Detection Of Cytokine mRNA Research Articles

Assessment of pain after subcutaneous injection of erythropoietin in patients receiving haemodialysis.

Abstract Optimal T cell activation requires antigen recognition through the T cell receptor (TCR), engagement of costimulatory molecules, and cytokines. T cells can also directly recognize danger signals through the expression of toll-like receptors (TLRs). Whether TLR ligands have the capacity to provide costimulatory signals and enhance antigen-driven T cell activation is not well understood. Here, we show that TLR2 and TLR7 ligands potently lower the antigen threshold for cytokine production in T cells. To investigate how TLR triggering supports cytokine production, we adapted the protocol for flow cytometry-based fluorescence in situ hybridization (Flow-FISH) to mouse T cells. The simultaneous detection of cytokine mRNA and protein with single-cell resolution revealed that TLR triggering primarily drives de novo mRNA transcription. Ifng mRNA stabilization only occurs when the TCR is engaged. TLR2, but not TLR7-mediated costimulation, can enhance mRNA stability at low antigen levels. Importantly, TLR2 costimulation increases the percentage of polyfunctional T cells, a hallmark of potent T cell responses. In conclusion, TLR-mediated costimulation effectively potentiates T cell effector function to suboptimal antigen levels.

Detection of Cytokine mRNA in Polyclonally‐, Antigen‐ or Allergen‐Stimulated Mononuclear Cells

Immunological ReviewsVolume 119, Issue 1 p. 147-161 Detection of Cytokine mRNA in Polyclonally-, Antigen- or Allergen-Stimulated Mononuclear Cells Jean-François Gauchat, Corresponding Author Jean-François Gauchat Institute of Clinical Immunology, University of Bern, Inselpital, Bern, Switzerland.Address correspondence and reprint requests to: Dr. J.-F. Gauchal. DNAX Research Institute, Human Immunology Department, 901 California Avenue, Palo Alto, CA 94304-1104, U.S.A.Search for more papers by this authorDominique Gauchat, Dominique Gauchat Institute of Clinical Immunology, University of Bern, Inselpital, Bern, Switzerland.Search for more papers by this authorGang Qiu, Gang Qiu Institute of Clinical Immunology, University of Bern, Inselpital, Bern, Switzerland.Search for more papers by this authorMichèle Mandallaz, Michèle Mandallaz Institute of Clinical Immunology, University of Bern, Inselpital, Bern, Switzerland.Search for more papers by this authorBeda M. Stadler, Beda M. Stadler Institute of Clinical Immunology, University of Bern, Inselpital, Bern, Switzerland.Search for more papers by this author Jean-François Gauchat, Corresponding Author Jean-François Gauchat Institute of Clinical Immunology, University of Bern, Inselpital, Bern, Switzerland.Address correspondence and reprint requests to: Dr. J.-F. Gauchal. DNAX Research Institute, Human Immunology Department, 901 California Avenue, Palo Alto, CA 94304-1104, U.S.A.Search for more papers by this authorDominique Gauchat, Dominique Gauchat Institute of Clinical Immunology, University of Bern, Inselpital, Bern, Switzerland.Search for more papers by this authorGang Qiu, Gang Qiu Institute of Clinical Immunology, University of Bern, Inselpital, Bern, Switzerland.Search for more papers by this authorMichèle Mandallaz, Michèle Mandallaz Institute of Clinical Immunology, University of Bern, Inselpital, Bern, Switzerland.Search for more papers by this authorBeda M. Stadler, Beda M. Stadler Institute of Clinical Immunology, University of Bern, Inselpital, Bern, Switzerland.Search for more papers by this author First published: February 1991 https://doi.org/10.1111/j.1600-065X.1991.tb00582.xCitations: 6AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume119, Issue1February 1991Pages 147-161 RelatedInformation