Curcumin Yield Research Articles

Isolation of curcumin from Lakadong turmeric of Meghalaya and development of its PLGA-Cur-NS loaded nanogel for potential anti-inflammatory and cutaneous wound healing activity in Wistar rats

BackgroundLakadong turmeric (LKD) from Meghalaya, India, boasts high curcumin levels, but lacks scientific study. Moreover, Curcumin's poor solubility hinders its clinical use in inflammation and wound healing. This study isolated curcumin from LKD, forming a PLGA nanosuspension-based nanogel and explored for its potential anti-inflammatory and cutaneous wound healing activity in Wistar rats.MethodologyThe LKD rhizome was successively extracted by soxhlet using chloroform, hexane, ethyl acetate and methanol and their total phenolic content and antioxidant property was determined. Chloroform extract was loaded in column chromatography and curcumin was isolated, purified and characterized by FTIR, NMR, DSC, MS and HPTLC and its purity was analyzed by HPLC. The isolated curcumin was loaded into PLGA nanosuspensions by nanoprecipitation method followed by conversion to carbopol based Nanogel. The nanogels were characterized physicochemically and the drug permeation was observed. The optimized nanogel was evaluated for its anti-inflammatory and cutaneous wound healing activity by carrageenan-induced paw edema and excision wound model in albino Wistar rats divided in five groups and treated with plain carbopol gel, marketed turmeric cream and Cur NS nanogel (0.6% w/w equivalent curcumin each).ResultsResults depicted that the chloroform extract showed the presence of a considerable amount of phenolics with 50-60% yield of curcumin. HPLC analysis depicted that the LKD curcumin had higher purity when compared to standard curcumin. Homogenous curcumin nanosuspensions were obtained with size 150 ± 50 nm and PDI 0.2 ± 0.1. The Cur-NS-nanogel showed satisfactory physical property, drug loading and stability. Ex vivo permeation studies in rats showed controlled permeation with steady state flux of 21.28 ± 0.23 µg/cm2/hr in 24 h. Topical intervention with Cur-NS-nanogel (0.6%) significantly (p < 0.05) decreased the paw volume by 43.97% in the 3rd hour and 72.19% on the 4th hour. Furthermore, the cutaneous wound healing property of Cur-NS-nanogel when compared to negative control rats showed a substantial (p < 0.05) reduction in the percentage wound contraction from 5 to 20th days.ConclusionLKD showed high curcumin content with significant antioxidant effects. LKD curcumin loaded into stable nanogel depicted safe and controlled skin permeability with promising cutaneous wound healing and anti-inflammatory activity in experimental rats.

Efficacy of green solvents in pungent, aroma, and color extractions of spice oleoresins and impact on phytochemical and antioxidant capacities

The current study investigated the effect of green solvents (ethanol, acetone, and their mixture) on oleoresin yield, extraction of pungent compounds, volatiles, colorants, and the correlations with phytochemical and antioxidant activities of 5 spices (chili, black pepper, turmeric, ginger, and coriander). The solvents’ polarities and boiling points determined the oleoresin extractability, and the solvent mixture recorded the maximum yield in all spices. A total of 33 volatiles and 8 non-volatile compounds were identified in the essential oil and oleoresin fractions. Curcumin and 6-gingerol contributed to strong antioxidant properties with Pearson correlations of 0.999 and 0.924, respectively. Acetone reported the highest yield of curcumin (244.09 ± 1.71 mg/g) and 6-gingerol (131.98 ± 2.86 mg/g), whereas ethanol was the most effective solvent for capsaicinoids and piperine. Chili oleoresin extracted by ethanol delivered the maximum pungency. Acetone displayed the highest extractability of linalool detected in coriander oleoresin and essential oil. Chili oleoresin contained the highest carotenoid yield, and acetone extracted the maximum red and yellow fractions. Chili and black pepper possessed high Browning Indices and positively correlated with the total flavonoid content. β-caryophyllene dominated the essential oil profile of chili and black pepper, and the solvent mixture reported the maximum yields as 47.43% ± 0.69% and 75.11% ± 0.64%, respectively. Conclusively, the solvent mixture extracted the maximum oleoresin yield; acetone showed the highest affinity for colorants, volatiles, and antioxidants in turmeric, ginger, and coriander, while ethanol was the most efficient solvent in extracting bioactive compounds of chili and black pepper.

Extraction, purification and anti-cancer activity of curcumin

Turmeric has long been used as a strong anti-inflammatory in Indian drug systems. Turmeric is rich in curcuminoids that have different chemical, physico-chemical properties. Current work reports exraction of curcuminoids, curcumin using the Soxhlet extractor. Purification and quantification of curcumin is performed by chromatography. The yield percentage of curcumin reported 4.09% by the Soxhlet extraction method. Different solvent were used for extraction, among them acetone extract was with high yield of curcumin. Individual curcumin extract analysed using UV-VIS Spectroscopy and HPLC. Our results showed that the Salem turmeric contains highest percentage of curcumin. The in vitroMTT assay on the U87MG glioma cell line demanstrated anti-cancer activity of isolated curcumin.

Lasered Graphene Microheaters Modified with Phase-Change Composites: New Approach to Smart Patch Drug Delivery.

The combination of paraffin wax and O,O′-bis(2-aminopropyl) polypropylene glycol–block–polyethylene glycol–block–polypropylene glycol was used as a phase-change material (PCM) for the controlled delivery of curcumin. The PCM was combined with a graphene-based heater derived from the laser scribing of polyimide film. This assembly provides a new approach to a smart patch through which release can be electronically controlled, allowing repetitive dosing. Rather than relying on passive diffusion, delivery is induced and terminated through the controlled heating of the PCM with transfer only occurring when the PCM transitions from solid to liquid. The material properties of the device and release characteristics of the strategy under repetitive dosing are critically assessed. The delivery yield of curcumin was found to be 3.5 µg (4.5 µg/cm2) per 3 min thermal cycle.

Quantification of Curcumin Obtained from Supercritical Carbon Dioxide Extraction of Curcuma domestica Valet. Rhizomes

Background: Curcumin is well known bioactive compound available in Curcuma domestica Valet i.e. turmeric. The research work was carried with intention to get maximum yield of medicinally active constituents (Curcumin) of Curcuma domestica Valet. rhizomes through supercritical fluid extraction. Methods: The experiments were conducted in the year 2013-14. Process parameters namely pressure, temperature, flow rate and particle size were optimized using a Taguchi method. During investigation, L9 orthogonal array experimental design was followed. Result: The optimized values of pressure, temperature, flow rate and particle size obtained were 35 MPa, 40°C, 25 g/min and 0.246 mm, respectively. Significant improvement in the yield of curcumin and extract was obtained under optimum operating conditions.

Extraction of curcumin from turmeric by ultrasonic-assisted extraction, identification, and evaluation of the biological activity

Introduction: Dried rhizomes of turmeric have been traditionally used as a medicinal herb, dietary spice, food source, food preservative, and natural coloring agent in many Asian countries. This study aimed to develop the ultrasonic-assisted extraction (UAE) method for the extraction of curcumin from turmeric powder, evaluate the extraction efficiency, curcumin concentration, and biological activities.Methods: The UAE effects were examined based on several parameters of extraction efficiencies. The curcumin content was also determined using high-performance liquid chromatography (HPLC), and the total phenolic content (TPC) was estimated by Folin-Ciocalteu colorimetric method. The antibacterial activity of the extracted was evaluated against the test pathogenic bacteria by the disc diffusion method. The correlation between extraction yield and curcumin content was performed by principal components analysis (PCA).Results: The optimal UAE conditions were: ethanol, a solid-liquid ratio of 1:10 (w/v), an extraction time of 40 min, and only one extraction step. Under the optimal conditions, the yield of curcumin was 160.3 ± 1.17 (mg/g extract) and the TPC was 185.5 ± 3.07 (mg gallic acid equivalent /g extract). PCA presented the positive correlation between curcumin and the TPC of the studied extracts. Comparison of antibacterial activity between UAE and maceration method against the tested bacteria showed no significant difference at P &gt; 0.05.Conclusion: UAE was a viable alternative as a rapid, efficient, and simple means of extraction of curcumin from turmeric. The extracts had great potential as a source of antioxidant agents with high amounts of curcuminoids, phenolic compounds and exhibited activity against pathogenic bacteria.

Production of curcumin by engineered Escherichia coli

Curcumin is exclusively isolated from Zingiberaceae plants with a broad spectrum of bioactivities. In the present study, we used the diketide-CoA synthase (DCS) and curcumin synthase (CURS) genes to construct a non-natural fusion gene encoding diketide-CoA synthase::curcumin synthase (DCS::CURS). This fusion protein, together with the acetyl coenzyme A carboxylase (ACC) and the 4-coumarate coenzyme A ligase (4CL), were introduced into Escherichia coli for the production of curcumin from ferulic acid. The process is divided into two stages, the growth stage using LB medium and the fermentation stage using the modified M9 medium. The yield of curcumin reached 386.8 mg/L by optimizing the induction of protein expression in the growth stage, and optimizing the inoculum volume, medium composition and fermentation time in the fermentation stage, as well as the addition of macroporous resin AB-8 into the second medium to attenuate the toxicity of the end product. The exploitation of the non-natural fusion protein DCS::CURS for the production of curcumin provides a new alternative to further promoting the production of curcumin and the related analogues.

A new synthetic biology approach for the production of curcumin and its glucoside in Atropa belladonna hairy roots

Curcumin has ignited global interest as an elite drugable molecule, owing to its time-honoured pharmacological activities against diverse human ailments. Limited natural accessibility and poor oral bioavailability caused major hurdles in the curcumin-based drug development process. We report the first successful testimony of curcumin and its glucoside synthesis in Atropa belladonna hairy roots (HR) through metabolic engineering. Re-routing the inherent biosynthetic precursors of the phenylpropanoid pathway of A. belladonna by heterologous expression of key curcumin biosynthetic pathway genes (i.e., Diketide-CoA synthase-DCS and Curcumin synthase-CURS3) and glucosyltransferase gene (CaUGT2) resulted in the production of curcumin and its glucoside in HR clones. Under shake-flask cultivation, the PGD2-HR1clone bearing DCS/ CURS3 genes showed the maximum curcumin yield (180.62 ± 4.7 μg/g DW), while the highest content of curcumin monoglucoside (32.63 ± 2.27 μg/g DW) along with curcumin (67.89 ± 2.56 μg/g DW) were noted in the PGD3-HR3 clone co-expressing DCS/CURS3 and CaUGT2 genes. Bioreactor up-scaling showed yield improvements in the PGD2-HR1 (2.3 fold curcumin) and the PGD3-HR3 clone (0.9 and 1.65 folds of curcumin-monoglucoside and curcumin respectively). These findings proved the advantageous use of HR cultures as the production source for curcumin and its glucoside, which remained unexplored so far.

Principal Component Analysis Based Solvent Map for Optimisation of Rate and Yield of Curcumin Synthesis

Curcumin, a diarylheptanoid normally isolated from Curcuma longa with multiple bioactivities, was synthesized from vanillin and pentane-1,4-dione as initial substrates, n-butylamine as a catalyst, boron oxide as a protecting agent, and tri-n-butyl borate as a water scavenger. The solvents play crucial roles in the reaction rate and the yield of curcumin synthesis. Applying principal component analysis (PCA) method on 89 molecular descriptors calculated for a set of 272 organic solvents, a two-dimensional solvent map was established. Two first principal components, which were found to characterize polarity and polarizability of solvents, accounted for 60% of the data variation and were used as the ordinates of the solvent map. Five solvents, including n-hexane, N,N-dimethylacetamide, n-butyl acetate, chloroform and isopropanol, were selected from different areas of the solvent map to investigate the solvent effects on the rate and the yield of curcumin synthesis. The experimental results revealed that the highest formation rate and yield of curcumin were obtained in N,N-dimethylacetamide. The results offered direction for further explorations of the solvent map, in which N,N-dimethylacetamide should be used as a reference to find the optimum solvent for the synthetic procedure of curcumin.

Optimization extraction and purification of biological activity curcumin from Curcuma longa L by high-performance counter-current chromatography.

The extraction condition of curcumin from Curcuma longa L was optimized through four factors and three levels orthogonal experiment based on the results of single factor tests. Under the optimal conditions: theconcentrationofethanol 80%, extraction temperature 70°C, the ratio of liquid to material 20, and extraction time 3 h, a crude extract with the yield of curcumin 56.8mg/g could be obtained. The isolation and purification of curcuminoids from the crude extract was performed on high performance counter current chromatography employing an optimized solvent system n-hexane/ethyl acetate/methanol/water (2/3/3/1, v/v/v/v). From 97mg crude sample (in which the purity of curmumin was 68.56%), 67mg curmumin, 18mg demethoxycurcumin, and 9.7mg bisdemethoxycurcumin with a high-performance liquid chromatography purity of 98.26, 97.39, and 98.67%, respectively, were obtained within 70min. The antioxidant activities and cytotoxicity of purified curcumin was comparable to that of the commercial product, indicating that the biological activity of curcumin could be maintained by this method.

Application of a Novel Ionic Liquid as an Alternative Green Solvent for the Extraction of Curcumin from Turmeric with Response Surface Methodology: Determination and Optimization Study

Curcumin, a bio-active phytochemical, is a major component of turmeric. It is also a significant food flavoring and potential therapeutic agent for diverse diseases. The traditional organic solvents are not environmentally friendly because of their features including toxicity, volatility and flammability. For this reason, in this study, the ionic liquid 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl) imide ([BMIM][Tf2N]), as an alternative green solvent, was investigated for the extraction of curcumin from turmeric. The curcumin in the turmeric samples under various extraction conditions was determined by the use of high performance liquid chromatography (HPLC). Response surface methodology (RSM) was utilized to investigate the influence of extraction parameters including the time from 10 to 60 min, temperature from 25 to 55 °C, and solvent volume from 10 to 30 mL. The experimentally determined curcumin yield ranged between 0.76% and 2.94%. The optimal extraction conditions were obtained employing an extraction time interval equal to 60 min, temperature of 55 °C and solvent volume of 30 mL. The maximum curcumin yield extracted from turmeric was 2.94% using the optimal conditions.

Comparison of Curcuma domestica and Curcuma xanthorrhiza oleoresins extracted using maceration, Soxhlet, and ultrasound-assisted extraction (UAE)

This research aimed to use conventional and modern extraction methods for extraction of C. domestica and C. xanthorrhiza oleoresins. For this purpose, maceration, Soxhlet, and Ultrasound-Assisted Extraction (UAE) were implemented to extract oleoresins. All the extraction method used acetone as a solvent with the ratio of ground spices to solvent was 1:5 (w/w). The results showed that the Soxhlet extraction method gave the highest oleoresin yield of C. domestica (18.89 ± 0.68%) and C. xanthorrhiza (14.70 ± 0.62%). Maceration method gave the highest curcumin yield of 49.52 ± 0.63% and 15.82 ± 0.27% for C. domestica and C. xanthorrhiza oleoresins, respectively. Meanwhile, the oleoresin obtained from the UAE method gave the lowest residual solvent on both C. domestica (16.00 ± 4.67%) and C. xanthorrhiza (15.66 ± 3.33%) oleoresins. C. xanthorrhiza oleoresin obtained from maceration tends to show the best colour, while the best colour of C. domestica oleoresin obtained from UAE. Propylene glycol had the best solubility for C. domestica oleoresin, while C. xanthorrhiza oleoresin was soluble in all solvents.

Recent Advancement of Microbial Production of Curcuminoids and Its Industrial Applications: A Review

Turmeric (from Curcuma longa) is a yellow colored spice commonly used in daily diet. It has been extensively used in traditional medicine since ancient times to treat various nervous and disorder disease. A little was known about the biosynthesis curcuminoid in turmeric and identified the enzymes involved in the curcuminoid biosynthetic pathway and confirmed the involvement of the phenylpropanoid pathway in the production of these compounds in plants. Traditionally known curcumin has emerged as a modern biological regulator curcuminoids, agroclimatic and soil environmental variation are also influencing the curcumin synthase gene expression, which is correlated with curcumin yield in turmeric cultivars. Microbial production of curcuminoids is very promising and production yield can be improved by using synthetic biology approaches and metabolic engineering tools, to make heterologous production competitive with the current process of curcuminoid’s extraction from plants. Type III polyketide synthases are responsible for the production of curcuminoids. Among those DCS and CURS enzymes have been used to synthesize curcuminoids. Synthetic biology and metabolite engineering approaches have generated microbial cell factories that can allow the for the mass production of pharmaceutically and nutraceuticals important microbial metabolites in an environmentally friendly and efficient way. Considering a wide pharmaceutical, industrial and health beneficial applications of curcuminoids, this review focused on microbial production of curcuminoids and substrate recognizing and regulatory mechanism of curcuminoid synthase and obtain the mutant enzymes using mutagenesis study and synthetic biology approaches.

The use of curcumin in modeling gerontological drinks with enhanced flavor

In connection with the emerging global trend of an increase in the number of elderly people, an increase in morbidity and age-related changes occurring in the body, including a deterioration in taste and olfactory sensitivity, there is a need to provide rational nutrition for this category of citizens. The aim of the study was to model the formulations of gerontological drinks with enhanced flavor with a low glycemic index, with enhanced functional properties and containing the stevioside-curcumin complex.The study was carried out in the city of Yekaterinburg in three stages: investigated the taste and olfactory sensitivity in people aged 25-40 years and 65-90 years, investigated the effect of stevia extracts on the solubility of curcumin, simulated samples of drinks. It was found that the recognition of tastes in people in the age group of 65-90 years is worse in comparison with people in the age group of 25-40 years: for salty - by 37.8%, for sour - by 39.0%, for bitter - by 64 , 0%, for sweets by 6.0%. Olfactory sensitivity is 25.9% worse. Disease sensitivity is significantly affected by the disease: sensitivity is worse in diseases of the digestive system by 22.9%, circulatory system - by 26.8%, endocrine system - by 25.2%, genitourinary system - by 26.1%. To enhance the flavor intensity, yeast extract and the stevioside-curcumin complex obtained by extraction of curcumin from turmeric rhizome powder with long ethyl alcohol using stevioside were added to the drinks. It was found that with the addition of stevioside in an amount of 8%, the maximum yield of curcumin was observed — 14.33 ± 0.3 mg / ml. Then they developed model samples of drinks, the sweetness profile of which is close to that of sugar, the aftertaste is 3 times longer, the taste intensity is 15% higher, antioxidant activity is 5.7 times more than that of a competitor.

Shade, Establishment Method, and Varietal Effects on Rhizome Yield and Curcumin Content in Turmeric in Alabama

ABSTRACTGrowing interest in curcumin from turmeric (Curcuma longa L.) for medical and health purposes has led to interest in turmeric cultivation in the United States. The warm growing season in Alabama is short relative to tropical environments where the plant is normally grown, and research on cultural practices is lacking. A split‐split‐plot experiment was performed in Alabama to assess the effects of shade (40% shade vs. no shade), establishment method (early establishment in greenhouse over heat vs. direct seeding), and variety on rhizome yield and curcumin content. Canonical discriminant analysis revealed distinctive trends based on varietal differences, establishment methods, and presence or absence of shade. Extending the growing season by early establishment in the greenhouse increased rhizome and curcumin yield, as well as curcumin concentration in lateral rhizomes. Shade increased plant height, leaf size, and fresh weight of rhizomes, but an apparent increase in rhizome dry weight was not significant. Shade decreased curcumin concentration and yield in mother rhizomes but had little effect on curcumin concentration in lateral rhizomes. An apparent increase in curcumin yield in lateral rhizomes with shade was not significant. Significant differences among varieties were observed for rhizome yield and curcumin concentration and yield. Cultivation of turmeric in Alabama is feasible by extending the growing season and selection of short‐season varieties high in curcumin.

Production of the plant polyketide curcumin in Aspergillus oryzae: strengthening malonyl-CoA supply for yield improvement.

The filamentous fungus Aspergillus oryzae was recently used as a heterologous host for fungal secondary metabolite production. Here, we aimed to produce the plant polyketide curcumin in A. oryzae. Curcumin is synthesized from feruloyl-coenzyme A (CoA) and malonyl-CoA by curcuminoid synthase (CUS). A. oryzae expressing CUS produced curcumin (64 μg/plate) on an agar medium containing feruloyl-N-acetylcysteamine (a feruloyl-CoA analog). To increase curcumin yield, we attempted to strengthen the supply of malonyl-CoA using two approaches: enhancement of the reaction catalyzed by acetyl-CoA carboxylase (ACC), which produces malonyl-CoA from acetyl-CoA, and inactivation of the acetyl-CoA-consuming sterol biosynthesis pathway. Finally, we succeeded in increasing curcumin yield sixfold by the double disruption of snfA and SCAP; SnfA is a homolog of SNF1, which inhibits ACC activity by phosphorylation in Saccharomyces cerevisiae and SCAP is positively related to sterol biosynthesis in Aspergillus terreus. This study provided useful information for heterologous polyketide production in A. oryzae.

Ảnh hưởng của phân bón lá đến sinh trưởng, hàm lượng và năng suất Curcumin trên nghệ Xà cừ Curcuma xanthorrhiza Roxb.

The experiment was conducted in Binh Thuy District, Can Tho City to study the effects of phenylalanine, salicylic acid (SA), iron sulfate, zinc sulfate and borax on the growth and yield of Curcuma xanthorrhiza Roxb. turmeric in 2017-2018. The experiment was arranged in a randomized complete block design with 6 levels of plant growth regulators and micronutrients spraying (control, foliar spray of growth regulators Phe 100 ppm, SA 100 ppm, foliar spray of FeSO4 0.5%, ZnSO4 0.5% and borax 0.5% at 120 days after planting (DAP), with 3 replications. The results revealed that the significant number of tillers per clump, number of leaves per tiller, number of leaves per clump, plant height, leaf length and leaf width at the time of survey. At the time of harvesting, spraying FeSO4 0.5% or Phe 100 ppm recorded most effective, fresh weight of rhizomes per clump (467 and 443 g/clump, respectively), curcumin contents were equal (14.7%) and curcumin yield (17.0 and 17.2 g/clump, respectively).

Green Mass Production of Pure Nanodrugs via an Ice-Template-Assisted Strategy.

To make nanomedicine potentially applicable in a clinical setting, several methods have been developed to synthesize pure nanodrugs (PNDs) without using any additional inert carriers. In this work, we report a novel green, low-cost, and scalable ice-template-assisted approach which shows several unique characteristics. First, the whole process only requires adding a drug solution into an ice template and subsequent melting (or freeze-drying), allowing easy industrial mass production with low capital investment. Second, the production yield is much higher than that of the traditional reprecipitation approach. The yield of Curcumin (Cur) PNDs is over two orders (∼140 times) magnitude higher than that obtained in a typical reprecipitation preparation. By adjusting simple processing parameters, PNDs with different sizes (∼20-200 nm) can be controllably obtained. Finally, the present approach can be easily applicable for a wide range of hydrophobic therapeutic drugs without any structural modification.

Ultrasound assisted extraction of curcumin

Turmeric is known as “Golden Spice” used as food color. Curcumin content mainly depend on turmeric variety, method of extraction and extraction parameters viz. solvent and particle size. Three different varieties viz. Salem, Krishna, Rajapuri were used for experiment which gives 3.8%, 2.5%, 4.2% extraction yield, respectively. Among the different solvent used for extraction, ethanol shows maximum extraction yield of curcumin 3.8%, 2.5%, 4.2% in all three varieties respectively than other solvents. As per the effect of particle size concern, finer the particle size more the extracted curcumin. Different ultrasound power of 25w, 50w, 75w was used for extraction, Results showed that 25 w power yields more curcumin in Rajapuri (5.3%) variety, followed by Salem (4.8%) and Krishna (3.8%). Whereas curcumin yield extracted by conventional method was Rajapuri (4.6%), Salem (4.1%) andKrishna (2.8%), this was lesser than the yield extracted by ultrasound method. The new method can help to improve the curcumin extraction yield and thus help to improve the socio-economic status of small scale entrepreneurs.

Organic cultivation of high yielding turmeric (Curcuma longa L.) cultivars: a viable alternative to enhance rhizome productivity, profitability, quality and resource-use efficiency in monkey–menace areas of north-western Himalayas

Turmeric (Curcuma longa L.) is a major spice crop in India in general and north-western (NW) Himalayas in particular where it is grown mostly organic by default using biennial local strains having low productivity. This crop is becoming an alternate crop in monkey–menace areas in NW Himalayas transferring into gigantic fallow-lands otherwise. There is a dire need to boost turmeric productivity in the region by introducing high yielding turmeric cultivars (HYTCs). Thus, an extensive ‘On–farm’ experimentation was done at 10 locations in 6 monkey–menace affected community-development-blocks (CBDs) (Sadar, Sundernagar, Dharampur, Gopalpur, Seraj and Gohar) of the randomly selected district–Mandi of the randomly selected Himalayan province–Himachal Pradesh as the study area. For this purpose, two newly released annually harvested HYTCs (Palam Pitamber and Palam Lalima) alongwith a widely grown local strain ‘Suketi Haldi’ in NW Himalayas (as check), were planted alongwith organic manure i.e. FYM @ 20 t ha–1 on oven dry weight basis. Mean data of two years revealed that Palam Pitamber resulted in highest rhizome yield (32.94 t ha–1) followed by Palam Lalima (32.35 t ha–1) compared to biennially harvested ‘Suketi Haldi’ (12.45 t ha–1). Likewise, Palam Pitamber resulted in highest net returns and net B: C ratio (▪ 416,548 ha–1; 5.38) followed by Palam Lalima and Suketi Haldi, respectively. The curcumin content was higher in ‘Suketi Haldi’ (5.18%), but the curcumin yield was significantly higher in Palam Pitamber and Palam Lalima. The monetary-efficiency indices revealed that organic turmeric cultivation using HYTCs may fetch about 1243–1270 ▪ ha−1 day−1 to the Himalayan farmers. Overall, the turmeric rhizome yield was enhanced by about three–folds, profitability by 3–4 folds, resource-use efficiency (RUE) by 2–3 folds in terms of production– and monetary efficiency, water-use efficiency and partial factor productivity of applied nutrients (NPK) by the adoption of HYTCs coupled with FYM @ 20 t ha–1 over ‘Suketi Haldi’. Higher technology adoption rate (72–98%) and net income gains (2.3–2.5 folds) in the study area advocated for employing ‘Participatory-mode’ adaptive research methodologies following intensive technology transfer programme in remote hill agro-ecologies. In nutshell, the newly developed HYTCs exhibited great potential in boosting the turmeric productivity, profitability, curcumin yield and RUE vis-à-vis wider adaptability in monkey–menace areas to improve rural livelihoods and make organic turmeric cultivation a promising agri–entrepreneurship in NW Himalayas. [Display omitted]