The presence of residual stress in welded components can have a significant impact on fatigue response. Residual stresses were assessed in thick welded A36 cantilever specimens (T-specimens) at the fatigue critical area. Testing involved subjecting the T-specimens to varying bending stresses and load cycles, leading to different levels of local plasticity at the weldtoe. Extensive X-ray diffraction (XRD) measurements were conducted to analyze the residual stress distribution near the weldtoe. Residual stress states were evaluated in both as-welded specimens and those subjected to fatigue loading with strain amplitudes up to 0.001 at the weldtoe stress concentration zone. The findings suggest that fatigue loading can induce changes in the welding-induced residual stress field, ranging from moderate to significant alterations. Therefore, fatigue life predictions should consider the potential evolution of residual stresses due to cyclic loading, including the effects of cyclic stress relaxation in fatigue life calculations.