Oxidative Cyclization Research Articles

Comprehensive study of the effect of Hf and Ta co-doped MCrAlY bond coat on the high-temperature properties of thermal barrier coating

The MCrAlY bond coat is modified by reactive element (RE) Hf and refractory element Ta, and the high-temperature oxidation resistance of the thermal barrier coating (TBC) before and after the modification are investigated by comparative experiments. The experiments use high-velocity oxygen-fuel (HVOF) to prepare NiCoCrAlY and NiCoCrAlYHfTa bond coats on the surface of GH4099 high-temperature alloy, and then yttrium stabilized zirconia (YSZ) ceramic layers are prepared on the surface of the bond coat by atmospheric plasma spraying (APS), and cyclic oxidation is carried out in air at 1050 °C until failure. It is shown that the oxidation lifetime of the modified TBC reached 1992 h, which is one time higher than that of the unmodified TBC. The doping of Hf and Ta elements reduces the growth rate of the oxide layer, and these diffuse externally and combine with the internally diffused O to form HfO2 and Ta2O5 in the thermally grown oxide (TGO) layer, thereby improving the adhesion and mechanical properties of the oxide layer. In addition, the bond strength and thermal shock resistance of the TBCs are significantly improved after doping with Hf and Ta elements, which prolongs the lifetimes of the TBCs. In this paper, the morphology, structure, and properties of the modified TBCs are characterized to investigate the mechanism of the oxidation lifetime extension.

Sustainable recovery of monomers from PET and PC waste via Thermocatalytic depolymerization for synthesis of polycarbonates and co-polycarbonates

The increasing global demand for sustainable plastics recycling methods has propelled the research and development of innovative depolymerization processes. The Thermocatalytic depolymerization (TCDP) of polyethylene terephthalate (PET) and polycarbonate (PC) has been performed at 200 ℃. Ethylene glycol (EG) is used as a reactant and as well a polymer bond-cleaving agent and Yttrium Oxide (Y2O3) is used as an effective catalyst. Y2O3 acted as a dual role during depolymerization reaction, which converts EG into cyclic ethylene oxide (CEO) and as well as to break the ester and carbonate bonds in the polymer structure of PET and PC respectively. The TCDP reaction conditions were optimized and the maximum quantity of Bis(2-hydroxyethyl) terephthalate (BHET) (80%) and (Bisphenol A (BPA)) (83%) has been recovered by TCDP reaction of PET and PC respectively. The polycarbonate and co-polycarbonate (Co-PC) were synthesized using recovered BPA and BHET with triphosgene via polycondensation reaction. The structure of monomers and polymers was confirmed by existing analytical (FTIR, NMR, XRD and GPC) techniques. The thermal properties of PC and Co-PC were performed by TG analysis. The TGA results reveal that the degradation temperature (Td) of PC and Co-PC are observed at 420.5 and 442.8 °C respectively, which shows that the Td of Co-PC has been increased by 22 ℃ than PC. The TCDP process is an effective method to recover specific value-added products from waste plastics, which could be made into a new versatile material for different applications. This research finding will provide a new direction to convert plastic waste into useful chemicals, which could reduce waste plastic and their carbon footprint and contribute to a more sustainable future by reusing plastic materials and achieving a circular economy.

A Novel Tryptanthrin Derivative D6 Induces Apoptosis and DNA Damage in Non-small-cell Lung Cancer Cells Through Regulating the EGFR Pathway.

Non-small-cell lung cancer is a prevalent malignancy associated with significant morbidity and mortality rates. Tryptanthrin and its derivatives have exhibited potent antitumor activity. This study aims to investigate the inhibitory effect of a novel synthesized tryptanthrin derivative D6 on proliferation and the possible mechanism of human non-small cell lung cancer cell lines (A549) in vitro. In this study, MTT assay, cell migration, colony formation assay, cell cycle analysis, cell apoptosis, JC- 1 staining assay, reactive oxygen species analysis, proteomics, western blotting, high content screening and absorption titrations analysis were performed. We found that D6 inhibited both the proliferation and migration, induced cell cycle arrest in the G2/M phase, increased levels of ROS, decreased mitochondrial membrane potential, and promoted apoptosis in A549 cells. Further mechanistic studies found that D6 reduced EGFR expression in A549 cells and inhibited the EGFR pathway by decreasing phosphorylation levels of EGFR, Stat3, AKT and Erk1/2. Moreover, DNA damage induced by D6 involved an increase in p53/MDM2 ratio and concentration-dependent accumulation of micronuclei. D6 demonstrated significant antitumor activity against A549 cells by inhibiting the EGFR signaling pathway, inducing DNA damage, and subsequently leading to oxidative stress, apoptosis, and cell cycle arrest. Our findings suggest that D6 exhibits potential as an NSCLC drug, owing to its attributes such as antiproliferative activity and ability to induce apoptosis by attenuating the EGFR-mediated signaling pathway.

Preparation and optical characterization of 3D-TiO2 thin films with brilliant colors

In this paper, three-dimensional titanium dioxide (3D-TiO2) thin films with periodic structure were obtained by periodic pulse anodization of high-purity titanium wafers under specific alternating voltage (AV) using asymmetric sawtooth waveforms. The effect of voltage amplitude and electrolyte concentration on the structural color of the 3D-TiO2 thin films were investigated. It is shown that the nanotubes grown by asymmetric sawtooth wave at a specific AV have a “bamboo” shape, and the cycle length, as well as the inner and outer tube diameters of the nanotubes are closely related to the AV. The structural color of the samples was different at varying voltages and electrolyte concentrations for the same oxidation cycle time. The structural color of films grown under low voltage is analyzed to be related to the scattering and absorption of light on their surfaces. The thickness of the film grown under high voltage satisfies the conditions for light interference. The maximum reflected wavelength is red-shifted and then blue-shifted as the electrolyte concentration decreases. The study shows that the microstructure is the main influence on the color change of 3D-TiO2 thin films.

A configuration sampling study of reaction intermediates constituting catalytic cycles for CO oxidation with Pt1/TiO2

A configuration sampling study of reaction intermediates constituting catalytic cycles for CO oxidation with Pt1/TiO2

An Electrosynthesis of 1,3,4-Oxadiazoles from N-Acyl Hydrazones.

The 1,3,4-oxadiazole is a widely encountered motif in the areas of pharmaceuticals, materials, and agrochemicals. This work has established a mild, mediated electrochemical synthesis of 2,5-disubstituted 1,3,4-oxadiazoles from N-acyl hydrazones. Using DABCO as the optimal redox mediator has enabled a mild oxidative cyclisation, without recourse to stoichiometric oxidants. In contrast to previous methods, this indirect electrochemical oxidation has enabled a broad range of substrates to be accessed, with yields of up to 83%, and on gram scale. The simplicity of the method has been further demonstrated by the development of a one-pot procedure, directly transforming readily available aldehydes and hydrazides into valuable heterocycles.

M6A-activated BACH1 exacerbates ferroptosis by epigenetic suppression HSPB1 in severe acute pancreatitis.

Severe acute pancreatitis (SAP) is characterized by acute inflammation of the pancreas. The transcription factor BTB and CNC homology 1 (BACH1) has been implicated in various biological processes, including oxidative stress, apoptosis, and cell cycle regulation. However, its involvement in the pathogenesis of SAP remains relatively understudied. In the present work, our data demonstrated that BACH1 level was significantly increased in SAP patients, cellular, and animal models, while heat shock protein B1 (HSPB1) expression was weakened. Mechanistic assays validated that BACH1 acted as a transcriptional inhibitor of HSPB1. Moreover, HPDE6-C7 cells were stimulated with cerulein (Cer) and LPS to mimic the pathological stages of SAP in vitro. Depletion of BACH1 remarkably improved cell survival and alleviated the oxidative stress, ferroptosis, and inflammatory responses in SAP cell models. However, these changes were dramatically reversed upon co-inhibition of HSPB1. Animal findings confirmed that loss of BACH1 decreased pancreatic injury, inflammatory responses, and ferroptosis, but these effects were weakened by HSPB1 silence. Overall, these findings elucidate that the overexpression of BACH1 favors the ferroptosis and inflammation by transcriptionally inhibiting HSBP1, thereby exacerbating SAP progression.

The oxidation behavior and interfacial reaction between SiO2 coating and Ti45Al8.5Nb alloy

In this study, SiO2 coating was electrodeposited on Ti45Al8.5Nb alloy to enhance its oxidation resistance at 900 °C. The focus was on the interaction between SiO2 coating and the alloy substrate, and specifically, the role of the Nb element in this context. The formation of a SiO2/(Ti, Nb)O2/Ti5Si3 + Al2O3 three-layer oxide scale significantly inhibits the inward diffusion of oxygen. Beyond the characteristic Ti5Si3 + Al2O3 layer at the interface, the Nb2Al phase embedded within Ti5Si3 was observed. Concurrently, the presence of a (Ti, Nb)O2 layer was confirmed. The relationship between the coating thickness and oxidation resistance was also investigated. It was found that the thickness of the SiO2 coating affects the density of the oxide scale and the inward diffusion rate of oxygen. Moreover, due to the good adhesion to the substrate, the derived oxide scale exhibited good anti-peeling performance when subjected to a cyclic oxidation test.

CYP76BK1 orthologs catalyze furan and lactone ring formation in clerodane diterpenoids across the mint family.

The Lamiaceae (mint family) is the largest known source of furanoclerodanes, a subset of clerodane diterpenoids with broad bioactivities including insect antifeedant properties. The Ajugoideae subfamily, in particular, accumulates significant numbers of structurally related furanoclerodanes. The biosynthetic capacity for formation of these diterpenoids is retained across most Lamiaceae subfamilies, including the early-diverging Callicarpoideae which forms a sister clade to the rest of Lamiaceae. VacCYP76BK1, a cytochrome P450 monooxygenase from Vitex agnus-castus, was previously found to catalyze the formation ofthe proposed precursor to furan and lactone-containing labdane diterpenoids. Through transcriptome-guided pathway exploration, we identified orthologs of VacCYP76BK1 in Ajuga reptans and Callicarpa americana. Functional characterization demonstrated that both could catalyze the oxidative cyclization of clerodane backbones to yield a furan ring. Subsequent investigation revealed a total of 10 CYP76BK1 orthologs across six Lamiaceae subfamilies. Through analysis of available chromosome-scale genomes, we identified four CYP76BK1 members as syntelogs within a conserved syntenic block across divergent subfamilies. This suggests an evolutionary lineage that predates the speciation of the Lamiaceae. Functional characterization of the CYP76BK1 orthologs affirmed conservation of function, as all catalyzed furan ring formation. Additionally, some orthologs yielded two novel lactone ring moieties. The presence of the CYP76BK1 orthologs across Lamiaceae subfamilies closely overlaps with the distribution of reported furanoclerodanes. Together, the activities and distribution of the CYP76BK1 orthologs identified here support their central role in furanoclerodane biosynthesis within the Lamiaceae family. Our findings lay the groundwork for biotechnological applications to harness the economic potential of this promising class of compounds.

The Role of Mutated Calreticulin in the Pathogenesis of BCR-ABL1-Negative Myeloproliferative Neoplasms.

Myeloproliferative neoplasms (MPNs) are characterized by increased proliferation of myeloid lineages in the bone marrow. Calreticulin (CALR) 52 bp deletion and CALR 5 bp insertion have been identified in essential thrombocythemia (ET) and primary myelofibrosis (PMF). There is not much data on the crosstalk between mutated CALR and MPN-related signaling pathways, such as JAK/STAT, PI3K/Akt/mTOR, and Hedgehog. Calreticulin, a multifunctional protein, takes part in many cellular processes. Nevertheless, there is little data on how mutated CALR affects the oxidative stress response and oxidative stress-induced DNA damage, apoptosis, and cell cycle progression. We aimed to investigate the role of the CALR 52 bp deletion and 5 bp insertion in the pathogenesis of MPN, including signaling pathway activation and functional analysis in CALR-mutated cells. Our data indicate that the JAK/STAT and PI3K/Akt/mTOR pathways are activated in CALR-mutated cells, and this activation does not necessarily depend on the CALR and MPL interaction. Moreover, it was found that CALR mutations impair calreticulin function, leading to reduced responses to oxidative stress and DNA damage. It was revealed that the accumulation of G2/M-CALR-mutated cells indicates that oxidative stress-induced DNA damage is difficult to repair. Taken together, this study contributes to a deeper understanding of the specific molecular mechanisms underlying CALR-mutated MPNs.

Extending the understanding of Shannon’s safe stimulation limit for platinum electrodes: biphasic charge-balanced pulse trains in unbuffered saline at pH = 1 to pH = 12

Objective.In neural electrical stimulation, safe stimulation guidelines are essential to deliver efficient treatment while avoiding neural damage and electrode degradation. The widely used Shannon's limit,k, gives conditions on the stimulation parameters to avoid neural damage, however, underlying damage mechanisms are not fully understood. Moreover, the translation from bench testing toin vivoexperiments still presents some challenges, including the increased polarisation observed, which may influence charge-injection mechanisms. In this work, we studied the influence on damage mechanisms of two electrolyte parameters that are differentin vivocompared to usual bench tests: solution pH and electrolyte gelation.Approach.The potential of a platinum macroelectrode was monitored in a three-electrode setup during current-controlled biphasic charge-balanced cathodic-first pulse trains. Maximum anodic and cathodic potential excursions during pulse trains were projected on cyclic voltammograms to infer possible electrochemical reactions.Main results.In unbuffered saline of pH ranging from 1 to 12, the maximum anodic potential was systematically located in the oxide formation region, while the cathodic potential was located the molecular oxygen and oxide reduction region whenkapproached Shannon's damage limit, independent of solution pH. The results support the hypothesis that Shannon's limit corresponds to the beginning of platinum dissolution following repeated cycles of platinum oxidation and reduction, for which the cathodic excursion is a key tipping point. Despite similar potential excursions between solution and gel electrolytes, we found a joint influence of pH and gelation on the cathodic potential alone, while we observed no effect on the anodic potential. We hypothesise that gelation creates a positive feedback loop exacerbating the effects of pH ; however, the extent of that influence needs to be examined further.Significance.This work supports the hypothesis of charge injection mechanisms associated with stimulation-induced damage at platinum electrodes. The validity of a major hypothesis explaining stimulation-induced damage was tested and supported on a range of electrolytes representing potential electrode environments, calling for further characterisation of platinum dissolution during electrical stimulation in various testing conditions.

Enhanced cyclic oxidation resistance of Metcoloy II coated ASTM A53 Grade B steel by electric arc thermal spray

This study evaluates the enhanced cyclic oxidation resistance of Metcoloy II coatings applied to ASTM A53 Grade B steel substrates using electric arc thermal spray, across temperatures ranging from 500 °C to 700 °C. The research identifies distinct performance differences between coated and uncoated samples under cyclic oxidation conditions. Initially, both sample types exhibited mass increases due to oxide formation. However, uncoated samples developed non-protective iron oxides, while Metcoloy II coatings formed a protective layer of chromium and nickel oxides. During 500 oxidation cycles at 500 °C, uncoated samples showed a rapid mass increase, indicative of accelerated oxidation, whereas Metcoloy II coatings experienced a controlled mass gain, reflecting effective protection by chromium and aluminum oxides. At 700 °C, the mass variation trends were similar for both types initially; however, uncoated samples suffered significant mass loss due to iron oxide volatilization, emphasizing their susceptibility. Conversely, Metcoloy II coatings exhibited a reduced but steady mass increase, indicating their resilience under high-temperature conditions. Microstructural analysis confirmed the presence of a protective oxide layer in the coated samples, which is essential for reducing substrate oxidation. Although challenges such as coating detachment and thinning were observed, particularly at higher temperatures, Metcoloy II coatings maintained their protective role. This study highlights the coating's effectiveness in extending the lifespan of steel substrates subjected to cyclic oxidation, providing valuable insights for optimizing protective coatings in demanding industrial applications and advancing material science strategies for enhanced durability.

Cobalt-Catalyzed Regio-, Diastereo- and Enantioselective Reductive Coupling of 1,3-Dienes and Aldehydes.

Catalytic regio-, diastereo- and enantioselective reductive coupling of 1,3-dienes and aldehydes through regio- and enantioselective oxidative cyclization followed by regio- and diastereoselective protonation promoted by a chiral phosphine-cobalt complex is presented. Such processes represent an unprecedented reaction pathway for cobalt catalysis that enable selective transformation of the more substituted alkene in 1,3-dienes, affording a broad scope of bishomoallylic alcohols without the need of pre-formation of stoichiometric amounts of sensitive organometallic reagents in up to 98% yield, >98:2 regioselectivity, >98:2 dr and 98:2 er. Application of this method to construction of axial stereogenicity and deuterated stereogenic center provided a wide range of multifunctional chiral building blocks that are otherwise difficult to access. DFT calculations revealed the origin of regio- and stereoselectivity as well as a unique oxidative cyclization mechanism for cobalt catalysis.

Recovery and restoration of glass fibers from end‐of‐life composite waste through pyrolysis and partial oxidation processes combined with hot alkaline surface treatments

AbstractEnvironmental hazards caused by the ever‐increasing end‐of‐life (EoL) glass fiber reinforced polymer (GFRPs) composite waste is of major concern for the sustainable development of the industry. Compared to land filling or incineration, pyrolysis is one of the most promising environmentally friendly methods of disposal of EoL GFRPs. Pyrolysis not only results in recovery of clean glass fibers but other valuable products, such as oils. However, long processing time at elevated temperature leads to aggravation of already existed surface flaws along with the structural changes. Therefore, thermal conditioning of glass fibers results in severe deterioration of the strength in recovered fibers and hence limiting the use of recovered fibers to low end‐products. In this study, a new strategy was adopted where instead of complete cleaning of the fibers at pyrolysis stage, the fibers were partially oxidized and the complete removal of char from the surface of glass was done during hot alkaline etching. This strategy was opted to enhance the quality of the required fibers while reducing the processing time. The results showed ~200% increase in strength of fibers after the combined treatment of pyrolysis and post etching compared to the just pyrolyzed samples with etching time of just 1 min.Highlights End‐of‐life panels of GFRPs were pyrolyzed under inert environment of Argon. Residual char was partially removed through post oxidation under flowing air. Hot alkaline etching resulted in complete removal of char and surface defects. Partial oxidation and short etching cycles resulted in improved strength of recovered glass fibers.

High-temperature oxidation behavior of multi-phase ceramic particles-reinforced TiAl composites with different microstructures

The high-temperature oxidation behaviors of multiphase ceramic particle-reinforced TiAl composites with different microstructures were investigated. The TiAl composites were prepared via spark plasma sintering by introducing 0.5 wt % graphene oxide and 0.3 wt% SiC into Ti-48Al-2Nb-2Cr. The near-γ equiaxed microstructure, dual-phase microstructure, and nearly fully lamellar microstructure were obtained by sintering at 1200, 1250, and 1300 °C, respectively. Moreover, multiphase ceramic particles were uniformly distributed in the TiAl matrix. Cyclic oxidation was conducted at 950 °C in air for 100 h. The TiAl composite with the nearly fully lamellar microstructure exhibited the best oxidation resistance (mass gain of 1.83 mg/cm2) and that with the near-γ microstructure exhibited the worst oxidation resistance (2.47 mg/cm2). The superior oxidation resistance of the TiAl composite with the nearly fully lamellar microstructure is attributed to the uniform distributions of Ti2AlC particles at the α2/γ lamellae interfaces and Ti5Si3 particles at the lamellae colony boundaries, which hinder atomic diffusion. Moreover, the α2/γ lamellae colonies facilitate the formation of Nb-rich and Cr-rich phases at the interface of the oxide layer and substrate, which act as a protective barrier against atomic diffusion.

Flubendiamide provokes oxidative stress, inflammation, miRNAs alteration, and cell cycle deregulation in human prostate epithelial cells: The attenuation impact of synthesized nano-selenium using Trichodermaaureoviride

Flubendiamide (FBD) is a novel diamide insecticide extensively used with potential human health hazards. This research aimed to examine the effects of FBD on PrEC prostate epithelial cells, including Oxidative stress, pro-inflammatory responses, modifications in the expression of oncogenic and suppressor miRNAs and their target proteins, disruption of the cell cycle, and apoptosis. Additionally, the research investigated the potential alleviative effect of T-SeNPs, which are selenium nanoparticles biosynthesized by Trichoderma aureoviride, against the toxicity induced by FBD. Selenium nanoparticles were herein synthesized by Trichoderma aureoviride. The major capping metabolites in synthesized T-SeNPs were Isochiapin B and Quercetin 7,3′,4′-trimethyl ether. T-SeNPs showed a spherical shape and an average size between 57 and 96.6 nm. FBD exposure (12 μM) for 14 days induced oxidative stress and inflammatory responses via overexpression of NF-κB family members. It also distinctly caused upregulation of miR-221, miR-222, and E2F2, escorted by downregulation of miR-17, miR-20a, and P27kip1. FBD encouraged PrEC cells to halt at the G1/S checkpoint. Apoptotic cells were drastically increased in FBD-treated sets. Treatment of T-SeNPs simultaneously with FBD revealed its antioxidant, anti-inflammatory, and antitumor activities in counteracting FBD-induced toxicity. Our findings shed light on the potential FBD toxicity that may account for the neoplastic transformation of epithelial cells in the prostate and the mitigating activity of eco-friendly synthesized T-SeNPs.

Enhancing oxidation resistance via grain boundary engineering in L12-strengthened medium entropy alloys

The concept of grain boundary engineering (GBE) has been successfully applied to L12-strengthened (CoCrNi)94Al3Ti3 medium entropy alloy, with the aim of improving the oxidation resistance by increasing the ratio of special boundaries and suppressing discontinuous precipitation. Surprisingly, our results reveal that GBE treatment not only slows down the oxidation kinetics and but also alters the oxide scale from TiO2 and multi-defect Cr2O3 to continuous and protective Cr2O3 and Al2O3, thereby contributing to an enhanced oxidation and anti-spalling resistance. The GBE treatment reduces the oxidation weight gain of the current alloy from 1.950 mg cm–2 to 1.211 mg cm–2 after 100 h of cyclic oxidation at 800 °C. The findings show that the extensive outward diffusion of Ti accelerates ion transport and promotes microporosity, thus leading to more defects being formed in the oxide film. The GBE treatment suppresses the discontinuous precipitation of the Ti-bearing L12 phase and breaks the random large angular grain boundaries network, inhibiting the diffusion of Ti and ultimately enhancing the oxidation properties of the alloy. The current work provides an idea of oxidation resistance enhancement for Ti-bearing L12-strengthened alloys without changing the alloy composition.

Solvent-free oxidation of alcohols by a heterogeneous Fe3O4@SiO2-DTPA-Ru nanocatalyst

A novel magnetic heterogeneous ruthenium catalyst (Fe3O4@SiO2-DTPA-Ru) was prepared by using magnetic silicon spheres as support, ethylenediaminepentaacetic acid (DTPA) as functional ligands, ruthenium chloride as catalyst precursor. The results obtained after a series of characterizations showed that the catalyst Fe3O4@SiO2-DTPA-Ru was 5–15 nm in size with a 0.205 nm lattice and contained 0.31 mmol Ru/g catalyst. It also had a good thermal stability under 232°C which was verified by thermal filtration method. Besides, the saturation magnetic intensity was as high as 20.54 emu/g. The catalyst Fe3O4@SiO2-DTPA-Ru was applied for the first time into thirteen oxidation reactions of aromatic alcohols and showed good catalytic activity even the yields of eleven aromatic alcohols were all over 90%. In catalyzing 1-phenylethanol to acetophenone, its TOF and TON were 9677 h−1 and 6991, respectively. The Fe3O4@SiO2-DTPA-Ru catalyst could still maintain good reusability and catalytic performance after 15 cycles of 1-phenylethanol oxidation, which was superior to many reported catalysts and had great potential in future industrial production.

Oxidized-Sulfur Decorated Two-Dimensional Cobalt(II) Porphyrin Covalent Organic Framework as a Photocatalyst and Proof-on Action Study in Oxidative Cyclization of Thioamide

Oxidized-Sulfur Decorated Two-Dimensional Cobalt(II) Porphyrin Covalent Organic Framework as a Photocatalyst and Proof-on Action Study in Oxidative Cyclization of Thioamide

Preparation of magnetite nanoparticles and their application in the removal of methylene blue dye from wastewater

Wastewater is discharged in large amounts from different industries; thus, wastewater treatment is currently one of the main concerns, advanced oxidation is a promising technique for wastewater treatment. This research aims to synthesize magnetite nanoparticles and study their application in wastewater treatment via adsorption and advanced oxidation processes. Magnetite nanoparticles were synthesized via coprecipitation technique between ferric and ferrous sulfate at a molar ratio of 2:1. The prepared sample was characterized using FTIR, XRD, TEM, BET surface area, zeta potential, VSM, and UV‒visible spectroscopy. XRD confirmed the formation of a single face-centered cubic (FCC) spinel structure of Fe3O4. TEM revealed an average particle size of 29.2 nm and a BET surface area of 70.1 m2 g−1. UV‒visible spectroscopy revealed that the UV–visible peak of the sample was obtained at 410 nm. VSM confirmed the attraction of the sample to a magnet with a magnetization of 60 (emu/g). The removal efficiency of methylene blue was studied using adsorption and advanced oxidation methods. For adsorption, the studied parameters were dye concentration 2–10 ppm, 3–10 pH, and 50:300 mg Fe3O4/L. For advanced oxidation, peroxide was used with nanomagnetite as a catalyst, and the studied parameters were pH 2–11, magnetite dose 20–200 PPM, and peroxide dose 500–2000 PPM. The removal efficiency by adsorption reached 95.11% by adding 50 mg of Fe3O4/L and 10 ppm dye conc at 6.5 pH; on the other hand, in advanced oxidation, it reached 98.5% by adding 110 PPM magnetite and 2000 ppm H2O2 at pH 11. The magnetite nanoparticles were reused for ten cycles of advanced oxidation, for a 10% reduction in removal efficiency at the tenth cycle.