Custom-made 3D Research Articles

Development of silk microfiber-reinforced bioink for muscle tissue engineering and in situ printing by a handheld 3D printer

Volumetric muscle loss (VML) presents a significant challenge in tissue engineering due to the irreparable nature of extensive muscle injuries. In this study, we propose a novel approach for VML treatment using a bioink composed of silk microfiber-reinforced silk fibroin (SF) hydrogel. The engineered scaffolds are predesigned to provide structural support and fiber alignment to promote tissue regeneration in situ. We also validated our custom-made handheld 3D printer performance and showcased its potential applications for in situ printing using robotics. The fiber contents of SF and gelatin ink were varied from 1 to 5 %. Silk fibroin microfibers reinforced ink offered increased viscosity of the gel, which enhanced the shape fidelity and mechanical strength of the bulk scaffold. The fiber-reinforced bioink also demonstrated better cell-biomaterial interaction upon printing. The handheld 3D printer enabled the precise and on-demand fabrication of scaffolds directly at the defect site, for personalized and minimally invasive treatment. This innovative approach holds promise for addressing the challenges associated with VML treatment and advancing the field of regenerative medicine.

Revision Cranioplasty with Three-dimensional Custom-made Titanium Implants in Patients with Failed Cranioplasty

Graft failure is a common complication of cranioplasty. Revision cranioplasty is required to overcome this complication. However, no previous studies have reported outcomes in revision cranioplasty with 3-dimensional (3D) custom-made titanium implants. We describe our experience with 3D titanium implants in patients with revision cranioplasty. We evaluated 43 consecutive patients between January 2011 and December 2019 who underwent revision cranioplasty using 3D custom-made titanium implants. The 3D image of the patient's cranium and the plan to close the cranium defect were created in a virtual environment using software programs. Demographic and radiologic features were compared based on the materials used in the initial cranioplasty. Previous material was autologous graft (AG) in 27 patients and polymethyl methacrylate (PMMA) in 16 patients. The mean time without revision cranioplasty is longer in patients with PMMA implants than in patients with AG. There was no statistically significant difference in the length of hospital stay between patients with PMMA implants and patients with AG. There were no postoperative adverse events such as infection, wound dehiscence, convulsions, or epidural hematoma in 38 patients during hospitalization. Wound dehiscence developed in 5 patients and surgical repair was required in one. Initial cranioplasty with PMMA provides a longer time period than the AG before the revision. However, both of them have similar outcomes based on length of hospital stay and cranial defect area. Custom-made 3D titanium implant is a good option for revision cranioplasty to prevent implant failure and reduce patients' cosmetic concerns.

Linear bone gain and healing complication rate comparative outcomes following ridge augmentation with custom 3D printed titanium mesh vs Ti-reinforced dPTFE. A randomized clinical trial.

The aim of this randomized clinical trial was to compare the qualitative (linear alveolar ridge changes) and quantitative (healing complications) outcomes after guided bone regeneration (GBR) using a custom-made 3D printed titanium mesh versus titanium reinforced dense PTFE membrane for vertical and horizontal augmentation of deficient alveolar ridges.

3DGEN: a framework for generating custom-made synthetic 3D datasets for civil structure health monitoring

The availability of high-quality datasets is increasingly critical in the field of computer vision-based civil structural health monitoring, where deep learning approaches have gained prominence. However, the lack of specialized datasets for such tasks poses a significant challenge for training a reliable model. To address this challenge, a framework, 3DGEN, is proposed to swiftly generate realistic synthetic 3D datasets which can be targeted for specific tasks. The framework is based on diverse 3D civil structural models, rendering them from various angles and providing depth information and camera parameters for training neural networks. By employing mathematical methods, such as analytical solutions and/or numerical simulations, deformation of civil engineering structures can be generated, ensuring a reliable representation of their real-world shapes and characteristics in the 3D datasets. For texture generation, a generative 3D texturing method enables users to specify desired textures using plain English sentences. Two successful experiments are conducted to (1) assess the efficiency of generating the 3D datasets using two distinct structures, (2) train a monocular depth estimation network to perform 3D surface reconstruction with the generated dataset. Notably, 3DGEN is not limited to 3D surface reconstruction; it can also be used for training neural networks for various other tasks. The code and dataset are available at: https://github.com/YANDA-SHAO/Beam-Dataset-SE

Utility of customized 3D compression mask with pressure sensors on facial burn scars: A single-blinded, randomized controlled trial

PurposeA pressure of approximately 15–25 mmHg is used for effective compression therapy to prevent and treat hypertrophic scar formation in patients with burns. However, conventional facial compression garments present challenges owing to inadequate pressure distribution in curved areas such as the cheeks, around the mouth, and the slope of the nose. This study aimed to evaluate the utility of a custom-made 3D compression mask equipped with pressure sensors to treat facial burn scars. MethodsThis single-blinded, prospective randomized controlled trial was conducted between May and October 2023, involving 48 burn scars in 12 inpatients with facial burns. We created the custom-made 3D compression mask equipped with pressure sensors, inner lined with biocompatible silicon, and a harness system using 3D printing technology, which can continuously monitor whether an appropriate pressure of 15–25 mmHg maintains. The biological scar properties, Vancouver Scar Scale (VSS), and Patient and Observer Scar Assessment Scale (POSAS) scores in patients with facial burns were assessed before applying the compression mask and garment and at 4 and 12 weeks after application. ResultsPre-application assessment of biological scar properties, VSS, and POSAS revealed no statistically significant differences between the 3D mask and control groups (p > 0.05 for all). Throughout the 12-week application, skin hydration and scar thickness significantly increased (p < 0.001) and reduced (p = 0.010), respectively, in the 3D mask group compared to the control group. Additionally, significant improvements in scar pliability (p = 0.004) and height (p = 0.009) of VSS, itching (p = 0.047), scar stiffness (p = 0.001), thickness (p = 0.011), and irregularity (p < 0.001) of POSAS-patient component, and scar thickness (p = 0.001), pliability (p = 0.012), and surface area (p = 0.027) of the POSAS-observer component were observed in 3D mask group throughout the 12-week application compared to the control group. ConclusionThe customized 3D compression mask equipped with pressure sensors significantly improved scar thickness, skin hydration, and various assessment scale parameters throughout the 12-week application.

In situ crosslinkable multi-functional and cell-responsive alginate 3D matrix via thiol-maleimide click chemistry

In vivo, cells interact with the extracellular matrix (ECM), which provides a multitude of biophysical and biochemical signals that modulate cellular behavior. Inspired by this, we explored a new methodology to develop a more physiomimetic polysaccharide-based matrix for 3D cell culture. Maleimide-modified alginate (AlgM) derivatives were successfully synthesized using DMTMM to activate carboxylic groups. Thiol-terminated cell-adhesion peptides were tethered to the hydrogel network to promote integrin binding. Rapid and efficient in situ hydrogel formation was promoted by thiol-Michael addition “click” chemistry via maleimide reaction with thiol-flanked protease-sensitive peptides. Alginate derivatives were further ionically crosslinked by divalent ions present in the medium, which led to greater stability and allowed longer cell culture periods. By tailoring alginate's biofunctionality we improved cell-cell and cell-matrix interactions, providing an ECM-like 3D microenvironment. We were able to systematically and independently vary biochemical and biophysical parameters to elicit specific cell responses, creating custom-made 3D matrices. DMTMM-mediated maleimide incorporation is a promising approach to synthesizing AlgM derivatives that can be leveraged to produce ECM-like matrices for a broad range of applications, from in vitro tissue modeling to tissue regeneration.

Three-Dimensional Printed Filters Based on Poly(ethylene glycol) Diacrylate Hydrogels Doped with Silver Nanoparticles for Removing Hg(II) Ions from Water.

Nowadays, due to water pollution, more and more living beings are exposed to dangerous compounds, which can lead to them contracting diseases. The removal of contaminants (including heavy metals) from water is, therefore, a necessary aspect to guarantee the well-being of living beings. Among the most used techniques, the employment of adsorbent materials is certainly advantageous, as they are easy to synthesize and are cheap. In this work, poly(ethylene glycol) diacrylate (PEGDA) hydrogels doped with silver nanoparticles (AgNPs) for removing Hg(II) ions from water are presented. AgNPs were embedded in PEGDA-based matrices by using a photo-polymerizable solution. By exploiting a custom-made 3D printer, the filters were synthesized. The kinetics of interaction was studied, revealing that the adsorption equilibrium is achieved in 8 h. Subsequently, the adsorption isotherms of PEGDA doped with AgNPs towards Hg(II) ions were studied at different temperatures (4 °C, 25 °C, and 50 °C). In all cases, the best isotherm model was the Langmuir one (revealing that the chemisorption is the driving process and the most favorable one), with maximum adsorption capacities equal to 0.55, 0.57, and 0.61 mg/g, respectively. Finally, the removal efficiency was evaluated for the three temperatures, obtaining for 4 °C, 25 °C, and 50 °C the values 94%, 94%, and 86%, respectively.

Modelling cell shape in 3D structured environments: A quantitative comparison with experiments.

Cell shape plays a fundamental role in many biological processes, including adhesion, migration, division and development, but it is not clear which shape model best predicts three-dimensional cell shape in structured environments. Here, we compare different modelling approaches with experimental data. The shapes of single mesenchymal cells cultured in custom-made 3D scaffolds were compared by a Fourier method with surfaces that minimize area under the given adhesion and volume constraints. For the minimized surface model, we found marked differences to the experimentally observed cell shapes, which necessitated the use of more advanced shape models. We used different variants of the cellular Potts model, which effectively includes both surface and bulk contributions. The simulations revealed that the Hamiltonian with linear area energy outperformed the elastic area constraint in accurately modelling the 3D shapes of cells in structured environments. Explicit modelling the nucleus did not improve the accuracy of the simulated cell shapes. Overall, our work identifies effective methods for accurately modelling cellular shapes in complex environments.

Relationship between pectoralis major stiffness and shoulder extension range of motion.

This study aimed to investigate the correlation between the passive muscle stiffness of the pectoralis major muscle pars clavicularis (PMc) and shoulder extension range of motion (ROM) in both male and female participants. Thirty-nine (23 male/16 female) physically active and healthy participants volunteered in this study. After a standardized warm-up, the PMc stiffness was tested via shear wave elastography at a slightly stretched position (long muscle length) and in a non-stretched position (short muscle length). Additionally, a custom-made device and 3D motion capture assessed the active shoulder extension ROM. We found a significant moderate and negative relationship between shoulder extension ROM and PMc stiffness at long muscle length (rs = -0.33; p = 0.04) but not at short muscle length (r = -0.23; p = 0.17). Additionally, there was no significant difference between male and female participants in the correlation analyses at both elbow angles. The moderate correlation between PMc stiffness at a slightly stretched position and shoulder extension ROM suggests that additionally, other structures such as nerves/fascia stiffness or even stretch tolerance might be factors that can be related to shoulder extension ROM.

Mechanical loading of ex vivo bovine trabecular bone in 3D printed bioreactor chambers

Previous ex vivo bone culture methods have successfully implemented polycarbonate (PC) bioreactors to investigate bone adaptation to mechanical load; however, they are difficult to fabricate and have been limited to a 5 mm maximum specimen height. The objective of this study was to validate a custom-made 3D printed MED610TM bioreactor system that addresses the limitations of the PC bioreactor and assess its efficacy in ex vivo bone culture. Twenty-three viable trabecular bone cores (10 mm height by 10 mm diameter) from an 18-month-old bovine sternum were cultured in MED610TM bioreactors with culture medium at 37 °C and 5% CO2 for 21-days. Bone cores were ranked based on their day 0 apparent elastic modulus (Eapp) and evenly separated into a “Load” group (n = 12) and a control group (n = 11). The Load group was loaded five times per week with a sinusoidal strain waveform between −1000 and −5000 με for 120 cycles at 2 Hz. Eapp was assessed on day 0, 8, and 21 using quasi-static tests with a −4000 με applied strain. Over 21-days, the Eapp of Load group samples tended to increase by more than double the control group (53.4% versus 20.9%) and no visual culture contamination was observed. This study demonstrated that bone organ culture in 3D printed MED610TM bioreactors replicated Eapp trends found in previous studies with PC bioreactors. However, further studies are warranted with a larger sample size to increase statistical power and histology to assess cell viability and bone mineral apposition rate.

Automatic detection of nostril and key markers in images

In the medical field, one of complex challenges in surgery involving the skin, cartilage, mucosa, and the skeletal platform is the cleft nasal deformity. The causes of deformity are nasal formed from improper fusion of the medial and lateral nasal prominences with the maxillary prominence during embryologic development. Primary rhinoplasty or first time of cleft nose repair is the nasal surgery for repairing the cleft nasal deformity. In post cheiloplasty, the primary rhinoplasty patients must use nasal splint for supporting surgical wound. However, regular nasal splints are expensive and only come with default nasal size. This limits nasal splint from supporting specific patient cases such as children. The goal of this study is to implement a program that can automatically detect the nostril and green marker in patient images for creating custom-made 3D nasal splints. The proposed method is created utilizing the CNN model. YOLO architecture is utilized because it is the one of several CNN architectures that works well with the face recognition task. The YOLOV5, YOLOV8, and YOLO-NAS are attempted and compared in the training phase. The model with a highest performance is selected, and fine-tuned for adjusting to be compatible with a patient dataset. The fine-tuned YOLOV8 reaches a mAP with 99.5%. The predicted images from fine-tuned model are used to perform body part segmentation like Otsu's thresholding and discover contour to locate essential features like the green marker and nostril in ellipse and bounding boxes. The nostril distance is calculated using the bounding box and ellipse. The columella distance or nostril gap is measured from the distance between nostrils in YOLO predicted label. Both distances are converted into centimeter scale and evaluated with ground truth value in volunteer and patient cases from nasal expert for inspecting efficiency between bounding box, ellipse, and size of marker. The result proves that the proposed model can detect crucial features with mean absolute error 0.102. Furthermore, the proposed model indicates that marker size has no effect on detection and distance, according to the findings of the experiments.

Effect of fiber content on mechanical properties of carbon fiber-reinforced polyether-ether-ketone composites prepared using screw extrusion-based online mixing 3D printing

The mechanical properties of carbon fiber-reinforced polyether-ether-ketone (CF/PEEK) composites are closely related to the fiber content. However, the low fiber content (typically below 20 wt%) that can be prepared by current material extrusion limits the application of the composites. In this work, CF/PEEK composites with up to 50 wt% carbon fiber content were manufactured using a custom-made screw extrusion-based online mixing 3D printhead. The impact of fiber loading on the rheological and flow characteristics of the composites was thoroughly examined initially. Subsequently, the influence of fiber content on the crystallinity and mechanical behavior of 3D-printed parts before and after annealing was further explored. The results indicated that adding CF and annealing significantly enhanced the strength and modulus of the 3D-printed composites within a fiber content range of 10 wt% to 40 wt%, compared to those of pure PEEK. At 40 wt% CF, the tensile and flexural strengths of the samples reached their maximum values at 135.9 MPa and 251.2 MPa, respectively, which were enhanced by 94% and 111% compared to those of the unannealed pure PEEK specimens. Also, these properties are the highest values achieved in 3D-printed short CF/PEEK composites. The mechanical properties of composites are affected by various factors such as fiber content, crystallinity, and internal pore defects when the fiber content is less than 20 wt%. However, when the fiber content exceeds 20 wt%, the influence of crystallinity on the mechanical properties becomes less significant. This study further explores the potential application of CF/PEEK composites and provides a novel approach for composite preparation.

Designing and manufacturing custom-made 3D printed assistive devices: A comparison of three workflows

BACKGROUND: Do-it-yourself technologies such as 3D printing offer interesting opportunities for creating custom-made assistive devices for people with disabilities. Although these opportunities are increasingly acknowledged, it remains unclear how 3D printing technology should be implemented in assistive devices delivery processes. OBJECTIVE: In three separate action research projects carried out in Belgium, Italy and The Netherlands, workflows were designed and evaluated that incorporate 3D printing as a standard option for providing clients with suitable assistive devices. METHODS: In this paper we describe and compare the three workflows that were conceived simultaneously, yet independently from each other. RESULTS: Based on the evaluations of these workflows, and the experiences of the researchers who developed the workflows, we provide recommendations for implementing 3D printing as a common approach in assistive device delivery processes in practice. Most importantly, designing and manufacturing should be done by means of a client-centered co-creation process by interdisciplinary teams of clinicians, clients, and 3D printing experts. We provide several recommendations for facilitating and supporting collaborations within such teams. CONCLUSIONS: The three workflows presented in this paper are strikingly similar and therefore provide a convincing starting point for interdisciplinary design teams who wish to embark on 3D printing custom-made assistive devices.

Application of raycast method for person geolocalization and distance determination using UAV images in Real-World land search and rescue scenarios

People enjoy spending time in the wilderness for numerous reasons. However, they occasionally get lost or injured, and their survival depends on being efficiently found and rescued in the shortest possible time. A search and rescue operation (SAR) is launched after the accident is reported, and all possible resources are activated. The inclusion of drones in SAR operations has enabled the use of computer vision methods to detect persons in aerial imagery automatically. When searching by drone, preference is given to oblique photographs that cover a larger area within a single image, reducing the search time. Unlike vertical photographs, oblique photographs include a significant scale change, making it challenging to locate a person in the real world and determine their distance from the drone. In order to solve this problem, encouraged by our previous successful simulations, we explored the possibility of applying the raycast method for person geolocalization and distance determination for use in real-world scenarios. In this paper, we propose a system able to precisely geolocate persons automatically detected in offline processed images recorded during the SAR mission. After a series of experiments on terrains of different configurations and complexity, using a custom-made 3D terrain generator and raycaster, along with a deep neural network-based person detector trained on our custom dataset, we defined a method for geolocating detected person based on raycast, which allows using low-cost commercial drones with a monocular camera and no Real-Time Kinematic module while enabling laser rangefinder emulation during offline image analysis. Our person geolocating method overcomes the problems faced by previous methods and, using a single flight sequence with only 4 consecutive detections, significantly outperforms the previous best results, with reliability of 42,85% (geolocating error of 0.7 m on recording from a 30 m height). Also, a short time of only 247 s enables offline processing of data recorded during a 21-minute drone flight covering approximately an area of 10 ha, proving that the proposed method can be effectively used in actual SAR missions. We also proposed a new evaluation metric (ErrDist) for person geolocalization and provided recommendations for using the proposed system for person detection and geolocation in real-world scenarios.

3D photopolymerized microstructured scaffolds influence nuclear deformation, nucleo/cytoskeletal protein organization, and gene regulation in mesenchymal stem cells.

Mechanical stimuli from the extracellular environment affect cell morphology and functionality. Recently, we reported that mesenchymal stem cells (MSCs) grown in a custom-made 3D microscaffold, the Nichoid, are able to express higher levels of stemness markers. In fact, the Nichoid is an interesting device for autologous MSC expansion in clinical translation and would appear to regulate gene activity by altering intracellular force transmission. To corroborate this hypothesis, we investigated mechanotransduction-related nuclear mechanisms, and we also treated spread cells with a drug that destroys the actin cytoskeleton. We observed a roundish nuclear shape in MSCs cultured in the Nichoid and correlated the nuclear curvature with the import of transcription factors. We observed a more homogeneous euchromatin distribution in cells cultured in the Nichoid with respect to the Flat sample, corresponding to a standard glass coverslip. These results suggest a different gene regulation, which we confirmed by an RNA-seq analysis that revealed the dysregulation of 1843 genes. We also observed a low structured lamina mesh, which, according to the implemented molecular dynamic simulations, indicates reduced damping activity, thus supporting the hypothesis of low intracellular force transmission. Also, our investigations regarding lamin expression and spatial organization support the hypothesis that the gene dysregulation induced by the Nichoid is mainly related to a reduction in force transmission. In conclusion, our findings revealing the Nichoid's effects on MSC behavior is a step forward in the control of stem cells via mechanical manipulation, thus paving the way to new strategies for MSC translation to clinical applications.

Custom-made 3D printing-based cranioplasty using a silicone mould and PMMA

All types of cranioplasty techniques restore the morphology of the skull and affect patient aesthetics. Safe and easy techniques are required to enhance patients’ recovery and the rehabilitation process. We propose a new method of cranioplasty. The 3-dimensional (3D) reconstruction of a thin-layer computed tomography (CT) scan of the skull was used to reflect the intact side onto the defect and subtract the overlapping points from one another. In this way, a 3D model of the planned implant can be built in the required shape and size. The precise fit of the implant can be checked by printing the defective part of the skull in case it can be modified. A sterilisable silicone mould based on the finalized model was created afterwards. Polymethyl methacrylate implants were prepared directly in an aseptic environment in the operating room during surgery. Between 2005 and 2020, we performed 54 cranioplasties on 52 patients whose craniotomies were performed previously for indications of traumatic brain injury, stroke or tumour surgeries. No technical problems were noted during the operations. In 2 cases, septic complications that occurred were not connected to the technique itself, and the implants were removed and later replaced. Our proposed technique based on 3D-printed individual silicone moulds is a reliable, safe, easily reproducible and low-cost method to repair different skull defects.

Probing charge transfer through antifouling polymer brushes by electrochemical methods: The impact of supporting self-assembled monolayer chain length

Ultrathin surface-tethered polymer brushes represent attractive platforms for a wide range of sensing applications in strategically vital areas such as medicine, forensics, or security. The recent trends in such developments towards “real world conditions” highlighted the role of zwitterionic poly(carboxybetaine) (pCB) brushes which provide excellent antifouling properties combined with bio-functionalization capacity. Highly dense pCB brushes are usually prepared by the “grafting from” polymerization triggered by initiators on self-assembled monolayers (SAMs). Here, multi-methodological experimental studies are pursued to elucidate the impact of the alkanethiolate SAM chain length (C6, C8 and C11) on structural and functional properties of antifouling poly(carboxybetaine methacrylamide) (pCBMAA) brush. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in a custom-made 3D printed cell employing [Ru(NH3)6]3+/2+ redox probe were used to investigate penetrability of SAM/pCBMAA bilayers for small molecules and interfacial charge transfer characteristics. The biofouling resistance of pCBMAA brushes was characterized by surface plasmon resonance; ellipsometry and FT-IRRAS spectroscopy were used to determine swelling and relative density of the brushes synthesized from initiator-bearing SAMs with varied carbon chain length. The SAM length was found to have a substantial impact on all studied characteristics; the highest value of charge transfer resistance (Rct) was observed for denser pCBMAA on longer-chain (C11) SAM when compared to shorter (C8/C6) SAMs. The observed high value of Rct for C11 implies a limitation for the analytical performance of electrochemical sensing methods. At the same time, the pCBMAA brushes on C11 SAM exhibited the best bio-fouling resistance among inspected systems. This demonstrates that proper selection of supporting structures for brushes is critical in the design of these assemblies for biosensing applications.

STRUCTURAL DEFECTS AND VARIABLE POROSITY IN 3D-PRINTED CUPS FROM SIX MANUFACTURERS

3D printing is rapidly being adopted by manufacturers to produce orthopaedic implants. There is a risk however of structural defects which may impact mechanical integrity. There are also no established standards to guide the design of bone-facing porous structures, meaning that manufacturers may employ different approaches to this. Characterisation of these variables in final-production implants will help understanding of the impact of these on their clinical performance.We analysed 12 unused, final-production custom-made 3D printed acetabular cups that had been produced by 6 orthopaedic manufacturers. We performed high resolution micro-CT imaging of each cup to characterise the morphometric features of the porous layers: (1) the level of porosity, (2) pore size, (3) thickness of porous struts and (4) the depth of the porous layers. We then examined the internal cup structures to identify the presence of any defects and to characterise: (1) their total number, (2) volume, (3) sphericity, (4) size and (5) location.There was a variability between designs in the level of porosity (34% to 85%), pore size (0.74 to 1.87mm), strut thickness (0.28 to 0.65mm), and porous layer depth (0.57 to 11.51mm). One manufacturer printed different porous structures between the cup body and flanges; another manufacturer printed two differing porous regions within the cup body.5 cups contained a median (range) of 90 (58–101) defects. The median defect volume was 5.17 (1.05–17.33) mm3. The median defect sphericity and size were 0.47 (0.19–0.65) and 0.64 (0.27–8.82) mm respectively. The defects were predominantly located adjacent to screw holes, within flanges and at the transition between the flange and main cup body; these were between 0.17 and 4.66mm from the cup surfaces.There is a wide variability between manufacturers in the porous titanium structures they 3D print. The size, shape and location of the structural defects identified are such that there may be an increased risk of crack initiation from them, potentially leading to a fracture. Regulators, surgeons, and manufacturers should be aware of this variability in final print quality.

The second harmonic heating system for the NORTH tokamak

A substantial contribution in the understanding of fusion plasmas can be achieved by conducting experiments in simplified plasma devices. One of those devices is NORTH, located in Denmark. Part of the research activity on NORTH is focused on non-linear interaction of microwaves with toroidally magnetized plasmas. An additional microwave heating system was recently installed in the device. The source can deliver 800 W of 5.8 GHz microwaves, which are coupled into the plasma by a custom-made 3D printed horn antenna. The antenna design was validated by numerical modelling and experimental measurements. Here we discuss this validation, and describe the system and its commissioning. Additionally, we present a newly developed diagnostic to monitor the plasma response in the frequency range up to 6 GHz, based on a fast 12.5 GS/s digitizer. By exploiting this system, we have characterized the new source. Furthermore, we have preliminary investigated the interaction of the 5.8 GHz microwave with the plasma, and we present first observations of wave coupling in NORTH.

IMPLEMENTATION OF ROBOTIC AND ASSISTIVE TECHNOLOGIES IN THE PATIENTCENTERED PHYSICAL REHABILITATION

There is a necessity for a consistent framework for the integration of the robotic and assistive technologies for the purposes of the rehabilitation which covers the entire spectrum (preventative, restorative, supportive, palliative rehabilitation) and explores all aspects of the of the recovery process – physical, emotional, and mental while allowing for real-time adjustments and flexibility based on the individual patients’ needs. In this paper we propose a set of tools for a rehabilitation solution that provides personalized care for the patients from the diagnose to the process of active recovery while measuring and evaluating the individual’s condition after surgery, injury or a trauma to restore and maximize the previous function. The main objective for implementing the assistive technologies into the physical therapy is to personalize the process of recovery based on the specific requirements of the patients. To achieve this, physical, emotional, and virtual devices are utilized mitigate and improve the physical and emotional challenges for the patients. In parallel with the implementation of the robotic technologies, custom-made 3D printed devices are also introduced and explored. To evaluate the effectiveness of the program, a 3-step methodology is applied – 1) diagnose of the patient, 2) implementation of the assistive technologies, 3) tracking the progress and making the necessary adjustments to the process and/or the devices.