The burgeoning evolution of smart cities, characterized by the integration of the Internet of Things (IoT), Artificial Intelligence (AI), and Machine Learning (ML), heralds a transformative era in urban management and citizen engagement. These technological advancements promise enhanced efficiency in city operations, improved public services, and a sustainable urban environment. However, the complexity and interconnectedness inherent in these systems introduce significant cybersecurity challenges, necessitating innovative approaches to safeguard the digital infrastructure of smart cities. This paper aims to explore the cybersecurity landscape of smart cities from the perspective of integrating IoT, AI, and ML for the creation of digital twins, offering a comprehensive analysis of the opportunities and threats within this domain. Smart cities leverage IoT to connect various components of the urban infrastructure, including transportation systems, utilities, and public services, creating an integrated network of devices that communicate and share data. The incorporation of AI and ML into this framework facilitates intelligent decision-making, enabling the automation of services and the optimization of resources. This synergy enhances the quality of life for residents, promotes economic development, and supports sustainable environmental practices. However, the dependence on digital technologies also exposes smart cities to a range of cybersecurity risks, from data breaches and privacy violations to the disruption of critical infrastructure. The integration of IoT, AI, and ML in smart cities, while offering unprecedented opportunities for urban innovation, also amplifies the complexity of the cybersecurity landscape. IoT devices, often designed with minimal security features, become potential entry points for cyber attacks. The vast amount of data generated and processed by these devices, if compromised, could lead to significant privacy and security breaches. AI and ML models, for their part, are susceptible to manipulation and bias, which can undermine the integrity of decision-making processes. The interconnectivity of systems means that a breach in one sector could have cascading effects throughout the city's infrastructure. Against this backdrop, the paper investigates the role of digital twins in mitigating cybersecurity risks in smart cities. Digital twins, digital replicas of physical entities or systems, offer a powerful tool for simulating and analyzing smart city operations, including cybersecurity scenarios. By mirroring the city's infrastructure in a virtual environment, digital twins allow for the identification of vulnerabilities, the simulation of cyber attacks, and the evaluation of potential impacts. This proactive approach to cybersecurity enables city administrators to anticipate threats and implement protective measures before real-world systems are compromised. The research questions guiding this inquiry include: How can the integration of IoT, AI, and ML enhance the resilience of smart cities against cyber threats? What are the specific cybersecurity challenges presented by these technologies, and how can they be addressed? And, most crucially, what role can digital twins play in fortifying the cybersecurity defenses of smart cities? To address these questions, the paper begins with a review of the current state of smart city technology, focusing on the integration of IoT, AI, and ML. It then delves into the cybersecurity challenges unique to this technological landscape, drawing on recent examples of cyber incidents in smart cities. The analysis highlights the vulnerabilities introduced by the widespread use of IoT devices and the complexities of securing AI and ML systems. Following this, the discussion turns to the potential of digital twins as a cybersecurity tool, examining how they can be employed to detect vulnerabilities, simulate attacks, and plan responses. The paper argues that while the integration of IoT, AI, and ML in smart cities presents significant cybersecurity challenges, it also offers opportunities for innovative solutions. Digital twins emerge as a promising approach to enhancing the cybersecurity posture of smart cities, enabling a dynamic and proactive defense mechanism. By facilitating the simulation of cyber threats in a controlled environment, digital twins allow city administrators to identify weaknesses, test the efficacy of protective measures, and develop more resilient urban infrastructures. In conclusion, the integration of IoT, AI, and ML in smart cities represents a double-edged sword, offering both remarkable opportunities for urban innovation and formidable cybersecurity challenges. This paper underscores the critical importance of adopting a cybersecurity perspective in the development and management of smart cities, highlighting the potential of digital twins as a strategic tool in mitigating these risks. As smart cities continue to evolve, embracing these technologies in a secure and responsible manner will be paramount in realizing their full potential while safeguarding the digital and physical well-being of urban populations.