Dahliae Race Research Articles

Resistance of Tomato Genotypes to Four Isolates of Verticillium dahliae Race 2

Eight tomato (Lycopersicon esculentum Mill.) genotypes were evaluated based on shoot dry weight for resistance to four isolates of Verticillium dahliae Kleb. race 2 in two greenhouse seedling experiments. The race 2 isolates, obtained from North Carolina, Brazil, and Spain, demonstrated no differences in pathogenicity on the eight lines tested, thus precluding the identification of a third V. dahliae race in this collection. However, highly significant differences in virulence were observed among the isolates. The Brazilian isolate was the most virulent. No tomato genotype showed resistance comparable to that conferred by the single dominant Ve gene to V. dahliae race 1. While all tomato lines were susceptible to all race 2 isolates tested, there were significant differences in susceptibility equal to differences in levels of resistance. IRAT L3, Morden Lac, Okitsu Sozai, and `UC82' significantly outperformed the lowest ranking line XXIV-a. `Earlypak 7', Morden Mel, and Philippine 2 performance was statistically indistinguishable from that of either the highest- or lowest-ranked lines. Genetic diversity in the host and pathogen and environmental conditions favoring the pathogen likely contributed to the genotype × isolate interactions observed in Expt. 1. These results suggest using diverse isolates when screening for improved race 2 resistance.

Appartenance Raciale et Pathogenie Comparée de Quelques Isolats de Verticillium dabliae (Kleb.) Obtenus a Partir de Tomateś Résistantes au Maroc

Abstract Pathogenecity and race classification of some isolates of V. dahliae from resistant tomato in Morocco In Morocco, the cv. H 204, resistant to Verticillium dahliae race 1 is widely used under plastic tunnels. Use of this cultivar controlled the disease for many years but recently a high incidence of Verticillium on this hybird was obsereved. V. dahliae was isolated from 90 % of the wilted plants.The other 10 % were infected by F. oxysporum f. sp. lycopersici alone (7 %) or in association with V dabliae (3 %). The inoculation of the Verticillium susceptible cv. Vemone and of the race 1 resistant cv. H 204 by 120 isolates obtained from the resistant hybird, showed that (69 %) of the isolates are race 1 and 31 % are race 2. There are different virulence levels of V. dahliae race 2 both on the susceptible cv. Vemone and on the race 1 resistant cv. H 204. Race 2 on the average was less virulenton the susceptible than on the resistant cultivar.

Breeding for Resistance to Verticillium dahliae Race 2 of Tomato in North Carolina1

Abstract Parental, F1, F2, and backcross generations of tomato (Lycopersicon esculentum Mill.) lines Morden MEL2668170G (MEL) and ‘Walter’ (WA) were screened as seedlings for resistance to verticillium wilt (Verticillium dahliae Kleb.) race 2. Disease reaction means, based on the number of leaves and cotyledons with symptoms 5.5 weeks after inoculation, were 2.7 for MEL and 4.6 for WA. Resistance in MEL appeared to be recessive with narrow sense heritability estimated at 0.25 or less. Disease severity was sometimes inadequate to avoid the selection of escapes in advanced generations. Analysis of F3 progenies, however, indicated 3 genes or less might be involved. Results suggest race-2 resistance can be maintained in advanced generations by the elimination of highly susceptible plants and by progeny testing to insure recovery of the most resistant individuals rather than escapes.