Shoot Cuttings Research Articles

Potential propagation seedling of Ramie (Boehmeria nivea) from various types of stem cuttings

Ramie is a fiber-producing plant that can replace cotton as a raw material for the textile industry. In general, ramie plants are reproduced using rhizomes, but it takes a long time, around two years, to be used as a source of planting material. Therefore, other sources of explants besides rhizomes are required, such as stem cuttings. Explants from stem cuttings are quickly available and only need 2-3 months to be used as planting material. This study aims to determine the potential of effective stem cuttings that can be used as planting material for the propagation of ramie seedlings. The research was conducted from July to December 2021 at the experimental garden in the Indonesian Instruments Standardization Testing Center for Sweetener and Fiber Crops, Malang, East Java. The research used a Completely Randomized Design (CRD) consisting of four treatments of ramie seedling material of the Ramindo 1 variety, namely rhizome, shoot cuttings, middle stem cuttings, and basal stem cuttings. Each treatment was repeated 5 times. The result showed that all parameters had a significant effect. Ramie seedling source from basal stem cuttings showed the best growth percentage (92%), stem diameter (4.8 mm), and plant wet weight (44.6 g). Shoot cuttings showed the best number of roots (31.6), plant height (60.8 cm), and number of leaves (41.4). Cuttings from shoot and basal stems can be used as planting material for producing ramie seeds other than rhizomes.

CONSERVATION STATUS AND PROPAGATION OF Camellia dalatensis AND Camellia capitata BY CUTTINGS

Article Highlights- Camellia dalatensis and Camellia capitata are critically endangered species.- Habitat loss and deforestation threaten the survival of these Camellia species.- Vegetative propagation aids conservation of critically endangered Camellia species.- Rooting success depends on cutting types, growth regulators, and substrate mix.- Research aids conservation efforts for Vietnam’s rare and vulnerable flora. AbstractCamellia dalatensis (V. D. Luong, Ninh & Hakoda) and Camellia capitata (Orel, Curry & Luu) are classified as critically endangered (CR) by the IUCN. Conservation solutions for these two species have not yet been fully implemented, while wild populations are declining. This research on vegetative propagation examined the efficacy of indole-3-butyric acid (IBA in solution or in powdered activated charcoal), cutting types (terminal shoot or stem cuttings), and substrates for the growth of cuttings (sand-coir dust mixes) on rooting efficiency. The highest rooting of C. dalatensis was observed in terminal shoot cuttings at 1,000 ppm IBA, and 25% sand and 75% coir dust substrate. Maximum values obtained were survival 95.6%, rooting percentage 88.9%, number of roots per cutting 6.9, root length 6.2 cm, and rooting index 42.9. For C. capitata, superior rooting was obtained with semi-hardwood cuttings, at 1,500 ppm IBA, and equal parts of sand and coir dust as substrate. Camellia capitata was more difficult to root, with maximum values of 65.6% survival, 52.2% rooting, 2.8 roots per cutting, 2.9 cm root length, and rooting index of 8.1. The results of this study can be used to support further propagation and conservation of these two endangered species.

Low-Molecular-Weight Organic Acid as an Alternative to Promote the Rooting of Persimmon Rootstock Shoot Cuttings.

Organic acids are naturally present in plants and exert a positive influence on plant development, which justifies surveying their potential effect on adventitious root (AR) formation. In this study, 0.0298 mol/L (4000 mg/L) of malic acid and 0.0267 mol/L (4000 mg/L) of tartaric acid were used to explore the effects of low-molecular-weight organic acid on the rooting of persimmon rootstock Diospyros lotus L. during cutting propagation. After organic acid treatment, the rooting percentage and the survival rate significantly increased, accompanied by a greater development of lateral roots. Anatomical analysis revealed that Diospyros lotus L. exhibits characteristics that induce root primordia, and organic acid treatment can enhance the differentiation of root primordia. Furthermore, treatment with organic acid led to a substantial decrease in soluble sugar and starch contents, along with a slight increase in soluble protein content during early cutting stages. Additionally, the indole-3-acetic acid (IAA) content peaked in the early stages of AR formation and was significantly higher than that of the control, while abscisic acid (ABA) levels exhibited the opposite trend. Comparatively, gibberellic acid (GA3) remained at extremely low levels throughout the rooting process in the organic acid groups compared to the control. In conclusion, the current study uncovers the anatomical structure over time during AR formation, revealing the dynamic changes in the related main nutrients and hormones and providing new ideas and a new practical approach for improving root regeneration in persimmon rootstock cuttings.

PENGARUH PEMBERIAN PLANT GROWTH PROMOTING RHIZOBACTERIA (PGPR) PADA PERTUMBUHAN BIBIT STEK STEVIA (Stevia rebaudiana Bertoni)

Sweetleaf stevia (Stevia rebaudiana Bertoni) is one of the potential sugar-producing plants besides sugar cane which is widely used as a natural non-calorie sweetener. Propagation of stevia by shoot cuttings produces more uniform seedlings. Plant Growth Promoting Rhizobacteria (PGPR) contains a group of beneficial bacteria that live in the plant root ecosystem that can stimulate plant growth. This study aims to determine the effect of PGPR on the growth of stevia cuttings and the best concentration of PGPR on the growth of stevia cuttings. This study was conducted in February-May 2024 in Kolongan Atas II Village, Sonder District, Minahasa Regency, North Sulawesi Province. The study used a Completely Randomized Design (RAL), with 5 treatment levels, namely P0 = Control (Without PGPR), P1 = PGPR 10 ml/liter of water, P2 = PGPR 20 ml/liter of water, P3 = PGPR 30 ml/liter of water and P4 = PGPR 40 ml/liter of water. Each treatment was repeated 4 times.The results showed that the aplication of PGPR affected the growth of stevia cuttings. The best PGPR concentration for the growth of stevia cuttings was at a PGPR treatment concentration of 20 ml/liter of water. Keywords: plant growth promoting rhizobacteria, stevia

Growth of Indigofera zolingeriana with differences in source of cuttings and planting media

This study investigates the effect of different cutting types and planting media on the growth of Indigofera zollingeriana, a valuable forage crop. The research used a factorial randomized block design (2 x 2) with 40 experimental units. The treatments included two cutting sources—stem cuttings (A1) and shoot cuttings (A2)—and two planting media—natural soil (B1) and natural soil supplemented with liquid organic fertilizer from rabbit urine (B2). Growth parameters such as the number of leaves, plant height, and stem diameter were measured. The results indicate that cutting type significantly affects leaf production, with shoot cuttings (A2) producing a higher number of leaves than stem cuttings (A1) (P<0.05). However, the planting media and its interaction with the cutting type had no significant impact on plant height and stem diameter (P>0.05). These findings suggest that using shoot cuttings could enhance the propagation and growth of Indigofera zollingeriana for sustainable forage production. Further studies are recommended to explore the long-term effects of organic fertilizer on biomass yield.

Impact of Iba and Naa Concentrations on Growth Characteristics of MR2 Mulberry (Morus sinensis) Using Mini-Clonal Technology at Nursery Level

Using mini-clonal technology, an experiment was carried out to examine the effects of rooting hormone on the growth and rooting characteristics of the mulberry (Morus sinensis L.) variety 'MR2'. Cuttings from the apical shoots of the mulberry variety MR2, which is the popular variety among farmers and have good resistance towards mildew attack was taken for the study. The experiment was conducted at the Department of Sericulture, Forest College and Research Institute, Mettupalayam. Four replications of the Completely Randomized Design (CRD) experiment were used to arrange the data. Two hormones, such as Indole-3-butyric acid (IBA) and Napthalene acetic acid (NAA), were used in the experiment. The hormones were replicated four times in each of the following concentration ranges: 1000, 2000, 3000, 4000, and 5000 ppm. Different hormone concentrations were applied to apical shoot cuttings before they were planted in the appropriate rooting medium in a greenhouse. At the 90th day, measurements were taken of the following parameters like shoot length (cm), number of roots per plant (no’s), root length (cm) and number of leaves per plant (no’s). IBA and NAA at 5000 ppm out performed all other treatments in terms of mulberry mini cutting growth and root characteristics.

Influence of rooting hormone and rooting substrate on growth of apical shoot cuttings of mulberry (Morus indica l.) Using mini clonal technology at nursery level

An experiment was conducted at Department of Sericulture, Forest College and Research Institute, Mettupalayam to analyse the effect of rooting hormone and their concentrations, rooting medium on apical shoot cuttings of mulberry. Auxin with various concentrations namely IBA and NAA (1000, 2000, 3000, 4000 and 5000 ppm) and rooting mediums such as Soil, Soil: Sand: FYM (1:1:1), Soil: Coir pith (1:1), Soil: Sand: Vermicompost (1:1:1) and Soil: Coir pith: FYM (1:1:1) were used for this study. Mulberry apical cuttings were treated with different hormonal concentrations and placed in different rooting medium under shade house condition. After planting survival per cent, sprouting per cent, rooting per cent and shoot length of the plants were examined. Among the various interaction effect, IBA at 3000 ppm treated plants placed in Soil: Coir pith: FYM medium performed well in all parameters.

Impact of Various IBA Concentrations on Rooting in Apical Shoot Cutting and Stem Cuttings of Callistemon lanceolatus (Sm.) Sweet in Polytunnel Conditions

The study was undertaken to identify the optimal concentration of IBA and type of cuttings suitable for propagation of Callistemon lanceolatus and it was conducted at the Vanavarayar Institute of Agriculture, Pollachi, Coimbatore, India from December 2022 to September 2023. The experiment addressed the challenges of seed propagation, such as their seed capsules need to be activated by fire and the unpredictable nature of seed germination, which results in high progeny diversity due to genetic recombination. The investigation focused on apical and stem cuttings subjected to fifteen different treatments with three replications each; the Tukey test was used for statistical analysis; the cuttings were treated with various concentrations of Indole-3-butyric acid (IBA) and placed under polytunnel conditions; observations were made after 45 days, measuring sprouting percentage, rooting percentage, mean number of leaves, shoot length, mean number of roots, and root length per cutting. In light of these issues, the study explored clonal propagation as an alternative for mass multiplication of elite trees. The results of the study depicted that the maximum rooting percentage and root length were observed in T6 (73.28±8.87%) and (12.54±0.00cm) due to the exogenous application of auxin. On the whole, the result of this investigation suggest that apical shoot cuttings with IBA at 6000ppm can be made to induce maximum rooting and to produce high quality planting stock material for clonal propagation in Callistemon lanceolatus.

Stek Pucuk Kentang (Solanum tuberosum L.) Kultivar IPB CP1 Dapat Menghasilkan Umbi G0 di Dalam Rumah Kaca dan Rumah Kasa di Dataran Menengah

IPB CP1 potato is a raw material for potato chips. The advantage of IPB CP1 potatoes is their high yield. Challenges in potato production are limited areas with optimal altitude and condition as well as low quality and quantity of seeds. Utilizing a mid-altitude area for potato cultivation is one of the solutions to increase potato seed production. This study aimed to compare the growth and production of IPB CP1 G0 seed tuber in a greenhouse and screenhouse in mid-altitude with different numbers of shoot cuttings per polybag. This research was conducted with two factors. The first factor is the use of growth plant houses, i.e. greenhouse and screenhouse. The second factor is the number of shoot cuttings per polybag, i.e. 2 and 3 shoot cuttings per polybag. Growth parameters observed included plant height, number of nodes and leaves, and fresh and dry weight of shoots. The production parameters observed included the number as well as fresh and dry weight of tubers. The growth and production of IPB CP1 potatoes grown in the greenhouse were better than those grown in the screenhouse. The number of cuttings per polybag did not affect the growth and production of IPB CP1 potato.

Growth regulators on shooting and adventitious rooting of Mimosa caesalpiniifolia adult stem cuttings

Mimosa caesalpiniifolia, crucial for fencing and firewood in Brazil's Caatinga dry forest, presents a promising avenue for commercial propagation. The challenges faced in seed propagation, such as the scarcity of inputs and the difficulties in overcoming dormancy, highlight the advantages of vegetative methods. These methods emerge as a viable alternative, enabling uniform and large-scale production of clonal plantlets. This technique ensures consistent plant quality, which is crucial given the wide variety of uses for this species. Additionally, the use of plant growth regulators is critical to enhance the success of vegetative propagation, promoting the formation of robust roots and overall development of plants from cuttings of adult trees. Recognizing the significance of creating clonal forests of M. caesalpiniifolia and the necessity for understanding methods to expedite this endeavor, this study was designed to assess the shooting and adventitious rooting of stem cuttings sourced from adult M. caesalpiniifolia trees, examining the effects of auxinic agents. M. caesalpiniifolia cuttings were harvested through vegetative rescue from six randomly selected adult trees, treated with indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) at concentrations of 0, 1,000, 2,000, and 4000 mg L−1, using a completely randomized design in a 2 × 4 factorial arrangement. The evaluations included assessing root presence, number of shoots, vigor of clonal plantlets and plant survival under greenhouse conditions, shade house conditions, and full sun exposure. The study did not reveal any significant interaction between growth regulators and their concentrations under these conditions. IBA at intermediate concentrations enhanced root growth, while cuttings without growth regulators exhibited superior aerial development. A decrease in survival percentages with increasing concentrations of regulators was observed, while cuttings without regulators demonstrated better morphological development. The study suggests for large-scale nurseries, natural rooting and shoot formation in M. caesalpiniifolia cuttings may suffice for successful propagation without IBA or IAA.

Effect of auxins on biochemical composition of apical cuttings of Mulberry (Morus indica l.) Using mini clonal technology

A research study was carried out to examine the effects of auxin and rooting hormone on the biochemical composition of the mini clonal mulberry leaves raised using mini-clonal technique of (Morus indica L.) variety 'V1'.Two hormones, such as indole-3-butyric acid (IBA) and napthalene acetic acid (NAA), were used in the experiment. The hormones were replicated four times in each of the following concentration ranges: 1000, 2000, 3000, 4000, and 5000 ppm. Different hormone concentrations were applied to apical shoot cuttings before they were planted in the appropriate rooting medium in a greenhouse condition. The biochemical parameters analysed during the research study include leaf moisturecontent, nitrogen, phosphorous, potassium, chlorophyll-a, chlorophyll-b, carotenoid, total chlorophyll, crude protein, total carbohydrates, crude fat, total protein content present in mulberry leaves. The nutritive value of mulberry varieties depends on its biochemical constituents present in it. The results indicated that V1 mulberry mini clones have recorded better biochemical constituents and suitable for rearing of mulberry silkworms to get successful cocoon crop.

Comparison of the efficiency of synthetic auxins and biostimulants and two types of substrate in rooting of shoot cuttings in ‘Pi-ku 1’ rootstock

The low-cost propagation of semi-dwarf cherry rootstock is an essential issue in the production of maiden trees of this species. Among the promising rootstocks is ‘Pi-ku 1’, obtained in Germany. However, the possibility of its propagation using shoot cuttings has not been investigated. It was the purpose of this study. Two ways of cuttings treatment before placing them in the substrate were assessed. One used two preparations in powder (Rhizopon AA and Ukorzeniacz AB), and the second used two alcoholic auxin solutions (IAA and IBA). Instead of synthetic auxins, foliar spraying with two biostimulants was performed (Goteo and Bispeed). All the applied treatments increased the rooting percentage of ‘Pi-ku 1’ rootstock cuttings compared to the control from 5% for Rhizopon AA to 18.2% for auxin IBA. The exception was the lack of a positive effect of the Bispeed biostimulant (less than 5.2%). The use of synthetic auxins increased the number of utting roots more than three times (IBA) or almost twice (Ukorzeniacz AB) and their length more than twofold (IAA, Rhizopon AA) in proportion to the control. The effect of synthetic auxin treatments on the remaining growth parameters under study was also positive. Foliar treatment of cuttings with biostimulants did not change their growth. Only in one of the two years of the study did the fresh mass of cuttings improve after using Goteo biostimulant (7.5%). As part of the experiment, the effect of two substrate types – peat mixed with perlite and peat with sand – was also tested. During the two years of research, the cuttings were rooted several percent better in peat and sand (2.7% – 2018 and 4.4% – 2019). Using peat with sand as a rooting substrate significantly improved the number and length of roots (11.90 and 125.10) of Pi-ku 1 rootstock cuttings compared to the second one (9.23 and 109.08, respectively). All treatments applied to cuttings, except two biostimulants, increased the amount of chlorophyll in the leaves.

The Effect Of Shoot Cuttings And Vermiwash Concentration On Orchid Growth (Onchidium sphacelatum Lindl.) Aeroponically

The Oncidium sphacelatum orchid, commonly propagated vegetatively through pseudobulb separation, presents opportunities for further research into propagation methods and organic fertilizer application. This study, conducted in Batu City, aimed to explore the interaction between vermiwash concentration and shoot number on aeroponically cultivated Orchid sphacelatum Lind cuttings, as well as individual treatment effects. Employing a factorial RKLT design with shoot number (one or two) and vermiwash concentration (100, 200, or 300 ml/L) as factors, the study evaluated plant height, leaf count, stem length, shoot length, root count, and root length as variables. Data analysis involved ANOVA and BNJ tests. Results revealed that cuttings with two shoots and a 200 ml/L vermiwash concentration promoted optimal leaf growth. The number of shoots influenced shoot length, with two shoots demonstrating superior growth compared to single shoot cuttings. Interestingly, vermiwash concentration did not significantly impact shoot growth parameters, indicating that its effects may be limited in this context. This study sheds light on the potential benefits of using vermiwash in orchid cultivation, particularly in enhancing leaf growth when combined with multiple shoot cuttings. However, further investigation is needed to understand the nuanced effects of vermiwash on other growth aspects and its interaction with different propagation methods.Overall, the findings contribute to the optimization of orchid propagation techniques, providing valuable insights for orchid growers seeking to enhance plant growth and productivity sustainably. Further research could delve deeper into the mechanisms underlying the observed effects and explore additional variables to refine cultivation practices for Oncidium sphacelatum orchids.

EFFICIENT CLONAL PROPAGATION OF GUAVA CULTIVARS BY MINI SOFTWOOD CUTTINGS

Guava (Psidium guajava L.) is commercially propagated by seeds in Punjab and vegetative propagation systems are lacking. Propagation by stem cuttings is handicapped by the non-availability of plant material in bulk for cuttings and season dependency. Hence, juvenile mini softwood (MSW) shoot cuttings (3"-4") of Gola (Round) cultivar having two to three nodes were treated with plant growth regulators (PGRs) including indole butyric acid ‘IBA’ or naphthalene acetic acid ‘NAA’ in combinations with benzyl aminopurine ‘BAP’ and planted in sand, silt and coco peat (40:40:20 v/v) under CRD layout for PGR optimization. Cuttings treated with BAP and IBA (2 mgL-1 + 3 mgL-1) started early sprouting in 8 days and induced a greater number of branches (5-6), number of leaves (11), number of roots (6-7) and long roots (14 cm). Whereas, sprout length was higher up to 14 cm in cuttings treated with BAP and IBA (3 mgL-1 + 1 mgL-1). In the second part of the study, application of the optimized PGR treatment of BAP and IBA (2 mgL-1 + 3 mgL-1) to MSW cuttings of different commercial strains of cultivars Gola (Round) and Surahi (Pyriform) showed a better number of roots (5, 4), root length (13 cm, 11 cm), bud sprouting (100% and 80%) and number of nodes (5 each) in Gola (Sadabahar ‘G1’) and Surahi (Sindhi Bao ‘S2’), respectively. The rooted cuttings were maintained under standard growth room conditions and higher humidity till proper plant growth and the established plantlets were transferred to green house. This study established an efficient clonal propagation system using MSW cuttings thus reducing the plant material and PGRs required for mass multiplication of elite guava cultivars. Keywords: Auxin, cytokinin, softwood cuttings, shoot, rooting.

The Growth Response of Rendeu (<i>Staurogyne elongata</i> (Neese) Kuntze) to Shoot Pruning and Its Propagation by Shoot Cutting

Rendeu (Staurogyne elongata (Neese) Kuntze) is a native Indonesian plant used as food and traditional medicine in the daily life of the people residing around Gunung Halimun-Salak National Park. Due to the potential source of herbal-based medicines and traditional food in the long-run purposes, the proper method of its propagation is required, so that Rendeu can be conserved and utilised sustainably. This study employed two research designs. First, a completely randomized design with pruning and IAA (indole-3 acetic acid) treatment was used for seedling growths. Second, plant propagation applied a factorial randomized block design: planting media types and plant growth regulator (PGR) (rootone F) treatment. Observation included the number of buds, number of leaves, number of flowers, plant biomass, root length, and relative chlorophyll content using the SPAD tool. The data were analysed using ANOVA (SPSS ver. 17.0), followed by Pearson correlation analysis. The results showed that applying IAA and leaf pruning could increase the number of buds, the number of leaves and the fresh weight of S. elongata plants compared to the control plant. The addition of rootone increased the growth of Rendeu shoot cuttings, shown in all growth parameters and chlorophyll content. Humus was the best media for Rendeu’s growth among all planting medium. Planting media affected the increase in the number of leaves and the number of buds of S. elongata significantly. The interaction of planting media and PGR somewhat influenced root length and total leaf chlorophyll. The growth and production of S. elongata increased with the time of planting.

The longevity of cut <i>Polygonatum multiflorum</i> (L.) All. shoots depending on postharvest handling

The experiment was carried out to investigate the possibility of extension of the postharvest longevity of cut shoots of <i>Polygonatum multiflorum</i> depending on the type of conditioning. The shoots were collected for three experiments in May, June, and July. The following substances were used for conditioning: gibberellic acid (in May, June, and July) at a concentration of 50 and 100 mg dm<sup>-3</sup>, benzyladenine (in May) at a concentration of 50 or 100 mg dm<sup>-3</sup>, and 8-hydroxyquinoline sulfate (in July) at a concentration of 200 mg dm<sup>-3</sup>. The conditioning was carried out at a temperature of 5 °C or 18 °C (May, June) or 18 °C (July). After conditioning, shoots were stored in a room at a temperature of 18 °C (May, June) or at 18 °C or 22 °C (July). The shoots of <i>Polygonatum multiflorum</i> harvested in July and conditioned with gibberellic acid at a concentration of 100 mg dm<sup>-3</sup> were characterized by extended longevity. Benzyladenine at a concentration of 50 mg dm<sup>-3</sup> proved to be useful for conditioning. In turn, 8-hydroxyquinoline sulfate had no influence on the longevity of the shoots. The variation in the temperature during conditioning and storage was found not to have a positive impact on longevity.

Applications of Natural Rooting Inducer and Immersion Time on Root Growth of Chrysanthemum Shoot Cuttings

Chrysanthemum is a cut ornamental plant whose production in Indonesia in 2021 occupies the highest position namely 344 million compared to other ornamental plants. Production of chrysanthemum seedlings using the shoot cuttings method requires growth regulators to support the rooting process. It is necessary to use natural growth regulators which are cheaper and easier to obtain to encourage cutting root growth. This study aims to determine the appropriate natural root induction substances and immersion time for root induction of shoot cuttings of Chrysanthemum morifolium sp. This research was conducted at Kulon Progo Regency, Special Region of Yogyakarta, Indonesia. The study was conducted using a single-factor experimental method and arranged in a Completely Randomized Design with 3 replications and 5 samples for each replication. The treatments tested were 80% shallot extract, 100% coconut water, and 100% rice water with an immersion time of 5, 15, and 25 minutes respectively, and commercial rooting induction substances-Rootone F® which was applied and left for 5, 15 and 15 minutes. Parameters observed were the percentage of viable cuttings, plant height increase, the number of leaves increased, percentage of rooted cuttings, number of roots, root length, shoot fresh weight, shoot dry weight, root fresh weight, and root dry weight. Observations were analysed using analysis of variance at the α level of 5%. If there is a significant difference between the treatments tested, then a further test is carried out using Duncan‘s Multiple Range Test. The results showed that 80% shallot extract with 15 minutes of immersion could replace the use of Rootone-F® and showed the best relative results on the parameters of the number of roots, root fresh weight, and root dry weight of Chrysanthemum morifolium cuttings.

Effect of Improved Planting Stock on Tree Growth, Wood Properties, and Soil Fertility of Teak Plantations 10 Years After Planting

Teak (Tectona grandis Linn. f.) is one of the most economically valuable types of tropical forest because teak wood has high durability, resilience and good aesthetic qualities. Teak tree improvement in Indonesia was initiated in 1983 producing planting stock for reforestation, i.e. clonal seed orchards (CSO) to produce seedling as sexual reproduction (referred to seedling), and hedge orchards to produce shoot cuttings as asexual (vegetative) reproductive (referred to shoot cuttings). Teak plantations established using seedling and shoot cuttings should improve forest productivity. The objectives of this study were to compare the growth rate, wood properties, and soil fertility of teak plantations propagated using seedling and shoot cuttings. Three square plots of 100 trees (10 × 10 trees; 1,000 m2) were established on both types of plantation and tree growth characteristics, wood properties, and soil fertility were monitored. The tree growth characteristics were diameter at breast height (DBH), tree height (H), clear bole height (CB), and volume of standing stock (Vss), and the wood properties included pilodyn penetration (PP) and stress wave velocity (SWV). Soil fertility was assessed from soil samples collected from three soil depths, with three replicates in each plot: 0–5 cm (top layer), 6–30 cm (middle layer), and 31–60 cm (bottom layer). Soil samples were analysed for total nitrogen, total phosphorus, total potassium, total calcium, and total magnesium. The results showed that the mean DBH, H, CB, and Vss differed significantly between the type of planting stock. The DBH, H, CB, and Vss values were 28.4%, 46.8%, 49.1%, and 130.1% higher, respectively, in teak plantations from shoot cuttings than from seedling. Moreover, the SWV of shoot cuttings planting stock plantation was 3.6% higher than from seedling. The mean PP did not differ significantly between teak plantations from shoot cuttings (22.00 mm) and seedling (21.80 mm). Furthermore, the planting stock did not affect soil fertility, which was highest in the top layer in all treatments. Our results suggest that teak plantations from shoot cuttings would increase growth, forest productivity, maintaining wood quality and soil fertility. Thus, it can maintain sustainable teak forest plantations.

Post-aseptic adaptation and ex vitro propagation of Ukrainian cultivars of Paulownia Sieb. et Zucc.

Plantation forestry using highly productive and fast-growing plants involves the use of high-quality improved planting material for genetic constancy, which is solved by microclonal reproduction. However, the high survival rate and stability of plants obtained in vitro are realised as a result of post-aseptic adaptation of regenerants. Therefore, the research aims to improve the survival techniques of paulownia regenerants of the Ukrainian varieties “Feniks” and “Enerdzhy” ex vitro. The study was conducted in December 2021 on the Ukrainian paulownia varieties “Feniks” and “Enerdzhy”, cultivar “Feniks”. The survival rate, development of regenerants, and their damage by facultative saprophytic pathogens differed on substrates of organic (Eco Plus, La Flora, Jiffy, coconut peat, cotton wool) and mineral (vermiculite, perlite, sand) origin. The biological products “Rise P” and “Prestop” proved to be effective for the control of pathogenic microorganisms on substrates of organic origin. The technological feasibility of using perlite substrate is substantiated. The pathophysiology of ex vitro regenerants of paulownia under Fusarium is described. The thickness of the films used for sheltering influenced the regeneration, engraftment, microbial damage, and temperature of the wet chambers. Growing regenerants with covering films of different thicknesses showed a high survival rate in the variants with stretch films of 10 and 23 µm thickness. In the case of using polyethene films with thicknesses of 60, 80 and 100 µm, a sharp increase in temperature was observed, which was 48, 53 and 650C, respectively. At these high temperatures, most of the regenerants died, after which they were colonised by facultative saprophytes, and the tissues were completely macerated within two days. The optimal ratio of juvenility reduction and the acquisition of adaptive traits is characteristic of the second, third and fourth generation ex vitro. The expediency of propagation by stem cuttings up to the fourth generation, which preserves the regenerative ability of shoot cuttings, was substantiated. The practical results of the research are a protocol for growing paulownia plants in vitro and ex vitro on different types of substrates for use by institutions engaged in paulownia propagation

Urgent propagation of the endemic and possibly extinct in the wild tree Cassine koordersii (Celastraceae)

Cassine koordersii Kosterm. (Celastraceae) is a narrow endemic tree species known only to Mt. Puger, Jember, Indonesia. The IUCN Red List classifies it as Critically Endangered (CR) possibly extinct in the wild. Currently, there are only two living collections at the Bogor Botanic Gardens. As an urgent measure to anticipate extinction, vegetative propagation through shoot cuttings was conducted. This study aimed to determine the effects of rooting media and Rootone-F hormone on the successfulness of shoot cuttings of C. koordersii. It was carried out at the glass house in April 2022 with a 3-months observations period. The Komatsu-FORDA Fog Cooling (KOFFCO) system was adopted. The research used a completely randomized factorial design with rooting media and Rootone-F hormone as treatments. The media were sand (M1), cocopeat+burnt husk 2:1 (M2), and perlite+vermiculite 1:1 (M3). The Rootone-F hormone treatments included control (R1) and the addition of Rootone-F (R2). Six treatment combinations and four replications by 12 units obtained a total of 288 cuttings. The results indicate that in general, M2 and R2 have the best average yield: percentage of rooted cuttings (14.58%), number of primary roots (1.7), primary root length (1.58 cm), and percentage of survival cuttings (78.12%). The interaction between rooting media and Rootone-F hormone was not significant for all parameters. Despite only few new individuals are generated, they are valuable material for ex-situ collection backup. In addition, new juvenile seedlings are designed as mother trees for clonal orchards. Their high percentage rooting is expected to support the reintroduction program to their natural habitats.