etc. CONCLUSIONS: The development and evolution of gut microbiota in preterm infants' in-hospital period was a continuous, non-random process, and similar trends in species composition and changes in gut microbes emerged in preterm infants with different ratio of breast milk intake. In the NICU setting, alpha diversity was lower in preterm infants in the >70% breast milk intake group than in the ≤70% group when compared between groups at the same time, which may be related to delayed maturation of gut microbes and represents a more developmental gut time window.

Effect of β-casein A1 or A2 milk on body composition, milk intake, and growth in Holstein, Simmental, and crossbred dairy calves of both sexes

The aim of this study was to compare the effects of feeding homozygous β-CN A1 or A2 milk on the body composition, milk intake, and growth of German Holstein (GH), German Simmental (GS), and crossbred (CR) dairy calves of both sexes during the first 2 wk of life. A total of 104 calves (n = 54 female, f; and n = 50 male, m) from the breed groups GH (n = 23), GS (n = 61), and crossbred GH × GS (n = 20) were evaluated. Calves were weighed after birth and received colostrum ad libitum. On the second day, calves were alternately housed in pairs in double-igloo systems according to their random birth order and received either A1 milk (n = 52; 27 female and 25 male) or A2 milk (n = 52; 27 female and 25 male). They were offered 7.5 L/d, and the individual actual total milk intake was recorded. Daily energy-corrected milk intake was also calculated based on the milk composition (fat and protein). Fecal scores were recorded daily. On d 15, visceral adipose tissue (VAT) volume was assessed by open magnetic resonance imaging and dual-energy X-ray absorptiometry (DXA). In addition, fat and lean mass (g), as well as bone mineral content (g) and bone mineral density (g/cm2), were determined by DXA. The body composition, milk intake, and growth were similar between the 2 types of milk in the first 2 wk of life. Female calves had more VAT and fat mass, but less lean mass than male calves. GH and CR calves had more VAT and less lean mass than GS calves. Male calves were heavier than female calves after birth and on d 15. The average days with diarrhea and diarrhea occurrence were similar between calves fed A1 and A2 milk and between both sex groups. GS calves presented slightly more days with diarrhea and increased odds of having diarrhea compared with GH calves, not differing from CR.

Adaptation of buffalo calves to a new automatic milk feeder

Rational heifer management in buffalo rearing is of paramount importance. There are no studies in the literature dealing with automatic feeding in individual boxes for buffalo calves. This study aims to deepen knowledge in this area and to evaluate the ability of buffalo calves to adapt to a mobile automatic feeder through data provided by the system's monitoring software. Data were collected on 3 different groups of 15 buffalo calves in succession with each other. The data on the daily feeding quantity and volume showed that 95.67 % of the calves were fed within the first day of stall in the automatic feeder. During the first 72 h in the calf house, the calves were fed from 2.49 ± 1.21 meals per day with an average feeding rate of 0.24 ± 0.06 L/min. The average daily milk intake was 3.57 ± 0.26 L/d, while the average milk intake during the first 72 h was 10.7 ± 0.77 L. The buffalo calves, therefore, showed themselves to be able to adapt to this type of feeding, making use of its advantages.

Milk fat globule membrane promotes brain development in piglets by enhancing the connection of white matter fiber trace

IntroductionBrain development during infancy is crucial for later health and development. Although Milk Fat Globule Membrane (MFGM) has been demonstrated to enhance brain development, further investigation is needed to determine the optimal dose.MethodsIn this study, 80 piglets aged 2 days were randomly assigned to four groups: Control group, MFGM-L (1.74 g MFGM per 100 g diet), MFGM-M (4.64 g MFGM per 100 g diet), and MFGM-H (6.09 g MFGM per 100 g diet). Daily body weight and milk intake of the piglets were recorded until 31 days postnatal. Learning and memory abilities were evaluated using the spatial T-maze test on day 15. MRI analysis was conducted to assess functional and structural changes in brain tissues. Additionally, mRNA and protein expression of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NTF-3) in the hippocampus and prefrontal cortex were evaluated.ResultsThe results indicated that the MFGM supplemented diet significantly improved the accuracy of the piglets in the T-maze test, with the MFGM-L group exhibiting the best performance. MRI showed no volumetric differences in the gray and white matter between the groups. However, the fractional anisotropy in the left and right hippocampus of piglets in the MFGM-L group was significantly higher than in the other three groups. Furthermore, there was a strong correlation between the accuracy of the T-maze test and hippocampal fractional anisotropy.DiscussionThe MFGM supplemented diet also increased the expression of BDNF in the cerebral cortex. However, the changes in BDNF were not consistent with the results of the T-maze test. In conclusion, adding 1.74 g MFGM per 100 g diet can significantly improve neonatal piglets’ learning and memory abilities, potentially by enhancing the connection of white matter fiber bundles in the brain.

52 Feeding Style and its Effect on Growth and Spatial Preference of Young Pigs

Abstract In this study we investigated the effects of feeding method on growth performance and spatial preference within the home-cages (HC) of young pigs. Pigs (n = 41 boars) were weaned at postnatal day (PND) 2, allotted to treatment controlling for litter and body weight (BW), and reared artificially over a 15-d period. Pigs were provided a nutritionally adequate milk replacer 20-h per day, dispensed in one of two ways: 1) ad libitum (continual access to milk replacer), and 2) prescribed [330 mL/kg of BW from PND 3-7 (week 1), and 357.5 mL/kg of BW from PND 8-18 (week 2)]. In addition to water in the reconstituted milk replacer, free choice access to water was provided throughout the study. Milk intake (i.e., disappearance) and BW were recorded daily, with pigs undergoing continuous video surveillance throughout the study. Video analysis of pigs (n = 6) was performed to establish time spent in each HC quadrant (Table 1). All data were analyzed via a 2-way ANOVA (feeding style vs. home-cage quadrant) using the MIXED procedure of SAS. During week 1, pigs in the ad libitum treatment had increased (P &amp;lt; 0.05) average daily gain (219 g/d) and average daily milk intake (1,014 g/d) compared with the prescribed treatment (183 g/d and 794 g/d, respectively). This same effect continued in week 2 for average daily milk intake, with ad libitum-fed pigs consuming more (P &amp;lt; 0.05) milk replacer (780 g/d) compared with prescribed-fed pigs (614 g/d). Ad libitum-fed pigs exhibited better (P &amp;lt; 0.05) feed efficiency [i.e., reduced G:F ratio (0.22 and 0.19, respectively)] compared with prescribed-fed pigs (0.24 and 0.21, respectively) during both weeks 1 and 2. Over the entire 15-d feeding study, average daily gain (253 g/d) and milk intake-to-BW ratio (0.59) were greater (P &amp;lt; 0.05) in the ad libitum treatment compared with prescribed feeding (206 g/d and 0.52, respectively). Video analysis of spatial preference within HC suggested pigs spent more (P = 0.08) time in quadrants 2 and 4 (33.6% and 35.5% of time recorded, respectively), likely due to proximity to the feeding bowl, compared with quadrant 3 (8.7% of time recorded) but this effect did not differ (P = 0.50) by feeding method. Overall, type of feeding style altered growth performance within young pigs and may impact the spatial preference of pigs within their home-cage environment.

RETRACTED ARTICLE: Association of milk consumption with the incidence of cholelithiasis disease in the US adult population

BackgroundCholelithiasis is a common digestive system disease that imposes major burden on patients and society. Investigating the relationship between dietary factors and cholelithiasis risk can provide a basis for disease prevention. Previous studies on milk intake and cholelithiasis incidence have been limited.Therefore, the aim of our study was to assess the association between milk consumption and the incidence of cholelithiasis in males and females.MethodsWe selected 14,722 adults (≥ 18 years old) from National Center for Health Statistics (NHANSE) 2017–2020, and collected general characteristics of patients in the database, such as age, gender, race and body mass index (BMI), as well as dietary information (milk consumption). The occurrence of cholelithiasis was used as the outcome event, and the group was divided into cholelithiasis and non-cholelithiasis groups according to the outcome event. We used logistic regression models in generalized linear model (GLM) functions, controlling for demographic, lifestyle, and dietary factors, to estimate the association between milk intake and the incidence of cholelithiasis in males and females.ResultsA total of 14,722 adults were included. In the present study, the overall weighted prevalence of cholelithiasis was 10.96%, with 15.18% and 6.48% prevalence in females and males, respectively. Compared to infrequent milk intake, frequent milk intake (once a week or more) in females was associated with reduced cholelithiasis risk (OR 0.74, 95% CI 0.61–0.90). Daily milk intake in males was also related to lower cholelithiasis risk (OR 0.69–0.82). As adjusted variables increased in the models, predictive performance was improved (AUC 0.711 in females, 0.730 in males).ConclusionsAppropriate milk intake may correlate with decreased cholelithiasis risk. Our study provides a basis for dietary interventions against gallstones, but prospective studies are needed to verify the results.

Evaluation of the frequency of consumption of various groups of dairy products by students of the Peoples’ Friendship University of Russia

BACKGROUND: Milk and dairy products are an important component of a balanced diet. Milk and dairy products deficiency contributes to the violation of the main health indicators of various age groups of the population.&#x0D; AIM: The purpose of this study was to analyze the frequency of consumption of various groups of dairy products by students.&#x0D; MATERIALS AND METHODS: 388 full-time students of the Medical Institute of the Peoples Friendship University of Russia took part in the cross-sectional study. Of all the participants, 185 were men and 203 were women; the average age was (20.42.2) and (21.91.7) years, respectively. Anthropometric measurements were performed according to the standard program. The frequency of consumption of various groups of dairy products was studied using a modified questionnaire on the frequency of consumption of products.&#x0D; RESULTS: analysis of the questionnaire on the frequency of consumption of products demonstrated that approximately 14% of the respondents did not consume milk over the past month, about a third cottage cheese, 21% natural sour-milk products and 16% sour-milk products with various fillers. Hard cheeses and pickled cheeses are absent from daily food rations of 7% and 20% of respondents, respectively. Daily consumption was recorded as follows: milk 24% of respondents; cottage cheese 5%; fermented milk products (natural and with flavored) 9% and 8%, respectively; hard cheese 18%. The study also revealed gender differences in the characteristics of consumer preferences: women are less likely to consume cream, sour cream, soft cheese and milk. Among female students, the prevalence of overweight individuals was higher and amounted to 25% (n=50), while among male students 19% (n=35). A modest inverse relationship was found between daily milk intake and body weight, as well as between milk intake and body mass index (r=0.61 and r=0.65, respectively).&#x0D; CONCLUSIONS: our study revealed insufficient consumption of milk and dairy products among students. The relationship between the frequency of their consumption and indicators of physical development was noted.

Validation of a batch cultivation protocol for fecal microbiota of Kenyan infants

BackgroundThe combination of cultivation studies with molecular analysis approaches allows characterization of the complex human gut microbiota in depth. In vitro cultivation studies of infants living in rural sub-Saharan Africa are scarce. In this study, a batch cultivation protocol for Kenyan infant fecal microbiota was validated.MethodsFresh fecal samples were collected from 10 infants living in a rural area of Kenya. Samples were transported under protective conditions and subsequently prepared for inoculation within less than 30 h for batch cultivation. A diet-adapted cultivation medium was used that mimicked the daily intake of human milk and maize porridge in Kenyan infants during weaning. 16 S rRNA gene amplicon sequencing and HPLC analyses were performed to assess the composition and metabolic activity, respectively, of the fecal microbiota after 24 h of batch cultivation.ResultsHigh abundance of Bifidobacterium (53.4 ± 11.1%) and high proportions of acetate (56 ± 11% of total metabolites) and lactate (24 ± 22% of total metabolites) were detected in the Kenyan infant fecal microbiota. After cultivation started at an initial pH 7.6, the fraction of top bacterial genera (≥ 1% abundant) shared between fermentation and fecal samples was high at 97 ± 5%. However, Escherichia-Shigella, Clostridium sensu stricto 1, Bacteroides and Enterococcus were enriched concomitant with decreased Bifidobacterium abundance. Decreasing the initial pH to 6.9 lead to higher abundance of Bifidobacterium after incubation and increased the compositional similarity of fermentation and fecal samples. Despite similar total metabolite production of all fecal microbiota after cultivation, inter-individual differences in metabolite profiles were apparent.ConclusionsProtected transport and batch cultivation in host and diet adapted conditions allowed regrowth of the top abundant genera and reproduction of the metabolic activity of fresh Kenyan infant fecal microbiota. The validated batch cultivation protocol can be used to study the composition and functional potential of Kenyan infant fecal microbiota in vitro.

Association between dairy intake and risk of incident dementia: the Ohsaki Cohort 2006 Study.

To investigate the association between dairy intake and risk of incident dementia in older adults. A longitudinal analysis of dairy intake with incident dementia was conducted using a cohort study of 11,637 non-disabled Japanese older adults aged ≥ 65years followed-up for up to 5.7years (mean: 5.0years). Data on milk, yogurt, and cheese intake were collected using a validated food frequency questionnaire. Total dairy intake was calculated as the sum of the daily intake of milk, yogurt, and cheese, which was sex-specifically categorized in quintiles. Dementia cases were retrieved from the public long-term care insurance database. Cox proportional hazards model was used to estimate multivariable hazard ratios (HRs) and 95% confidence intervals (95% CIs) for incident dementia. During 58,013 person-years of follow-up, 946 persons developed dementia. In the primary analysis, compare to the lowest quintile of total dairy intake, Q2 showed a slightly decreased incident dementia risk (HR for Q2 vs Q1: 0.90, 95% CI: 0.73-1.10) after fully adjusted for demographic, lifestyle, psychological, and nutritional factors, and diseases history. Compared to non-consumers of milk, those consuming for 1-2 times/month showed a lower risk of incident dementia (fully-adjusted HR: 0.76, 95% CI: 0.57-1.02). Daily yogurt consumers also had a reduced risk (fully-adjusted HR: 0.89, 95% CI: 0.74-1.09). But daily cheese consumers showed an increased risk of dementia (fully-adjusted HR: 1.28, 95% CI: 0.91-1.79). In the sensitivity analysis where we excluded dementia cases ascertained in the first 2years, results were consistent with those from the primary analysis and we further found that yogurt intake might be inversely associated with dementia risk (p for trend = 0.025). Low total dairy intake or low milk intake frequency might be associated with lower incident dementia risk, however daily cheese consumers seemed to have an increased risk. Our study also suggested a possible inverse dose-response association between yogurt consumption and dementia risk, but further studies are needed to confirm whether this benefit was from yogurt intake itself or as a part of a healthy dietary pattern.

Impact of the economic crisis on food consumption of Sri Lankans: An online cross-sectional survey

AimsThis study investigated how the economic crisis has changed Sri Lankans' food consumption. MethodsA web-based cross-sectional survey was carried out in the month of July 2022, using an e-questionnaire based on Google Forms. The questionnaire assessed respondents' socio-demographics, food consumption, and dietary patterns before and during the economic crisis. The changes were compared using descriptive statistics and inferential statistics. ResultsA total of 1095 respondents, aged ≥18 years were included in the survey. There was a significant reduction in the average consumption of main meals per day during the economic crisis (pre: 3.09 ± 0.42, post: 2.82 ± 0.47; P < 0.001). The intake of food items such as rice, bread, and snacks also reduced significantly (P < 0.001). The average daily milk intake frequency decreased from 1.41 ± 1.07 to 0.57 ± 0.80 meals/day (P < 0.001). On contrary, the intake of non-dairy beverages such as malted milk and “plain tea” has increased by several folds. Consumption of fruit and vegetables showed a significant reduction in both frequency and portion size. The consumption of meat, fish, eggs and dhal also decreased in around three-quarters of the study sample. During this period majority (81%) used food coping mechanisms and the most adopted strategy was buying less expensive food. ConclusionSri Lankans' food consumption have been adversely altered due to the country's economic crisis. There has been an overall reduction in the amount and frequency of intake of many common food items.

The Volume of Breast Milk Intake in Infants and Young Children: A Systematic Review and Meta-analysis.

Objective: The objective of this study was to provide global breast milk intake estimates for infants and children from 0 to 3 years old. Materials and Methods: A systematic search of online databases (Embase, MEDLINE, and CENTRAL) was conducted and complemented with a manual search of additional databases (African Journals Online and LILACS), reference lists, and unpublished data. Studies with apparently healthy mothers and their children 0-3 years old worldwide were included. Random effects meta-analyses, subgroup analyses, and meta-regressions were conducted. Results: A total of 167 studies on breast milk intake were identified. The mean daily breast milk intake among all the studies included was 670 mL per day and 117 mL/kg per day. Breast milk intake was influenced by infant age, infant body weight, and breastfeeding practices. The deuterium dilution method tended to yield higher estimates than test-weighing methods. Breast milk intake over time was modeled with a nonlinear meta-regression: breast milk intake (mL/day) = 51-1.4 × days +180 × log(days). When restricting to studies involving healthy term infants exclusively breastfed up to 6 months, breast milk intake was 624 mL per day and 135 mL/kg per day at 1 month, 735 mL per day and 126 mL/kg per day at 3 months, 729 mL per day and 107 mL/kg per day at 6 months, and 593 mL per day and 61 mL/kg per day at 12 months. Conclusions: This review provides global breast milk intake estimates for infants and young children. It demonstrates differences in intakes according to region and measurement method, as well as longitudinal changes over the first year of life.

Patterns and predictors of inter-litter differences in rabbit pup locomotor activity, based on an automatized quantification method

Individual-level sibling interactions in the litter huddle have been studied extensively, especially in the domestic rabbit (Oryctolagus cuniculus). However, little is known about inter-litter differences in pup activity patterns during early postnatal life, in particular regarding the drivers of such variation. In our study on 2–3-day-old rabbit pups, we predicted lower locomotor activity in litters with lower mean body masses on the day of birth (starting body mass) and with lower daily milk intake per pup, possibly constituting a behavioral strategy of pups to cope with associated energetic constraints. For an automatized assessment of pup locomotor activity in the litter huddle, we successfully developed and validated a method based on the quantification of dissimilarities between consecutive frames of video footage. Using this method, we could confirm a U-shaped time course of litter-level locomotor activity, with maximum values shortly before and after the once-daily nursing typical for the rabbit. As predicted, between-litter variation in mean starting body mass and in daily milk intake affected the degree of locomotor activity in the litter huddle, in an interactive way. That is, in litters with heavier starting body masses, pup locomotor activity was greater in pups with an initially higher milk intake, suggesting that only pups with better body condition and a higher energy intake could afford higher levels of activity. This interaction was exclusively apparent during the middle phase of the 24 h inter-nursing interval, when litter activity was low. Shortly before nursing, when pups show higher levels of locomotor behavior in anticipation of the mother's arrival, and shortly after nursing when the pups were more active possibly due to adjustments of their positions in the huddle, activity levels were decoupled from pups’ starting body mass and previous milk intake. Our findings highlight the importance of pup body mass and daily energy intake, two parameters known to be related to maternal characteristics, in shaping inter-litter differences in pup locomotor activity.

Increased Serum Total and Free 25-Hydroxyvitamin D with Daily Intake of Cholecalciferol-Fortified Skim Milk: A Randomized Controlled Trial in Colombian Adolescents

BackgroundThe efficacy of cholecalciferol (vitamin D3) food fortification in low- and middle-income countries near the Equator is unknown. ObjectivesWe examined the effects of providing cholecalciferol-fortified skim milk to adolescents and their mothers on serum total 25(OH)D, free 25(OH)D, and vitamin D–binding protein (DBP) concentrations in a randomized controlled trial. MethodsWe randomly assigned 80 Colombian families each with a child aged 12–14.5 y and their mother 1 L of skim milk daily, either fortified with 2400 IU (60 μg) cholecalciferol or unfortified, for 6 wk. We prescribed 500 mL of milk daily to adolescents; mothers consumed the remainder ad libitum. We estimated intent-to-treat effects as the between-arm difference in the change in serum total and free 25(OH)D and DBP concentrations from baseline to the end of follow-up. Secondary analyses included stratification by baseline characteristics and per-protocol comparisons. ResultsAmong adolescents, fortification effects (95% CI) on serum total 25(OH)D, free 25(OH)D, and DBP concentrations were 5.4 nmol/L (2.1, 8.8 nmol/L), 0.6 pmol/L (−0.2, 1.4 pmol/L), and −416 nmol/L (−944, 112 nmol/L), respectively. Effects on total 25(OH)D were stronger in adolescents with lower DBP concentrations, darker skin, less sunlight exposure, and higher compliance than in their respective counterparts. Fortification increased free 25(OH)D concentrations in high compliers. Among mothers, the effects (95% CI) on total 25(OH)D and DBP concentrations were 4.0 nmol/L (0.6, 7.5 nmol/L) and −128 nmol/L (−637, 381 nmol/L), respectively. There were no adverse events. ConclusionsProvision of cholecalciferol-fortified skim milk increases serum total 25(OH)D concentrations in Colombian adolescents and adult women.

80 To Transfuse or Not to Transfuse: Choosing blood transfusions wisely in young children hospitalized with iron deficiency anemia

Abstract Background Nutritional iron deficiency anemia (IDA) peaks in prevalence at 6to 36 months of age. Children with severe IDA often present to emergency departments and may be hospitalized. Currently, little is known about the management and use of packed red blood cell transfusion in hospitalized children with severe IDA. The current Canadian Paediatric Society (CPS) Practice Point on iron requirements in the first 2 years of life (February 2021) provides guidance on prevention of iron deficiency and iron therapy; however, there is no guidance on the use of blood transfusion in children with severe IDA. Choosing Wisely Canada provides guidance on blood transfusions for adults; however, there are no recommendations on transfusion in children. In contrast, the joint American Society of Hematology and American Society of Pediatric Hematology/Oncology Choosing Wisely Task Force recently published (January 2022) recommendations, including avoiding packed red blood cell transfusion for asymptomatic children with IDA and no active bleeding. Objectives To describe the rate of blood transfusion and characteristics of young children hospitalized with IDA. Design/Methods Data from children, 6 to 36 months, hospitalized with IDA (2001-2020) was abstracted from health records using a standardized data collection form. Eligibility criteria were discharge diagnosis of IDA according to International Classification of Diseases codes, and laboratory evidence of IDA (hemoglobin &amp;lt; 110 g/L and at least two abnormal markers such as MCV, ferritin, transferrin, iron). Descriptive statistics were used. Results Of 79 children hospitalized with nutritional IDA (mean age 18.5 months), 34 (43%) received a blood transfusion. Of those receiving a transfusion, the initial mean hemoglobin was 32g/L (range 17-56g/L). Volume of blood given was: 5mL/kg (n=23, 68%), 10mL/kg (n=8, 23%), and &amp;gt;10mL/kg (n=3, 9%). Two children experienced adverse reactions: allergic reaction; non-hemolytic transfusion reaction. Characteristics of the entire cohort (n=79) were: female (n=51, 65%); previously healthy (n=69, 87%); received iron therapy prior to hospitalization (n=6, 8%); tachycardia on presentation (n=47, 59%); daily milk intake (mean 1638mL, range 236-9000mL). Initial laboratory results (mean) were: hemoglobin 40g/L (range 15-85g/L); MCV 51fL; platelet count 574x109/L; ferritin 3ug/L. Management included dietitian consultation with recommendation to reduce milk intake (n=69, 87%) and all patients received oral iron therapy on discharge (n=79, 100%). No child received intravenous iron therapy during their admission. Conclusion Our findings suggest high rates of blood transfusion among young children hospitalized with iron deficiency anemia. Future research includes examining factors associated with transfusion and practices at other Canadian centres. Our overall aim is to help inform future CPS and Choosing Wisely Canada recommendations.

Health Risk of Ingested Heavy Metals in Fluidized Canned Milks: Are We Drinking Heavy Metals?

This study examined the heavy metal level of canned milk consumed in Calabar, Cross River State, as well as the health risks linked to it. Peak Milk, Three Crown, Coast, Nunu, Cowbell, and Olympic milk types were chosen for research. During the digestion of samples, 0.5 mol of nitric acid was added to the sample and heated. The heated liquid was slowly heated with 2.5 mL of 70% HClO4 until a dense white vapor was formed. After cooling the mixture, 10 mL of deionized water was added and the solution was boiled to expel the fumes. The heavy metals were screened using an atomic absorption spectrophotometer. All milk samples contained eight heavy metals: iron (Fe), copper (Cu), zinc (Zn), arsenic (As), lead (Pb), cadmium (Cd), manganese (Mn), and nickel (Ni). Peak Milk and Nunu both had Cd. Nickel was found in various quantities in Coast, Cowbell, Nunu, and Olympic. Coast, Nunu, and Cowbell samples all contained Mn. Nunu and Cowbell both tested positive for lead. Peak Milk did not contain copper (Cu). Standard models for daily consumption of different heavy metals, such as Pb in Nunu (3.7E − 03) and Cowbell (−1.8E − 03), were used to construct the health risk evaluations. Peak Milk, Three Crown, and Nunu had daily Cd intakes of (4.5E − 06), (2.2E − 05), and (4.5E − 06), respectively. Coast (3.2E − 02), Nunu (5.1E − 02), Cowbell (1.9E − 02), and Olympic (3.8E − 02) have different daily Ni intakes. Peak Milk (1.1E − 01), Three Crown (2.2E − 01), Coast (1.6E − 01), Nunu (7.1E − 01), Cowbell (1.4E − 01), and Olympic (1.1E − 01) have different daily intakes of Ar. Peak (6.0E − 04), Three Crown (8.0E − 04), Coast (6.0E − 04), Nunu (7.0E − 04), Cowbell (8.0E − 04), and Olympic (6.0E − 04) had different daily Zn intakes. Daily Fe intakes of Peak Milk was (1.6E − 01), Three Crown was (1.6E − 01), Coast was (1.4E − 01), Nunu was (1.4E − 01), Cowbell was (2.4E − 01), and Olympic was (1.8E − 01). Cu intakes per day for Three Crown, Coast, Nunu, Cowbell, and Olympic were (6.0E − 03), (4.0E − 03), (2.0E − 03), (2.0E − 03), and (4.0E − 03), respectively. Coast, Nunu, and Cowbell had daily Mn intakes of (2.0E − 04), respectively. The total hazard index (THI) and the target hazard quotient (THQ) were also calculated. Peak Milk (1.7E − 01), Three Crown (3.4E − 01), Coast (2.8E − 01), Nunu (1.9E − 01), Cowbell (2.4E − 01), and Olympic (2.3E − 01) induced cancer risks, accordingly. According to the findings, the risk of drinking milk is relatively considerable when compared to the acceptable limit.

Trends in Milk &amp; Dairy products consumption in 4th year MBBS students and assessing their bone densities

Background: Milk and dairy products provide a package of essential nutrients despite the established benefits for bone health. Aim: To find trends in medical students of a senior class about their consumption of milk and the dairy products and measuring their bone densities, with dexa-scan, to assess risk of osteoporosis. Methodology: This cross sectional study was conducted online through Google classroom, in order to engage students and teachers for research activities and teaching methodologies at Lahore Medical &amp; Dental College, Lahore, Pakistan for four months. After having detailed review of literature about the topic, we developed a simple structured questionnaire, which was uploaded to Google classroom assignment page and was kept ungraded form. Results: The pilot research group of 27 students were trained first online and then through the Google classroom collected the data from rest of the students of the same class. Data entry and cleaning was done through Google classroom. Descriptive statistics including frequency and percentage were used to represent the data. Data was analyzed statistically through Google Classroom Analyzer. Conclusion: Concluded from this study that the medical students who participated in this study were not taking the recommended daily amount of milk or dairy products. Recommendations: To achieve the recommended daily milk and dairy products serving consumption, family involvement recommended that specifically address support for promoting daily milk and dairy products intake among adolescents. Keywords: Milk, dietary habits, life style, medical students, online teaching, bone scan