Five cycloartane-type triterpene glycosides were isolated from the methanol extract of the roots of Astragalus amblolepis Fischer along with one known saponin, 3- O-β-D-xylopyranosyl-16- O-β-D-glucopyranosyl-3β,6α,16β,24( S),25-pentahydroxy-cycloartane. Structures of the compounds were established as 3- O-β-D-xylopyranosyl-25- O-β-D-glucopyranosyl-3β,6α,16β,24( S),25-pentahydroxy-cycloartane, 3- O-[β-D-glucuronopyranosyl-(1 → 2)-β-D-xylopyranosyl]-25- O-β-D-glucopyranosyl-3β,6α,16β,24( S),25-pentahydroxy-cycloartane, 3- O-β-D-xylopyranosyl-24,25-di- O-β-D-glucopyranosyl-3β,6α,16β,24( S),25-pentahydroxy-cycloartane, 6- O-α-L-rhamnopyranosyl-16,24-di- O-β-D-glucopyranosyl-3β,6α,16β,24( S),25-pentahydroxy-cycloartane, 6- O-α-L-rhamnopyranosyl-16,25-di- O-β-D-glucopyranosyl-3β,6α,16β,24( S),25-pentahydroxy-cycloartane by using 1D and 2D-NMR techniques and mass spectrometry. To the best of our knowledge, the glucuronic acid moiety in cycloartanes is reported for the first time.