Cyclic Strain Research Articles

Impact of Cyclic Strain on Elastin Synthesis in a 3D Human Myometrial Culture Model.

The synthesis and assembly of mature, organized elastic fibers remains a limitation to the clinical use of many engineered tissue replacements. There is a critical need for a more in-depth understanding of elastogenesis regulation for the advancement of methods to induce and guide production of elastic matrix structures in engineered tissues that meet the structural and functional requirements of native tissue. The dramatic increase in elastic fibers through normal pregnancy has led us to explore the potential role of mechanical stretch in combination with pregnancy levels of the steroid hormones 17β-estradiol and progesterone on elastic fiber production by human uterine myometrial smooth muscle cells in a three-dimensional (3D) culture model. Opposed to a single strain regimen, we sought to better understand how the amplitude and frequency parameters of cyclic strain influence elastic fiber production in these myometrial tissue constructs (MTC). Mechanical stretch was applied to MTC at a range of strain amplitudes (5%, 10%, and 15% at 0.5 Hz frequency) and frequencies (0.1 Hz, 0.5 Hz, 1 Hz, and constant 0 Hz at 10% amplitude), with and without pregnancy-level hormones, for 6 days. MTC were assessed for cell proliferation, matrix elastin protein content, and expression of the main elastic fiber genes, tropoelastin (ELN) and fibrillin-1 (FBN1). Significant increases in elastin protein and ELN and FBN1 mRNA were produced from samples subjected to a 0.5 Hz, 10% strain regimen, as well as samples stretched at higher amplitude (15%, 0.5 Hz) and higher frequency (1 Hz, 10%); however, no significant effects because of third-trimester mimetic hormone treatment were determined. These results establish that a minimum level of strain is required to stimulate the synthesis of elastic fiber components in our culture model and show this response can be similarly enhanced by increasing either the amplitude or frequency parameter of applied strain. Further, our results demonstrate strain alone is sufficient to stimulate elastic fiber production and suggest hormones may not be a significant factor in regulating elastin synthesis. This 3D culture model will provide a useful tool to further investigate mechanisms underlying pregnancy-induced de novo elastic fiber synthesis and assembly by uterine smooth muscle cells.

Efficient deep reinforcement learning strategies for active flow control based on physics-informed neural networks

Reducing the reliance on intrusive flow probes is a critical task in active flow control based on deep reinforcement learning (DRL). Although a scarcity of flow data captured by probes adversely impacts the control proficiency of the DRL agent, leading to suboptimal flow modulation, minimizing the use of redundant probes significantly reduces the overall implementation costs, making the control strategy more economically viable. In this paper, we propose an active flow control method based on physics-informed DRL. This method integrates a physics-informed neural network into the DRL framework, harnessing the inherent physical characteristics of the flow field using strategically placed probes. We analyze the impact of probe placement, probe quantity, and DRL agent sampling strategies on the fidelity of flow predictions and the efficacy of flow control. Using the wake control of a two-dimensional cylinder flow with a Reynolds number of 100 as a case study, we position a specific number of flow probes within the flow field to gather pertinent information. When benchmarked against traditional DRL techniques, the results are unequivocal: in terms of training efficiency, physics-informed DRL reduces the training cycle by up to 30 rounds. Furthermore, by decreasing the number of flow probes in the flow field from 164 to just 4, the physics-based DRL achieves superior drag reduction through more precise control. Notably, compared to traditional DRL control, the drag reduction effect is enhanced by a significant 6%.

Design Considerations for a Multidisciplinary Approach to Provide Policy Recommendations on Gender-Integrated Recruit Training in the Marine Corps.

Recruit training is designed to transform civilians into physically fit military service members, who embody their service's core values and possess military discipline and skills. At the time this research began, the U.S. Marine Corps (USMC) remained the only service that segregated recruits by gender at the lowest unit level (e.g., platoon) and employed gender-segregated drill instructor teams. USMC's Marine Corps Recruit Depots (MCRD) must comply with a 2020 Congressional Mandate to not segregate training by gender in Parris Island by 2025 and San Diego by 2028. In turn, USMC requested an independent scientific study to analyze current approaches to gender integration at recruit training to propose alternate models and other policy recommendations that increase gender integration while maintaining current USMC standards. The Marine Corps is currently evaluating alternate models and recommendations to optimize entry-level training. This article outlines considerations for choosing the optimal research study design, research methods, and types of data collected in a study intended to provide policy recommendations on gender-integrated recruit training for the USMC. Research data were collected during visits to the MCRDs and selected recruit training locations for the Army, Air Force, Navy, and Coast Guard. Data collection on USMC recruits from three cohorts involved social science assessments (focus groups and surveys) and human performance testing (countermovement jumps and isometric mid-thigh pulls, sleep and activity wearables, and cortisol data) at multiple points in the training cycle. Data on recruits from the sister services were limited to social science assessments. Approximately 600 recruits between the two MCRDs and 160 recruits from the sister services participated in the study during a 7-month timeframe in 2021. The research team conducted extensive ethnographic observations of recruit training at all selected research sites and interviewed training cadre, drill instructors, and service leadership responsible for recruit training (∼90 interviews). Additionally, the research team interviewed 20 experts on gender integration or recruit training who possessed alternate viewpoints from the current USMC practice. The mixed methods study was designed to assess the current gender integration practices at recruit training across the services to generate alternative models of gender integration for USMC. The research team developed a set of multidisciplinary objectives and research questions serving as the foundation of the research study design and data collection process. The study was designed to collect qualitative, quantitative, and administrative data informed by social science and human performance disciplines. To ensure that all aspects and implications relevant to gender integration were considered, select data were collected across services and with stakeholders at all levels. This multidisciplinary research approach provided a comprehensive picture of the current USMC recruit training models. The research team captured multiple perspectives and data points for analysis through an expansive view on gender integration across all services, by interacting with participants at all levels of the institutions in varied ways. The information and data gathered enabled the research team to establish objective, data-driven alternate models, and recommendations for enhancing gender integration at recruit training for the USMC.

A Sex Comparison of the Physical and Physiological Demands of United States Marine Corps Recruit Training.

The U.S. Marine Corps (USMC) recruit training is a 13-week preparatory period for military service men and women. Differences in absolute performance capabilities between sexes may impact physical and physiological responses to the demands of recruit training. The purpose of this study was to monitor U.S. Marine Corps recruits throughout recruit training to comparatively assess workload, sleep, stress, and performance responses in men and women. A total of 281 recruits (men = 182 and women = 99; age = 19 ± 2 years) were monitored and tested. Workload, sleep, and stress assessments occurred at week 2, week 7/8, and week 11 of training. Workload (energy expenditure per kg body mass [EEREL], distance [DIS], steps) and sleep (continuity and duration) were tracked over 72-hour periods using wearable accelerometry and heart rate technology. Stress responses were determined through salivary cortisol analyses. Performance testing, consisting of countermovement vertical jump (CMJ) and isometric mid-thigh pull (IMTP) performance relative to body mass, occurred at weeks 2 and 11. Linear mixed models were used to test for sex, time, and sex-by-time interactions (α < .05). On average, recruits covered 13.0 ± 2.7 km/day, expended 3,762 ± 765 calories/day, and slept 6.2 ± 1.1 hours/night. Sex-by-time interactions were found for DIS, steps, sleep duration, cortisol, and CMJREL performance (P < .05). Planned contrasts revealed that men covered more DIS than women at week 7/8 (P < .001). Women experienced greater step counts compared to men at week 11 (P = .004). Women experienced no significant change in sleep duration (P > .05), whereas men increased sleep duration from week 2 to week 7/8 (P = .03). Women experienced greater sleep duration at week 2 (P = .03) and week 11 (P = .02) compared to men. Women exhibited higher cortisol levels than men at week 2 (P < .001) and week 11 (P < .001). Women experienced declines in cortisol at week 7 compared to week 2 (P < .001). Men experienced no changes in cortisol response at any timepoint (P > .05). Both sexes experienced declines in CMJREL from week 2 to week 11 (P > .001). Sex main effects were observed for EEREL, DIS, CMJREL, and IMTPREL (P < .05) with men experiencing greater overall workloads and producing greater strength and power metrics. Sex main effects were also found for sleep continuity and cortisol (P < .05), for which men experienced lower values compared to women. Time main effects were observed for EEREL, DIS, steps, cortisol, CMJREL, and IMTPREL (P < .05). This study not only highlights the known sex differences between men and women but also sheds light on the different physical and physiological responses of each sex to military training. Interestingly, the greatest physical demands incurred earlier in the training cycle. Despite declining workloads, the stress response was maintained throughout the training, which may have implications for adaptation and performance. In addition, average sleep duration fell notably below recommendations for optimizing health and recovery. Effectively monitoring the demands and performance outcomes during recruit training is essential for determining individual fitness capabilities, as well as establishing the effectiveness of a training program. Individual performance assessments and adequately periodized workloads may help to optimize recruit training for both men and women.

Recruit Profiles Across the U.S. Armed Forces: Implications for Increasing Gender Integration in Recruit Training.

This article uses recent survey data from a study on gender integration at recruit training across the U.S. Armed Forces to examine service, gender, and training differences in recruit's gender attitudes, beliefs, and cohesion metrics. In addition to providing a descriptive understanding of our recruit sample, this article examines factors germane to gender integration at recruit training such as gender attitudes, sexist beliefs, and unit cohesion. This article uses data from a survey of U.S. Marine Corps (USMC), U.S. Army, U.S. Air Force, U.S. Navy, and U.S. Coast Guard recruits as part of a USMC-commissioned study to develop alternate models and recommendations for increasing gender integration in USMC recruit training. At all sites but the USMC, recruits completed the survey 1 to 2 weeks before graduation. At the Marine Corps Recruit Depots, recruits participated in the survey at the beginning (week 2) and end (week 11) of their 13-week training cycle. A 19-question survey captured sociodemographic information, perspectives and experiences during recruit training, and gender attitudes and beliefs. Analyses were conducted on the sample with complete data on the measures reported, including those USMC recruits observed at both week 2 and week 11 (n = 629). Descriptive statistics (percentage) were calculated for all categorical outcome variables. Outcome variables (cohesion, gender, and sexism attitudes) were compared between groups using chi-squared tests or Fisher's exact tests, as appropriate. Recruits differed significantly across services in perceptions of unit cohesion on 3 of the 4 indicators: Platoon members are cooperative, know they can depend on each other, and really respect each other (P < .05). These differences appear to be driven by female recruit difference across service. They reported significant differences in gender role attitudes across the services, with USMC recruits being most likely to endorse traditional gender roles compared to other service recruits. Male USMC recruits were significantly more likely than their female peers to agree that men should achieve outside the home and women should take care of the home and family (21.3% versus 6.3%, P < .05). The only statistical differences in attitudes about equality of treatment and opportunity among recruits were between male and female USMC recruits. All items capturing sexist attitudes elicited statistically significant differences between male USMC recruits and male recruits in other services (P < .05). There were significant differences in all sexism measures between male and female USMC recruits (P < .05). There is a significant decline in some sexist attitudes between weeks 2 and 11 of training among male USMC recruits (P < .05). Nearly one-half to three-fourths of male USMC recruits hold sexist attitudes, even near the end of recruit training. The disparate gender and sexism attitudes of male USMC recruits compared with their peers in other services, and their fellow female USMC recruits suggest efforts to increase gender integration at entry-level training are needed, but also may be challenging since male USMC recruits report the highest levels of sexist attitudes among all recruits.

Strain sensor on a chip for quantifying the magnitudes of tensile stress on cells

During cardiac development, mechanotransduction from the in vivo microenvironment modulates cardiomyocyte growth in terms of the number, area, and arrangement heterogeneity. However, the response of cells to different degrees of mechanical stimuli is unclear. Organ-on-a-chip, as a platform for investigating mechanical stress stimuli in cellular mimicry of the in vivo microenvironment, is limited by the lack of ability to accurately quantify externally induced stimuli. However, previous technology lacks the integration of external stimuli and feedback sensors in microfluidic platforms to obtain and apply precise amounts of external stimuli. Here, we designed a cell stretching platform with an in-situ sensor. The in-situ liquid metal sensors can accurately measure the mechanical stimulation caused by the deformation of the vacuum cavity exerted on cells. The platform was applied to human cardiomyocytes (AC16) under cyclic strain (5%, 10%, 15%, 20 and 25%), and we found that cyclic strain promoted cell growth induced the arrangement of cells on the membrane to gradually unify, and stabilized the cells at 15% amplitude, which was even more effective after 3 days of culture. The platform’s precise control and measurement of mechanical forces can be used to establish more accurate in vitro microenvironmental models for disease modeling and therapeutic research.

Changes in Plasma Free Amino Acid Profile in Endurance Athletes over a 9-Month Training Cycle.

We aimed to evaluate long-term changes in proteinogenic and non-proteinogenic plasma free amino acids (PFAA). Eleven male endurance triathletes participated in a 9-month study. Blood was collected at rest, immediately after exhaustive exercise, and during 30-min recovery, in four consecutive training phases: transition, general, specific, and competition. Twenty proteinogenic and 22 non-proteinogenic PFAAs were assayed using the LC-ESI-MS/MS technique. The structured training modified the patterns of exercise-induced PFAA response, with the competition phase being the most distinct from the others. Branched-chain amino acids (p = 0.002; η2 = 0.216), phenylalanine (p = 0.015; η2 = 0.153), methionine (p = 0.002; η2 = 0.206), and lysine (p = 0.006; η2 = 0.196) declined more rapidly between rest and exhaustion in the competition phase. Glutamine (p = 0.008; η2 = 0.255), glutamate (p = 0.006; η2 = 0.265), tyrosine (p = 0.001; η2 = 0.195), cystine (p = 0.042; η2 = 0.183), and serine (p < 0.001; η2 = 0.346) levels were reduced in the competition phase. Arginine (p = 0.046; η2 = 0.138) and aspartate (p = 0.011; η2 = 0.171) levels were highest during exercise in the transition phase. During the competition phase, α-aminoadipic acid (p = 0.023; η2 = 0.145), β-aminoisobutyric acid (p = 0.007; η2 = 0.167), β-alanine (p < 0.001; η2 = 0.473), and sarcosine (p = 0.017; η2 = 0.150) levels increased, whereas phosphoethanolamine (p = 0.037; η2 = 0.189) and taurine (p = 0.008; η2 = 0.251) concentrations decreased. Overtraining indicators were not elevated. The altered PFAA profile suggests adaptations within energy metabolic pathways such as the tricarboxylic acid cycle, oxidative phosphorylation, ammonia neutralization, the purine nucleotide cycle, and buffering of intracellular H+ ions. The changes seem to reflect normal adaptations.

Out-of-phase thermomechanical fatigue of a single crystal Ni-base superalloy

The present work studies the out-of-phase thermomechanical fatigue (OP-TMF) behavior of the precisely oriented [001] single crystal Ni-base superalloy ERBO/1 (CMSX-4 type). The OP-TMF tests are performed between minimum and maximum temperatures of 1023 and 1223 K and a cyclic mechanical strain amplitude of ±0.5 %. In accordance with previous findings, the present study confirmed that isothermal low cycle fatigue (LCF) tests at 1023 and 1223 K show significantly longer fatigue lives than the OP-TMF tests, specimens with rougher surfaces fail earlier than polished specimens and deformation bands and cracks form in the specimen surface. Two new results were obtained: First, when comparing OP-TMF cycles with fast cooling (tensile part of cycle) and fast vs. slow heating (compressive part of cycle) one finds that the slower heating/compressive cycle is more damaging. Second, analytical scanning electron microscopy of the flanks of an OP-TMF crack allows to study the diffusion-controlled growth kinetics of the oxide and the underlying zone, which is depleted by the oxide forming elements. These results are discussed in the light of the microstructural, mechanical and chemical results of the present study considering previous work on OP-TMF of superalloy single crystals (SXs).

High-cycle and low-cycle fatigue characteristics of multilayered dissimilar titanium alloys

Multilayered structures of dissimilar titanium alloys can achieve excellent fracture ductility and strength, while their fatigue characteristics especially dislocation networks and twin formation are rarely reported. Heterogeneous microstructures are observed in the multilayered TC4/TB8 alloys, including fine acicular α grains, continuous α layer at prior β grain boundaries (αGB) and β matrix on the TB8 layer, together with equiaxed α grains on the TC4 layer. High-cycle fatigue (HCF) and low-cycle fatigue (LCF) tests show that the initial fatigue damage appears at the αGB/β matrix interfaces on the TB8 layer instead of the boned TC4/TB8 interfaces. Since the stress concentration induced by dislocation pile-up is prone to micro-void formation and crack propagation at the αGB/β interfaces. For LCF, the αGB/β interfaces can not only act as impenetrable barriers and sources of lattice dislocations, but also allow the dislocations cross boundaries during cyclic tension and compression because of the high boundary energy. The formation characteristic of deformation twins that is beneficial for the plastic deformation of α grains in TC4 layer during cyclic strain is investigated. Furthermore, the hexagonal dislocation networks are also found within the equiaxed α grains of TC4 layer after LCF, and the role between interface barrier and slip direction in the formation mechanism is analyzed.

Sex Differences and Role of Lysyl Oxidase Like 2 (LOXL2) in Angiotensin II-Induced Hypertension in Mice.

Hypertension, a disease with known sexual dimorphism, accelerates aging associated arterial stiffening, in part due to the activation of matrix remodeling caused by increased biomechanical load. In this study, we tested the effect of biological sex and the role of the matrix remodeling enzyme lysyl oxidase like 2 (LOXL2) in hypertension induced arterial stiffening. Angiotensin II (Ang II) was delivered using osmotic pumps in Loxl2+/- and WT male and female mice. Blood pressure and pulse wave velocity (PWV) were measured noninvasively to assess hypertension and aortic stiffness. Wire myography and uniaxial tensile testing were used to test aortic vasoreactivity and mechanical properties. Aortic wall composition was examined by histology and Western blotting. The effect of biomechanical strain on LOXL2 expression and secretion by vascular smooth muscle (VSMC) and endothelial cells (EC) was evaluated by uniaxial cyclic stretching of cultured cells. The role of LOXL2 catalytic function on VSMC alignment in response to mechanical loading was determined with LOXL2 inhibition and knockout. Ang II infusion induced hypertension in WT and Loxl2+/- mice of both sexes and increased PWV in WT males but not in Loxl2+/- males, WT females, or Loxl2+/- females. LOXL2 depletion protected males from Ang II mediated potentiation of vasoconstriction but worsened in females and improved aortic mechanical properties in both sexes. Histological analysis showed increased aortic wall thickness in hypertensive WT males but not females and increased intralamellar distance in both sexes, that was ameliorated in Loxl2+/- mice. Western blotting revealed increased collagen I, decreased collagen IV, and increased LOXL2 accumulation and processing in hypertensive mice. Hypertensive cyclic strain contributed to LOXL2 upregulation in the cell-derived matrix in VSMCs but not ECs. LOXL2 catalytic function facilitated VSMC alignment in response to biomechanical strain. In males, arterial stiffening in hypertension is driven both by VSMC response and matrix remodeling. Females exhibit a delayed onset of Ang II-induced hypertension with minimal ECM remodeling but with VSMC dysfunction. LOXL2 depletion ameliorates arterial stiffening and preserves functional contractility and aortic structure in male hypertensive mice. LOXL2 depletion improves aortic mechanics but worsens aortic contractility in hypertensive females. VSMCs are the primary source of LOXL2 in the aorta and hypertension increases LOXL2 processing and shifts to collagen I accumulation. Overall, LOXL2 depletion offers protection in young hypertensive males and females.

Bioreactor development for skeletal muscle hypertrophy and atrophy by manipulating uniaxial cyclic strain: proof of concept

Skeletal muscles overcome terrestrial, gravitational loading by producing tensile forces that produce movement through joint rotation. Conversely, the microgravity of spaceflight reduces tensile loads in working skeletal muscles, causing an adaptive muscle atrophy. Unfortunately, the design of stable, physiological bioreactors to model skeletal muscle tensile loading during spaceflight experiments remains challenging. Here, we tested a bioreactor that uses initiation and cessation of cyclic, tensile strain to induce hypertrophy and atrophy, respectively, in murine lineage (C2C12) skeletal muscle myotubes. Uniaxial cyclic stretch of myotubes was conducted using a StrexCell® (STB-1400) stepper motor system (0.75 Hz, 12% strain, 60 min day^-1). Myotube groups were assigned as follows: (a) quiescent over 2- or (b) 5-day (no stretch), (c) experienced 2-days (2dHY) or (d) 5-days (5dHY) of cyclic stretch, or (e) 2-days of cyclic stretch followed by a 3-day cessation of stretch (3dAT). Using ß-sarcoglycan as a sarcolemmal marker, mean myotube diameter increased significantly following 2dAT (51%) and 5dAT (94%) vs. matched controls. The hypertrophic, anabolic markers talin and Akt phosphorylation (Thr308) were elevated with 2dHY but not in 3dAT myotubes. Inflammatory, catabolic markers IL-1ß, IL6, and NF-kappaB p65 subunit were significantly higher in the 3dAT group vs. all other groups. The ratio of phosphorylated FoxO3a/total FoxO3a was significantly lower in 3dAT than in the 2dHY group, consistent with elevated catabolic signaling during unloading. In summary, we demonstrated proof-of-concept for a spaceflight research bioreactor, using uniaxial cyclic stretch to produce myotube hypertrophy with increased tensile loading, and myotube atrophy with subsequent cessation of stretch.

Achieving high fatigue endurance of nanocrystalline Ni/Ni-W layered composites through thermally and mechanically-induced relaxations

Stabilizing non-equilibrium nanograin boundaries has been shown to enhance the strength of nanocrystalline metals. Nevertheless, a thorough comprehension of how these stable grain boundaries impact the fatigue behavior in nanocrystalline metallic layered composites with heterogeneous interfaces is currently lacking. Here, aiming to develop high-performance small components in microelectromechanical systems, we prepared nanocrystalline Ni/Ni-W layered composites that underwent various degrees of annealing treatment. Our results reveal that the Ni/Ni-W layered composites annealed at 200 °C exhibit significantly higher fatigue strength, surpassing that of the as-prepared composites by 40 % and the Pt-10 at.% Au alloy by 17 % which is one of the best candidates for the microelectromechanical systems switches at present. The enhanced fatigue strength is primarily attributed to the annealing-induced grain boundary relaxation and mechanically-induced structural relaxation. Grain boundary relaxation enhances the strength by improving GB stability, while the mechanically-induced structural relaxation results in the coarsening and expansion of triangular columnar grains towards the Ni-W layer during fatigue loading. As a result, the dispersive strain localized regions triggered by the triangular columnar grains undertook cyclic strain accumulation and weakened localized damage accumulation in Ni layers, and thus further improved the fatigue resistance. The finding of the underlying mechanisms may offer a promising approach for designing high-performance materials for microelectromechanical systems switches operating at elevated temperatures.

Intrinsically stretchable organic photovoltaics by redistributing strain to PEDOT:PSS with enhanced stretchability and interfacial adhesion

Intrinsically stretchable organic photovoltaics have emerged as a prominent candidate for the next-generation wearable power generators regarding their structural design flexibility, omnidirectional stretchability, and in-plane deformability. However, formulating strategies to fabricate intrinsically stretchable organic photovoltaics that exhibit mechanical robustness under both repetitive strain cycles and high tensile strains remains challenging. Herein, we demonstrate high-performance intrinsically stretchable organic photovoltaics with an initial power conversion efficiency of 14.2%, exceptional stretchability (80% of the initial power conversion efficiency maintained at 52% tensile strain), and cyclic mechanical durability (95% of the initial power conversion efficiency retained after 100 strain cycles at 10%). The stretchability is primarily realised by delocalising and redistributing the strain in the active layer to a highly stretchable PEDOT:PSS electrode developed with a straightforward incorporation of ION E, which simultaneously enhances the stretchability of PEDOT:PSS itself and meanwhile reinforces the interfacial adhesion with the polyurethane substrate. Both enhancements are pivotal factors ensuring the excellent mechanical durability of the PEDOT:PSS electrode, which further effectively delays the crack initiation and propagation in the top active layer, and enables the limited performance degradation under high tensile strains and repetitive strain cycles.

Learning general features to bridge the cross-domain gaps in few-shot learning

Few-shot learning (FSL) methods have attracted great attention in recent years. However, under the cross-domain few-shot learning (CDFSL) setting, where domain gaps exist between the source and target domains, many FSL methods face performance degradation. One possible reason is the well-trained model can easily learn discriminative features on the source domain but may fail on target domains. To alleviate this problem, it is desirable to learn a model that can extract useful general features from target tasks regardless of domain gaps during the source domain training. In this paper, we propose a dynamic representation enhancement (DRE) framework that maximizes the model’s representation ability to learn diverse and meaningful features on unknown domains. First, we proposed a stage-wise cycling and warm-up feature activation training strategy to make the model continuously explore useful general features during training, instead of just satisfying with fitting well on the limited training data. Second, we designed a task-adaptive loss function to enhance the learning of diverse features by making the model focus on inter-class discriminative features during training. Experimental analysis shows that the proposed DRE framework effectively extracts informative features across various target domains. On the popular cross-domain BSCD-FSL benchmark, the performance of the DRE framework is competitive with the state-of-the-art methods with an average classification accuracy of 51.01%, 66.71% and 74.13% in the 5-way 1-shot, 5-way 5-shot and 5-way 20-shot settings, respectively. Our code is publicly available at https://github.com/ideal-123/Dynamic-Representation-Enhancement-framework.

Nonlinear viscoelastic mechanical behavior and fatigue hysteresis loops modeling of composite laminates

Fatigue constitutive curve is the presentation of mechanical response and damage mechanism of composites. The hysteresis phenomenon in composites is essentially attributed to the viscoelastic properties of the polymer matrix, causing the strain response to lag behind the stress excitation. Combined with the fatigue damage modes of composites, the contributions of elastic, plastic and viscoelastic strains to the cumulative cyclic strain are analyzed to determine the evolution law of the hysteresis loops with cycling. The hysteresis loops and cyclic hysteresis energy models of composites under tensile-tensile fatigue loads are proposed, and the models are verified by the experimental results of four kinds of laminates under several fatigue loads.

Cyclic cumulative strain of coarse-grained soil under large cyclic stress amplitude

The deformation behavior of coarse-grained soil under large cyclic stresses, such as those induced by strong earthquakes, has received limited attention. This study aims to investigate the cyclic accumulation behaviors and hysteresis loops of coarse-grained soil during drained cyclic triaxial tests, spanning a range from small to large cyclic stresses. The cyclic triaxial tests were primarily conducted under anisotropic consolidation conditions, with an axial-to-radial stress ratio of 2.0, confining pressures ranging from 100 to 500 kPa, and cyclic stresses varying from 0.01 to 1.85 times the confining pressure. Additionally, cyclic triaxial tests under isotropic consolidation conditions and constant mean stress conditions were performed for comparison and validation. The test results reveal that the properties of the hysteresis loops exhibit significant nonlinear behavior as cyclic stress increases, particularly concerning their shape, symmetry, degree of closure, and initial tangent modulus of elasticity. The cumulative axial strain displays three stages: strain increases slightly and gently at small cyclic stress, increases rapidly and substantially at medium cyclic stress, and decreases at large cyclic stress. The delineation of these phases is largely governed by the behaviors observed during the first cycle. Moreover, the cumulative volumetric strain increases monotonically with the increase of cyclic stress, with a more rapid increase at large cyclic stress. This study provides valuable insights into the cyclic deformation and constitutive modeling of coarse-grained soil under significant cyclic stress.

Evaluation of pilocarpine iontophoresis vs. exercise-induced sweat sodium concentrations at different exercise intensities

Differences between passive versus active sweat sodium concentration ([Na+]) may alter sodium replacement strategies. The primary purpose of this pilot trial was to compare pharmacologically induced sweat [Na+] (passive) with exercise-induced sweat [Na+] (active) at three different exercise intensities. Methods: This was an internal validation trial, reviewed by Wayne State University’s IRB (IRB 23-02-5555). WSU’s IRB determined that this retrospective, observational, data collection did not fall within the scope of human research regulations. As such, fifteen endurance cyclists participated in four cycling trials (different days, separated by a week) at three exercise intensities (50, 75, and 100% of functional threshold power/FTP) for 20 minutes. Sweat was collected prior to each trial via pilocarpine iontophoresis (passively induced sweat). Then, a macroduct™ (Wescor Inc, Logan, UT) sweat collector was affixed to the same forearm (at a different location than the passively induced sweat collection) and the athlete cycled at the given intensity to collect exercise-induced sweat. Sweat samples were analysed for sweat [Na+] immediately (Sweat Chek Conductivity Analyzer, Wescor Inc, Logan UT), which was the main outcome variable. One-way ANOVA was utilised to detect differences in passive and active sweat [Na+] levels across different exercise intensities. Statistical significance was set a priori at P &lt; 0.05. Results: Fifteen highly trained cyclists between 19-45 years (10 male, 5 female, weight 69 ± 9 kg, FTP 4.16 ± 0.56 W/kg, passive sweat [Na+] 55.6 ± 18.5 mmol/L [range 30-89 mmol/L]) completed this trial. No statistically significant differences were noted between pre (passive) to post (active) sweat [Na+] levels during the 50% FTP, 75% FTP, or 100% FTP conditions. However, when assessing exercise-induced sweat [Na+] minus passive-induced sweat [Na+], significant differences were noted between the 50% (−12.4 ± 7.6mmol/L), 75% (1.3 ± 6.2mmol/L) and 100% FTP (7.5 ± 6.8 mmol/L) exercise intensity conditions (P &lt; 0.001; R2 = 0.61). Although sweat rate was different between the 50% (0.62 ± 0.21 L/h), 75% (1.26 ± 0.34 L/h), and 100% FTP (1.89 ± 0.50 L/h) conditions; P &lt; 0.001; R2 = 0.68, there were no significant associations between sweat rate versus sweat [Na+] change except for the 100% FTP condition (R2 = 0.29; P = 0.04). Conclusions: Exercise-induced sweat approximates pharmacologically induced sweat at cycling intensities around 75% FTP, with lower sweat [Na+] readings at lower (50% FTP) intensities and higher sweat [Na+] readings at higher (100% FTP) cycling intensities.

Preparation of Gradient Polyurethane and Its Performance for Flexible Sensors.

Flexible sensors are prone to the problems of slow recovery rate and large residual strain in practical use. In this paper, a polyurethane functional composite with a gradient change in elastic modulus is proposed as a flexible sensor to meet the recovery rate and residual strain without affecting the motion. Different hard and soft segment ratios are used to synthesize a gradient polyurethane structure. The conductive percolation threshold was obtained between 45 wt% and 50 wt% of flake silver powder. Both gradient polyurethane and gradient polyurethane composites demonstrated that gradient materials can increase the recovery rate and reduce residual strain. The gradient polyurethane composites had a tensile strength of 3.26 MPa, an elastic modulus of 2.58 MPa, an elongation at break of 245%, a sensitivity coefficient of 1.20 at 0-25% deformation, a sensitivity coefficient of 11.38 at 25-75% deformation, a rate of recovery of 1.95 s at a time, and a resistance to fatigue (over 1000 cycles at a fixed strain of 20% showed a stable electrical response). The sensing performance under different cyclic strain frequencies was also investigated. The process has practical applications in the field of wearable skin motion and health monitoring.

Vitamin D and its effect on the endothelial function of cross-country skiers at various stages of the training cycle

The aim of the study was to assess the level of vitamin D and nitric oxide metabolites, and to search for the relationship between them in cross-country skiers at various stages of the training cycle. During the year, two groups of men (20–26 years old, inhabitants of the Komi Republic) were examined four times: cross-country skiers who are active members of the national teams of the Komi Republic/Russia (n = 11) and office workers (n = 10). It was shown that skiers had higher vitamin D levels duration the year compared to office workers. The fluctuations in the levels of vitamin D, calcium and phosphorus detected in cross-country skiers were seasonal, which could lead to significant changes in the synthesis of nitric oxide and the work of the endothelial system. The greatest number of correlations between the studied metabolites in cross-country skiers was revealed at the beginning and end of the annual training season. A significant imbalance in the levels of vitamin D and nitric oxide metabolites found in them in March may negatively affect the success of performance at important competitions.

Impact of Anaerobic Exercise Integrated Into Regular Training on Experienced Judo Athletes: Running Vs. Repetitive Throws.

Kamandulis, S, Dudėnienė, L, Snieckus, A, Kniubaite, A, Mickevicius, M, Lukonaitiene, I, Venckunas, T, Stasiule, L, and Stasiulis, A. Impact of anaerobic exercise integrated into regular training on experienced judo athletes: running vs. repetitive throws. J Strength Cond Res 38(9): e489-e495, 2024-Anaerobic training in high-level athletes is of considerable interest to practitioners aiming to optimize performance. This study compared the impact of interval anaerobic training (IAT) sessions consisting of either high-intensity running or throwing that were performed twice a week together with regular judo training on the anaerobic and aerobic performance of experienced judo athletes. Employing a repeated-measures, counterbalancing, research design, 12 national team judo athletes (7 women and 5 men; mean age, 20.4 ± 0.95 years; mean judo training experience, 13.4 ± 1.4 years; competitive level, black belt first and second Dan) performed each IAT modality for 6 weeks, for a full training cycle of 12 weeks. Assessments of their anaerobic fitness (Cunningham and Faulkner Anaerobic Treadmill Test), sport-specific anaerobic fitness (Special Judo Fitness Test [SJFT]), and aerobic capacity (maximal incremental treadmill running test) were performed before, after 6 weeks, and after 12 weeks of training. The uphill running performance improved by 13.1% over the 12-week period ( p = 0.047). Simultaneously, there was a 9.0% improvement in the SJFT index and a 6.9% increase in the number of throws ( p = 0.011 and p = 0.017, respectively). Although a trend for throwing drills being more effective than interval sprint running was observed, the interaction effect lacked statistical significance ( p = 0.074). Moreover, no substantial changes were noted in aerobic endurance markers. In conclusion, this study suggests that incorporating specific and nonspecific high-intensity drills into a routine training regimen may enhance anaerobic capacity among well-trained judo athletes, potentially leading to favorable competitive outcomes.