Cytochrome F420 Research Articles

Endosymbiotic Actinidic Archaeal Generation of Ammonia and Thiocyanate Regulates Cell/Neuroimmunoendocrine System and Provides a Substrate for Archaeal Energetics

Aims and Objectives: Actinidic archaea has been related to the pathogenesis of schizophrenia, malignancy, metabolic syndrome x, autoimmune disease and neuronal degeneration. Actinidic archaea use cholesterol as a carbon and energy source. Archaeal cholesterol catabolism mediated by archaeal cholesterol oxidase can produce cholesterol ring oxidation to generate pyruvate. Pyruvate is converted to glutamate and ammonia can be generated from it. Archaeal urease can act upon urea generating ammonia and thiocyanate. Ammonia and thiocyanate serves the purpose of cellular and neuroimmune endocrine regulation. The archaea are ammonia oxidizing and can use ammonia for their energetics. The archaeal urease activity related ammonia and thiocyanate synthesis as well as cholesterol oxidase activity generating pyruvate and ammonia was studied in schizophrenia, malignancy, metabolic syndrome x, autoimmune disease and neuronal degeneration. Methodology: The following groups were included in the study: - endomyocardial fibrosis, alzheimer’s disease, multiple sclerosis, non-hodgkin’s lymphoma, metabolic syndrome x with cerebrovascular thrombosis and coronary artery disease, schizophrenia, autism, seizure disorder, creutzfeldt jakob disease and acquired immunodeficiency syndrome. Plasma from fasting heparinised blood was used and the experimental protocol was as follows (I) Plasma+phosphate buffered saline, (II) same as I+cholesterol substrate, (III) same as II+rutile 0.1 mg/ml, (IV) same as II+ciprofloxacine and doxycycline each in a concentration of 1 mg/ml. The following estimations were carried out:- Cytochrome F420, hydrogen peroxide, pyruvate, ammonia, glutamate, thiocyanate and urease activity. Results: Plasma of control subjects showed increased levels of the above mentioned parameters with after incubation for 1 hour and addition of cholesterol substrate resulted in still further significant increase in these parameters. The plasma of patients showed similar results but the extent of increase was more. The addition of antibiotics to the control plasma caused a decrease in all the parameters while addition of rutile increased their levels. The addition of antibiotics and rutile to the patient’s plasma produced the same changes but the extent of change was more in patient’s sera as compared to controls. Conclusion: An actinide dependent shadow biosphere of archaea and viroids in the above mentioned disease states is described. The archaeal urease generates thiocyanate and ammonia. The archaeal cholesterol oxidase catabolises cholesterol to generate pyruvate which is converted to glutamate and ammonia. Ammonia functions as a possible gasotransmitter in the brain. Ammonia can regulate mitochondrial function, membrane sodium potassium ATPase activity and immunity. It plays a role in the pathogenesis of schizophrenia, malignancy, metabolic syndrome x, autoimmune disease and neuronal degeneration. Ammonia and thiocyanate serves the purpose of cellular/neuroimmuneendocrine regulation. The archaea can utilize ammonia oxidation for energetics. Key words : Actinides; Archaea; Urease; Cholesterol Oxidase; Ammonia; Thiocyanate

Endosymbiotic Actinidic Archaea and Viroids -- A Model for Abiogenesis and Viral, Prokaryote, Eukaryotic, Primate and Human Evolution

Aim: A hypothesis regarding endosymbiotic actinidic archaea as having evolved from isoprenoid organisms by abiogenesis is presented in this paper. An actinidc archaea/ viroid mediated model of prokaryote, viral, eukaryotic, primate and human evolution is discussed. Endomyocardial fibrosis along with the root wilt disease of coconut is endemic to Kerala with its radioactive actinide beach sands. Actinides like rutile and organisms like phytoplasmas and viroids have been implicated in the etiology of these diseases. Actinidic archaea has been related to the pathogenesis of schizophrenia, malignancy, metabolic syndrome x, autoimmune disease and neuronal degeneration. Actinidic archaea have a mevalonate pathway and cholesterol catabolism. A hypothesis of cholesterol as the primal prebiotic molecule synthesised on actinide surfaces with all other biomolecules arising from it and a self replicating cholesterol lipid organism as the initial life form is presented. A cholesterol based theory of abiogenesis and its role in the evolution of actinidic archaea is discussed. The origin of viruses, prokaryotes, eukaryotes, primates and humans from the initial isoprenoid organism derived actinidic archaea is postulated. Methods: Plasma from fasting heparinised blood was used and the experimental protocol was as follows (I) Plasma+phosphate buffered saline, (II) same as I+cholesterol substrate, (III) same as II+rutile 0.1 mg/ml, (IV) same as II+ciprofloxacine and doxycycline each in a concentration of 1 mg/ml. The following estimations were carried out:- Cytochrome F420, free RNA, free DNA, polycyclic aromatic hydrocarbon, hydrogen peroxide, dopamine, serotonin, pyruvate, ammonia, glutamate, cytochrome C, hexokinase, ATP synthase, HMG CoA reductase, digoxin and bile acids. Results: Plasma of control subjects showed increased levels of the above mentioned parameters with after incubation for 1 hour and addition of cholesterol substrate resulted in still further significant increase in these parameters. The plasma of patients showed similar results but the extent of increase was more. The addition of antibiotics to the control plasma caused a decrease in all the parameters while addition of rutile increased their levels. The addition of antibiotics and rutile to the patient’s plasma produced the same changes but the extent of change was more in patient’s sera as compared to controls. Conclusion: An actinide dependent shadow biosphere of archaea and viroids in the above mentioned disease states is described. Metal actinides in beach sands have been postulated to play a role in abiogenesis. Cholesterol is the primal prebiotic molecule synthesised on actinide surfaces with all other biomolecules arising from it. A self replicating cholesterol lipid organism could be the initial life form. A cholesterol based abiogenesis is a more likely evolutionary option and the actinidic archaea and viroids would have evolved from it. The origin of viruses, prokaryotes, eukaryotes, primates and humans from the initial isoprenoid organism derived actinidic archaea is discussed. Key words : Actinide; Archaea; Viroid; Eukaryote; Prokaryote; Virus; Primate; Human; Evolution

Endosymbiotic Actinidic Archaeal Cholesterol Catabolic Syndrome – Hypocholesterolemia and Human Diseases

Aims and Objectives: Actinidic archaea have been implicated in the pathogenesis of schizophrenia, malignancy, metabolic syndrome x, autoimmune disease and neuronal degeneration. Actinide based primitive organism like archaea have a mevalonate pathway and cholesterol catabolism. Cholesterol catabolism by actinidic archaea can lead to cholesterol depletion and a hypocholesterolemic state contributing to the pathogenesis of these disorders. A endosymbiotic actinidic archaeal cholesterol catabolic syndrome is described. Methodology: Plasma from fasting heparinised blood was used and the experimental protocol was as follows (I) Plasma+phosphate buffered saline, (II) same as I+cholesterol substrate, (III) same as II+rutile 0.1 mg/ ml, (IV) same as II+ciprofloxacine and doxycycline each in a concentration of 1 mg/ml. The following estimations were carried out:- Cytochrome F420, cholesterol oxidase activity measured by hydrogen peroxide liberation, digoxin, bile acid, polycyclic aromatic hydrocarbon, pyruvate, butyrate and propionate were estimated. Results: Plasma of control subjects showed increased levels of the above mentioned parameters with after incubation for 1 hour and addition of cholesterol substrate resulted in still further significant increase in these parameters. The plasma of patients showed similar results but the extent of increase was more. The addition of antibiotics to the control plasma caused a decrease in all the parameters while addition of rutile increased their levels. The addition of antibiotics and rutile to the patient’s plasma produced the same changes but the extent of change was more in patient’s sera as compared to controls. Conclusion: An actinide dependent shadow biosphere of archaea in the above mentioned disease states is described. The archaea can catabolize cholesterol to generate pyruvate, butyrate, propionate, polycyclic aromatic hydrocarbons, bile acids, digoxin and hydrogen peroxide. Archaeal cholesterol catabolism leads to the generation of a hypocholesterolemic contributing to the pathogenesis of schizophrenia, malignancy, metabolic syndrome x, autoimmune disease and neuronal degeneration. This can be described as the endosymbiotic actinidic archaeal cholesterol catabolic syndrome. Key words : Actinides; Archaea; Cholesterol Catabolism; Cholesterol aromatase; Cholesterol side chain oxidase; Cholesterol ring oxidase; Beta hydroxysteroid dehydrogenase

Endosymbiotic Archaeal Generated RNA Viroids Can Regulate Cell Function and Contribute to Disease State - Role in Viral Speciation

Aim Endomyocardial fibrosis along with the root wilt disease of coconut is endemic to Kerala with its radioactive actinide beach sands. Actinides like rutile as well as organisms like phytoplasmas and viroids have been implicated in the etiology of these diseases. RNA viroids has been related to the pathogenesis of schizophrenia, malignancy, metabolic syndrome x, autoimmune disease and neuronal degeneration. The possibility of archaeal RNA viroids contributing to the pathogenesis of these disease states was studied. Methods Plasma from fasting heparinised blood was used and the experimental protocol was as follows (I) Plasma+phosphate buffered saline, (II) same as I+cholesterol substrate, (III) same as II+rutile 0.1 mg/ml, (IV) same as II+ciprofloxacine and doxycycline each in a concentration of 1 mg/ml. The following estimations were carried out:- Cytochrome F420, free RNA and free DNA. Results Plasma of control subjects showed increased levels of the above mentioned parameters with after incubation for 1 hour and addition of cholesterol substrate resulted in still further significant increase in these parameters. The plasma of patients showed similar results but the extent of increase was more. The addition of antibiotics to the control plasma caused a decrease in all the parameters while addition of rutile increased their levels. The addition of antibiotics and rutile to the patient’s plasma produced the same changes but the extent of change was more in patient’s sera as compared to controls. Conclusion An actinide dependent shadow biosphere of archaea and viroids in the above mentioned disease states is described. Actinidic archaea generated RNA viroids can regulate cell function and modulate the neuro-immunoendocrine system by the mechanism of RNA interference. The archaea generated RNA viroids can also contribute to the pathogenesis of schizophrenia, malignancy, metabolic syndrome x, autoimmune disease and neuronal degeneration. The RNA viroids could play a role in viral speciation by recombining with endogenous commensal viruses. Key words : Actinide; Archaea; Viroid; Cell function

Endosymbiontic Actinidic Archaeal Synthesis of Digoxin from Cholesterol Regulates Cellular Function and Contributes to Disease Pathology

Aims and Objectives: Endomyocardial fibrosis along with the root wilt disease of coconut is endemic to Kerala with its radioactive actinide beach sands. Actinides like rutile, endogenous digoxin as well as organisms like phytoplasmas and viroids have been implicated in the etiology of these diseases. Endogenous digoxin has been related to the pathogenesis of schizophrenia, malignancy, metabolic syndrome x, autoimmune disease and neuronal degeneration. The possibility of endogenous digoxin synthesis by actinide based primitive organism like archaea with a mevalonate pathway and cholesterol catabolism was considered in these disease states. Methodology: Plasma from fasting heparinised blood was used and the experimental protocol was as follows (I) Plasma+phosphate buffered saline, (II) same as I+cholesterol substrate, (III) same as II+rutile 0.1 mg/ml, (IV) same as II+ciprofloxacine and doxycycline each in a concentration of 1 mg/ml. The following estimations were carried out:- Cytochrome F420 and digoxin. Results: Plasma of control subjects showed increased levels of the above mentioned parameters with after incubation for 1 hour and addition of cholesterol substrate resulted in still further significant increase in these parameters. The plasma of patients showed similar results but the extent of increase was more. The addition of antibiotics to the control plasma caused a decrease in all the parameters while addition of rutile increased their levels. The addition of antibiotics and rutile to the patient’s plasma produced the same changes but the extent of change was more in patient’s sera as compared to controls. Conclusion: Actinidic archaea can synthesize digoxin from cholesterol. Archaeal digoxin place an important role in neuroimmunoendocrine integration and can contribute to the above mentioned disease states. Key words : Archaea; Actinide; Digoxin; Cholesterol; Disease

Actinidic Archaea Mediates Biological Transmutation in Human Systems- Experimental Evidence

Abstract Background: Actinidic archaea has been described in human systems from our laboratory and function as cellular endosymbionts regulating multiple cellular functions. The actinidic archaea is an endosymbiont of the human cell and it is possible that the organism can mediate biological transmutation. The actinidic archaea can exist as nanoarchaea which can undergo magnetite and calcium mineralization. It is possible that magnesium is being transmuted biologically to calcium to produce amounts sufficient for calcium mineralization. Calcified nanoarchaea can produce a systemic immune activation contributing to the diverse pathologies. Methods: Experimental system for demonstrating biological transmutation by archaea was as follows: The basic system contained patient’s serum 0.5 ml + normal serum 0.25 ml + physiological buffered saline + cerium chloride 0.1 mg/ml. To the basic system MgSO4 0.1 mg/ ml was added. The Mg++ and Ca++ were estimated at 0 hour. The remaining portion was incubated for 16 hours at 37 oC for 16 hours. The Mg++ and Ca++ were estimated at the end of 16 hours. The estimation of Mg++ and Ca++ were done by using commercial kits. Cytochrome F420 was estimated flourimetrically (excitation wavelength 420 nm and emission wavelength 520 nm). Results: The results showed that there was a decrease in magnesium and a concomitant increase in calcium in incubated serum samples from normal individuals. The percentage decrease in magnesium was 15.68 to 31.48%. The percentage increase in calcium was 10.43 to 9.79%. There was detection of cytochrome F420 in the system by fluorescence indicating archaeal growth dependent on actinidic cerium. This showed that the actinidic archaea was mediating the biological transmutation of magnesium to calcium. Conclusions: The actinidic archaea can mediate magnesium to calcium transmutation. The transmutation altered calcium-magnesium ratios in the cell can alter synaptic transmission, mitochondrial function, golgi body/ ER function, lysosomal function, immune activation, cell proliferation, insulin resistance and cell death. Key words : Archaea; Actinides; Calcium; Magnesium; Transmutation

Endosymbiotic Actinidic Archaea and Viroids Regulate Cellular Organelle Function, Cell Growth, Cell Differentiation and Cell Death

Background: A hypothesis regarding the role of endosymbiotic actinidic archaea and viroids in the regulation of cell function, cell differentiation, cell proliferation and cell death is discussed. Endomyocardial fibrosis (EMF) along with the root wilt disease of coconut is endemic to Kerala with its radioactive actinide beach sands. Actinides like rutile producing intracellular magnesium deficiency due to rutile-magnesium exchange sites in the cell membrane has been implicated in the etiology of EMF. Organisms like phytoplasmas and viroids have also been demonstrated to play a role in the etiology of these diseases. Actinidic archaea and viroids have been related to the pathogenesis of malignancies and degenerations. The actinidic archaea have a mevalonate pathway and cholesterol catabolism. An actinide dependent shadow biosphere of archaea and viroids regulating the cell cycle is described in the above mentioned disease states. Methods: The following groups were included in the study:- Group A: Non-hodgkin’s B cell lymphoma, multiple myeloma and cns glioma- glioblastoma multiforme. Group B :- Alzheimer’s disease, Parkinson’s disease, Huntington’s disease and Motor neuron disease. There were 10 patients in each group and each patient had an age and sex matched healthy control selected randomly from the general population. Cholesterol substrate was added to the plasma of the patients and the generation of cytochrome F420, free RNA, free DNA, polycyclic aromatic hydrocarbon, hydrogen peroxide, pyruvate, ammonia, glutamate, cytochrome C, hexokinase, ATP synthase, HMG CoA reductase, digoxin and bile acids were studied. The changes with the addition of antibiotics and rutile to the patient’s plasma were also studied. Results: The parameters mentioned above were increased the patient’s plasma with addition of cholesterol substrate. The addition of antibiotics to the patient’s plasma caused a decrease in all the parameters while addition of rutile increased their levels. Conc lus ion: An a c t inide dependent shadow biosphere of archaea and viroids is described in nonhodgkin’s B cell lymphoma, multiple myeloma and CNS glioma- glioblastoma multiforme producing the Warburg phenotype contributing to their pathogenesis. Actinidic archaea and viroids can also contribute to the cell death in Alzheimer’s disease, Parkinson’s disease, Huntington’s disease and Motor neuron disease. The role of actinidic archaea and viroids as well as endogenous digoxin in regulation of cell function, cell differentiation, cell proliferation and cell death is discussed. The endosymbiotic actinidic archaea and viroids can regulate the cell cycle and cellular organelle function. Key words : Archaea; Viroids; Cholesterol; Actinide; Non-Hodgkin’s Lymphoma; Multiple Myeloma; CNS Glioma; Alzheimer’s Disease; Parkinson’s Disease; Huntington’s Disease; Motor Neuron Disease; Cell Cycle; Organelle

Endosymbiotic Actinidc Archaea/Viroids, Quantal Perception and Biological Reincarnation

Objective: An endosymbiotic actinidic archaea mediated model of quantal perception mediated biological reincarnation is presented. Actinidic archaea has been related to the pathogenesis of schizophrenia, autism and primary seizure disorder. Actinidic archaea have a mevalonate pathway and cholesterol catabolism. The role of actinidic archaea and viroids in quantal perception and biological reincarnation is evaluated. Methods: 10 cases each of schizophrenia, autism and primary seizure disorder before starting treatment and 10 age and sex matched healthy controls from general population were chosen for the study. Cholesterol substrate was added to the plasma of the patients and the generation of Cytochrome F420, free RNA, free DNA, polycyclic aromatic hydrocarbon, hydrogen peroxide, dopamine, noradrenaline, serotonin, pyruvate, ammonia, glutamate, acetyl choline, hexokinase, HMG CoA reductase, digoxin and bile acids were studied. The changes with the addition of antibiotics and rutile to the patient’s plasma were also studied. The statistical analysis was done by ANOVA. Results: The parameters mentioned above were increased the patient’s plasma with addition of cholesterol substrate. The addition of antibiotics to the patient’s plasma caused a decrease in all the parameters while addition of rutile increased their levels. Conclusion: An actinide dependent shadow biosphere of archaea and viroids is described in schizophrenia, autism and primary seizure disorder contributing to their pathogenesis. An endosymbiotic actinidic archaea and viroid mediated model of quantal perception and biological reincarnation is elucidated in this paper. The endosymbiotic actinidic archaea and viroids have got axonal and transynaptic transport functioning as biological neurotransmitters. The human brain can be compared to a well organised modified archaeal biofilm with archaeal derived viroids serving as messengers. The actinidic archaea with its magnetite can mediate quantal perception and store biological information. The actinidic archaea are eternal and the biological information stored in archaeal magnetite quantal computers may serve as a store of biological information in nature. The actinidic archaeal magnetite mediated quantal perception also forms the basis of the collective unconscious. This can mediate the mechanism of reincarnative memories. Key words : Conscious perception; Quantal perception; Archaea; Viroids; Cholesterol; Actinide; Reincarnative memory

Endosymbiotic Actinidic Archaea and Viroids Mediated Model of Conscious/ Quantal Perception and Regulation of Brain Function

Objective: An endosymbiotic actinidic archaea and viroid mediated model of conscious and quantal perception is presented. Actinidic archaea has been related to the pathogenesis of schizophrenia, autism and primary seizure disorder. Actinidic archaea have a mevalonate pathway and cholesterol catabolism. The role of actinidic archaea derived viroids in conscious/quantal perception is evaluated. Methods: 10 cases each of schizophrenia, autism and primary seizure disorder before starting treatment and 10 age and sex matched healthy controls from general population were chosen for the study. Cholesterol substrate was added to the plasma of the patients and the generation of Cytochrome F420, free RNA, free DNA, polycyclic aromatic hydrocarbon, hydrogen peroxide, dopamine, noradrenaline, serotonin, pyruvate, ammonia, glutamate, acetyl choline, hexokinase, HMG CoA reductase, digoxin and bile acids were studied. The changes with the addition of antibiotics and rutile to the patient’s plasma were also studied. The statistical analysis was done by ANOVA. Results: The parameters mentioned above were increased the patient’s plasma with addition of cholesterol substrate. The addition of antibiotics to the patient’s plasma caused a decrease in all the parameters while addition of rutile increased their levels. Conclusion: An actinide dependent shadow biosphere of archaea and viroids is described in schizophrenia, autism and primary seizure disorder contributing to their pathogenesis. An endosymbiotic actinidic archaea and viroid mediated model of conscious and quantal perception is elucidated in this paper. The endosymbiotic actinidic archaea and viroids have got axonal and transynaptic transport functioning as biological neurotransmitters. The human brain can be compared to a well organised modified archaeal biofilm with archaeal derived viroids serving as messengers. Key words : Conscious perception; Quantal perception; Archaea; Viroids; Cholesterol; Actinide

Endosymbiotic Actinidic Archaea and Viroids- Role in Metabolic/Endocrine Regulation

Objective: A hypothesis regarding the role of endosymbiotic actinidic archaea and viroids in metabolic/ endocrine regulation is put forward. Endogenous digoxin has been related to the pathogenesis of metabolic syndrome x with type 2 diabetes mellitus and vascular disease. Elevated digoxin levels are described in endomyocardial fibrosis and root wilt disease of coconut seen in same endemic zones with phytoplasmas, rutile and viroids important in their pathogenesis. The possibility of endogenous digoxin synthesis by actinide based primitive organism like archaea with a mevalonate pathway and cholesterol catabolism was considered. The role of archaeal derived virioids in metabolic and endocrine regulation was also evaluated. Research design and methods: 10 cases each of type 2 diabetes mellitus, coronary artery disease- acute coronary syndrome and acute cerebrovascular thrombosis before starting treatment and 10 age and sex matched healthy controls from general population were chosen for the study. The CAD and CVA patients were nondiabetics. Cholesterol substrate was added to the plasma of the patients and the generation of cytochrome F420, free RNA, free DNA, polycyclic aromatic hydrocarbon, hydrogen peroxide, serotonin, pyruvate, ammonia, glutamate, cytochrome C, hexokinase, ATP synthase, HMG CoA redutase, digoxin and bile acids were studied. The changes with the addition of antibiotics and rutile to the patient’s plasma were also studied. The statistical analysis was done by ANOVA. Results: The parameters mentioned above were increased the patient’s plasma with addition of cholesterol substrate. The addition of antibiotics to the patient’s plasma caused a decrease in all the parameters while addition of rutile increased their levels. Conclusions: An actinide dependent shadow biosphere of archaea and viroids is described in type 2 diabetes mellitus, coronary artery disease- acute coronary syndrome and acute cerebrovascular thrombosis contributing to their pathogenesis. Endosymbiotic actinidic archaea and viroids plays a pivotal role in metabolic/endocrine regulation. Key words : Archaea; Viroids; Cholesterol; Actinide; Metabolic and Endocrine Regulation

Endosymbiotic Actinidic Archaea and Viroids-Role in Genomic Regulation

Objective: A hypothesis regarding the role of endosymbiotic actinidic archaea and viroids in immune regulation is put forward. Endogenous digoxin has been related to the pathogenesis of multiple sclerosis and other autoimmune diseases like systemic lupus erythematosis and rheumatoid arthritis. The possibility of endogenous digoxin synthesis by actinidic archaea with a mevalonate pathway and cholesterol catabolism was considered. The role of archaeal derived viroids in immune regulation was also evaluated. Methods: 10 cases each of multiple sclerosis and other autoimmune diseases like systemic lupus erythematosis and rheumatoid arthritis before starting treatment and 10 age and sex matched healthy controls from general population were chosen for the study. Cholesterol substrate was added to the plasma of the patients and the generation of cytochrome F420, free RNA, free DNA, polycyclic aromatic hydrocarbon, hydrogen peroxide, serotonin, pyruvate, ammonia, glutamate, cytochrome C, hexokinase, ATP synthase, HMG CoA reductase, digoxin and bile acids were studied. The changes with the addition of antibiotics and rutile to the patient’s plasma were also studied. The statistical analysis was done by ANOVA. Results: The parameters mentioned above were increased the patient’s plasma with addition of cholesterol substrate. The addition of antibiotics to the patient’s plasma caused a decrease in all the parameters while addition of rutile increased their levels. Conclusions: An actinide dependent shadow biosphere of archaea and viroids is described in multiple sclerosis and other autoimmune diseases like systemic lupus erythematosis and rheumatoid arthritis contributing to their pathogenesis. The actinidic archaea and viroids play a role in immune regulation. Key words : Archaea; Viroids; Cholesterol; Actinide; Multiple sclerosis; Systemic lupus erythematosis; Rheumatoid arthritis; Immune regulation

Endosymbiotic Actinidic Archaea/Viroids, Hemispheric Dominance and the Tridosha Theory

Objective: Endogenous digoxin has been related to hemispheric dominance. Right hemispheric dominant individuals were hyperdigoxinemic, left hemispheric dominan t indiv idual s wer e hypodigoxinemic and bihemispheric dominant individuals were normodigoxinemic. The possibility of endogenous digoxin synthesis by actinide based primitive organism like archaea with a mevalonate pathway and cholesterol catabolism was considered. An actinide dependent shadow biosphere of archaea and viroids in normal humans and disease states is described. The intracellular endosymbionts archaea and their intron derived viroids constitute the third element regulating the human body. Ayurveda, the traditional Indian System of Medicine, deals with the theory of the three tridosha states (both physical and psychological): Vata, Pitta, and Kapha. The Kapha state has been demonstrated as equivalent to right hemispheric dominant hyperdigoxinemic state. The Pitta state has been demonstrated as equivalent to the left hemispheric dominant hypodigoxinemic state. The Vata state has been demonstrated as equivalent to the bihemispheric dominant normodigoxinemic state.8 The study assessed actinidic archaea and viroids in the tridosha states of Ayurveda. The results are presented in this paper. Methods: The following groups were included in the study:- (I) right handed-left hemispheric dominantpitta group, (II) left handed-right hemispheric dominantkapha group and (III) amphidextrous-bihemispheric dominant-vata group of individuals. Cholesterol substrate was added to the plasma of the patients and the generation of cytochrome F420, free RNA, free DNA, polycyclic aromatic hydrocarbon, hydrogen peroxide, serotonin, pyruvate, ammonia, glutamate, cytochrome C, hexokinase, ATP synthase, HMG CoA reductase, digoxin and bile acids were studied. The changes with the addition of antibiotics and rutile to the patient’s plasma were also studied. Results: The plasma of the bihemispheric dominant vata group showed detectable levels of the above mentioned parameters after incubation for 1 hour and addition of cholesterol substrate resulted in still further increase in these parameters. The addition of antibiotics to the bihemispheric dominant vata group caused a decrease in all the parameters while addition of rutile increased their levels. The plasma of right hemispheric dominant kapha group showed a significant increase in the above mentioned parameters as compared to bihemispheric dominant vata group. The addition of antibiotics to the right hemispheric dominant kapha group caused a decrease in all the parameters while addition of rutile increased their levels but the extent of change was more in right hemispheric dominant kapha group as compared to bihemispheric dominant vata group. The plasma of left hemispheric dominant pitta group showed a significant decrease in the above mentioned parameters as compared to the bihemispheric dominant vata group. The addition of antibiotics to the left hemispheric dominant pitta group caused a decrease in all the parameters while addition of rutile increased their levels but the extent of change was less in left hemispheric dominant pitta group as compared to bihemispheric dominant vata group. Conclusion: The third element formed of intracellular archaea and viroidal symbiosis determines hemispheric dominance and tridoshas. Also archaeal cholesterol synthesis and cholesterol catabolism determines hemispheric dominance and tridoshas. The archaea and viroidal density is high in right hemispheric dominant kapha group, intermediate in bihemispheric dominant vata group and low in left hemispheric dominant pitta group. Key words : Archaea; Viroids; Cholesterol; Digoxin; Hemispheric dominance; Tridoshas; Vata; Pitta; Kapha

A Cholesterol and Actinide Dependent Shadow Biosphere of Archaea and Viroids

Aims and Objectives: Endomyocardial fibrosis along with the root wilt disease of coconut is endemic to Kerala with its radioactive actinide beach sands. Actinides like rutile, endogenous digoxin as well as organisms like phytoplasmas and viroids have been implicated in the etiology of these diseases. Endogenous digoxin has been related to the pathogenesis of schizophrenia, malignancy, metabolic syndrome x, autoimmune disease and neuronal degeneration. The possibility of endogenous digoxin synthesis by actinide based primitive organism like archaea with a mevalonate pathway and cholesterol catabolism was considered in these disease states.Methodology: Plasma from fasting heparinised blood was used and the experimental protocol was as follows (I) Plasma+phosphate buffered saline, (II) same as I+cholesterol substrate, (III) same as II+rutile 0.1 mg/ml, (IV) same as II+ciprofloxacine and doxycycline each in a concentration of 1 mg/ml. The following estimations were carried out:- Cytochrome F420, free RNA, free DNA, polycyclic aromatic hydrocarbon, hydrogen peroxide, dopamine, serotonin, pyruvate, ammonia, glutamate, cytochrome C, hexokinase, ATP synthase, HMG CoA reductase, digoxin and bile acids.Results: Plasma of control subjects showed increased levels of the above mentioned parameters with after incubation for 1 hour and addition of cholesterol substrate resulted in still further significant increase in these parameters. The plasma of patients showed similar results but the extent of increase was more. The addition of antibiotics to the control plasma caused a decrease in all the parameters while addition of rutile increased their levels. The addition of antibiotics and rutile to the patient’s plasma produced the same changes but the extent of change was more in patient’s sera as compared to controls.Conclusion: An actinide dependent shadow biosphere of archaea and viroids in the above mentioned disease states is described. Key words: Endomyocardial fibrosis; Cholesterol; Actinide; Biosphere of archaea and viroids

A Cholesterol and Actinide Dependent Shadow Biosphere of Archaea and Viroids Indicating Cholesterol Based Abiogenesis- Evolution of the Biological Universe

AimEndomyocardial fibrosis along with the root wilt disease of coconut is endemic to Kerala with its radioactive actinide beach sands. Actinides like rutile, endogenous digoxin as well as organisms like phytoplasmas and viroids have been implicated in the etiology of these diseases. Endogenous digoxin has been related to the pathogenesis of schizophrenia, malignancy, metabolic syndrome x, autoimmune disease and neuronal degeneration. The possibility of endogenous digoxin synthesis by actinide based primitive organism like archaea with a mevalonate pathway and cholesterol catabolism was considered. MethodsPlasma from fasting heparinised blood was used and the experimental protocol was as follows (I) Plasma+phosphate buffered saline, (II) same as I+cholesterol substrate, (III) same as II+rutile 0.1 mg/ml, (IV) same as II+ciprofloxacine and doxycycline each in a concentration of 1 mg/ml. The following estimations were carried out:- Cytochrome F420, free RNA, free DNA, polycyclic aromatic hydrocarbon, hydrogen peroxide, dopamine, serotonin, pyruvate, ammonia, glutamate, cytochrome C, hexokinase, ATP synthase, HMG CoA reductase, digoxin and bile acids.ResultsPlasma of control subjects showed increased levels of the above mentioned parameters with after incubation for 1 hour and addition of cholesterol substrate resulted in still further significant increase in these parameters. The plasma of patients showed similar results but the extent of increase was more. The addition of antibiotics to the control plasma caused a decrease in all the parameters while addition of rutile increased their levels. The addition of antibiotics and rutile to the patient’s plasma produced the same changes but the extent of change was more in patient’s sera as compared to controls.ConclusionAn actinide dependent shadow biosphere of archaea and viroids in the above mentioned disease states is described. Metal actinides in beach sands have been postulated to play a role in abiogenesis. A hypothesis of cholesterol as the primal prebiotic molecule synthesised on actinide surfaces with all other biomolecules arising from it and a self replicating cholesterol lipid organism as the initial life form is presented. A cholesterol based theory of abiogenesis and its role in the evolution of universe is elucidated. Key words: Biological Universe; Cholesterol; Actinide

Archaeal Digoxin and Creation of Cellular Plasma State - Molecular/Cellular Electromagnetic Signal Transduction

Objective: Endogenous digoxin can produce sodium potassium ATPase inhibition, a paroxysmal depolarisation shift and a quantal pumped phonon system. It can generate electromagnetic signals of cells and molecules which can be transferred. The study was designed to test the hypothesis. Methods: System I: Phosphate buffered saline (PBS)+ cholesterol substrate + plasma + rutile (0.1 mg/ml)+digoxin (0.5 ng/ml). System II: Control phosphate buffered saline (PBS). The system is incubated at 37oC for 1 hour and during the period of incubation both system I and II are kept close to each other. Then to control PBS add cholesterol substrate and incubate both the systems at 37oC for 16-18 hours. Cytochrome F420 was estimated flourimetrically (excitation wavelength 420 nm and emission wavelength 520 nm). The detection of cytochrome F420 indicates the presence of archaea. The fluorescence of cytochrome F420 was estimated at 0 time, after 1 hour and 16-18 hours in the system I and II. The experiment was repeated 7 times and average values given.Results: The test system I after 16-18 hours of incubation showed decrease in intensity of fluorescence due to destruction of archaea by long incubation consequent to exhaustion of substrate as well as digoxin induced cell destruction. There was a 21% increase in fluroscence in system II of PBS with added cholesterol substrate after 16-18 hours of incubation. Conclusion: The experiment indicates that in the presence of digoxin induced quantal pumped phonon system the electromagnetic trace of archaea can be generated and can be transmitted to the PBS+cholesterol substrate system resulting in generation of archaea/cytochrome F420 in the system. Key words: Digoxin; Quantal state; EMF signal; Teleportation; Cytochrome F420

Actinidic Archaea and Viroids Related Hepato-Gastrointestinal Syndrome

AbstractBackground: Endogenous digoxin has been related to the pathogenesis of cirrhosis liver, ulcerative colitis, irritable bowel syndrome and peptic ulcer disease. The possibility of endogenous digoxin synthesis by archaea with a mevalonate pathway and cholesterol catabolism was considered.Methods: 10 cases each of cirrhosis liver, ulcerative colitis, irritable bowel syndrome and peptic ulcer disease before starting treatment and 10 age and sex matched healthy controls from general population were chosen for the study. Cholesterol substrate was added to the plasma of the patients and the generation of cytochrome F420, free RNA, free DNA, polycyclic aromatic hydrocarbon, hydrogen peroxide, serotonin, pyruvate, ammonia, glutamate, cytochrome C, hexokinase, ATP synthase, HMG CoA reductase, digoxin and bile acids were studied. The changes with the addition of antibiotics and cerium to the patient’s plasma were also studied. The statistical analysis was done by ANOVA.Results: The parameters mentioned above were increased the patient’s plasma with addition of cholesterol substrate. The addition of antibiotics to the patient’s plasma caused a decrease in all the parameters while addition of cerium increased their levels. Conclusions: An actinide dependent shadow biosphere of archaea and viroids is described in cirrhosis liver, ulcerative colitis, irritable bowel syndrome and peptic ulcer disease contributing to their pathogenesis. Key words: Archaea; Viroids; Cirrhosis liver; Ulcerative colitis; Irritable bowel syndrome; Peptic ulcer disease

An Actinide Dependent Shadow Biosphere of Archaea and Viroids in Neuropsychiatric Disorders

Objective: Endogenous digoxin has been related to the pathogenesis of schizophrenia, autism and primary seizure disorder. The possibility of endogenous digoxin synthesis by actinide based primitive organism like archaea with a mevalonate pathway and cholesterol catabolism was considered.Methods: 10 cases each of schizophrenia, autism and primary seizure disorder before starting treatment and 10 age and sex matched healthy controls from general population were chosen for the study. Cholesterol substrate was added to the plasma of the patients and the generation of cytochrome F420, free RNA, free DNA, polycyclic aromatic hydrocarbon, hydrogen peroxide, serotonin, pyruvate, ammonia, glutamate, cytochrome C, hexokinase, ATP synthase, HMG CoA redutase, digoxin and bile acids were studied. The changes with the addition of antibiotics and rutile to the patient’s plasma were also studied. The statistical analysis was done by ANOVA.Results: The parameters mentioned above were increased the patient’s plasma with addition of cholesterol substrate. The addition of antibiotics to the patient’s plasma caused a decrease in all the parameters while addition of rutile increased their levels. Conclusion: An actinide dependent shadow biosphere of archaea and viroids is described in schizophrenia, autism and primary seizure disorder contributing to their pathogenesis. Key words: Schizophrenia; Autism; Primary seizure disorder; Archaea; Viroids; Cholesterol; Actinide

Actinide Dependent Shadow Biosphere of Archaea and Viroids and Hemispheric Dominance

ObjectiveEndogenous digoxin has been related to hemispheric dominance. The possibility of endogenous digoxin synthesis by actinide based primitive organism like archaea with a mevalonate pathway and cholesterol catabolism was considered. Methods10 normal individuals each of right handed and left hemispheric dominant group, left handed and right hemispheric dominant group and amphidextrous and bihemispheric dominant individuals were included in the study. Cholesterol substrate was added to the plasma of the patients and the generation of cytochrome F420, free RNA, free DNA, polycyclic aromatic hydrocarbon, hydrogen peroxide, serotonin, pyruvate, ammonia, glutamate, cytochrome C, hexokinase, ATP synthase, HMG CoA reductase, digoxin and bile acids were studied. The changes with the addition of antibiotics and rutile to the patient’s plasma were also studied. ResultsThe plasma of the bihemispheric dominant group showed detectable levels of the above mentioned parameters after incubation for 1 hour and addition of cholesterol substrate resulted in still further increase in these parameters. The addition of antibiotics to the bihemispheric dominant group caused a decrease in all the parameters while addition of rutile increased their levels. The plasma of right hemispheric dominant group showed a significant increase in the above mentioned parameters as compared to bihemispheric dominance group. The addition of antibiotics to the right hemispheric dominant group caused a decrease in all the parameters while addition of rutile increased their levels but the extent of change was more in right hemispheric dominant group as compared to bihemispheric dominant group. The addition of antibiotics to the left hemispheric dominant group caused a decrease in all the parameters while addition of rutile increased their levels but the extent of change was less in left hemispheric dominant group as compared to bihemispheric dominant group. ConclusionAn actinide dependent shadow biosphere of intracellular archaea and viroids constitutes the third element regulating hemispheric dominance. Key words: Archaea; Viroids; Cholesterol; Digoxin; Hemispheric dominance

A Cholesterol and Actinide Dependent Shadow Biosphere of Archaea and Viroids in Retroviral and Prion Disease

AbstractAim: Endogenous digoxin has been related to the pathogenesis of acquired immunodeficiency syndrome and creutzfeldt jakob disease. The possibility of endogenous digoxin synthesis by archaea with a mevalonate pathway and cholesterol catabolism was considered.Methods: 10 cases each of acquired immunodeficiency syndrome and creutzfeldt jakob disease before starting treatment and 10 age and sex matched healthy controls from general population were chosen for the study. Cholesterol substrate was added to the plasma of the patients and the generation of cytochrome F420, free RNA, free DNA, polycyclic aromatic hydrocarbon, hydrogen peroxide, serotonin, pyruvate, ammonia, glutamate, cytochrome C, hexokinase, ATP synthase, HMG CoA reductase, digoxin and bile acids were studied. The changes with the addition of antibiotics and rutile to the patient’s plasma were also studied. The statistical analysis was done by ANOVA.Results: The parameters mentioned above were increased the patient’s plasma with addition of cholesterol substrate. The addition of antibiotics to the patient’s plasma caused a decrease in all the parameters while addition of rutile increased their levels. Conclusions: An actinide dependent shadow biosphere of archaea and viroids is described in acquired immunodeficiency syndrome and creutzfeldt jakob disease contributing to its pathogenesis. Key words : Archaea; Viroids; Actinide; Cholesterol; Acquired immunodeficiency syndrome; Creutzfeldt jakob disease

A Cholesterol and Actinide Dependent Shadow Biosphere of Archaea And Viroids in Autoimmune Diseases

Objective: Endogenous digoxin has been related to the pathogenesis of multiple sclerosis and other autoimmune diseases like systemic lupus erythematosis and rheumatoid arthritis. The possibility of endogenous digoxin synthesis by archaea with a mevalonate pathway and cholesterol catabolism was considered.Methods: 10 cases each of multiple sclerosis and other autoimmune diseases like systemic lupus erythematosis and rheumatoid arthritis before starting treatment and 10 age and sex matched healthy controls from general population were chosen for the study. Cholesterol substrate was added to the plasma of the patients and the generation of cytochrome F420, free RNA, free DNA, polycyclic aromatic hydrocarbon, hydrogen peroxide, serotonin, pyruvate, ammonia, glutamate, cytochrome C, hexokinase, ATP synthase, HMG CoA reductase, digoxin and bile acids were studied. The changes with the addition of antibiotics and rutile to the patient’s plasma were also studied. The statistical analysis was done by ANOVA.Results: The parameters mentioned above were increased the patient’s plasma with addition of cholesterol substrate. The addition of antibiotics to the patient’s plasma caused a decrease in all the parameters while addition of rutile increased their levels. Conclusions: An actinide dependent shadow biosphere of archaea and viroids is described in multiple sclerosis and other autoimmune diseases like systemic lupus erythematosis and rheumatoid arthritis contributing to their pathogenesis. Key words: Archaea; Viroids; Cholesterol; Actinide; Multiple sclerosis; Systemic lupus erythematosis; Rheumatoid arthritis