Cyclic Strain Research Articles

Hepcidin and erythroferrone response to 3 weeks of exposure to normobaric hypoxia at rest in trained cyclists

Purpose: The effectiveness of altitude training on haematological adaptations is largely dependent on iron metabolism. Hepcidin and erythroferrone (ERFE) are key iron-regulating hormones, yet their response to altitude training is poorly understood. The aim of this study was to analyze changes in hepcidin and ERFE under the influence of 3 weeks of the Live High-Train Low (LH-TL) method.Methods: Twenty male trained cyclists completed a 3-week training program under normoxic conditions (NORM) or with passive exposure to normobaric hypoxia (LH-TL; FiO2 = 16.5%, ∼2000 m; 11–12 h/day). Hepcidin, ERFE, hypoxia inducible factor-2 (HIF-2), ferroportin (Fpn), erythropoietin (EPO), serum iron (Fe) and hematological variables were assessed at baseline (S1), then immediately after (S2) and 3 days after (S3) intervention.Results: In the LH-TL group, hepcidin decreased by 13.0% (p < 0.001) in S2 and remained at a reduced level in S3. ERFE decreased by 28.7% (p < 0.05) in S2 and returned to baseline in S3. HIF-2α decreased gradually, being lower by 25.3% (p < 0.05) in S3. Fpn decreased between S1 and S2 by 18.9% (p < 0.01) and remained lower during S3 (p < 0.01). In the NORM group, in turn, hepcidin levels increased gradually, being higher by 73.9% (p < 0.05) in S3 compared to S1. No statistically significant differences in EPO were observed in both groups.Conclusion: Three weeks of LH-TL suppresses resting hepcidin and ERFE levels in endurance athletes. We found no association between hepcidin and ERFE after LH-TL. Probably, ERFE is not the only factor that suppresses hepcidin expression in response to moderate hypoxia, especially in later stages of hepcidin downregulation. With the cessation of hypoxia, favorable conditions for increasing the availability of iron cease.

Cyclic true stress true strain relation in the large strain region of common steel for welded structures

Cyclic true stress - true strain curves were obtained using general-purpose shipbuilding steel in the large strain region. Test methods are incremental step test and static tensile test after cyclic loading. The stress–strain curves for static and cyclic loading were compared to investigate the cause of the difference between each other. A simple method for estimating the cyclic stress-strain curves was proposed. The diameter and curvature of the smallest cross-section of the specimen were measured using telecentric measurement device, and the true stress - true strain curve was obtained using the Bridgman correction method. Using the measurement method, cyclic stress-strain diagrams were obtained in the region where the true strain exceeded 1% by performing cyclic tensile tests using the incremental step method and static tensile tests after cyclic loading. The influence of the test method and maximum displacement conditions on the cyclic stress-strain curve was small within the scope of this study. There was little difference in the elongation between the static tensile test and the static tensile test after cyclic loading. The difference in yield stress between static and cyclic loading was discussed in terms of macro-yield mechanisms at intergranular and transgranular. A simple method for estimating cyclic stress-strain curves from a static stress-strain curve was proposed. The specimens used in this study are general-purpose shipbuilding steels, and the results should be applicable to similar steels for welding and structures.

Changes in Anthropometric Characteristics and Isokinetic Muscle Strength in Elite Team Sport Players during an Annual Training Cycle

The aim of the present research was to investigate the variation in the anthropometric characteristics and the isokinetic muscle strength of elite female team sport players during a season (29–36 weeks). Three groups of female athletes that consisted of soccer (n = 19; age, 23.2 ± 4.3 years), basketball (n = 26, 21.1 ± 5.4 years) and handball players (n = 26, 21.1 ± 4.2 years) underwent anthropometric and isokinetic measurements at the beginning of the preparation period, in the middle and at the end of the competitive season. Isokinetic peak torque values of the hamstrings (H) and quadriceps (Q), as well as the conventional strength ratios of H:Q, were tested on an isokinetic dynamometer at angular velocities of 60, 180 and 300°·s−1. Body weight, lean body mass and body fat of all groups decreased from the first to the third testing session (p < 0.05). Isokinetic peak torque gradually increased during the three measurements (p < 0.05). The soccer players had lower body weight and body fat compared to the basketball and handball players (p < 0.05). Isokinetic peak torque in knee flexion did not show any difference between the sports at any angular velocity or knee movement (flexion and extension), with an exception of the 180°·s−1. The improvement observed for all athletes can be attributed to the training programs that collectively characterize these team sports.

Strain and Strain Recovery of Human Hair from the Nano- to the Macroscale.

In this study, in operandi SAXS experiments were conducted on samples of human hair with a varying degree of strain (2% within the elastic region and 10% beyond). Four different features in the SAXS patterns were evaluated: The intermediate filament distance perpendicular to and the distance from the meridional arc in the load direction, as well as the distances of the lipid bilayer peak in and perpendicular to the load direction. From the literature, one concludes that polar lipids in the cuticle are the origin of the lipid peak in the SAXS pattern, and this study shows that the observed strain in the lipids is much lower than in the intermediate filaments. We support these findings with SEM micrographs, which show that the scales in the cuticle deform much less than the cortex. The observed deformation of the intermediate filaments is very high, about 70% of the macrostrain, and the ratio of the transverse strain to the longitudinal strain at the nanoscale gives a Poisson ratio of νnano = 0.44, which is typical for soft matter. This work also finds that by varying the time period between two strain cycles, the typical strain recovery time is about 1000 min, i.e., one day. After this period, the structure is nearly identical to the initial structure, which suggests an interpretation that this is the typical time for the self-healing of hair after mechanical treatment.

Post-liquefaction deformation mechanisms of stone column-improved liquefiable sloping ground under cyclic loadings

Liquefaction-induced large deformations in sloping ground caused heavy damage to buildings and infrastructures during earthquakes, and its evaluation and mitigation challenge. In this study, a series of soil element tests using hollow cylinder apparatus (HCA) were conducted to investigate the relationship between residual volumetric strain and residual shear strain of medium dense to dense saturated sand with moderate initial static shear stress. The soil element tests indicate that the developments of residual volumetric strain and residual shear strain are dominated by the Post-liquefaction Deformation Potential (PLDP) of soil, which is well correlated to the maximum cyclic shear strain developed during cyclic loading. Then, the applicability of PLDP to characterize the post-liquefaction deformation response in gently sloping ground was investigated by centrifuge model tests without and with stone column improvement. The model tests of medium dense and dense sand slopes proved the applicability of PLDP preliminarily. The mitigation mechanisms against settlement and lateral spreading in gentle slopes by densification and drainage effects induced by stone columns were also observed and discussed. The present study provides the conceptual term of PLDP for evaluating post-liquefaction deformations of natural and stone column-improved gently sloping grounds, which helps to develop mitigation techniques for liquefiable sloping ground subjected to earthquake loadings.

Optimization of a LaNiO3 Bottom Electrode for Flexible Pb(Zr,Ti)O3 Film-Based Ferroelectric Random Access Memory Applications

The direct growth of ferroelectric films onto flexible substrates has garnered significant interest in the advancement of portable and wearable electronic devices. However, the search for an optimized bottom electrode that can provide a large and stable remnant polarization is still ongoing. In this study, we report the optimization of an oxide-based LaNiO3 (LNO) electrode for high-quality Pb(Zr0.52Ti0.48)O3 (PZT) thick films. The surface morphology and electrical conductivity of sol-gel-grown LNO films on a fluorophlogopite mica (F-mica) substrate were optimized at a crystallization temperature of 800 °C and a film thickness of 120 nm. Our approach represents the promising potential pairing between PZT and LNO electrodes. While LNO-coated F-mica maintains stable electrical conductivity during 1.0%-strain and 104-bending cycles, the upper PZT films exhibit a nearly square-like polarization–electric field behavior under those stress conditions. After 104 cycles at 0.5% strain, the remnant polarization shows decreases as small as ~14%. Under flat (bent) conditions, the value decreases to just 81% (49%) after 1010 fatigue cycles and to 96% (85%) after 105 s of a retention test, respectively.

Impact of laser powder bed fusion processing on the cyclic and fatigue properties of Ni20Cr alloy

Additive manufacturing is a new-age technology specialising in intricate fabrication in the manufacturing industry. However, extremely high cooling rates and far-from-equilibrium kinetics produce heterogeneous microstructure and induce high initial dislocation density, porosity, and residual stress. These unique microstructural features are known to severely impact cyclic and fatigue properties of alloys. In such a context, we investigated the cyclic and fatigue properties of a Ni-20 wt%. Cr alloy manufactured via laser powder bed fusion process (LPBF) and compared with its cast counterpart. The fatigue testing was carried out with three different strain amplitudes depicting Low, Medium and High Cycle Fatigue (LCF, MCF, HCF). LPBF samples exhibited higher fatigue resistance than that of cast samples, however experienced early failure in all the conditions. Despite strong planar glide, LPBF samples exhibited softening behaviour; the degree of softening is similar for LCF and MCF and less pronounced for HCF. The cast samples did not undergo any softening whatsoever indicating huge differences in cyclic strain mechanisms. Masing analysis was proposed to graphically analyse this softening behaviour. Further, the flow stress was categorised into backstress and effective stress for LPBF and cast samples. Majority of softening in LPBF samples for LCF and MCF occurred via backstress, whereas effective stress is associated to HCF. To investigate the origin of this softening for LPBF samples, microstructure characterisations were performed during the softening and at fracture. Post-fatigue microstructure indicates a clear modification of the dislocation structures from those inherited from LPBF to those linked to fatigue. This gradual change in microstructure is expected to induce the cyclic softening.

Printable, adhesive, and self-healing dry epidermal electrodes based on PEDOT:PSS and polyurethane diol

Printable, self-healing, stretchable, and conductive materials have tremendous potential for the fabrication of advanced electronic devices. Poly(3,4-ethylenedioxithiopene) doped with polystyrene sulfonate (PEDOT:PSS) has been the focus of extensive research due to its tunable electrical and mechanical properties. Owing to its solution-processability and self-healing ability, PEDOT:PSS is an excellent candidate for developing printable inks. In this study, we developed printable, stretchable, dry, lightly adhesive, and self-healing materials for biomedical applications. Polyurethane diol (PUD), polyethylene glycol, and sorbitol were investigated as additives for PEDOT:PSS. In this study, we identified an optimal printable mixture obtained by adding PUD to PEDOT:PSS, which improved both the mechanical and electrical properties. PUD/PEDOT:PSS free-standing films with optimized composition showed a conductivity of approximately 30 S cm−1, stretchability of 30%, and Young’s modulus of 15 MPa. A low resistance change (<20%) was achieved when the strain was increased to 30%. Excellent electrical stability under cyclic mechanical strain, biocompatibility, and 100% electrical self-healing were also observed. The potential biomedical applications of this mixture were demonstrated by fabricating a printed epidermal electrode on a stretchable silicone substrate. The PUD/PEDOT:PSS electrodes displayed a skin-electrode impedance similar to commercially available ones, and successfully captured physiological signals. This study contributes to the development of improved customization and enhanced mechanical durability of soft electronic materials.

Research on a Method for Online Damage Evaluation of Turbine Blades in a Gas Turbine Based on Operating Conditions

Performing online damage evaluation of blades subjected to complex cyclic loads based on the operating state of a gas turbine enables real-time reflection of a blade’s damage condition. This, in turn, facilitates the achievement of predictive maintenance objectives, enhancing the economic and operational stability of gas turbine operations. This study establishes a hybrid model for online damage evaluation of gas turbine blades based on their operational state. The model comprises a gas turbine performance model based on thermodynamic simulation, a component load calculation model based on a surrogate model, an updated cycle counting method based on four-point rainflow, and an improved damage mechanism evaluation model. In the new model, the use of a surrogate model for the estimation of blade loading information based on gas turbine operating parameters replaces the conventional physical modeling methods. This substitution enhances the accuracy of blade loading calculations while ensuring real-time performance. Additionally, the new model introduces an updated cycle counting method based on four-point rainflow and an improved damage mechanism evaluation model. In the temperature counting part, a characteristic stress that represents the stress information during the cyclic process is proposed. This inclusion allows for the consideration of the impact of stress fluctuations on creep damage, thereby enhancing the accuracy of the fatigue damage assessment. In the stress counting part, the model incorporates time information associated with each cycle. This concept is subsequently applied in determining the identified cyclic strain information, thereby improving the accuracy of the fatigue damage evaluation. Finally, this study applies the new model to an online damage evaluation of a turbine stationary blade using actual operating data from a micro gas turbine. The results obtained from the new model are compared with the EOH recommended by the OEM, validating the accuracy and applicability of the new model.

The Effects of EEG Biofeedback Training on Visual Reaction Time in Judo Athletes.

The aim of the study was to expand the current knowledge on the effects of EEG biofeedback training on the reaction time of judo athletes, as well as to develop an optimal EEG training protocol in terms of the number of sessions and their duration that would significantly improve the reaction time of athletes. The study included 24 male athletes from the national team of the Polish Judo Association. The selected group was randomly divided into two subgroups: experimental and control. The study was conducted in four cycles varying in terms of frequency and duration of neurofeedback (NFB) sessions, both in the control and experimental groups. In the experimental group, each training cycle consisted of 15 sessions, followed by a four-week break. The effects of NFB training on the visual reaction time of judo athletes were evaluated using computerized simple and complex reaction time tests along with selected trials of the Vienna Test System (VTS). Following NFB training according to the theta/beta1 protocol, while maintaining appropriate duration and frequency of individual training sessions, statistically significant improvements in reaction times to visual stimuli of athletes, both in simple and complex tasks, were observed in the experimental group. No such changes were found in the control group. The greatest improvement in reaction times was observed in complex tasks, indicating the high effectiveness of EEG biofeedback training in enhancing this ability.

Effect of Shot Peening on the Low-Cycle Fatigue Behavior of an AA2519-T62 Friction-Stir-Welded Butt Joint.

In this investigation, an AA2519-T62 FSW butt joint was subjected to shot peening with an air pressure of p = 0.6 MPa, a processing time of t = 10 min (per side), and a steel ball diameter of dk = 1.5 mm. In order to evaluate the impact of shot peening on the low-cycle behavior, the samples were tested with coefficient R = 0.1 at total strain amplitudes of 0.35%, 0.4%, and 0.5%. The shot-peened welds are characterized by a higher value of stress amplitude, a lower value of plastic strain amplitude, and their fatigue life increased slightly. The cyclic strength coefficient and the cyclic strain hardening exponent were reduced by 45% and 55%, respectively, as the result of the surface layer hardening. The shot peening process had no noticeable effect on the character of crack initiation and propagation. Almost in all cases, the cracking started in the area under the weld face, located close to the boundary between the thermo-mechanically affected zone and the stir zone at the advancing side. Only at the heaviest loadings (εac = 0.5%) were cracks initiated in the heat-affected zone at the retreating side. Despite the introduction of small cracks in the stir zone, their presence did not affect the decohesion character of the welded joint. Overall, it was observed that there is a minimal, positive impact of shot peening on the properties of the investigated joints.

Abstract 13736: Whole Body versus Small Muscle Mass Exercise Training in Patients With Heart Failure With Preserved Ejection Fraction: Impact on Peripheral Responses to Exercise

Background: Patients with heart failure with preserved ejection fraction (HFpEF) have reduced peak aerobic power (VO 2 ) during whole body exercise due in part to smaller increases in blood flow (BF) and arterial-to-venous oxygen difference (Δa-vO 2 ). We recently demonstrated that 8wks of single leg knee extension (SLKE) exercise training was effective in improving whole body VO 2 by increasing Δa-vO 2 in HFpEF; whether SLKE training induces greater peripheral adaptations compared to traditionally prescribed whole-body (i.e., cycle) training is unknown. Hypothesis: SLKE training will result in larger improvements in peak leg VO 2 , leg BF, and Δa-vO 2 during a SLKE peak exercise test vs cycle training. Methods: We recruited 16 patients with HFpEF (71 ± 6yr, 5 males) and randomized them to cycle or SLKE training 3-4 days/wk for 16wk. Before and after the intervention we quantified leg BF (ultrasound), femoral venous O 2 partial pressure (PvO 2 ) and saturation (IV catheter) during incremental SLKE peak exercise to determine peak leg VO 2, Δa-vO 2, and skeletal muscle diffusive conductance (DMO 2 ; leg VO 2 /[2x leg PvO 2 ]). Results: SLKE (n=9) and cycle (n=7) groups were matched for age and sex. Both groups increased peak workload during the SLKE exercise test (34±8%; mean ± SE; p=0.005). There was a main effect of training such that peak DMO 2 improved in both groups (cycle: 45±19%; SLKE: 12±3%; see Figure 1 ). Peak Δa-vO 2 also improved (main effect of training, p=0.002); however, this was greater in response to cycle compared to SLKE training (12±3% vs 3±2%; interaction effect, p=0.035). Conclusion: These preliminary data indicate that both types of training improve leg DMO 2 ; however, greater improvements in O 2 extraction occurred with cycle training. The differences in the peripheral adaptations may be attributed to a larger exercise “dose” in the cycle trained group. This data highlights that both modalities are effective components of exercise prescription for patients with HFpEF.

A Novel Approach for Detecting Outliers by Using Isolation Forest with Reducing Under Fitting Issue

The effectiveness of machine learning for a particular activity depends on a variety of parameters. The incident database's description and validity come first and primary. Information retrieval even during the training cycle is more challenging if there is a lot of repetitious, unimportant information or incomplete information available. It is good knowledge that running time for ML tasks is significantly impacted by conditions as follows and sorting stages. To increase the accuracy of any model data cleansing is essential. Without sufficient data scrubbing, no predictive model accuracy can begin. EDA, or exploratory data analysis, is the name of this procedure. In this study, we discussed outlier identification, one of many EDA processes for complete perfect data. In this research, we attempted to use the isolation forest approach to calculate the outlier factor. Then a model known as an outlier finding model is created. The problem of outlier detection leads to a collection of connected supervised learning for binary classification. We carry out in-depth tests on various datasets and demonstrate that in our latest outlier finding technique compare with the old way. Our approach yields superior outcomes in terms of accuracy, precision, recall &amp; F-1 score. Additionally, we successfully lowered the machine learning algorithms' under fitting issue.

Mouth Rinsing and Ingestion of Unpleasant Salty or Bitter Solutions Does Not Improve Cycling Sprint Performance in Trained Cyclists.

The purpose of this study was to investigate the influence of mouth rinsing and ingesting unpleasant salty or bitter solutions on cycling sprint performance and knee extensor force characteristics. Eleven male and one female trained cyclists (age: 34 ± 9years, maximal oxygen uptake 56.9 ± 3.9ml·kg-1·min-1) completed a ramp test and familiarization followed by four experimental trials. In each trial, participants completed an all-out 30-s cycling sprint with knee extensor maximal voluntary contractions before and immediately after the sprint. In a randomized, counterbalanced, cross-over order, the four main trials were: a no solution control condition, water, salty (5.8%), or bitter (2mM quinine) solutions that were mouth rinsed (10s) and ingested immediately before the cycling sprint. There were no significant differences between conditions in mean power (mean ± SD, no solution: 822 ± 115W, water: 818 ± 108W, salt: 832 ± 111W, bitter: 818 ± 105W); peak power (no solution: 1,184 ± 205W, water: 1,177 ± 207W, salt: 1,195 ± 210W, bitter: 1,184 ± 209W); or fatigue index (no solution: 51.5% ± 5.7%, water: 50.8% ± 7.0%, salt: 51.1% ± 5.9%, bitter: 51.2% ± 7.1%) during the sprint. Maximal force and impulse declined postexercise; however, there were no significant differences between conditions in knee extensor force characteristics. The present data do not support the use of unpleasant salty or bitter solutions as an ergogenic aid to improve sprint exercise performance.

Heat transfer analysis and thermo-mechanical property of M-shape metal-rubber at high-temperature

Particular attention is given to the M-shape metal-rubber (MMR), which is a porous material with cushioning characteristics and excellent heat resistance coated in pipelines. The heat transfer characteristics and thermo-mechanical property of MMR has not been clear due to its complicated micro-porous structures and the coupling effect between structure and performance. In this work, the influence of different parameters (emissivity, density and gap distance) on the equivalent thermal conductivity of MMR were considered at the mesoscopic model level. In addition, thermal stress at different temperatures was used as predefined fields for thermal-mechanical coupling to analyze the transformations of performance. The results indicate that surface emissivity and cavity emissivity during heat transfer exhibit an inverse relationship with MMR thermal conductivity. Furthermore, it is observed that natural convection has the most significant impact on thermal conductivity. And, the thermal conductivity is negative correlation with density and gap distances. Under cyclic loading, stress concentration and plastic strain occur in MMR, and residual thermal stress is generated. The secant stiffness exhibits a positive correlation with temperature, while the loss factor demonstrates a negative correlation, primarily due to thermal expansion.

Eccentric Cycling Is an Alternative to Nordic Hamstring Exercise to Increase the Neuromuscular Function of Knee Flexors in Untrained Men.

Valdes, O, Inzulza, S, Collao, N, Garcia-Vicencio, S, Tufano, JJ, Earp, J, Venegas, M, and Peñailillo, L. Eccentric cycling is an alternative to Nordic hamstring exercise to increase the neuromuscular function of knee flexors in untrained men. J Strength Cond Res 37(11): 2158-2166, 2023-Nordic hamstring exercise (NHE) has been proposed to reduce knee flexor (KF) injuries. However, submaximal alternatives to NHE are necessary for the clinical or weaker population. The aim of this study was to compare the effects of Nordic hamstring training (NHT) and eccentric cycling (ECC) training on the neuromuscular function of the KF. Twenty healthy men (27.7 ± 3.5 years) were randomly assigned into 2 groups that performed 10 training sessions (2-3 sessions·week-1) of either NHT (n = 10) or ECC (n = 10). Maximal voluntary isometric contraction of the KF and knee extensor (KE) muscles (MVICKF and MVICKE) was measured, and the hamstring/quadriceps strength (H/Q) ratio was calculated. Furthermore, changes in NHE maximum reaction force (NHE-MRFKF), NHE break-point angle (NHE-BPA), and muscle activity of the semitendinosus (STEMG) and biceps femoris (BFEMG) during the NHE after the interventions were compared. Although no group × time effects were observed (p = 0.09-0.70), but time effects were found for all variables. Pairwise comparisons revealed that MVICKF (+16.9%; p = 0.02), H/Q ratio (+11.8%; p = 0.01), NHE-MRFKF (+19.8%; p = 0.005), and NHE-BPA (+30.8%; p = 0.001) increased after ECC, whereas NHE-MRFKF (+9.7%; p = 0.003), NHE-BPA (+35.5%; p = 0.0002), and STEMG (+33.7%; p = 0.02) increased after NHT. A group × time effect was observed (p = 0.003) in BFEMG, revealing an increase only after ECC (+41.1%; p < 0.0001). Similar neuromuscular adaptations were found after both training modalities. Therefore, ECC provides similar adaptations as NHT and may serve as an alternative form of KF training for those unable to perform NHE.

Effect of Adherend Thickness on Near-Field Ultrasonic Welding of Single-Lap CF/LMPAEK Thermoplastic Composite Joints

Ultrasonic welding is a fast and promising joining technique for thermoplastic composite parts. Understanding how changing the part thickness affects the process is crucial to its future upscaling and industrialization. This article presents an initial insight into the effect of the adherend’s thickness on the near-field ultrasonic welding of CF/LMPAEK thermoplastic composites. Different thicknesses of the top and bottom adherend were welded and analyzed using the output data of the welding equipment, temperature measurements, and other visual characterization techniques. Increasing the thickness of both the top and the bottom adherends showed to increase the power consumed during welding. An overshoot in the power needed at the onset of the welding process for increased thickness of the top adherend precluded welding beyond a threshold thickness of 4.72 mm. In the case of the thicker top adherends, there was also melting of the energy director and early fiber squeeze-out within the top adherend as a result of increased bulk heating. Increased bulk heating was hypothesized to be caused by increased hammering, as indicated by the amplitude readings for thicker adherends. Welding with a higher force, which is known to reduce hammering, corroborated this hypothesis as fiber squeeze-out within the top adherend was not observed. It is believed that hammering contributes to heating by causing an oscillatory impact excitation that is close to the natural frequencies of the system, which would result in amplification of the cyclic strain and subsequent increase in the viscoelastic heating in the adherend.

The cellular responses of corneal fibroblasts to cyclic stretching loads

Mechanical signaling plays a crucial role in maintaining extracellular matrix (ECM) homeostasis in various structures. In this study, we investigated the responses of corneal fibroblasts to cyclic stretching loads using an in vitro cell culture system. Bovine corneal fibroblasts were cultured and subjected to equibiaxial cyclic strain of 15% for 72 h at a frequency of 0.25 Hz, with bovine skin fibroblasts used as a comparison. We explored various cellular behaviors, including morphological changes, cell proliferation, and metabolism in response to mechanical stretching loads. The expression of genes, protein secretion, and enzymatic activity for several major metalloproteinases was also determined through Q-PCR, Western blot, and gel zymography. Additionally, we investigated the involvement of mitogen-activated protein kinases (MAPKs) signaling pathways in the corneal fibroblasts when subjected to mechanical stimuli. Our findings revealed that, compared to skin fibroblasts, corneal fibroblasts were reluctant to morphological changes in response to a prolonged (72 h) and high-amplitude (15% of strain) cyclic stretching load. However, cyclic stretching loads stimulated the upregulation of MMP-2 expression in corneal fibroblasts via the MAPK signaling pathways involving extracellular signal-regulated kinase and p38. Together with a lack of upregulation in type I collagen expression, our results indicate the induction of the ECM degradation process in corneal fibroblasts in response to cyclic stretching. These findings emphasize the mechanoresponsive nature of corneal fibroblasts and shed light on the potential impact of intense mechanical stress on the cornea in both normal and pathological conditions such as keratoconus, providing valuable insights for understanding corneal mechanobiology.

Rapid recovery in a patient with severe COVID-19 after a low-load, high-frequency rehabilitation program using an ergometer in the supine position.

Background Rehabilitation therapy for patients with severe coronavirus disease 2019 (COVID-19) is crucial; however, studies regarding rehabilitation strategies for intensive care unit (ICU) patients with COVID-19 are limited. We report a case of severe COVID-19 in an ICU patient whose physical function and basic movement ability rapidly improved after the initiation of active aerobic exercise in the supine position.Case A 70-year-old man with hypertension and obesity was admitted to the ICU and managed with a ventilator because of severe COVID-19. Physical therapy started on postadmission day 34. Problems encountered during physical therapy included low saturation of percutaneous oxygen (SpO2; <90%), dyspnea with a light exercise load, advancing muscle weakness, and endurance decline. The rehabilitation program included getting out of bed and resistance training of the upper and lower limbs twice daily while maintaining SpO2 at ≥90%. After ventilator weaning, we initiated aerobic training using a supine ergometer with varying load volume. On discharge from the ICU on postadmission day 45, the patient' s physical function (handgrip strength, Medical Research Council score, and Borg scale) and basic movement ability (Functional Status Score for ICU) rapidly improved.Conclusion Rehabilitation therapy involving aerobic cycling training based on a quantitative load setting may be effective in treating COVID-19.

A systematic investigation of the effects of TGF-β3 and mechanical stimulation on tenogenic differentiation of mesenchymal stromal cells in a poly(ethylene glycol)/gelatin-based hydrogel

BackgroundHigh post-surgical failure rates following tendon injury generate high medical costs and poor patient recovery. Cell-based tendon tissue engineering has the potential to produce fully functional replacement tissue and provide new strategies to restore tendon function and healing. In this endeavour, the application of mesenchymal stromal cells (MSCs) encapsulated in biomaterial scaffolds has shown great promise. However, a consensus on optimal promotion of tenogenic differentiation of MSCs has yet to be reached, although growth factors and mechanical cues are generally acknowledged as important factors. MethodsIn this study, we prepared a hydrogel cell culture system consisting of methacrylated poly(d,l-lactic acid-ethylene glycol-d,l-lactic acid) (P(LA-EG-LA)) and gelatin methacrylate (GelMA) to encapsulate human bone marrow-derived MSCs (hBMSCs). We further systematically investigated the influence of static and intermittent cyclic uniaxial strain mechanical stimulation, in combination with transforming growth factor-β3 (TGF-β3) supplementation, on tenogenic differentiation of hBMSCs. ResultsIncreased TGF-β3 concentration upregulated the tenogenic genes Scleraxis (SCX) and collagen type I (COL1A1) but showed no effects on tenascin-c (TNC) and collagen type III (COL3A1) expression. Mechanical stimulation had no observable effect on gene expression, but intermittent cyclic uniaxial strain stimulation improved matrix deposition. Together, these data provide new insights into how TGF-β3 and mechanical stimulation regulate MSC tenogenesis, with TGF-β3 promoting the expression of key tenogenic genes whilst mechanical stimulation aided matrix deposition in the engineered tissue. Furthermore, intermittent cyclic uniaxial strain at 3% elongation and 0.33 ​Hz for 1 ​h/day showed improved matrix effects compared to static strain. ConclusionTogether, the most promising result for tenogenic differentiation of hBMSCs was identified as treatment with 5 ​ng/ml TGF-β3 under intermittent cyclic uniaxial strain (3% strain; 0.33 ​Hz; 1 ​h/day). This knowledge is of importance for the development of an improved protocol for tenogenic differentiation of MSCs and thereby for tendon tissue engineering. The translational potential of this articleTissue-engineered strategies for tendon repair require a consensus on the differentiation of mesenchymal stromal cells to tenocytes, which is currently lacking. This article provides a systematic investigation of two main tenogenic differentiation conditions to further development of a tenogenic differentiation protocol.