δ15N Of Organic Matter Research Articles

A nitrogen isotopic shift in fish otolith–bound organic matter during the Late Cretaceous

The nitrogen isotopes of the organic matter preserved in fossil fish otoliths (ear stones) are a promising tool for reconstructing past environmental changes. We analyzed the 15N/14N ratio (δ15N) of fossil otolith-bound organic matter in Late Cretaceous fish otoliths (of Eutawichthys maastrichtiensis, Eutawichthys zideki and Pterothrissus sp.) from three deposits along the US east coast, with two of Campanian (83.6 to 77.9 Ma) and one Maastrichtian (72.1 to 66 Ma) age. δ15N and N content were insensitive to cleaning protocol and the preservation state of otolith morphological features, and N content differences among taxa were consistent across deposits, pointing to a fossil-native origin for the organic matter. All three species showed an increase in otolith-bound organic matter δ15N of ~4‰ from Campanian to Maastrichtian. As to its cause, the similar change in distinct genera argues against changing trophic level, and modern field data argue against the different locations of the sedimentary deposits. Rather, the lower δ15N in the Campanian is best interpreted as an environmental signal at the regional scale or greater, and it may be a consequence of the warmer global climate. A similar decrease has been observed in foraminifera-bound δ15N during warm periods of the Cenozoic, reflecting decreased water column denitrification and thus contraction of the ocean's oxygen deficient zones (ODZs) under warm conditions. The same δ15N-climate correlation in Cretaceous otoliths raises the prospect of an ODZ-to-climate relationship that has been consistent over the last ~80 My, applying before and after the end-Cretaceous mass extinction and spanning changes in continental configuration.

Azoic sediments and benthic foraminifera: Environmental quality in a subtropical coastal lagoon in the gulf of California

Marine transitional environments play an important role in human sustainability. Around these ecosystems, coastal lagoons are subject to high anthropogenic pressure from population growth. The increased demand for goods and services is associated with the elevated discharge of untreated and treated wastewater into lagoon systems. The absence of benthic organisms in lagoon environments has been linked to extreme natural conditions and severe anthropogenic impact at both spatial and temporal scales. However, the mechanisms that lead to the presence of azoic sediments in lagoon environments have yet to be studied. This study aimed to determine the vertical variability of textural groups, geochemistry, and benthic foraminiferal fauna to understand how natural and anthropogenic components generate a vertical sediment sequence with low or absent benthic foraminifera in a subtropical coastal lagoon in the southwestern end of the Gulf of California. A 41 cm-long sediment core was collected from La Paz Lagoon at a 1-m depth. The core was sectioned every centimeter, and sediment subsamples were dried and homogenized for grain size, calcium carbonate, elemental and isotopic carbon and nitrogen analyses, and benthic foraminifera quantification. Muds with fine sands towards the core's base characterized the sedimentary sequence. Organic carbon and total nitrogen increased from the base (1.4% and 0.06%, respectively) to the core-top (CT, 3.0% and 0.14%, respectively), significant from the 27 cm interval. Calcium carbonate content was very low (<0.8%). The relationship of δ13C vs. C:N ratio indicated that sedimentary organic carbon was derived from the marine and sewage source mixture. The δ15N of organic matter increased by 3.7‰, starting from the 27 cm interval towards the CT. The nitrogen sewage input source was relatively more significant than nitrogen fixation. The few individuals (<18 ind. in 10 g) and genera (Ammonia and Elphidium), as well as the absence of foraminifera in 19 of 41 intervals in the core, indicated that environmental conditions were unfavorable, even for colonization of environmentally stress-tolerant genera. The frequency of azoic sediments was higher from the 25 cm interval to the CT vs. from the base to the 25 cm interval. Moreover, the AEI revealed severe to moderate hypoxia in the study area. The limited presence of benthic foraminifera and calcium carbonate preservation corroborated that the quality of the lagoon's environment has deteriorated along with population growth, which requires strategic programs to sustain this transitional ecosystem.

Eutrophication leads to food web enrichment and a lack of connectivity in a highly impacted urban lagoon

Nitrogen (N) loading can affect estuarine food webs through alteration of primary producers. In the Indian River Lagoon (IRL), Florida there has been long-term N enrichment, worsening phytoplankton blooms, large-scale macroalgal blooms, and catastrophic seagrass losses. To investigate how N enrichment affects higher trophic levels and food webs in the IRL, nutrient availability was compared to primary producer and faunal stable N (δ15N) isotope values. Seawater samples were collected in the IRL for dissolved nutrient, chlorophyll-a, and particulate organic matter δ15N analyses. Macrophytes and fauna were also collected for δ15N analyses. Throughout the IRL, N was elevated but was highest in the northern IRL and Banana River Lagoon. δ15N was enriched in these segments for most samples to levels characteristic of human-waste impacted estuaries. Variability in δ15N among lagoon segments suggests a low level of trophic connectivity. Decreasing N loading to the IRL and other eutrophic estuaries may help improve resiliency.

Environmental and microbial influence on chemistry and dolomite formation in an ancient lake, Green River Formation (Eocene), Uinta basin, Utah, U.S.A.

ABSTRACT Integrated stratigraphic, petrographic, and geochemical data allow interpretation of biogeochemical and mineralization processes in paleoenvironmental context of ancient lacustrine environments. These indicate that lake chemistry, microbial processes, and organic matter (OM) strongly influenced dolomite formation in near-surface environments throughout deposition of the Green River Formation (Eocene, Uinta basin, Utah). The lower Green River Formation consists of interbedded fluvio-deltaic siliciclastics, paleosols, carbonate mud, coated-grain carbonates, mollusk and ostracod limestones, and microbialites all landward of profundal OM-bearing illitic mudrocks. Calcite, dolomite, Fe-dolomite, and authigenic feldspars are common. Carbonate δ18O and δ13C are covariant, and positive excursions of carbonate δ13C (up to 6.9‰ VPDB) and organic-matter δ15N (up to 13.9‰ V-AIR) occur in profundal OM-bearing mudrocks. The upper Green River Formation consists mainly of laminated OM-lean and OM-rich dolomitic muds (i.e., “oil-shales”). Zoned dolomite crystals with Mg-calcite centers and Fe-dolomite rims are widespread in addition to authigenic feldspars and Na-carbonates. Carbonate δ13C-enrichment (up to 15.8‰ VPDB), and organic-matter δ15N-enrichment (up to 18.4‰ V-AIR) occur in these OM-rich dolomite muds. Organic-matter δ13C is relatively invariable (mean = –29.3‰ VPDB) and does not covary with carbonate δ13C. Trends in mineralogy, organic-matter abundance, and stable isotopes result from changing hydrologic systems, paleoclimate, lake chemistry and microbial processes coincident with the Early Eocene Climate Optimum. The lower Green River Formation paleo-lake was smaller in area and volume, heavily influenced by meteoric fluvial input, variably oxygenated, and ranged from neutral and fresh to alkaline and saline. Especially in littoral environments with abundant microbialites, dolomite formed through recrystallization of precursor carbonate involving both replacement of precursor carbonate and direct precipitation as cements and overgrowths. The upper Green River Formation paleo-lake was more expansive with widespread low-oxygen, nutrient-rich, and alkaline saline environments with increased planktic organic-matter productivity. Microbial decay of organic matter in low-oxygen environments produced alkaline lake waters through methanogenesis, possible denitrification, and bacterial sulfate reduction to a limited degree. This favored precipitation of widespread dolomite, as well as Na-carbonates, authigenic feldspars, and analcime from lake water and phreatic pore water. Extracellular polymeric substances (EPS) excreted by microbial communities provided favorable nucleation sites for Mg-carbonate, allowing kinetic barriers of low-temperature dolomite formation to be overcome. Cycling of pH due to turnover of organic matter and associated microbial processes potentially bolstered EPS generation and abiotic environmental conditions favorable to dolomite precipitation. It is likely that metastable precursor carbonate was recrystallized to ordered dolomite, but it is possible that direct precipitation occurred. Fe-dolomite overgrowths precipitated after dolomite where microbial Fe reduction occurred in stagnant, oxygen-depleted, alkaline pore waters.

Variability of the oxygen minimum zone associated with primary productivity and hydrographic conditions in the Eastern North Pacific during the last 1200 years

The expansion of the oxygen minimum zone (OMZ) associated with global warming has generated interest in its variability during the last two millennia. Several oceanographic mechanisms, as advection of dissolved oxygen and depletion of dissolved oxygen by oxidation of exported marine productivity, could explain the variability of δ15N in organic matter as a denitrification indicator of the water column in the Pacific Ocean. Our objective was to infer local or remote forcing mechanisms that lead to the strengthening or weakening of the OMZ in the Eastern Tropical North Pacific. A 42 cm sediment core was recovered at 680 m of depth in the Magdalena margin and sectioned every 1 cm for carbon and nitrogen stable isotope analysis. During the periods 1260–1420 CE and 1670–1800 CE, the desynchronization of δ15N between the Pescadero and Magdalena margins with respect to the Santa Barbara Basin confirms a decrease in denitrification in the Mexican Pacific. During the Little Ice Age (LIA: 1450 to 1800 CE), the δ15N indicated a reduction in denitrification in the Magdalena margin and the Santa Barbara Basin, but not in the Pescadero margin. This suggests an intense advection of dissolved oxygen from tropical waters during the LIA. In the Pescadero margin, denitrification conditions persisted over the same period, while exported productivity maintained a high oxygen demand.

Fish isotopic niches associated with environmental indicators and human disturbance along a disturbed large subtropical river in China

Stable isotopes are increasingly used to detect and understand the impacts of environmental changes on riverine ecological properties. The δ13C and δ15N signatures of fish with different feeding habits were measured in a large subtropical river to evaluate how fish isotopic niches respond to environmental gradients and human disturbance. From basal resources to fish consumers, the high values of epilithic periphyton (biofilm) δ13C and suspended particulate organic matter δ15N concurrently determined the niche ranges and space (e.g., convex hull area) of fish communities. Along a longitudinal gradient (except in the industrial zone), the number of fish trophic guilds identified by Bayesian ellipses continuously increased; meanwhile, higher trophic diversity and less redundancy were observed near the lower reaches and estuary. Variance inflation factors were estimated to detect the multicollinearity of 40 environmental variables, 14 of which were selected as indicators. Relative importance (RI) analysis was used to evaluate the explanatory power of these indicators for the spatial variation in isotopic niche metrics; the results showed that riffle habitat area, water nitrate concentration, gravel-cobble substrate, and riparian buffer width were the 4 key environmental indicators (average RI > 12%) that determined the longitudinal pattern of fish isotopic niches. These findings suggested that community-level δ13C signatures are more responsive to changes in habitats (e.g., riffle) and substrates (e.g., gravel-cobble) supporting the productivity of autochthonous diatoms while δ15N signatures respond to water quality altered by nitrogen pollution from manure-fertilized farming and poultry livestock effluent. Furthermore, δ15N may be more robust and interpretable than δ13C as an isotopic indicator of ecosystem change in rivers exposed to multiple or complex anthropogenic stressors.

Tidal Freshwater Zones as Hotspots for Biogeochemical Cycling: Sediment Organic Matter Decomposition in the Lower Reaches of Two South Texas Rivers

While organic and inorganic nutrient inputs from land are recognized as a major driver of primary production in estuaries, remarkably little is known about how processes within the tidal freshwater zones (TFZs) of rivers modify these inputs. This study quantifies organic matter (OM) decomposition rates in surface sediment layers in the lower reaches of two south Texas river channels and identifies key parameters that influence sediment decomposition rates. Sediment cores were collected from non-tidal and tidal freshwater sites in the Mission and Aransas rivers during two summers (June 2015 and June 2016) and two winters (February 2016, January 2017). We measured oxygen consumption rates, organic carbon and nitrogen content, stable isotope ratios (δ13C and δ15N of OM), and sediment porosity. O2 consumption rates in TFZ sediments were 385 ± 88 μmol O2 m−2 h−1 (summer) and 349 ± 87 μmol O2 m−2 h−1 (winter) in the Aransas River and 767 ± 153 μmol O2 m−2 h−1 (summer) and 691 ± 95 μmol O2 m−2 h−1 (winter) in the Mission River. These rates in TFZs were similar to rates in estuaries and higher than rates at non-tidal riverine sites. Rates of sediment O2 consumption were primarily controlled by OM content and temperature. Sediment OM was dominated by algal biomass from in situ production in both TFZs. We hypothesize that algal production and sinking within TFZs is a major pathway for translocation of watershed-derived nutrients from the water column to the sediments within TFZs. Further work is needed to quantify linkages between decomposition, nutrient remineralization, and potential removal through processes such as denitrification.

Recent onset of eutrophication in Lake Izabal, the largest water body in Guatemala

Recent human population growth has contributed to the degradation of environmental resources in Guatemala. Since 1900, the population of the country grew from 17 million inhabitants. The watershed of Lake Izabal, eastern Guatemala, also experienced rapid population growth, and with it, development of intensive agriculture in areas adjacent to the lake. These recent demographic and land-use changes altered anthropogenic nutrient inputs to the water body, through delivery of raw sewage, and agricultural practices that include excessive use of fertilizers. Currently, Lake Izabal is marked by frequent algal blooms and associated fish-kill events. It also hosts a number of invasive, and in some cases, exotic plant and animal species. We used data from a sediment core collected in the western end of the lake to link past anthropogenic activities in the watershed to the deterioration of water quality and shifts in aquatic ecosystem functioning. We measured total organic carbon (TOC), total nitrogen (TN), and δ13C and δ15N of organic matter in a 137Cs- and 14C-dated sediment core from the Polochic Delta in Lake Izabal to infer past limnological changes. The core chronology indicates that 505 cm of sediment accumulated in only the last 370 years (~ 1.4 cm a−1). TOC and TN concentrations increased since about 1950, a time during which the TOC:TN ratio decreased, indicating a relatively greater contribution of phytoplankton to the sediment organic matter. Stratigraphic shifts in δ13C and δ15N indicate recent increasing inputs of raw sewage and fertilizer to the lake during the same time period. Lake Izabal, like many other water bodies in Guatemala, is starting to undergo cultural eutrophication, which probably accounts for the frequent algal blooms, proliferation of invasive species (e.g. Hydrilla verticillata), and decline in fish abundance.

The environmental impact of a pre-Columbian city based on geochemical insights from lake sediment cores recovered near Cahokia

AbstractCahokia is the largest documented urban settlement in the pre-Columbian United States. Archaeological evidence suggests that the city, located near what is now East St. Louis, Illinois, began to rapidly expand starting around AD 1050. At its height, Cahokia extended across 1000 ha and included large plazas, timber palisade walls, and hundreds of monumental earthen mounds. Following several centuries of occupation, the city experienced a period of gradual abandonment from about AD 1200 to 1400. Here, we present geochemical data from a 1500-year-old sediment core from nearby Horseshoe Lake that records watershed impacts associated with the growth and decline of Cahokia. Sedimentary analysis shows a distinctive 24-cm-thick, gray, fine-grained layer formed between AD 1150 and 1220 and characterized by low carbonate δ13C, elevated sorbed metal concentrations, and higher organic matter δ15N. The deposition of this layer is contemporaneous with archaeological evidence of increased agricultural activity, earthen mound construction, and higher populations surrounding the lake. We hypothesize that these human impacts increased soil erosion, producing new sediment sources from deeper soil horizons, and shifted dissolved transport to the lake, producing lower carbonate δ13C values, higher concentrations of lead, copper, potassium, and aluminum, and increased δ15N, likely due to contributions of enriched nitrogen from sewage.

The isotope effect of nitrate assimilation in the Antarctic Zone: Improved estimates and paleoceanographic implications

Both the nitrogen (N) isotopic composition (δ15N) of the nitrate source and the magnitude of isotope discrimination associated with nitrate assimilation are required to estimate the degree of past nitrate consumption from the δ15N of organic matter in Southern Ocean sediments (e.g., preserved within diatom microfossils). It has been suggested that the amplitude of isotope discrimination (i.e. the isotope effect) correlates with mixed layer depth, driven by a physiological response of phytoplankton to light availability, which introduces complexity to the interpretation of sedimentary records. However, most of the isotope effect estimates that underpin this hypothesis derive from acid-preserved water samples, from which nitrite would have been volatilized and lost during storage. Nitrite δ15N in Antarctic Zone surface waters is extremely low (−61 ± 20‰), consistent with the expression of an equilibrium isotope effect associated with nitrate–nitrite interconversion. Its loss from the combined nitrate + nitrite pool would act to raise the δ15N of nitrate, potentially yielding overestimation of the isotope effect. Here, we revisit the nitrate assimilation isotope effect in the Antarctic Zone with measurements of the δ15N and concentration of nitrate with and without nitrite, using frozen sea water samples from 5 different cruises that collectively cover all sectors of the Southern Ocean. The N isotope effect estimated using nitrate + nitrite δ15N is relatively constant (5.5 ± 0.6‰) across the Antarctic Zone, shows no relationship with mixed layer depth, and is in agreement with sediment trap δ15N measurements. Estimates of the N isotope effect derived from nitrate-only δ15N are higher and more variable (7.9 ± 1.5‰), consistent with an artifact from nitrate-nitrite isotope exchange. In the case of the Southern Ocean, we conclude that the δ15N of nitrate + nitrite better reflects the isotope effect of nitrate assimilation. The stability of this isotope effect across the Antarctic Zone simplifies the effort to reconstruct the past degree of nitrate consumption.

Control of the Nitrogen Isotope Composition of the Fungal Biomass: Evidence of Microbial Nitrogen Use Efficiency.

Changes in 15N/14N in the soil microbial biomass during nitrogen (N) mineralization have been hypothesized to influence 15N/14N in soil organic matter among ecosystem sites. However, a direct experimental test of this mechanism has not yet been performed. To evaluate the potential control of microbial N mineralization on the natural N isotope composition, we cultured fungi (Aspergillus oryzae) in five types of media of varying C:N ratios of 5, 10, 30, 50, and 100 for 4 d, and tracked changes in δ15N in the microbial biomass, NH4+, and dissolved organic N (DON: glycine) over the course of the experiment. High rates of NH4+ excretion from A. oryzae were accompanied by an increase in δ15N in the microbial biomass in low C:N media (i.e., C/N<30). In contrast, NH4+ was strongly retained in higher C/N treatments with only minor (i.e., <1 ‰) changes being detected in δ15N in the microbial biomass. Differences in δ15N in the microbial biomass were attributed to the loss of low-δ15N NH4+ in low, but not high C/N substrates. We also detected a negative linear correlation between microbial nitrogen use efficiency (NUE) and Δ15N (δ15N-biomass–δ15N-glycine). These results suggest an isotope effect during NH4+ excretion in relatively N-repleted environments in which microbial NUE is low, which may explain the vertical patterns of organic matter δ15N in soil profiles.

Modelling Palaeoecological Time Series Using Generalised Additive Models

In the absence of annual laminations, time series generated from lake sediments or other similar stratigraphic sequences are irregularly spaced in time, which complicates formal analysis using classical statistical time series models. In lieu, statistical analyses of trends in palaeoenvironmental time series, if done at all, have typically used simpler linear regressions or (non-) parametric correlations with little regard for the violation of assumptions that almost surely occurs due to temporal dependencies in the data or that correlations do not provide estimates of the magnitude of change, just whether or not there is a linear or monotonic trend. Alternative approaches have used LOESS-estimated trends to justify data interpretations or test hypotheses as to the causal factors without considering the inherent subjectivity of the choice of parameters used to achieve the LOESS fit (e.g. span width, degree of polynomial). Generalized additive models (GAMs) are statistical models that can be used to estimate trends as smooth functions of time. Unlike LOESS, GAMs use automatic smoothness selection methods to objectively determine the complexity of the fitted trend, and as formal statistical models, GAMs, allow for potentially complex, non-linear trends, a proper accounting of model uncertainty, and the identification of periods of significant temporal change. Here, I present a consistent and modern approach to the estimation of trends in palaeoenvironmental time series using GAMs, illustrating features of the methodology with two example time series of contrasting complexity; a 150-year bulk organic matter δ15N time series from Small Water, UK, and a 3000-year alkenone record from Braya-So, Greenland. I discuss the underlying mechanics of GAMs that allow them to learn the shape of the trend from the data themselves and how simultaneous confidence intervals and the first derivatives of the trend are used to properly account for model uncertainty and identify periods of change. It is hoped that by using GAMs greater attention is paid to the statistical estimation of trends in palaeoenvironmental time series leading to more a robust and reproducible palaeoscience.

Evidence of intense suboxia and anoxia in a tropical bay of the Gulf of California

The expansion of the upper boundary of the oxygen minimum zone (OMZ) has been associated with the low solubility of oxygen in the surface ocean due to global warming in tropical regions. However, sedimentary records of δ15N of sinking particulate organic matter and sediments suggest that oxygen solubility is controlled by oceanographic forcing in the tropical equatorial region. To determine the intensification of the OMZ within its upper border, δ15N analysis of the organic matter in superficial sediments of La Paz Bay was carried out. In the present work, 115 samples of superficial sediment were collected in the interval of 2–407 m depth in La Paz Bay. The surface sediments were analysed for the nitrogen isotopic composition of their organic matter. Hypoxia conditions (<60 μM) at 100 m to depth suggest an intense denitrification of the water column that it was reflected in the sedimentary δ15N. The δ15N of the organic matter is 1.5‰ greater in the bay than in the mouth of the Gulf of California, indicating greater denitrification towards the interior of the bay with respect to the gulf mouth. The intense suboxia/anoxia conditions have been maintained due to a well-mixed water column that promotes exported productivity in La Paz Bay.

Nitrate Isotope Distribution in the Subarctic and Subtropical North Pacific

AbstractNitrogen and oxygen isotopic compositions of nitrate (δ15NNitrate and δ18ONitrate) were determined along 47°N in the subarctic North Pacific and 149°E in the western North Pacific. In the western subarctic gyre, known as a high‐nutrient, low‐chlorophyll region, the δ15NNitrate and the differences between δ15NNitrate and δ18ONitrate (herein Δ(15–18)) in the intermediate water were significantly lower than in the surrounding area. The 15N‐depleted nitrate was generated by nitrified nitrate from the remineralization of organic matter produced by the partial consumption of the surface nitrate pool. Between the western subarctic gyre and the Alaskan gyre, there was a 0.2‰ increase in δ15NNitrate and a 0.3‰ increase in Δ(15–18) in the intermediate water associated with a 7.2‐μM decrease in the nitrate concentration at the surface. Assuming Rayleigh distillation kinetics, the increase in utilization of the surface nitrate pool between the western subarctic gyre and the Alaskan gyre (from 29% to 85%) corresponds to a change in δ15N of organic matter from 2.6‰ to 4.9‰. The 15N enrichment of intermediate nitrate toward the east resulted from the increase in utilization. Moreover, the 15N‐depleted nitrate in the mixed‐layer water of the western subtropical gyre was affected by N2 fixation in the Kuroshio water, whereas the 15N‐enriched nitrate in the intermediate water at the western margin of North America was affected by water column denitrification. The δ15N sediment record in the western subarctic North Pacific likely reflects past changes in the high‐nutrient, low‐chlorophyll region but may also be controlled by water column denitrification and N2 fixation.

Ground-truthing the planktic foraminifer-bound nitrogen isotope paleo-proxy in the Sargasso Sea

We report the nitrogen (N) isotope ratios (δ15N) of planktic foraminifera collected from upper-ocean net tows (surface to 200 m), moored sediment traps, and core-top sediments at the Bermuda Time-series Site in the northern Sargasso Sea between 2009 and 2013. Consistent with previous measurements from low-latitude core-top sediments, the annually-averaged δ15N of organic matter bound within the shells of euphotic zone-dwelling, dinoflagellate symbiont-bearing foraminifera collected in net tows (2.3‰ on average) approximates that of shallow thermocline (∼200 m) nitrate (2.6‰), the dominant source of new N to Sargasso Sea surface waters. Deeper-dwelling foraminifer species without dinoflagellate symbionts tend to have a higher δ15N (3.6‰ on average). We observe no systematic difference between the bulk tissue and shell-bound δ15N in net tow-collected foraminifera. A decline in shell N content is observed from net tows (6.8 nmol/mg) to sediment traps (5.4 nmol/mg) and surface sediment (3.0 nmol/mg). On average, shell-bound δ15N rises from net tows (3.1‰) to sediment traps (3.7‰) but does not change further upon incorporation into the sediments (3.7‰). Together, these observations are consistent with preferential loss of shells or shell portions with lower δ15N and higher N content during sinking through the upper 500 m, followed by a non-isotope fractionating decrease in N content between sinking and burial. Time-series data from sediment traps (and to a lesser extent, surface net tows) exhibit seasonal δ15N variations, with a minimum in early spring, a maximum in late spring and a decline from summer to fall. These variations appear to arise from seasonal changes in the δ15N of total upper-ocean biomass, which are, in turn, driven by early springtime nitrate supply, subsequent nitrate drawdown, and an increase in the relative importance of ammonium recycling into the late summer and early fall. The δ15N connection between total upper ocean biomass and foraminifera indicates that foraminifer-bound δ15N records the δ15N of the annual nitrate supply in oligotrophic (e.g., subtropical) environments but will also be sensitive to the degree of nitrate consumption in high-nutrient regions and possibly to changes in upper-ocean ammonium recycling under some conditions.

Testing the validity of stable isotope analyses of dental calculus as a proxy in paleodietary studies

Stable isotopic analyses (δ13C, δ15N) of dental calculus have been suggested as a proxy for the study of diet of ancient populations but questions about their validity have been raised. Here we test this question, introducing significant improvements in the analysis of δ13C and comparing our results for δ13C and δ15N of calculus with corresponding analyses of associated well-preserved bone which are widely believed to provide reliable paleodiet values. The content of organic material in calculus is decreased by ∼75% compared with modern calculus, resulting in diagenetic changes to δ13C and δ15N of organic matter. Neither δ13C nor δ15N analyses of the organic component of calculus provide accurate estimates of paleodiet. Although δ15N values of dental calculus are correlated with δ15N values of bone collagen from the same individual, it is clear that they have been greatly affected by diagenesis, as shown by a correlation between C/N ratio and δ15N. The inorganic (mineral-bound) carbon component of calculus, analyzed separately from the organic component, gave δ13C values slightly offset from δ13C values of CO3 in bone mineral. Thus it alone appears to have potential as a dietary proxy.

An isotopic model for the origin of autochthonous organic matter contained in the bottom sediments of a reservoir

Geochemical analysis of surface sediment samples collected in 2005 and 2006 was used to evaluate the potential sources of the organic matter present in sediments of southeast Poland's Solina Reservoir. Statistical analysis of sediment variables (carbon to nitrogen ratio, and the carbon 13 and nitrogen 15 isotope ratios) determined for the organic fraction indicated significant spatial variability with respect to sources of organic matter. A binary mixing model was developed from literature sources to predict the relative contributions of allochthonous and autochthonous production to sediment organic matter. Autochthonous production was shown to account for 60–75% of bulk sedimentation in the lacustrine parts of the reservoir, near the dam. In contrast, autochthonous production accounted for only 25% of sedimentation in the riverine zone receiving stream inputs. Statistical analysis identified the δ15N of organic matter as the best predictor of the source of organic matter. Multiple regression analysis indicated that two water-quality variables (nitrate and dissolved silica) were significantly related to the δ15N signature of organic matter. This led to a conclusion that limnetic nitrate and dissolved silica concentrations were regulating organic matter production in the Solina Reservoir.

A 15.4-ka paleoclimate record inferred from δ13C and δ15N of organic matter in sediments from the sub-alpine Daping Swamp, western Nanling Mountains, South China

We inferred past climate conditions from the δ13C and δ15N of organic matter (OM) in a sediment core (DP-2011-02) from the sub-alpine Daping Swamp, in the western Nanling Mountains, South China. In the study region, a 1000-m increase in altitude results in a ~0.75‰ decrease in δ13C and a ~2.2‰ increase in δ15N. Organic carbon stable isotope (δ13C) values of the dominant modern vegetation species, surface soils, and the core samples taken in the swamp exhibit a strong terrestrial C3 plant signature. Comprehensive analysis of the core indicates both terrestrial and aquatic sources contribute to the OM in sediment. Temperature and precipitation are most likely the critical factors that influence δ13C: warm and wet conditions favor lower δ13C, whereas a dry and cool climate leads to higher δ13C values. Higher δ15N values may result from greater water depth and increased primary productivity, promoted by large inputs of dissolved inorganic nitrogen, induced by high surface runoff. Lower δ15N values are associated with lower lake stage and reduced productivity, under drier conditions. Therefore, stratigraphic shifts in these stable isotopes were used to infer past regional climate. Measures of δ13C and δ15N in deglacial deposits, in combination with total organic carbon (TOC) and nitrogen (TN) concentrations, the TOC/TN ratio, coarse silt and sand fractions, dry bulk density and low-frequency mass magnetic susceptibility, reveal two dry and cold events at 15,400–14,500 and 13,000–11,000 cal a BP, which correspond to Heinrich event 1 and the Younger Dryas, respectively. A pronounced warm and wet period that occurred between those dry episodes, from 14,500 to 13,000 cal a BP, corresponds to the Bolling–Allerod. The δ13C and δ15N data, however, do not reflect a warm and wet early Holocene. The Holocene optimum occurred between ~8000 and 6000 cal a BP, which is different from inferences from the nearby Dongge cave stalagmite δ18O record, but consistent with our previous results. This study contributes to our understanding of climate-related influences on δ13C and δ15N in OM of lake sediments in South China.

Processes affecting the spatial distribution of seagrass meadow sedimentary material on Yao Yai Island, Thailand

Many islands throughout SE Asia are experiencing rapid development and land-cover conversion that potentially threaten sensitive coastal ecosystems, such as seagrasses, through increased loading of sediment and nutrients originating from disturbed catchments draining to the sea. To evaluate this threat for one such island in Southern Thailand (Yao Yai), we perform sediment source tracing via end-member mixing analysis using stable isotopes δ13C and δ15N in organic matter to explore sediment loading in a seagrass meadow. The analysis indicates that sedimentary material in the meadow originates mostly from ocean-associated sources (∼62% from seagrass detritus, seston, and ocean sediments). Terrestrial material comprises ∼19% of the organic material found in the seagrass meadow, with another 20% originating from an adjacent mangrove forest. Approximately one-fourth of the seagrass meadow material (24%) is detritus that has been (re)deposited internally. The high contribution of terrestrial-derived organic matter deposited near the river mouth demonstrates that substantial quantities of sediment are being transferred from upslope erosion sources into the seagrass meadow. However, only a small amount of this material is deposited throughout the entire bay because much of the terrestrial- and mangrove-derived sediment is transferred to the open ocean via channels that are periodically dredged to allow boat access to two small inland harbours. This positive affect of dredging has not received very much attention in existing literature. River water flowing to the channels during falling tide delivers sediment to these efficient pathways, where much of it bypasses the seagrass meadow at periods of time when sediment deposition would normally be the greatest. There is growing concern that ongoing land-cover changes and planned urbanization related to tourism and agriculture on the island may boost sediment/nutrients above a critical threshold, beyond that revealed in our baseline survey. Our tracer-based sediment source approach did not corroborate our observations of substantial erosion and land degradation in the upper catchment—but this could be a result of sediment flushing through the dredged channels. We encourage others to combine such methods with sediment budgeting approaches to triangulate results for consistency. Finally, from an ecological perspective, the high presence of seagrass detritus we found in bay sediments suggests seagrass is potentially a key source of nutrients for the meadow itself, as well as other connected ecosystems.

Carbon and nitrogen isotopes analysis and sources of organic matter in surface sediments from the Sanggou Bay and its adjacent areas, China

Naturally existing stable carbon and nitrogen isotopes are important in the study of sedimentary organic matter sources. To identify the sources of sedimentary organic matter in Sanggou Bay and its adjacent areas, which is characterized by high-density shellfish and seaweed aquaculture, the grain size, organic carbon (OC), total nitrogen (TN), carbon and nitrogen isotopic composition (δ13C and δ15N) of organic matter in the surface sediment were determined. The results showed that, in August, sedimentary OC and TN ranged from 0.17% to 0.76% and 0.04% to 0.14%, respectively. In November, OC and TN ranged from 0.23% to 0.87% and 0.05% to 0.14%, respectively. There was a significant positive correlation between OC and TN (R=0.98, P<0.0001), indicating that OC and TN were homologous. In August, the δ13C and δ15N of organic matter varied from −23.06‰ to −21.59‰ and 5.10‰ to 6.31‰, respectively. In November, δ13C and δ15N ranged from −22.87‰ to −21.34‰ and 5.13‰ to 7.31‰, respectively. This study found that the major sources of sedimentary organic matter were marine shellfish biodeposition, seaweed farming, and soil organic matter. Using a three-end-member mixed model, we estimated that the dominant source of sedimentary organic matter was shellfish biodeposition, with an average contribution rate of 65.53% in August and 43.00% in November. Thus, shellfish farming had a significant influence on the coastal carbon cycle.