Cyclea Peltata Research Articles

Mass Spectrometry-based Alkaloid Profiling of Cyclea peltata Roots and Evaluation of In vitro Cytotoxic Activity

Background: Cyclea peltata (Lam.) Hook. f. and Thoms., an ethnomedicinal herb with antitoxin and anti-herpes activities, is used to cure smallpox, wounds, diabetic disorders of the skin, and its use as a cooling agent. In the present study, an attempt has been made to screen different alkaloids present in Cyclea peltata roots using LC-ESI-MS/MS and to evaluate the in vitro cytotoxic activity of alkaloids. Methods: The detection of alkaloids in C. peltata root extract was carried out by performing MRM-based assay using liquid chromatography-mass spectrometry. The cytotoxic effect of the acetone crystalized fraction was performed against human pancreatic cancer using MIA PaCa-2 cells. Results: The study revealed the presence of tetrandrine and fangchinoline as the major BBI alkaloids in the roots of this herb, along with cycleacurine, isochondrodendrine, cycleahomine and tetrandrine mono-N-2’-oxide. Furthermore, the present study also detected two precursors of BBI alkaloids such as d-Coclaurine, (-)-N-methylcoclaurine. The study also showed a significant (p<0.05) cytotoxic effect of the acetone crystalized fraction with an IC50 value of 59.85 μg/ml. Conclusion: C. peltata root is the major source of tetrandrine and fangchinoline with potent cytotoxic effect.

Anthelmintic Activity of Methanolic Extract of Leaves Of Cyclea Peltata Lam and it’s Statistical Analysis

The aim of this research is to develop a safe and effective anthelmintic from plants, due to the resistance and toxic effect of synthetic anthelmintics on animals and humans. There are several side effects for synthetic drugs including drug resistance, toxicity, and drug residual in animal products. So there is a need for the exploration of medicinal plants for the treatment of different types of worm infections.
 In this research a medicinally and traditionally important plant is used, leaves of Cyclea peltata Lam. The methanolic extract of the leaves were prepared by maceration technique and are monitored for the anti-helminthic activity against adult Pheretima posthuma worms. The reason for selecting this plant is due to the presence of tannins, which have been proved by previous phytochemical studies. Tannins have the capability to bind free proteins in the cuticle, oral cavity of helminths, thus causing the death. Albendazole (25 mg/5ml) is used as a controlled drug by using the adult motility assay method. Three concentrations were prepared (25, 50, and 100mg/5ml). The time required for the immobility and death of the worms were determined. This study deals with the statistical analysis by one way ANOVA followed by Tukey’s multiple comparison test at 95% confidence interval using graph pad prism software version 9.0.0 reveals the result that the methanolic extract of the Leaves of Cyclea peltata Lam causes the immobility and death of the vermicular in a concentration-dependent manner as compared to the standard drug Albendazole.

Exploration of Phaeanthine: A Bisbenzylisoquinoline Alkaloid Induces Anticancer Effect in Cervical Cancer Cells Involving Mitochondria-Mediated Apoptosis.

Natural-product-based pharmacophores possess considerably more structural diversity, attractive physicochemical features, and relatively less toxicity than synthesized drug entities. In this context, our studies on phaeanthine, a bisbenzylisoquinoline alkaloid isolated from the rhizomes of Cyclea peltata (Lam) Hook.f & Thoms., showed selective cytotoxicity toward cervical cancer cells (HeLa) with an IC50 of 8.11 ± 0.04 μM. Subsequent investigation with in silico molecular docking of phaeanthine displayed preferential binding to the antiapoptotic protein Akt as reflected by a docking score of -5.023. Interestingly, the follow-up in vitro assessment of the compound correlated with mitochondria-mediated apoptosis specifically by downregulating the expression of Akt and p-Akt, including other antiapoptotic proteins MCl-1, IGF-2, and XIAP. In the complementary in vitro assessment, mitochondrial membrane polarization and dynamics of intercellular cytochrome c validated the intrinsic mechanism of the apoptotic phenomenon. To the best of our knowledge, this is the first comprehensive anticancer profiling study of phaeanthine against HeLa cells.

Onsite detection of cadmium and nickel using gold nanospheres and nanotriangles capped with Cyclea peltata phytochemicals

Small-sized gold nanospheres and nanotriangles (CPAuNPs) were synthesized to visually identify low concentrations of cadmium and nickel (20 ppm) in polluted water samples. Three factors i.e Cyclea peltata extract concentration (X1), temperature (X2), pH (X3) and agitation speed (X4) were optimized for maximum CPAuNPs synthesis by central composite design. The optimized conditions were found to be CP extract concentration of 24.20%, pH of 7.68 and agitation speed of 43 RPM at which maximum yield of 0.412 g/L was obtained with a fold increase of 1.42 as compared to unoptimized conditions. Gold nanospheres were in the size range of 25–36 nm and nano triangles of 100–140 nm with zetapotential of −31.8 mV. The synthesized CPAuNPs demonstrated good selectivity for visual detection of cadmium and nickel at ppm levels, where the colour of CPAuNPs changed from pink to purple. Upon interaction of CPAuNPs with Cd+3 and Ni+2 in the varied concentration ranges, a maximum red shift was seen at 20 ppm, which confirmed agglomeration. The variation in absorbance ratio (A640nm/A523nm) for Cd+3 and (A610nm,/A523nm) for Ni+2 showed linearity upto 50 ppm with respect to increased concentration in both the cases with R2 = 0.926 in case of Cd+3 and R2 = 0.950 for Ni+2. Hence, a portable low-cost detection kit for specific heavy metals could be constructed, employing this plant reduced nanospheres, for on-site detection sensing of cadmium and nickel in industrial effluents upto 20 ppm limits.

Use of Cyclea peltata mediated gold nanospheres for adsorptive degradation of methyl green dye

In this study, gold nanospheres were synthesized due to catalytic reduction of gold ions by phytochemicals of Cyclea peltata leaf extract (CPAuNPs) in 5 h and subsequently used for removal of methyl green (MG) dye. CPAuNPs were characterized by UV–Visible spectrophotometry, scanning electron microscopy (SEM), energy-dispersive X ray spectroscopy (EDAX), Fourier Transformed Infrared Spectroscopy (FT-IR), X-Ray Diffraction (XRD) and BET analysis. CPAuNPs were spherical, of size 25.73–41.69 nm with SPR peak at 543 nm. CPAuNPs were crystalline in nature and could adsorb 86.02 ± 0.12 % of 50 mg/L MG post one factor at a time analysis. Adsorption followed pseudo-second-order kinetics (k1 = 4.43E−03 g/mg.h), was observed as multilayer chemisorption as the equilibrium data best fitted Freundlich isotherm (R2 = 0.856). Upon interaction of MG by CPAuNPs, hydroxylation of MG lead to formation of crystal violet by attack of N-ethyl N N-Dimethyl ammonium group by hydroxide ions, as evident from LCMS chromatograms.

Phytopharmacological characterization of different extracts and fractions of Cyclea peltata

Cyclea peltata is a shrub belongs to Menispermiaceae family and is commonly known as “Pedal” or “Pada thalli”. The plant has been used since ancient times in traditional medicine as a healing herb for the treatment of various ailments such as skin infections, wounds, antipyretic, diuretic, diabetes, asthma, jaundice and snakebite poisoning. The objective of the present study was to characterize the active phytochemical constituents present in the aqueous, methanolic and n-hexane, dichloromethane, nbutanol, water fractions of methanolic extracts of C. peltata. To analyse the potent bioactive compounds, present in the extracts and fractions, Fourier Transform Infrared Spectroscopy was used. The qualitative phytochemical screening unveiled the presence of steroids, alkaloids, glycosides, flavonoids, terpenoids, saponins in aqueous extract and alkaloids, phenols, tannins, flavonoids, terpenoids, saponins in methanolic extract. The fractions of C. peltata showed the presence of alkaloids, phenols, tannins, flavonoids, terpenoids, saponins. The Infrared Spectroscopy profiling showed the presence of alcohols, phenols, amines, carboxylic acids, aldehydes, ketones, alkenes, alkanes and aromatic compounds. From the study, it is concluded that the bioactive compounds present in the extracts and fractions of C. peltata was alkaloids, phenols, flavonoids and terpenoids which is responsible for the diverse pharmacological properties.

Development of EPS-rich herbal shampoo base fermented using Cyclea peltata leaf powder and Lactobacillus plantarum.

Studies show that the extensive use of chemical shampoos has a negative impact on health. Given the recent trends, the use of herbal shampoos is gaining importance. Cyclea peltata is a common plant in Kerala's coastal region that has traditionally been used to aid in wound healing, allergy relief, and hair and scalp improvement. Greenly produced silver nanoparticles made from plant sources have a wide range of medical applications. The main objective is to optimize the base of the shampoo with better solid content by OFAT studies and characterize the silver nanoparticles synthesized using post optimized shampoo base. Cyclea peltata leaves were fermented with Lactobacillus plantarum for the shampoo formulation, substituting the chemical basis gelatin with an herbal fermented foundation. Silver nanoparticles combined with an herbal formulation should be as safe as herbal shampoo while also being as effective as chemical shampoo. Variation of concentration of Cyclea peltata leaf powder, variation of sucrose concentration, variation of concentration of yeast extract, variation of L.plantarum inoculum, variation of temperature, variation of agitation speed, and variation of time were all studied using the OFAT (One Factor At a Time) method. Silver nanoparticles synthesized using post optimized shampoo bases were also characterized by particle size, zeta potential, and FTIR analysis in order to better understand their properties. The results clearly indicated that all the six factors had a significant effect on the growth and production of EPS. The pH considered for the shampoo base is above 5so as to maintain the acidic mantle of the scalp. Green synthesized silver nanoparticles from post optimized shampoo base were obtained within 17th hour of incubation, with single surface plasmon resonance at 420nm. Nanoparticles showed a peak at -11.6mv of zeta potential which means that the particles are less agglomerative and stable. Similar groups were seen in ftir spectrum of fermented silver nanoparticles and the plant extract which confirmed the capping of nanoparticles with plant phytochemicals. The study successfully prepared and characterized green synthesized silver nanoparticles from post optimized shampoo base and also optimized the shampoo base based on the EPS production. Characterization of the silver nanoparticles found that the nanoparticles synthesized were stable, less agglomerative,and had several useful components present in it.

The Anti-leukemic Potential of Cyclea peltata as Validated by Phytochemical and Cell Line Studies

Hematological malignancies for a very large percentage of cancers occurring the world over and lead to a very large number of deaths each year. It is not just the disease but also the currently available cure which are equally responsible for the side effects and discomfort experienced by the patients. The side effects of the current chemotherapeutic agents' available range from mouth ulcers, diarrhea, temporary hair loss, rashes, nausea, vomiting, and fatigue to low blood cell counts, increased risk of infections, graft versus host disease, tumor lysis syndrome, differentiation syndrome, and difficulty in conceiving. Due to these myriad side effects researchers and on a continuous lookout for newer treatment alternatives. For most developing countries one of the most attractive options is offered by herbals or plant-based medicines. One such herb is Cyclea peltata. It is a climbing shrub found aplenty in southern and eastern India. It has been used traditionally to cure lots of minor ailments. This paper explores the possibility of using the root extract for the treatment of leukemia. To do this, the extracts of the roots were prepared in different organic solvents to ensure complete extraction of all phytoconstituents fractions. The extracts were then tested on 3 different human cell lines. The potential cause of the antileukemic potential of the plant was also hypothesized and proven by carrying out antioxidant studies using the DPPH free radical scavenging assay. The probable active constituents were identified using the liquid chromatography-mass spectrometry assessment, wherein the responsible fractions were successfully identified. The studies showed that the alkaloid and phenolic phytoconstituents were responsible for the antileukemic potential via their antioxidant activity. This lays down the groundwork of offering a new and safer treatment option that could be further explored to be made into one having actual clinical outreach.

Organoleptic and physicochemical properties of natural-based herbal shampoo formulations with Cyclea peltata as a key ingredient.

Synthetic shampoos have toxic chemical agents like chemicals such as parabens and sodium lauryl sulfate which damage the hair. To combat this issue, the use of natural herbal shampoos, as opposed to synthetic shampoos, is becoming increasingly popular. One of the many medicinal plants present in Karnataka and Kerala is Cyclea peltata. This plant has long been known to assist in wound healing, allergy relief, and hair and scalp improvement, as well as serving as a coolant for hair applications. The key subject of this research was the preparation of plant extracts and fermentation of Cyclea peltata leaf extract with Lactobacillus plantarum, resulting in the formulation of an herbal shampoo. The leaf extract contained alkaloids, flavonoids, tannins, phenolic acid, saponins, and proteins, according to previous research by this group. The fermented product showed the presence of lactic acid, which was a boon for shampoo base as it protects the hair from the sunlight and improves the texture and strength of hair fibers. Cyclea peltata was used as the main ingredient in herbal shampoo formulations, along with other herbal ingredients, and the formulated shampoo was characterized for its different organoleptic (Color, odor) and physicochemical properties (pH, solid content, wettability, emulsification, and foam stability) to find the best formulation. Formulation H2 showed better organoleptic and physicochemical properties and thus was chosen as the best formulation among the 11 formulations. Formulation H2 is regarded as the best formulation based on the results of the physicochemical tests (Pleasant odor, pH 3.01, solid content of 5.75%, wettability of 1.68s, and percentage emulsification of 68.75%).

Investigation of Nonlinear Optical Properties of AgNPs Synthesized Using Cyclea peltata Leaf Extract Post OVAT Optimization

Investigation of Nonlinear Optical Properties of AgNPs Synthesized Using Cyclea peltata Leaf Extract Post OVAT Optimization

Evaluation of CYP2D, CYP1A2 and distribution of tetrandrine, fangchinoline in the brain, liver, and kidney of wistar rats after short-term exposure to Cyclea peltata

Background: Cyclea peltata (CP) roots are used in Indian traditional medicine to treat various diseases. However, short-term toxic effects of CP are completely unknown. Objectives: The aim of this study is to evaluate short-term toxic effects of CP, tissue distribution of bioactive alkaloids tetrandrine, fangchinoline in the brain, liver, kidney, and mRNA expression of CYP2D, CYP1A2 after CP administration. Materials and Methods: In vitro toxicity evaluation of CP was carried out using annexin 5/propidium iodide assay with or without CP treatment in L 929 cell line. And in vivo short term toxicity of CP was evaluated in Wistar rats for 28 days. Liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS) based tissue distribution of bioactive molecules was assessed and mRNA expression of CYP1A2, CYP2D, CYP2C6, and CYP2E1 were estimated using qRT- polymerase chain reaction. Results: In vitro toxicity of 70% aqueous ethanol extract of CP 50 μg/mL showed 62.18% cell viability. Oral administration of CP (50, 500 mg/kg) did not cause any clinical, hematological, serum biochemical, and histopathological changes in rats, whereas CP (1000 mg/kg) showed infiltrative changes in the kidney and lungs. Imbalance in oxidative stress and antioxidant defense was reflected as elevated MDA levels in rat liver. LC-Q-TOF-MS studies could detect tissue distribution of bioactive alkaloids tetrandrine, fangchinoline and their decomposed masses in the liver and kidney, whereas tetrandrine and its decomposed molecule (580.3) ([M + H]-43) + could cross blood–brain barrier and were detected in the brain. Evaluation of mRNA expression revealed dose-dependent increase in expression of CYP2D and CYP1A2. Conclusion: Oral administration of CP 500 mg/kg for 28 days is safer in rats due to balanced antioxidant defense. Imbalance in antioxidant defense enzymes and toxic metabolites formed through the escalation of CYP2D, CYP1A2 and metabolism of bioactive alkaloids of CP may be the reason for infiltrative changes in kidney, lungs observed after 28 days CP 1000 mg/kg administration.

Evaluation of Fluoride-Induced Neuro Degenerative Defences in Cyclea Peltata Lam Roots

Many diseases have become easier to diagnose and treat as a result of advancements in medical science and technology, but strokes, which have multiple etiologies and mechanisms, continue to be difficult to treat. Synthetic drugs are notorious for causing a slew of unavoidable side effects. Herbal drugs have a wide range of mechanisms of action and are typically free of side effects, making them excellent alternatives to synthetic drugs for stroke treatment. Cyclea peltata (Lam) Hook f. Thoms roots were studied for their neuroprotective properties against fluoride-induced neurodegeneration in rats. The rat brain homogenate was investigated for the levels of non-enzymatic antioxidants like norepinephrine and serotonin to analyse the health of the brain. Both the hormones norepinephrine and serotonin levels were restored due to the treatment with ethanol extract of the plant. The antioxidant enzyme levels like SOD, CAT, GSH and GPx in the rat brain were estimated, and the results were similar to the non-enzymatic levels. The elevation of antioxidant enzymes indicates that the extract had an antioxidant mechanism that is responsible to help in the prevention of neurodegeneration of rats. In the brain tissues treated with Cyclea peltata extract, there was a significant increase in antioxidant enzymes and a reduction in lipid peroxidation, confirming the antioxidant mechanisms responsible for stroke prevention in extract-treated groups. The root extract of Cyclea peltata was found to show a reasonable inhibitory effect on neurodegeneration when delivered at a dose of 200mg/kg.

Comparative investigation on antimicrobial and phytochemical profiling of Cyclea peltata and Tiliocora acuminate

Comparative investigation on antimicrobial and phytochemical profiling of Cyclea peltata and Tiliocora acuminate

Cyclea peltata Leaf Mediated Green Synthesized Bimetallic Nanoparticles Exhibits Methyl Green Dye Degradation Capability

Nowadays, biological materials are explored widely for green synthesis of nanoparticles because of its ease in scaling up when compared with conventional approaches. In this paper, we report the biosynthesis of bimetallic nanoparticles (FeCuNPs) from their precursors FeSO4 and CuSO4, using Cyclea peltata leaf extract. Biosynthesized nanoparticles were characterized by UV-visible spectroscopy, particle size analyser, FESEM, EDAX, XRD and FTIR. UV-visible spectroscopic investigation confirmed the production of bimetallic core shell nanoparticles at 250 nm, where the colour change in the solution indicated the formation of nanoparticles. The synthesized FeCuNPs were tested for their methyl green dye degradation and degradation kinetics. Results indicated that bimetallic nanoparticles could effectively degrade methyl green dye up to 82% within 105 min, which followed pseudo second order kinetics with R2 of 0.9862. Hence time-dependent reduction in methyl green absorption maxima, obtained from UV-Spectrophotometric analysis and LCMS spectra of degraded dye, confirmed that the green synthesized bimetallic nanoparticles from the leaf extract of Cyclea peltata has the potential of dye degradation.

Phytochemical, antimicrobial, antioxidant and immunomodulatory studies of leaf extracts of Cyclea peltata (Lam.) Hook. f. & Thomson

The objective of the present investigation is to study the phytochemical constituents, antimicrobial, antioxidant activity and immunomodulatory effects of Cyclea peltata leaf extract. The fresh whole plants were collected from Kannur, Kerala, India. The crude extract of leaves of C. peltata prepared using methanol and aqueous solvents and were used to investigate the antibacterial activity against Streptococcus mutans, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae by well diffusion method. Antioxidant by DPPH method and immunomodulatory study using Swiss albino rats. The extracts showed the presence of phytoconstituents like tannin, proteins, resins, carbohydrates, alkaloids and terpenoids. Both the extracts showed varying degree of inhibitory potential against all the tested bacteria. Methanol extract of plant had higher inhibitory action against Pseudomonas aeruginosa and Streptococcus mutans. While Staphylococcus aureus showed low sensitivity against aqueous extract of C. peltata. The body weight of the animals showed constant in the case of lower concentration of the drug (1 mg/kg), whereas at higher concentration, the animals showed reduced body weight, with more reduction in highest concentration. Similarly for the organs like liver, the higher dose showed increased mass of liver when compared to lower doses of the drug. The tested animals showed good immunomodulatory effect against the leaf extract. This study further suggests the use of plant extract in treating disease caused by tested microbial organisms.

IMMUNOMODULATORY, ANTICANCEROUS & ANTIOXIDANTACTIVITIES OF CYCLEA PELTATA (LAM.) HOOK.F. & THOMSON

Introduction: Cyclea peltata (Lam.) Hook.f. & Thomson is an important plant listed in Ayurveda. The plants are used in digestive, diuretic and inflammatory disorders.
 Aim: The present study was to study the immunomodulatory, anticancerous and antioxidative properties of the fraction isolated from Cyclea peltata.
 Methods: The immunomodulatory activity was evaluated in lymphocytes and THP-1 macrophage cell lines. The anticancer activity of the fraction was determined by MTT assay. The antioxidant activity was evaluated by DPPH and total antioxidant assays.
 Results: The fraction showed the presence of flavanoids which induced lymphocyte proliferation and also could induce nitrite production in THP-1 cell lines at 1mg/ml. It also exhibited good anticancerous activity at 100 μg/ml after 48h of incubation. The antioxidant activity was less at this concentration and was found to be equivalent to 79± 0.03 μg/ml of ascorbic acid.
 Conclusion: The fraction containing flavonoid, isolated from Cyclea peltata have shown to have good immunomodulatory, antioxidant and anticancerous property.

Tetrandrine isolated from Cyclea peltata induces cytotoxicity and apoptosis through ROS and caspase pathways in breast and pancreatic cancer cells.

Tetrandrine is a bisbenzylisoquinoline alkaloid known to exhibit anticancer activity against different cancers. In the present study, the cytotoxic effect of tetrandrine isolated from Cyclea peltata on pancreatic (PANC-1) and breast (MDA-MB-231) cancer cells was evaluated in vitro with an attempt to understand the role of tetrandrine on the generation of reactive oxygen species (ROS) and caspase activation. Results demonstrate the dose- and time-dependant cytotoxic effect of tetradrine on both MDA-MB-231 and PANC-1 cells with IC50 values ranging between 51 and 54μM and 22 and 27μM for 24h and 48h of incubation respectively. In addition, treatment of MDA-MB-231 and PANC-1 cells with tetrandrine showed the shrunken cytoplasm and damaged cell membrane in a dose- and time-dependant manner under the microscope. Also, tetrandrine treatment revealed an elevated levels of reactive oxygen species and increased activities of caspase-8, -9 and -3 confirming the apoptosis of cells through both extrinsic death receptor and intrinsic caspase activation. Therefore, the present study suggests the apoptosis of cells with the activation of caspase pathways mainly intrinsic pathway as a downstream event of tetrandrine-induced ROS generation. Hence, reactive oxygen species-mediated caspase activation pathway may be potentially targeted with the use of tetrandrine to treat breast and pancreatic cancers.

Optimization of column chromatography technique for the isolation of tetrandrine from Cyclea peltata and LC-ESI-MS based quantification and validation of the method

Reverse phase column chromatography technique was employed to isolate tetrandrine from methanolic extract of Cyclea peltata roots. Tetrandrine was efficiently isolated using 0.02% aqueous diethylamine and methanol (25:75, v/v) as mobile phase with a purity of 98.63%. Compound characterisation was achieved using high-performance liquid chromatography coupled with electrospray ionization mass spectrometry and other spectroscopic methods. In addition, quantification of tetrandrine and validation of the method was carried out following International Conference on Harmonisation guidelines. The study provides a simple and cost effective method to isolate substantially good quantity and pure tetrandrine from Cyclea peltata.

Ultrastructural and histochemical analysis of the trichomes of Cyclea peltata (Menispermaceae)

Cyclea peltata is a medicinal climber belonging to the family Menispermaceae. The global distribution of this species is recorded from the tropical Asia regions. Trichome phenology studies of C. peltata utilizing Scanning Electron Microscopy (SEM), light microscopy and fluorescent microscopy demonstrated the structural and organizational heterogeneity of trichomes. All the trichomes observed were non-glandular. C. peltata possessed three types of non-glandular trichomes such as long trichomes, short trichomes and ribbon-like trichomes. Of these, ribbon-like trichomes are limited to the surface of the leaves. The majority of the trichomes observed were unicellular, long and hook-shaped. Auto fluorescence analysis revealed a large amount of cell wall materials on the basal cell. Additional morphological features of the trichomes included the occurrence of grooves and deposition of epicuticular wax. The trichome morphology of C. peltata explains the possible influence of environmental cues.

Identification and Quantification of antivenom compounds from aqueous extract of Cyclea peltata root

Snakebite is a severe medical, social and economic failure in many parts of the world, chiefly in the tropical and subtropical nations due to occurrence of majority of the World’s dangerous snakes. In India, a range of medicinal plants are used as antidotes for snakebites, either single or in combination with other. Cyclea peltata is locally called as “Rajpatha” belongs to the family Menispermaceae.Roots are tuberous and are traditionally used in the treatment of snake poisoning and various other diseases. In our present study a variety of secondary metabolites like alkaloids, flavonoids, saponins, phenols, protein, coumarin, reducing sugars, tannins were present in Cyclea peltata root.Various compounds were identified by LCMS analysis which includes copane, deoxyelephantopin, coumaric acid, isovanillic acid, ferulic acid and a-bisabolol.The phenolic compounds mainly Coumaric acid, Ferulic acid and Isovanillic acid were found to possess antivenom activity and were quantified using HPLC analysis.The present study concluded that phenolic compounds present in the root extractof Cyclea peltata root may be responsible for the antivenom activity.