Identification and Quantification of antivenom compounds from aqueous extract of Cyclea peltata root

Abstract

Snakebite is a severe medical, social and economic failure in many parts of the world, chiefly in the tropical and subtropical nations due to occurrence of majority of the World’s dangerous snakes. In India, a range of medicinal plants are used as antidotes for snakebites, either single or in combination with other. Cyclea peltata is locally called as “Rajpatha” belongs to the family Menispermaceae.Roots are tuberous and are traditionally used in the treatment of snake poisoning and various other diseases. In our present study a variety of secondary metabolites like alkaloids, flavonoids, saponins, phenols, protein, coumarin, reducing sugars, tannins were present in Cyclea peltata root.Various compounds were identified by LCMS analysis which includes copane, deoxyelephantopin, coumaric acid, isovanillic acid, ferulic acid and a-bisabolol.The phenolic compounds mainly Coumaric acid, Ferulic acid and Isovanillic acid were found to possess antivenom activity and were quantified using HPLC analysis.The present study concluded that phenolic compounds present in the root extractof Cyclea peltata root may be responsible for the antivenom activity.