Cytokine Status In Patients Research Articles

ЭФФЕКТИВНОСТЬ ИММУНОТЕРАПИИ У БОЛЬНЫХ АЛЛЕРГИЧЕСКИМ ПЕРСИСТИРУЮЩИМ РИНИТОМ

The dynamics of parameters of cytokine status in patients with allergic rhinitis in view of efficiency of allergen-specific immunotherapy (ASIT) was studied in this work. 62 subjects with moderate persistent rhinitis at the age of 18-40 years old were included in the study. For specific diagnosis and treatment there we used standard allergens. ASIT was performed by the classical scheme. The concentrations of IL-4, IL-5, IL-6, IL-8 in the blood serum were measured by the enzyme immunoassay. A good or excellent effect of treatment with ASIT was achieved in 62.9% of cases, and satisfactory one in 37.1% of cases. The analysis of the dynamics of IL-4, IL-5, IL-6, IL-8 concentrations showed that the efficiency of ASIT in patients with allergic rhinitis depends on the ability of the immune system to activation by low doses of the allergen, quite fast suppression of proinflammatory cytokines induced by them, and active functioning of the system of anti-inflammatory cytokines.

Цитокиновый статус пациентов с хроническим вирусным гепатитом В с дельта-агентом на различных стадиях фиброза.

Цитокиновый статус пациентов с хроническим вирусным гепатитом В с дельта-агентом на различных стадиях фиброза.

ХАРАКТЕРИСТИКА ИММУННОГО И ЦИТОКИНОВОГО СТАТУСА БОЛЬНЫХ ИНТЕРСТИЦИАЛЬНЫМИ БОЛЕЗНЯМИ ЛЕГКИХ

ХАРАКТЕРИСТИКА ИММУННОГО И ЦИТОКИНОВОГО СТАТУСА БОЛЬНЫХ ИНТЕРСТИЦИАЛЬНЫМИ БОЛЕЗНЯМИ ЛЕГКИХ

FEATURES OF CYTOKINE STATUS IN PATIENTS WITH CHRONIC EBV-INFECTIONS

Infections caused by EBV are the most common and occupy an important place in the structure of herpes aetiology diseases. The purpose of this work was to study the characteristics of the cytokine status in patients with chronic EBV infection, depending on the level of viral replication. We examined 78 patients with chronic EBV infection, the main clinical manifestations of which were various immunopathological and immunodeficiency states: Group I – with low, Group II – with medium, Group III - with a high degree of viral replication. The Tiff method was used using the Vector-Best reagent kits (Novosibirsk, Russia) to study the cytokine profile in the serum of patients with EBV infection. The determination of alpha and gamma fractions of serum interferon was carried out using the ELISA method by means of the ProCon IF2 plus reagent kit manufactured by Protein Contour LLC (St. Petersburg, Russia). As a result of a study of the cytokine status in patients with chronic EBV infection, it was found that in all three groups there was a significant increase in both pro-inflammatory (IL-1β, IL-6, TNF-α) and antiinflammatory cytokines (IL-10, IL 4, TGFβ1). However, anti-inflammatory cytokinemia was more compensated in group I patients compared with patients in groups II and III. A decrease in IFN-α and IFN-γ was detected in all patients with chronic EBV infection while studying the interferon status. A correlation was found between the level of viral replication and a decrease in the level of IFN-α and IFN-γ. The identified features of the cytokine status in patients with chronic EBV infection can be used to optimize therapy and help develop a differentiated approach to the immunocorrection of these patients, depending on the level of viral replication.

BALANCE OF Thl/Th2/Th9/Thl7/Th22 CYTOKINES IN POST-OPERATION PERIOD IN PATIENTS WITH MALIGNANT TUMOR OF LIVER

Evaluate cytokine status in patients with malignant liver cells after surgery. 33 patients aged 35 to 76 years were included into the study. Blood was obtained before the operation and in the post-operation period: after 6 and 24 hours and at day 7 Cytokine profile (IL-Ib, IL-2, TNF-α, IFN-γ, IL-12p70, IL-4, IL-5,IL-6, IL-10, IL-13, IL-9, I-17a, IL-22) was evaluated using Multiplex- 13 system (Bender MedSystems, Austria). In patients levels of all the studied cytokines (Thl/Th2/Th9/Thl7/Th22) were already increased before the operations, that gives evidence of the presence of an inflammatory proc- ess connected with activation of immune system effectors. Disbalance of cytokine system helper cells resulting in functional and organic alterations through induction of the "cytokine storm" may aggravate the state of these patients. Further studies on the correction of cytokine system in these patients are thus needed.

Cytokine status of patients with chronic obstructive pulmonary disease and type 2 diabetes mellitus

The review article is devoted to an actual problem of modern medicine - combined pathology of chronic obstructive pulmonary disease and type 2 diabetes mellitus. These 2 diseases take the first place in the structure of people’s mortality. The rate of chronic obstructive pulmonary disease and type 2 diabetes mellitus increases annually along with life length and according to different data reaches 35.8%. In the article scientists’ modern perception and known facts from scientific literature are presented. Special attention is given to cytokine status in chronic obstructive pulmonary disease and type 2 diabetes mellitus. Cytokines classification based on mechanism of action and their role in pathogenesis is presented. Systemic inflammation characteristic for both diseases presents as the increase of pro-inflammatory cytokines level in the blood and decrease of anti-inflammatory cytokines. Determining cytokines in patients with chronic obstructive pulmonary disease and type 2 diabetes mellitus has prognostic value. Mutual negative influence of these two diseases is directly associated with the increase of pro-inflammatory cytokines which are considered the reason for insulin resistance and type 2 diabetes mellitus. Despite the attempts to study cytokine status in patients with chronic obstructive pulmonary disease and type 2 diabetes mellitus the issues of treatment of combined pathology, diagnostic concentration of pro-inflammatory and anti-inflammatory cytokines, their role in pathogenesis and clinical presentation are still an open question. Certainly it is necessary to continue studying the cytokine status in patients with chronic obstructive pulmonary disease and type 2 diabetes mellitus to answer those questions.

Цитокиновый статус у больных диабетической кардиомиопатией

Цитокиновый статус у больных диабетической кардиомиопатией

Levels of cytokines in drug hypersensitivity.

IntroductionMultiple drug intolerance is a serious complication of drug therapy and is an issue of allergology. The aim of the study was the investigation of cytokine status in patients with drug hypersensitivity and multiple drug hypersensitivity.Material and methods19 patients with multiple drug hypersensitivity, 34 patients with hypersensitivity to one drug, and 35 non-allergic subjects were involved. Only women were included in the study. A multiplex assay of 27 cytokines and chemokines was performed using xMap technology (Human Cytokine Panel I by Bio-Rad).ResultsWomen with drug allergy revealed increased IL-2 levels (p < 0.05). In the case of the study of cytokine status in patients with multiple drug hypersensitivity, the new data revealed the prevalence of pro-inflammatory cytokine status with the participation of cytokines IL-17, IL-9, TNF-α, IP-10, and MIP-1.ConclusionsVarious immune response arms Th2, Th17, as well as macrophages were the determining factors in the cytokine balance that was found in patients with multiple drug hypersensitivity.

Особенности цитокинового профиля у больных псориатическим артритом

Особенности цитокинового профиля у больных псориатическим артритом

РОЛЬ МОНИТОРИНГА ЦИТОКИНОВОГО СТАТУСА У ПАЦИЕНТОВ С ЦЕРЕБРОФАЦИАЛЬНЫМИ ПОВРЕЖДЕНИЯМИ ДЛЯ ПРОГНОЗИРОВАНИЯ ОСЛОЖНЕННОГО ТЕЧЕНИЯ

According to different authors anatomical contiguity of viscerocranium and neurocranium determines the incidence of associated injuries, which is 6,3‑90 %. When there are concomitant injuries, the imbalance developes in the regulation of pro- and anti-inflammatory parts of the immune system, leading to pathogenic microorganisms activation and the development of inflammatory complications. This study contains a monitoring cytokine status in patients with cerebro-facial injuries. 60 patients (56 men and 8 women) at the ages from 20 to 50 with fractures of facial bones in combination with craniocerebral damages except for severe brain contusion are included into the study. The patients were studied for IL-6, IL-8, IL-10 and TNF in serum in the clinical laboratory of MAU «CGKB»s» (Central Clinical City Hospital № 23) by ELISA method on the 1st, 3rd, 7th and 10th day after injury. A significant increase in the concentration of proinflammatory cytokines IL-6, IL-8 (over 10 pg / ml) on the 1st day after trauma enhances the adequate immune response to the mechanical injury. The development of an imbalance in the immune response was observed in 9 % of cases and expressed in high concentrations of IL-10 (more than 31 pg / ml) and TNF (more than 6 pg / ml) with the clinical development of infectious complications (delayed union, osteomyelitis) in all cases.

Comparative characteristics of immune answers indicators depending on the replicative activity and genotype of hepatitis c virus

Comparative characteristics of immune answers indicators depending on the replicative activity and genotype of hepatitis c virus

Morphofunctional state of neutrophils and cytokine status in patients with ovarian cancer

The purpose of the study was to evaluate the morphofunctional state of polymorphonuclear neutrophils and cytokine levels in the lysate of blood neutrophils of ovarian cancer patients. The absolute and the relative number of peripheral blood polymorphonuclear neutrophils in 120 primary ovarian cancer patients (III-IV clinical stage FIGO) and 80 healthy women were determined. The expression of CD11a, 11b, 15 and CD95 in polymorphonuclear neutrophils was assessed by fluorescent microscopy. Cytochemically the myeloperoxidase activity, the content of cationic proteins, the activity in the spontaneous NBT-test and phagocytic activity in polymorphonuclear neutrophils was determined. The amounts of cytokines TNF-α, IL-1β, 1Ra, 6, 18, and G-CSF in serum were measured by the enzyme immunoassay method. Rigidity and topology of native polymorphonuclear neutrophils were assessed by scanning probe microscopy. To detect the differences between the data obtained in the evaluation of ovarian cancer patients and healthy people, a non-parametric U-Mann-Whitney test was used. Reliable difference was considered at p ≤ 0.05. These studies found an increase of the total number of polymorphonuclear neutrophils in peripheral blood, while reducing their cytotoxicity parameters. We observed reduced myeloperoxidase activity and level of cationic proteins. Reducing in the levels of CD15 and CD95 expression on the background of increasing polymorphonuclear neutrophils activity in the spontaneous NBT-test may indicate a decrease in ability to complete phagocytosis. The dynamics of CD11a and CD11b expression in polymorphonuclear neutrophils was multidirectional, and the changes were not statistically significant. Polymorphonuclear neutrophils membrane stiffness decreased in stage IV ovarian cancer. We also observed the levels of IL-1β reduced up to 248.5 ± 9.00 pg / ml in stage III ovarian cancer, up to 230.9 ± 15.04 pg / ml in stage IV ovarian cancer vs 36.8 ± 10.74 pg / ml in control, reduced levels of 1Ra, and IL-18. We found that the level of IL-6 increased at stage III ovarian cancer up to 34.38 ± 9.74 pg / ml vs 7.66 ± 2.28 pg / ml in control, and the levels of TNF-α and G-CSF decreased. Thus, these results suggest a state of secondary immunodeficiency in patients with ovarian cancer at III-IV clinical stages of the disease.

The state of cytokine status in patients with mechanical jaundice of benign origin

The results of survey of 67 patients with obstructive jaundice (OJ) of benign origin are analyzedin the article. In all patients the cause of obstructive jaundice was choledocholithiasis. The degree of hepatic dysfunction (HD) was identified taking into account of cholestasis (total serum bilirubin and alkaline phosphatase activity in blood) and cytolysis syndromes (levels of alanine aminotransferase and aspartate aminotransferase). Liver dysfunction degree I was observed in 21 patients, degree II--in 14, degree III--in 10 and degree IV--in 9 patients. In 13 patients OJ was complicated with purulent cholangitis (PCh). Serum concentration of TNF-α, IFN-γ, IL-2, IL-4, IL-6, IL-10 was determined. Cytokine status imbalance was diagnosed in all patients with benign mechanical jaundice on admission. Levels of serum proinflammatory cytokines TNF-α, IFN-γ and IL-6 were greater and the level of IL-2 was lower than those in healthy people. The analysis has showed that severity of cytokine imbalance depends on the degree of LD and level of hyperbilirubinemia. Imbalance in cytokine regulation manifests in disorders of pro- and anti-inflammatory cytokinesratio.

Investigation of the cytokine status in patients during the acute period of intracranial arterial aneurysm rupture

Objective: Analyze the changes of -1β, -4, -6 interleukin indices in blood serum of patients during the acute period of intracranial arterial aneurysm (AA) rupture.Materials and methods: Levels of -1β, -4, -6 interleukins in the serum were analyzed in 37 patients during the acute period of intracranial arterial aneurysm (AA) rupture with the help of immune-enzyme analysis: in 27 patients on the 1–4th day since the manifestation of the disease; in 22 patients — on the 7–10th day after the haemorrhage (in 10 — recurrent). Clinical and instrumental examination of all the patients included brain CCT, cerebral angiography, ultrasonography; microsurgical exclusion of aneurysm was carried out according to indications.Results: The interleukin indices in the serum of patients with SAH were higher than those in the control group mainly on the 7–10th day after the SAH episode.The interleukin-6 level was 20–30 times higher compared to the average index of the control group. There was a reliable increase of its level in patients with cerebral vasospasm (VS) compared to those without VS (p&lt;0,05). The interleukins-1β and -4 indices were moderately (2–5 times) increased in comparison with those in the control group, they did not differ significantly in patients with VS and without it. In patients likely to have grave incapacitation or fatal outcome interleukin-6 levels were significantly higher both during the first 4 days of the disease (p&lt;0,05), and on the 7–10th day.Conclusions: The increased levels of -1β, -4 та -6 interleukins during the first 10 days after AA rupture indicate that there exist active inflammatory processes in this period of the disease, which gives grounds for the use of antiinflammatory therapy. A connection established between the increase of interleukin-6 level, presence of cerebral VS, as well as severity of the disease may be evidence of this cytokine’s significance in SAH.

Особенности нарушений коагуляционного потенциала крови в условиях развития системного воспалительного ответа при гнойных тубоовариальных образованиях

Objective: There are no any systemized studies of relationship between the coagulative haemostasis’ disorders and metabolic and cytokine status in patients with septic tuboovarial formations. The aim of the present work was to study the mechanisms of blood coagulation disorders and their relationships with changes of cytokine status and acute phase of inflammatory response in septic tubovarial formations in women. Methods: 32 patients with purulent tubovarial formations and 30 healthy women were examined. Results: Shortening of activated partial thromboplastin, prothrombin and thrombin clotting time, increasing the duration of XIIa-kallikrein-dependent fibrinolysis, as well as the elevation of paracoagulation products in blood plasma were observed. IL-1β (p =0.000023), TNF-α (р &lt;0.001), C-reactive protein (р &lt;0.001), haptoglobin (р &lt;0.001) and fibrinogen (р &lt;0.001) levels were higher in peripheral blood of patients in comparison with healthy women. Accumulation of lipid hydroperoxides (р &lt;0.001) and malonic dialdehyde (р &lt;0.001) occurred in the blood plasma of patients. Serum albumin (р &lt;0.001) and transferring (р &lt;0.001) levels were lesser in patients with purulent tuboovarial formations in comparison with healthy women. Conclusion: The obtained results showing an initiating role of cytokine and oxidative metabolic status changes in blood coagulation potential’s and fibrinolysis activity’s disorders developing. This biochemical signs may be used as objective criteria which may serve to determine the risk of thrombosis in case of acute inflammatory response in women with purulent tubovarial inflammation.

VASCULAR AND CARDIAC REMODELLING AND COMbINATION THERAPY OF ARTERIAL HYPERTENSION IN PATIENTS WITH CHRONIC ObSTRUCTIvE PULMONARY DISEASE

Aim. To assess cardiac and vascular structure and function, blood flow parameters, endothelial dysfunction, and cytokine status in patients with arterial hypertension (Ah) and chronic obstructive pulmonary disease (COPD), who were administered combination Co-renitec therapy. Material and methods.The study included 37 patients with Ah and COPD, who underwent laboratory, functional, and ultrasound examination. Results.During the 6-month combination Co-renitec therapy, the parameters of clinical status, circadian blood pressure profile, and endothelial, vascular, and cardiac structure and function were assessed in patients with Ah and COPD. Conclusion. The combination Co-renitec therapy was associated with normalised blood pressure levels, improved clinical and hemodynamic status, cardiac and vascular structure and function, and blood flow and microcirculation, as well as with reduced levels of the markers of endothelial dysfunction and systemic inflammation.

Immune and cytokine disorders in patients with chronic foot osteomyelitis

Aim. To study the cell and humoral immunity and cytokine status in patients with chronic foot osteomyelitis associated with diabetes mellitus and atherosclerosis. Methods. Immune and cytokine status was assessed in 20 patients with chronic foot osteomyelitis. Results were compared to control group of 20 healthy blood donors. Patients underwent clinical examination, lymphocyte populations phenotypes, phagocytes, immunoglobulins and interleukins concentration were studied. Statistical analysis was performed using «Excel» and «Statistica 10» software. To compare immunological parameters, Newman-Keuls test was used. Results. Development of foot osteomyelitis was associated with decreasing of phagocytic activity, absolute and relative number of T-helpers, NK-cells, increasing of T-cytotoxic cells number, pro- and anti-inflammatory cytokines levels. Conclusion. A marked suppression of cell-mediated immunity and increased blood levels of pro- and antiinflammatory cytokines were found in patients with chronic foot osteomyelitis. Standard treatment decreased, but did not normalize the immune and cytokine imbalance.