Hydrodynamic flow regulators are used for diversion and/or limiting excess discharge in wastewater and stormwater systems as a replacement for traditional flow throttling devices. They are highly efficient, reliable and free from common disadvantages of traditional devices. Recent research of the authors indicated that atomization of a liquid by vortex flow regulators accelerates oxygenation and prevents the putrefaction process in wastewater and storm water collection systems. The study presents experimental results of the oxygen transfer measurements for basic designs of cylindrical hydrodynamic flow regulators in a closed-circuit experimental setup at the semi-commercial scale. The oxygen mass transfer coefficient, standard oxygen transfer rate and standard aeration efficiency were determined for the range of tested configurations by the clean water test.