The experimental approach is crucial for investigating the seismic performance and damage process of underground structures. Considering the shortcomings of the 1-g, centrifuge shaking table and monotonic displacement pushover tests, a large-scale cyclic displacement pushover test method is proposed based on the soil-underground structure dynamic interaction and seismic performance quantification system. Taking a two-story three-span subway station structure as the prototype, the cyclic displacement pushover test device was designed for a 1/7-scale multi-story subway station based on the seismic response characteristics of underground structures. The corresponding numerical simulations and experiments were conducted. Typical numerical results (including the seismic damage process, capacity curves of the structural columns, and strain response) and test results (the macroscopic phenomenon of structural damage development, strain response, and deformation response) are interpreted. The results show that the proposed cyclic displacement pushover test is better than the monotonic displacement pushover test, the damage process of the tested station structure conforms to the description of the inter-story drift ratio (IDR) quantification system of seismic performance. Meanwhile, the column has greater strain amplitudes than other components, and the column strain curves reach their peaks before other components. Furthermore, the tested station structure has a similar damage pattern to the Daikai subway station. The reliability and feasibility of the proposed cyclic displacement pushover test method are verified.