Soil Water δ18O Research Articles

The relationship of δD and δ18O in soil water and its implications for soil evaporation across distinct rainfall years in winter wheat field in the North China Plain

Soil evaporation plays a key role in regulating local climate and water loss. Stable isotope ratios of water (²H/¹H and ¹⁸O/¹⁶O) are effective tracers for studying water flux. This study examines three isotope-based indicators deuterium excess (d-excess), the slope of the soil water evaporation line (SEL), and line-conditioned excess (lc-excess) across three wheat growing seasons: wet, ordinary, and dry years. The influencing factors of d-excess, SEL, and lc-excess, respectively, soil, vegetation, and meteorology, were analyzed using various methods. Wheat yields varied significantly, reaching 6.69 t ha⁻¹ in wet years, 8.66 t ha⁻¹ in dry years, and 9.28 t ha⁻¹ in ordinary years. The lc-excess was highest in ordinary years, and d-excess peaked during dry years. A negative correlation between d-excess and SEL slope, and between lc-excess and SEL slope, was observed in dry and ordinary years (P<0.05), but not in wet years (P>0.05). Multivariate regression showed that net radiation (Rn) was the primary factor influencing SEL, contributing 54.19 %, 11.58 %, and 29.27 % in wet, dry, and ordinary years, respectively. Leaf area index (LAI) was the most significant factor affecting lc-excess (37.91 % in wet years, 32.22 % in dry, and 30.92 % in ordinary years). Vapor pressure deficit (VPD) affected d-excess in wet and ordinary years, while air (Ta) and soil temperature (Ts) were key in dry years. Variation partitioning revealed meteorological factors primarily influenced SEL, lc-excess, and d-excess in wet years, while soil, vegetation, and climate interactions had greater effects in dry and ordinary years. The lc-excess, integrating multiple factors, is a better indicator of soil evaporation than SEL.

Water uptake patterns and rooting depths of Tamarix ramosissima in the coppice dunes of the Gurbantünggüt Desert, China: a stable isotope analysis.

Tamarix ramosissima has an important role in stabilizing sand dunes in desert ecosystems. Understanding the water use strategies of T. ramosissima is essential to understand its adaptations on coppice dunes. We utilized the stable isotopes δ2H and δ18O in soil water, groundwater, and xylem water to identify monthly differences in water sources. Additionally. we explored rooting depth using 2H2O as an artificial tracer. In May, T. ramosissima derived 75% of its water from shallow and middle soil layers. In July, it absorbed 90% water from middle and deep soil layers. In August and September, it acquired approximately 80% of its water from deep soil layers. The labelling using 2H as an artificial tracer indicated that the root system of T. ramosissima could reach depths >500 cm in the coppice dunes. 2H absorption was observed at depths of 100, 200, 300 and 400 cm. Soil water is the dominant water source for T. ramosissima in the coppice dunes because groundwater is at depths >30 m. The flexible water-use strategies of T. ramosissima enable it to effectively utilize different available water sources to adapt to the arid environment. These findings improve our understanding of water uptake patterns and drought adaptation strategies of T. ramosissima in the coppice dunes of desert ecosystems.

Increasing effect of biocrusts on evaporation is evidenced by simulating evaporation and diffusion experiments and water stable isotope analysis

Evaporation, one of the main components of the water and energy balance between soil and atmosphere, plays a key role in hydrological processes particularly in semi-arid and arid climate regions. As critical living organism communities inhabiting the soil-atmosphere boundary, biocrusts are capable of modifying near-surface soil properties and structure in drylands. However, the roles of biocrusts in mediating soil evaporation still remain greatly controversial, and the literature yet lacks in a systematic assessment. In this study, evaporation experiments were conducted under simulated isothermal and non-isothermal conditions for bare soil and three types of biocrusts (cyanobacterial, moss, and their mixture) sampled from a semi-arid climate region of the Chinese Loess Plateau. During the evaporation, soil temperature and moisture were measured at depths 2, 5, 10, and 15 cm for those biocrusts and bare soil. Their water vapor diffusion process and stable isotopic composition were also analyzed in the laboratory. We found that the measured evaporation yielded 4.9%, 17.5%, and 31.6% higher average evaporation rate for cyanobacterial, cyano-moss, and moss crusts, respectively, when compared to the bare soil. In comparison to bare soil, all types of biocrusts markedly reduced the rate of temperature rise, prolonged the duration of wetting time, and delayed the formation of dry surface layer. The largest regulating effects on soil temperature and moisture were found in the moss crusts instead of cyanobacterial or cyano-moss crusts. The increasing evaporation in biocrusts was attributed to their higher fine particle content, lower bulk density, and greater water holding capacity. Moreover, the water vapor diffusion rate and diffusivity of biocrusts were 18.2% and 16.4% higher than those of bare soil, respectively, which is attributable to the increased total porosity and better soil structure. Additionally, the isotopic composition of soil water (0–50 cm) in bare soil and moss crusts was distributed below the local meteoric water line. The mean δ18O and δ2H in soil water gradually decreased with increasing soil depth, and the moss crusts were more isotopically enriched than bare soil. Based on the enhanced soil evaporation rate, improved vapor diffusivity, and enriched isotopic composition of biocrusts in contrast to bare soil, we conclude that biocrusts exacerbate vapor transport and evaporative loss, and consequently they are crucial surface cover in rebalancing surface ecohydrological and biochemical processes in global drylands. These findings improve our understanding of the complexity of dryland hydrology, and a full consideration should be given in future biocrust studies.

Seasonal transpiration dynamics and water use strategy of a farmland shelterbelt in Gurbantunggut Desert oasis, northwestern China

Farmland shelterbelts provide an ecological barrier to oases, but their growth is inhibited or even dies under water scarcity. Therefore, it is necessary to understand the response of farmland shelterbelts to environmental changes for their own survival in order to promote the long-term healthy development of agroforestry systems and improve water management in oases in the northwest arid region. In this study, we measured the sap flow and environmental factors to determine the transpiration dynamics and its influencing factors. The seasonal variations in water use strategy of Populus alba var. pyramidalis Bunge were identified by using stable isotopes of δ18O in xylem, soil water and groundwater coupled with the Iso-Source model. The results showed that the transpiration of the populus was significantly higher in the growing season (more than 80% of the total transpiration) than in the non-growing season, and the transpiration was closely related to air temperature, vapor pressure deficit, soil water content, and soil temperature. The water use strategy had seasonal variations. The populus obtained water predominantly from 0–40 cm soil water (approximately 75%) in the wet spring and then switched to deeper water sources (40–150 cm soil water and groundwater) as the season progressed. The contribution of deeper water sources increased to 84–92% in summer and autumn. This suggested that Populus alba var. pyramidalis Bunge has ecological plasticity, as it could explore deeper water sources as the water stress increased. The ability to switch rapidly among different water sources could put a plant at an advantage if competition for water occurs within the ecosystem. Our findings can provide insights into temporal transpiration dynamics as well as the strategy adopted by farmland shelterbelts to counteract the drought stress associated with the shallow soils in desert oasis ecosystems.

Water Uptake Characteristics of Stipa bungeana Trin: Affected by Subsidence in the Coal Mining Areas of Northwest China

Revealing the water use pattern of plants influenced by coal-mining-caused land subsidence is crucial to understand plant–water interactions and guide ecological restoration. However, available information on herbaceous plants, the dominant species in most arid and semi-arid regions with abundant coal resources, remains inadequate. We investigated the water use patterns of Stipa bungeana Trin. by measuring soil water content, root distribution, and stable isotopes of hydrogen (δ2H) and oxygen (δ18O) of soil water and plant stem water both before and after a rainfall event. The results revealed that prior to rainfall, both areas exhibited a low soil water content with no discernible difference in soil drought. However, the soil waters δ2H and δ18O were found to be more enriched at varying depths within the subsidence area, indicating a heightened level of soil evaporation. Both soil water content and soil water isotopic composition responded sensitively to rainfall, with rainfall primarily replenishing the shallow layer (0–20 cm), thereby reflecting an infiltration mode dominated by piston flow. More water seeped into deeper soil layers in the subsidence area compared to the non-subsidence area, with more preferential flow. Before rainfall, the sources of plant water uptake were consistent both at shallow and deep soil layers, implying that the proportion of water uptake gradually decreased with increasing depth. After rainfall, the sources of plant water uptake differed slightly between the two soil layers. The plants in non-subsidence and subsidence areas dominantly extracted soil water at depths greater than 10 cm and 20 cm, respectively. The root system in the subsidence area was more developed than that in the non-subsidence area. Plant water uptake was primarily influenced by the spatial distribution of roots, as well as the post-rainfall water distribution, regardless of whether they were in the subsidence area or not. Although land subsidence affected soil water transport, the water uptake pattern of Stipa bungeana Trin. was similar before and after rainfall, indicating the adaptive growth of plants through their roots in the subsidence area. The high adaptability of herbs such as Stipa bungeana Trin. makes them a viable option for vegetation restoration in subsidence areas. This study has significant implications for evaluating plant–water relationships in subsidence areas due to coal mining, thereby providing a fundamental basis and valuable reference for ecological restoration and management strategies within such affected regions.

The competitive relationship of scrub plants for water use in the subalpine zone of the Qilian Mountains in China.

Samples of scrub plants and soil were collected from May to October 2019 in the subalpine scrub zone of the Qilian Mountains. Based on measured oxygen isotope values (δ18O) in plant xylem water and soil water, the multivariate linear mixed model (IsoSource) and the proportional similarity index (PS index) were used to analyze the using proportion for each potential water source and the competition relationship for water use of scrub plants in different growing periods and habitats. The results showed that the soil water content gradually decreased with increasing depth of the soil layer, with the maximum value in the soil layer of 0-10cm. Most of the scrub plants mainly used soil water in the soil layer of 0-30cm during the different periods of growing season, but Salix sclerophylla Anderss. and Salix oritrepha Schneid. on the semi-sunny slope habitat mainly used soil water in the soil layer of 40-80cm during the middle period of growing season (July-August), with the proportion of 59.5% and 52.1%, respectively; and Potentilla fruticosa Linn. and Salix cupularis Rehd. on the semi-shady slope habitat mainly used soil water in the soil layer of 30-60cm during the early period of growing season (May-June), with the proportion of 61.1% and 49.7%, respectively. The competition relationships of scrub plants for water use varied during different periods of growing season (P < 0.05). On the semi-sunny slope habitat, they were fiercest for Salix cupularis Rehd. and Rhododendron thymifolium Maxim., Potentilla fruticosa Linn., and Salix sclerophylla Anderss. during the early period of growing season; Salix cupularis Rehd. and Rhododendron thymifolium Maxim. during the middle period of growing season, and Salix sclerophylla Anderss. and Salix oritrepha Schneid. during the end period of growing season (September-October). On the semi-shady slope habitat, they were fiercest for Salix oritrepha Schneid. and Caragana jubata (Pall.) Poir. during the early period of growing season; Rhododendron przewalskii Maxim. and Rhododendron thymifolium Maxim. during the middle period of growing season; and Salix cupularis Rehd. and Salix oritrepha Schneid. during the end period of growing season. This study reveals the competitive relationship of scrub plants for water use in the subalpine zone and their response to environmental changes, so as to provide theoretical references for the ecological conservation in the ecologically fragile areas of the Qilian Mountains.

Dynamic hydrological niche segregation: How plants compete for water in a semi-arid ecosystem

Hydrological niche segregation (HNS), specifically the variation in root water uptake depth among coexisting species, is an understudied area of research. This is especially the case in semi-arid ecosystems, such as China’s Loess Plateau (CLP), where seasonal aridity necessitates adaptive water use strategies among plant species. In this study, we conducted a two-year investigation to understand the water sources and intrinsic water use efficiency (WUEi) of four coexisting plant species: Populus simonii (tree), Caragana korshinskii and Salix psammophila (shrubs), and Artemisia ordosica (semi-shrub). We analyzed the isotopic compositions of xylem and soil water (δ2H and δ18O) and leaf δ13C to identify the water sources and WUEi, respectively, of each species. We then used the nicheROVER model to quantify HNS based on the variations in xylem water δ2H and δ18O. Our results show that the four co-existing species occupied distinct positions on a hydrological niche axis, delineated by their respective water sources and disparate WUEi. The tree P. simonii exhibited a preference for deep soil water and demonstrated a high WUEi. Both shrubs, S. psammophila and C. korshinskii, utilized intermediate and deep soil water, respectively, and with comparable WUEi. Conversely, the semi-shrub A. ordosica relied on shallow soil water and displayed a low WUEi. These differences in water sources and WUEi led to HNS between A. ordosica and the other three species in a relatively wet year. However, in a relatively dry year, HNS between A. ordosica and the other three species contracted and WUEi increased as species increased the use of deep soil water. Overall, these results demonstrate that HNS is a dynamic phenomenon that varies on at least an annual basis. It expands and contracts as plants regulate their water uptake and loss in response to changing soil moisture conditions.

Exploring recharge mechanisms of soil water in the thick unsaturated zone using water isotopes in the North China Plain

Exploring the groundwater recharge mechanisms in regions with thick unsaturated zones can enhance our understanding of intricate groundwater processes, especially in intensive agricultural regions such as the North China Plain (NCP). For example, to better answer the question of how deep soil water can be recharged, it is of utmost importance to illustrate the recharge mechanisms of groundwater under different land use types in the NCP. This study collected soil samples from four boreholes up to 18 m deep covering farmland and orchards with different stand ages and measured soil water content (SWC), stable and radioactive isotopes (δ18O, δ2H and 3H) of both soil water and groundwater. Results indicated that the movement of water through the deep vadose zone of soils was primarily piston flow based on a soil tritium profile. The infiltration rate was estimated to be 32.2 mm yr−1 based on the tritium peak method. The variations of soil water δ18O and line control excess (lc-excess) indicated that the orchards had lower evaporation effects and a higher precipitation offset than those in farmland soils. Furthermore, the deep soil water was replenished by intensive precipitation events (from July to September). The great soil water deficit and decreased deep drainage under pear orchards is usually closely related to huge water consumption by evapotranspiration (ET) via root uptake, which explains the increase of ET in pear orchards with increasing stand age. These findings have provided substantial insights into groundwater resource management in similar regions with limited water resources and recharge modeling for regions covered with thick unsaturated zones.

Precipitation δ18O paced the seasonal δ18O variations of terrestrial snail body water and shells in the East Asian monsoon region

Terrestrial snails are sensitive to climate changes and their shell's oxygen isotope composition (δ18Oshell) is widely used in studies of paleoclimate reconstructions. However, the interpretation of δ18Oshell remains complex due to the combined effects from different factors such as precipitation δ18O (δ18Op), relative humidity (RH), and temperature. Furthermore, no systematic studies have addressed the transformation of oxygen isotope signals from rainfall to the aragonite shell via snail body water. Here we present the results of Cathaica fasciola body water δ18O (δ18OBW) and its content, which were sampled every two days throughout the growing season in 2021 in Xi'an. Meanwhile, the soil water δ18O (δ18OSW), δ18Op, rainfall amount, RH and temperature were also simultaneously monitored, and two high-resolution (at ∼0.3 mm interval) δ18Oshell series were obtained from two live snails collected in September and October 2021, respectively. These data reveal that the δ18OBW mimic the variations of δ18Op, and they are positively correlated (r2 = 0.84) for precipitation events and the correlation coefficient only increases slightly (r2 = 0.88) when RH is included in the multivariate analysis. During non-precipitation intervals, there is an obvious negative correlation between δ18OBW and RH. Moreover, the variations of two δ18Oshell series are broadly consistent with the theoretically calculated δ18Oshell from δ18OBW/δ18Op at seasonal scale. Collectively, this study demonstrates that δ18Op is the first-order control on the seasonal variations of δ18OBW and δ18Oshell, while RH play its role during non-precipitation periods. Importantly, this study lays good foundation for reconstructing terrestrial seasonal distribution of precipitation and extreme rainfall events using fossil shells in the geological past.

Hydrogen and Oxygen Isotope Fractionation Effects in Different Organ Tissues of Grapes under Drought Conditions.

A study of different grapevine tissues and organs (root, stem, leaf, fruit) water isotope fractionation models from high-quality wine grapes produced in the Helan Mountains, a key wine-producing area in northwestern China, was undertaken. Results showed that δ2H values of local groundwater sources were more negative than rivers and precipitation. Soil water δ2H and δ18O values were significantly higher than those of other environmental water sources. Water from the soil surface layer (0-30 cm, δ2H and δ18O values) was more positive than the deeper layer (30-60 cm), indicating that soil water has undergone a positive fractionation effect. δ2H and δ18O values of tissues and organs from different grape varieties followed a similar pattern but were more negative than the local atmospheric precipitation line (slope between 4.1 to 5.2). The 2H and 18O fractionation relationship in grapevine organs was similar, and 18O has a higher fractionation effect than 2H. δ2H and δ18O values showed a strong fractionation effect during the transportation of water to different grape organs (trend of stem > fruit > leaf). This study showed that 18/16O fractionation in grapes is more likely to occur under drought conditions and provides a theoretical basis to improve traceability accuracy and origin protection of wine production areas.

Source of soil water in cold regions based on stable isotope tracers

Soil water plays a key role in vegetation growth and ecological stability in cold regions. The soil water sources in the Qilian Mountains of the northeastern Tibetan Plateau have been quantified based on stable isotope data (δ2H and δ18O) of 1913 samples. The results indicated that δ18O of soil water ranged from ˗14.13–11.21‰, with an average of ˗6.78‰, and on a spatial scale, it increased gradually from the southeast to the northwest, where it became more positive. The stable isotopes of soil water are affected by evaporation, leading to a lower slope and interception of the evaporation line: δ2H = 3.23 δ18O–32.51 (R2 = 0.71, p < 0.01). The relationship between soil water and other water bodies showed that the soil water was mainly replenished by precipitation and ground ice meltwater. End-member mixing analysis (EMMA) showed that precipitation and ground ice accounted for approximately 91 % and 9 % of soil water, respectively, during heavy ablation. In the 0–20 cm, 20–40 cm, 40–60 cm, 60–80 cm, and 80–100 cm soil layers, the contributions of ground ice meltwater to soil water were 0 %, 3 %, 7 %, 13 %, and 18 %, respectively. Notably, the contribution of ground ice meltwater to soil water gradually increased with elevation, whereas that of precipitation decreased. This study has obtained relevant data for the cold region, where it is typically difficult to conduct observations and sampling. More importantly, it analyzes the source and influencing factors of soil water in cold regions using the stable isotope method, which is of great significance for the study of the water cycle in cold regions.

A Seasonally Varying Tree Physiological Response to Environmental Conditions: Results From Semi‐Arid China

AbstractDrought is the primary limiting factor for tree growth in water‐stressed regions through its effects on xylem development. In this study, we developed intra‐annual series of tree‐ring stable carbon (δ13C) and oxygen (δ18O) isotopes from Chinese pine (Pinus tabuliformis) to investigate the ecophysiological responses of trees to drought during the growing season in a climatic transition zone between arid and semi‐arid areas in north‐central China. Distinct intra‐annual patterns of δ13C and δ18O were revealed, with average annual amplitude of 0.26 and 2.72‰, respectively. Tree‐ring δ13C values increased from the early‐ to mid‐growing season, peaked in the mid‐to‐late‐growing season, and decreased at the end of the growing season. In contrast, δ18O values continuously decreased throughout the growing season. As expected, drought significantly increased δ13C and altered its intra‐annual pattern, particularly toward the end of the growing season. However, there was no significant difference in the average intra‐annual pattern in δ18O between dry and wet years. The tree‐ring δ18O at the end of the growing season was significantly correlated with the September to October average soil water δ18O, as simulated by a global isotope‐enabled climate model, which is consistent with the high proportion of exchangeable oxygen atoms predicted by previous studies for cellulose synthesis in the late growing season.

Variability in the Hydrological Processes of Six Typical Woodlands Based on Stable Isotopes in Subtropical Regions in Central China

Changes in woodland characteristics induced by plants and soil greatly affect soil hydrological processes. Stable isotope technology and indoor soil moisture characteristic experiments were conducted at three rainfall levels (3.6, 23.5, and 49.8 mm) to investigate the hydrological processes under six woodlands (two pure forests and four mixed forests). The main influencing factors contributing to these changes were identified in a low mountain and hilly region in central China. The soil waterline equation in this area was soil water δD = 5.626 δ18O − 16.791 (R2 = 0.798). The slope and intercept in the soil waterline equation were smaller than the atmospheric waterline equation. From a temporal perspective, the soil moisture content varied in the same trend under different rainfall events, with the maximum and minimum values on the first day after rainfall and the day before rainfall, respectively. However, an overall trend that first increased and then decreased was observed. From a spatial perspective, the soil moisture content increased with soil depth, and the increase rate was in the order of 0–20 cm and 20–40 cm in different soil layers. The soil moisture content in mixed conifer broadleaved woodlands was high. The soil water δD and δ18O in mixed conifer broadleaved woodlands and underground soil were relatively depleted. The effects of soil water-holding capacity, particle size composition, slope, canopy closure, and other factors on soil hydraulic parameters were comprehensively analyzed. The results showed that the extremely coarse sand (1–2 mm) particle content was the main parameter affecting soil-saturated hydraulic conductivity Ks, whereas the slope was the main factor affecting soil water δD and δ18O. In needle-leaved forests, the soil water infiltration form was a rainwater and soil water mixture downward diffusion, whereas the rainwater replaced the original soil water in the needle and mixed conifer broadleaved forests.

Understanding the soil and plant water hydrological cycle in a typical wetland under harsh natural conditions in the Shigatse area of the Tibetan Plateau

AbstractIn cold alpine regions, ecohydrological feedback to climatic anomalies can provide valuable insights into ecosystem functions in the context of alarming climate change projections. However, the role of vegetation in the hydrological cycle of alpine wetlands remains unclear. We used water stable isotopes (δ18O and δD) supplemented with δ13C to investigate wetland soil isotope distributions and plant water uptake patterns. The isotope values of the soil profiles were affected by seasonal factors. During cold and dry periods, the isotopes within the soil profile showed a strong evaporation effect, and during warm and humid periods, the isotopes indicated the mixing of multiple water sources. The isotopes show larger variations in the interior of the wetland than in the periphery. The carbon dioxide and methane released by the wetland may cause a negative shift in the δ18O and δD isotopes of soil water. Rose willow and Sophora moorcroftiana can obtain water from both the surface and deep layers to cope with different water stresses. The dominant water is mainly obtained from the surface layer during cold and dry periods, whereas water from deeper layers can also be abstracted during warm and wet periods for rose willow growth. These findings will provide a better understanding of soil and plant water hydrological cycles under harsh natural conditions in cold alpine regions and could offer guidance to local governments for the conservation of this extreme wetland ecosystem.

Uncorrected soil water isotopes through cryogenic vacuum distillation may lead to a false estimation on plant water sources

Abstract Successful use of stable isotopes (δ2H and δ18O) in ecohydrological studies relies on the accurate extraction of unfractionated water from different types of soil samples. Cryogenic vacuum distillation (CVD) is a common laboratory‐based technique used for soil water extraction; however, the reliability of this technique in reflecting soil water δ2H and δ18O is still of concern. This study examines the reliability of a newly developed automatic cryogenic vacuum distillation (ACVD) system. We further assessed the impacts of extraction parameters (i.e. extraction time, temperature and vacuum) and soil properties on the recovery of soil water δ2H and δ18O for the ACVD and traditional cryogenic vacuum distillation (TCVD) systems. Finally, we investigated the potential influence of CVD (ACVD and TCVD) technique on the prediction of plant water uptake through a sensitivity analysis. Both ACVD and TCVD similarly extracted water from the rewetted soils, but none of the CVD systems successfully recovered the isotopic signatures of doped water from soil materials. Mean δ2H offsets of extracted soil water were −2.6 ± 1.3‰ and −2.4 ± 1.7‰ for ACVD and TCVD, respectively, while mean δ18O offsets were −0.16 ± 0.14‰ and −0.39 ± 0.37‰. The isotopic offsets of CVD systems were positively correlated with soil clay content, and negatively correlated with soil water content. Using corrected soil data (with CVD offsets) could improve the prediction of plant water uptake based on its high correlation with the environmental factors. This study identifies the isotopic offsets of CVD systems (i.e. ACVD and TCVD) and provides possible solutions for better predicting plant water sources. Even though, the wide use of CVD techniques probably induce noticeable uncertainties in the prediction of plants water uptake depths. The dataset of soil water extraction in this study will have implications for the technological development of CVD techniques.

Forest Plant Water Utilization and the Eco-Hydrological Regulation in the Karst Desertification Control Drainage Area

Subtropical forests in southwestern karst areas are the top priority for ecosystem restoration, as studying the water absorption strategies of the major plants in these regions is crucial to determining the species distribution and coexistences within these seasonal subtropical forests, which will help us to cope with the forest ecosystem crisis under future climate change. We used the stable isotope ratios (δD and δ18O) of tree xylem and soil water to assess the seasonal changes in the water use patterns and hydrological niche separations of four dominant tree species in seasonal subtropical forests in southwestern karst areas. The results showed that the soil water’s isotopic composition varied gradiently in the vertical direction and that the variation of the soil water’s isotopic composition was greater in the shallow layer than in its depths. Juglans regia (HT) mainly depended on soil water at a depth of 30–60 cm (41.8 ± 6.86%) and fissure water (32.5 ± 4.21%), while Zanthoxylum bungeanum Maxim (HJ) and Eriobotrya japonica Lindl (PP) had the same water use pattern. In the dry season, HT competed with HJ and PP for water resources, and in the rainy season, HJ and PP competed with Lonicera japonica (JYH), while HJ competed with PP all the time. JYH and HT were in a separate state of hydrologic niche and they did not pose a threat to each other. Coexisting trees are largely separated along a single hydrological niche axis that is defined by their differences in root depth, which are closely related to tree size. Our results support the theory of hydrological niche isolation and its potential responses in relation to drought resistance. This study provides a method for determining more efficient plant combinations within karst forest vegetation habitats and its results will have important implications for ecosystem vegetation restoration.

Multi-species plantation intensifies soil water competition and groundwater depletion in a water-limited desert region

Multi-species plantation has long been applied in forestry and landscape restoration to provide better ecosystem services and functions. However, the differences in water-use patterns between mixed and pure plantations are poorly understood, especially in the arid/semi-arid artificial desert ecosystems. The stable isotope compositions (δ2H and δ18O) of rainwater, xylem water, soil water, and groundwater were analyzed over three growing seasons (2019–2021) to identify the water-use patterns of Artemisia desterorum Spreng (A. desterorum), Amorpha fruticosea (A. fruticosa) and Pinus sylvestris var. mongolica (P. sylvestris) in pure and mixed plantations in the Mu Us Sandy Land. Seasonally, there was a transference of water-use pattern from deep (moist layer and groundwater) to shallow water sources (active and stable layers) during the transition period between the dry and rainy season for both pure and mixed A. fruticosa and P. sylvestris, which was attributed to dimorphic root systems of arbor and shrub in the water-limited region. However, A. desterorum with shallower roots predominately absorbed water from the active and stable layers across the whole growing season regardless of planting pattern. Annually, the three plant species in pure stands extracted water predominantly from the stable layer (36.97%–46.16%), followed by the active layer (23.55%–36.98%). In the mixed stand, however, all the plants absorbed water mainly from the active layer (33.04%–39.44%), followed by the stable layer (30.46%–33.68%), suggesting that multi-species plantation intensifies shallow soil water competition. Additionally, the proportional contribution of groundwater to plant water uptake under mixed plantation was higher than the pure plantations, causing faster groundwater decline in the mixed stand (0.29 m yr−1) compared to pure stands (0.11–0.20 m yr−1). Our study demonstrates that multi-species plantation in the water-limited desert region intensifies soil water competition and groundwater depletion and thus adversely affects sustainable development goals.

Water competition among the coexisting Platycladus orientalis, Prunus davidiana and Medicago sativa in a semi-arid agroforestry system

Agroforestry has been widely used for the ecological restoration construction of semi-arid regions of the Chinese Loess Plateau. Investigating plant water sources is crucial for understanding the key ecohydrological processes of plant root water uptake and the water relations between coexisting plants. Clarifying the plant water use strategies can help to assess the sustainability of the vegetation restoration in agroforestry systems and provide references for the vegetation management. Thus, we investigated the water use characteristics of plants in an agroforestry system consisting of Platycladus orientalis, Prunus davidiana and Medicago sativa. The δD and δ18O of plant stem water and soil water in the 0–300 cm layer were measured. The MixSIAR model and proportional similarity (PS) were used to quantify the proportion of soil water absorbed by the plants in each layer and the hydrological niche overlap of plants. The results showed that the combined effects of rainfall recharge and evapotranspiration (ET) loss resulted in a decrease in soil water content (SWC) for the agroforestry system with increasing soil depth, and the SWC of the deep layer (80–300 cm) had minor variability (6.1–7.1%) throughout the study period. During the study period, due to the deep soil desiccation, P. orientalis, P. davidiana and M. sativa mainly utilized water from shallow (0–80 cm) soil layers, with a contribution rate of 65.4 ± 8.3%, 64.9 ± 7.7% and 73.0 ± 0.2%, respectively. The three coexisting plants relied on the unstable shallow soil water (recharged by rainfall) while the water use proportion of the deep soil layer was consistently low. This implies that the three plants were less resistant to drought stress, thus posing a threat to the stability and sustainability of the agroforestry system. Moreover, even in July and September with the high SWC in agroforestry, the partitioning of soil water among the three coexisting plants did not increase with increased ET loss. The three coexisting plants showed strong competition for soil water use, as indicated by the high values (0.69–0.97) of PS. Overall, the hydrological niche overlap of the coexisting plants in the agroforestry system resulted in low ecosystem stability. Density reduction or selection of suitable species is required to achieve sustainable vegetation restoration.

Variations in water sources used by winter wheat across distinct rainfall years in the North China Plain

Quantifying water availability in different soil layers to crop root water uptake (RWU) in different rainfall years helps deliver strategies to combat water scarcity and improve water productivity. To investigate the responses of RWU to soil water availability across distinct rainfall years, a field experiment of winter wheat was conducted in 2017–2018 (wet year), 2018–2019 (dry year), and 2019–2020 (ordinary year) in the North China Plain. Stable isotopes (δ18O and δD) in precipitation, irrigation water, xylem water, and soil water, as well as soil water content (SWC), were investigated during the three wheat growth seasons and were used to determine the soil water evaporation line (SWEL: the linear relationship between δD and δ18O in soil water), the line-conditioned excess (lc-excess: the deviation between δD and the local meteoric water line in the dual-isotope space) and the proportion of RWU from different soil layers with the MixSIAR model. The results indicated that there were significant differences in δ18O and δD in soil water and xylem water across different rainfall years. The slopes of SWEL and lc-excess of the 0–100 cm soil layer in the dry year were significantly smaller than those in the ordinary and wet years, which suggests that more substantial soil evaporation occurred in the dry year. The MixSIAR results revealed that the three-year wheat RWU exhibited different patterns, which were related to soil water availability. Wheat mainly utilized soil water from the 0–20 cm and 20–60 cm layers during the greening to filling stage under the dry year, while it mainly utilized the 0–20 cm soil layer under the wet and ordinary years. However, at the harvest stage, wheat under all precipitation years could transfer its main RWU zone to the 20–60 cm soil layer. There were significantly different proportions of RWU from 0 to 60 cm under different rainfall years, shown as 75.3 %, 67.5 %, and 69.5 % in the wet year, dry year, and ordinary year, respectively. These findings indicated that the appropriate irrigation wetting depth should be set as<60 cm for all kinds of rainfall years.

Investigating the impacts of biochar on water fluxes in a rice experiment in the dry corridor of Central America, Costa Rica

AbstractAmending soils with biochar, a pyrolyzed organic material, is an emerging practice to potentially increase plant available water and reduce the risks associated with climatic variability in traditionally‐rainfed tropical agricultural systems. To investigate the impacts of biochar amendment on soil water storage relative to non‐amended soils, we performed an upland rice field experiment in a tropical seasonally dry region of Costa Rica consisting of plots with two different biochar amendments and a control plot. Across all plots, we collected hydrometric and isotopic data (δ18O and δ2H of rain, mobile soil, ground and rice xylem water). We observed that the soil water retention curves for biochar treated soils shifted, indicating that rice plants had 2% to 7% more water available throughout the growing season relative to the control plots and thus could withstand dry spells up to seven extra days. Furthermore, the isotopic composition of plant water in biochar and control treatments were rather similar, indicating that rice plants in different treatments likely consumed similar water. Hence, we observed that biochar amendments can stabilize water supplies for the rice plants; however, still supplemental irrigation was required to facilitate plant growth during extended dry periods. Ultimately, our findings indicate, that biochar amendments can complement, but not necessarily replace, other water management strategies to help reduce the threat of rainfall variability to rainfed agriculture in tropical regions.