To explore the flow mechanism of micro-remaining oil during cyclic waterflooding in low-permeability reservoir, the displacement effect of micro-remaining oil under conventional waterflooding and different rounds of cyclic waterflooding was investigated by using a micro-visual displacement experiment based on microfluidics model. The produced ratio of different types of micro remaining oil during cyclic waterflooding was quantified, and major types of micro remaining oils that can be applied effectively during cyclic waterflooding were analyzed. Moreover, the flow mechanism of micro remaining oil during cyclic waterflooding was investigated. Research results demonstrated that clustering type remaining oil and columnar type remaining oil were major types that were applied during cyclic waterflooding. After three rounds of cyclic waterflooding, clustering-type remaining oil decreased by 2.50% on average, and columnar-type remaining oil decreased by 0.81% on average. The major mechanism of cyclic waterflooding is introduced as follows: at intervals of cyclic waterflooding, clustering type remaining oil and columnar type remaining oil exchange with water in pores, throats, and spaces between big and small pores due to the fluctuation of pressure field and effect of capillary force. As a result, oil and water redistribute. Clustering-type remaining oil and columnar-type remaining oil become movable oils.