This article explores the fatigue characteristics of acrylonitrile butadiene styrene (ABS) components fabricated using fused filament fabrication (FFF) additive manufacturing technology. ABS is frequently used as a polymeric thermoplastic material in open-source FFF machines for a variety of engineering applications. However, a comprehensive understanding of the mechanical properties and execution of FFF-processed ABS components is necessary. Currently, there is limited knowledge regarding the fatigue behavior of ABS components manufactured using FFF AM technology. The primary target of this study is to evaluate the results of part-build directions and build orientation angles on the tensile fatigue behavior exhibited by ABS material. To obtain this target, an empirical investigation was carried out to assess the influence of building angles and orientation on the fatigue characteristics of ABS components produced using FFF. The test samples were printed in three distinct directions, including Upright, On Edge, and Flat, and with varying orientation angles ([0°, 90°], [15°, 75°], [30°, 60°], [45°]), using a 50% filling density. The empirical data suggest that, at each printing angle, the On-Edge building orientation sample exhibited the most prolonged vibrational duration before fracturing. In this investigation, we found that the On-Edge printing direction significantly outperformed the other orientations in fatigue life under cyclic loading with 1592 loading cycles when printed with an orientation angle of 15°-75°. The number of loading cycles was 290 and 39 when printed with the same orientation angle for the Flat and Upright printing directions, respectively. This result underscores the importance of orientation in the mechanical performance of FFF-manufactured ABS materials. These findings enhance our comprehension of the influence exerted by building orientation and building angles on the fatigue properties of FFF-produced test samples. Moreover, the research outcomes supply informative perspectives on the selection of building direction and building orientation angles for the design of 3D-printed thermoplastic components intended for fatigue cyclic-loading applications.