The aim of this study was to know and compare the attitudes towards doping among different groups involved in Spanish cycling: future physical trainers, elite cyclists, young cyclists, and cycling team managers. The sample was composed of 492 participants (23.48±7.5 years) from different cycling contexts: university students of Sport Sciences degree -SS- (n = 271; 22.04±3.3 years), Elite Cyclist’s -EC- (n = 65; 18.43±2.9 years), Young Cyclists -YC- (n = 44; 13.0±0.82 years), and Cycling Team Managers -CTM- (n = 112; 33.65±7.17 years). A cross-sectional descriptive design was carried out using the Spanish version of the Performance Enhancement Attitude Scale (PEAS) which is a 17-items six-point Likert-type scale ranging from 17 to 102 points (1= Strongly Disagree; 6= Strongly Agree). For the whole sample, the overall score was 36.12±10.09. Regarding different groups, data were as follows: SS: 34.69±9.31; EC: 35.14±8.63; YC: 37.62±11.30; CTM: 40.12±11.27. Significant differences were observed between SS and CTM (p=0.000) groups and between EC and CTM groups (p=0.006). Spanish cycling, in general, is not permissive in relation to doping. By the way, cycling team managers, the oldest group, are significantly more lenient towards doping than EC and future physical trainers (SS). It could be suggested that “something is changing positively” in relation to attitudes towards doping in Spanish cycling, being the youngest the most sensible group, so changes could be seen in the medium-long term, not immediately. Data from YC group, more permissive than SS and EC groups, support the idea that anti-doping education programmes are needed from early ages.